1     M         1.882   1.883   1.915   2.314   2.313   2.368   2.037   2.038   2.079
2     K        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
3     V        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
4     K        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
5     A        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
6     T        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
7     K        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
8     L        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
9     R        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
10    I        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
11    K        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
12    Q        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
13    R        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
14    R        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
15    K        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
16    N        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
17    K        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
18    G        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
19    L        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
20    N        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
21    I        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
22    S         0.128   0.109   0.089   0.144   0.107   0.066   0.009  -0.020  -0.054
23    R        -1.146  -1.145  -1.122  -1.465  -1.448  -1.423  -1.004  -1.002  -0.960
24    L         0.128   0.139   0.097   0.121   0.145   0.032   0.009   0.027  -0.041
25    D        -1.146  -1.145  -1.122  -1.168  -1.165  -1.126  -1.004  -1.002  -0.960
26    I         1.882   1.883   1.915   2.017   2.016   2.071   2.037   2.038   2.079
27    I         0.128   0.139   0.097  -0.224  -0.204  -0.259   0.009   0.027  -0.041
28    R         0.128   0.139   0.097   0.414   0.435   0.365   0.009   0.027  -0.041
29    A        -0.028  -0.028  -0.001   0.157   0.157   0.204  -0.244  -0.243  -0.200
30    E        -0.028  -0.028  -0.001  -0.122  -0.124  -0.076  -0.244  -0.243  -0.200
31    M        -0.028  -0.028  -0.001   0.007   0.006   0.053  -0.244  -0.243  -0.200
32    D         1.882   1.883   1.915   1.928   1.926   1.981   2.037   2.038   2.079
33    V        -0.028  -0.028  -0.001   0.120   0.120   0.167  -0.244  -0.243  -0.200
34    V         1.882   1.883   1.915   2.158   2.158   2.212   2.037   2.038   2.079
35    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
36    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
37    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
38    P         1.882   1.883   1.915   2.178   2.177   2.232   2.037   2.038   2.079
39    S        -1.146  -1.145  -1.122  -0.835  -0.826  -0.792  -1.004  -1.002  -0.960
40    P         0.128   0.109   0.089   0.341   0.304   0.267   0.009  -0.020  -0.054
41    G         0.128   0.139   0.097   0.226   0.238   0.194   0.009   0.027  -0.041
42    L        -1.146  -1.145  -1.122  -1.284  -1.269  -1.242  -1.004  -1.002  -0.960
43    P         1.882   1.883   1.915   2.178   2.177   2.232   2.037   2.038   2.079
44    E         0.128   0.139   0.097  -0.224  -0.201  -0.285   0.009   0.027  -0.041
45    K        -0.028  -0.028  -0.001  -0.335  -0.334  -0.290  -0.244  -0.243  -0.200
46    V        -0.028  -0.028  -0.001   0.144   0.145   0.190  -0.244  -0.243  -0.200
47    N        -0.028  -0.028  -0.001  -0.214  -0.213  -0.168  -0.244  -0.243  -0.200
48    E        -0.028  -0.028  -0.001  -0.227  -0.226  -0.182  -0.244  -0.243  -0.200
49    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
50    K        -0.028  -0.028  -0.001  -0.148  -0.147  -0.102  -0.244  -0.243  -0.200
51    S        -0.028  -0.028  -0.001   0.205   0.207   0.252  -0.244  -0.243  -0.200
52    K        -1.146  -1.145  -1.122  -1.573  -1.570  -1.532  -1.004  -1.002  -0.960
53    N         0.128   0.139   0.097   0.073   0.085   0.021   0.009   0.027  -0.041
54    I         0.128   0.139   0.097   0.352   0.366   0.300   0.009   0.027  -0.041
55    P        -1.146  -1.145  -1.122  -1.292  -1.295  -1.250  -1.004  -1.002  -0.960
56    L         1.882   1.883   1.915   1.510   1.518   1.562   2.037   2.038   2.079
57    P         1.882   1.883   1.915   2.178   2.177   2.232   2.037   2.038   2.079
58    E        -1.146  -1.145  -1.122  -1.144  -1.143  -1.102  -1.004  -1.002  -0.960
59    G         0.128   0.139   0.097   0.341   0.353   0.305   0.009   0.027  -0.041
60    I         0.128   0.139   0.100   0.341   0.355   0.296   0.009   0.027  -0.036
61    N         0.128   0.139   0.100   0.132   0.146   0.076   0.009   0.027  -0.036
62    L         1.882   1.883   1.915   1.510   1.518   1.562   2.037   2.038   2.079
63    L         1.882   1.883   1.915   1.510   1.518   1.562   2.037   2.038   2.079
64    S         0.128   0.139   0.100   0.301   0.316   0.263   0.009   0.027  -0.036
65    S        -1.146  -1.145  -1.122  -0.791  -0.782  -0.747  -1.004  -1.002  -0.960
66    K         0.128   0.139   0.097   0.043   0.063  -0.032   0.009   0.027  -0.041
67    E         0.128   0.139   0.100  -0.086  -0.073  -0.137   0.009   0.027  -0.036
68    I         0.128   0.109   0.089   0.061   0.021  -0.046   0.009  -0.020  -0.054
69    I        -1.146  -1.145  -1.122  -1.326  -1.333  -1.284  -1.004  -1.002  -0.960
70    D        -1.146  -1.145  -1.122  -1.309  -1.316  -1.267  -1.004  -1.002  -0.960
71    L         1.882   1.883   1.915   1.510   1.518   1.562   2.037   2.038   2.079
72    I         0.128   0.139   0.100   0.061   0.085  -0.020   0.009   0.027  -0.036
73    Q         0.128   0.109   0.089   0.144   0.109   0.077   0.009  -0.020  -0.054
74    T         0.128   0.139   0.097   0.244   0.257   0.203   0.009   0.027  -0.041
75    H         0.128   0.139   0.100   0.409   0.427   0.350   0.009   0.027  -0.036
76    R         0.128   0.139   0.100   0.284   0.313   0.223   0.009   0.027  -0.036
77    H        -1.146  -1.145  -1.122  -0.784  -0.791  -0.740  -1.004  -1.002  -0.960
78    Q        -1.146  -1.145  -1.122  -1.176  -1.180  -1.134  -1.004  -1.002  -0.960
79    L         0.128   0.139   0.100  -0.440  -0.411  -0.498   0.009   0.027  -0.036
80    E         0.128   0.109   0.089   0.103   0.074   0.060   0.009  -0.020  -0.054
81    L        -0.028  -0.028  -0.001  -0.122  -0.124  -0.076  -0.244  -0.243  -0.200
82    Y         0.128   0.109   0.089   0.481   0.459   0.418   0.009  -0.020  -0.054
83    V        -1.146  -1.145  -1.122  -0.640  -0.636  -0.596  -1.004  -1.002  -0.960
84    T        -1.146  -1.145  -1.122  -1.002  -0.992  -0.959  -1.004  -1.002  -0.960
85    K        -1.146  -1.145  -1.122  -1.136  -1.122  -1.093  -1.004  -1.002  -0.960
86    F         0.128   0.109   0.089   0.497   0.467   0.443   0.009  -0.020  -0.054
87    N         0.128   0.109   0.089   0.132   0.093   0.054   0.009  -0.020  -0.054
88    P        -1.146  -1.145  -1.122  -1.017  -1.020  -0.975  -1.004  -1.002  -0.960
89    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
90    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
91    L        -1.146  -1.145  -1.122  -1.152  -1.141  -1.110  -1.004  -1.002  -0.960
92    T        -1.146  -1.145  -1.122  -1.088  -1.096  -1.045  -1.004  -1.002  -0.960
93    D         0.128   0.109   0.089   0.150   0.119   0.097   0.009  -0.020  -0.054
94    F         0.128   0.139   0.097   0.369   0.384   0.323   0.009   0.027  -0.041
95    A        -1.146  -1.145  -1.122  -0.806  -0.803  -0.762  -1.004  -1.002  -0.960
96    G        -1.146  -1.145  -1.122  -0.986  -0.997  -0.943  -1.004  -1.002  -0.960
97    K         0.128   0.109   0.089  -0.224  -0.253  -0.300   0.009  -0.020  -0.054
98    I        -1.146  -1.145  -1.122  -0.902  -0.903  -0.859  -1.004  -1.002  -0.960
99    H        -1.146  -1.145  -1.122  -1.002  -1.007  -0.959  -1.004  -1.002  -0.960
100   A         0.128   0.109   0.089   0.425   0.399   0.362   0.009  -0.020  -0.054
101   F        -1.146  -1.145  -1.122  -1.002  -1.011  -0.959  -1.004  -1.002  -0.960
102   R         0.128   0.139   0.097  -0.030  -0.003  -0.066   0.009   0.027  -0.041
103   D        -1.146  -1.145  -1.122  -1.017  -1.021  -0.975  -1.004  -1.002  -0.960
104   Q         1.882   1.883   1.915   1.963   1.961   2.016   2.037   2.038   2.079
105   F         0.128   0.139   0.100   0.442   0.457   0.398   0.009   0.027  -0.036
106   K         0.128   0.139   0.100  -0.224  -0.201  -0.280   0.009   0.027  -0.036
107   Q         0.128   0.109   0.089   0.037  -0.003  -0.047   0.009  -0.020  -0.054
108   L         1.882   1.883   1.915   1.510   1.518   1.562   2.037   2.038   2.079
109   E         0.128   0.139   0.100   0.025   0.036  -0.013   0.009   0.027  -0.036
110   E         1.882   1.883   1.915   1.780   1.777   1.833   2.037   2.038   2.079
111   N        -1.146  -1.145  -1.122  -1.041  -1.046  -0.998  -1.004  -1.002  -0.960
112   F         0.128   0.139   0.100   0.144   0.169   0.062   0.009   0.027  -0.036
113   E        -1.146  -1.145  -1.122  -1.184  -1.186  -1.142  -1.004  -1.002  -0.960
114   D         0.128   0.139   0.097  -0.173  -0.151  -0.228   0.009   0.027  -0.041
115   L         1.882   1.883   1.915   1.510   1.518   1.562   2.037   2.038   2.079
116   H        -1.146  -1.145  -1.122  -0.918  -0.927  -0.874  -1.004  -1.002  -0.960
117   E         0.128   0.109   0.089  -0.086  -0.123  -0.157   0.009  -0.020  -0.054
118   Q        -1.146  -1.145  -1.122  -0.880  -0.875  -0.837  -1.004  -1.002  -0.960
119   K         1.882   1.883   1.915   1.687   1.694   1.739   2.037   2.038   2.079
120   D        -1.146  -1.145  -1.122  -0.948  -0.940  -0.905  -1.004  -1.002  -0.960
121   K        -1.146  -1.145  -1.122  -1.002  -0.995  -0.959  -1.004  -1.002  -0.960
122   V        -1.146  -1.145  -1.122  -1.209  -1.200  -1.167  -1.004  -1.002  -0.960
123   Q         0.128   0.139   0.100  -0.006   0.015  -0.073   0.009   0.027  -0.036
124   A        -1.146  -1.145  -1.122  -0.820  -0.828  -0.777  -1.004  -1.002  -0.960
125   L        -1.146  -1.145  -1.122  -1.554  -1.546  -1.513  -1.004  -1.002  -0.960
126   L         0.128   0.109   0.089  -0.224  -0.244  -0.269   0.009  -0.020  -0.054
127   E        -1.146  -1.145  -1.122  -1.160  -1.156  -1.118  -1.004  -1.002  -0.960
128   N        -1.146  -1.145  -1.122  -1.184  -1.194  -1.142  -1.004  -1.002  -0.960
129   C        -1.146  -1.145  -1.122  -0.986  -0.996  -0.943  -1.004  -1.002  -0.960
130   R        -1.146  -1.145  -1.122  -1.064  -1.049  -1.022  -1.004  -1.002  -0.960
131   I         0.128   0.139   0.100   0.138   0.160   0.068   0.009   0.027  -0.036
132   L         1.882   1.883   1.915   1.510   1.518   1.562   2.037   2.038   2.079
133   E         1.882   1.883   1.915   1.780   1.777   1.833   2.037   2.038   2.079
134   S         0.128   0.139   0.100   0.290   0.304   0.250   0.009   0.027  -0.036
135   K         0.128   0.139   0.097  -0.180  -0.161  -0.249   0.009   0.027  -0.041
136   Y         1.882   1.883   1.915   2.241   2.252   2.294   2.037   2.038   2.079
137   V        -1.146  -1.145  -1.122  -0.668  -0.668  -0.624  -1.004  -1.002  -0.960
138   A         0.128   0.139   0.097  -0.024  -0.004  -0.059   0.009   0.027  -0.041
139   S         0.128   0.139   0.097  -0.104  -0.083  -0.150   0.009   0.027  -0.041
140   W         0.128   0.109   0.089   0.617   0.592   0.552   0.009  -0.020  -0.054
141   Q         1.882   1.883   1.915   1.963   1.961   2.016   2.037   2.038   2.079
142   D         0.128   0.109   0.089   0.073   0.037   0.008   0.009  -0.020  -0.054
143   Y         0.128   0.139   0.097  -0.161  -0.135  -0.187   0.009   0.027  -0.041
144   H        -1.146  -1.145  -1.122  -0.994  -0.998  -0.951  -1.004  -1.002  -0.960
145   S        -1.146  -1.145  -1.122  -1.386  -1.378  -1.344  -1.004  -1.002  -0.960
146   E         0.128   0.139   0.097  -0.394  -0.371  -0.451   0.009   0.027  -0.041
147   F         0.128   0.139   0.097   0.369   0.384   0.323   0.009   0.027  -0.041
148   S        -1.146  -1.145  -1.122  -0.940  -0.931  -0.897  -1.004  -1.002  -0.960
149   K         0.128   0.139   0.097   0.055   0.077  -0.020   0.009   0.027  -0.041
150   K         0.128   0.109   0.089  -0.111  -0.136  -0.169   0.009  -0.020  -0.054
151   Y         0.128   0.139   0.100   0.481   0.507   0.436   0.009   0.027  -0.036
152   G         0.128   0.139   0.097   0.352   0.366   0.316   0.009   0.027  -0.041
153   D        -1.146  -1.145  -1.122  -1.334  -1.338  -1.293  -1.004  -1.002  -0.960
154   I         0.128   0.139   0.097   0.121   0.133   0.075   0.009   0.027  -0.041
155   A         0.128   0.109   0.089   0.409   0.375   0.343   0.009  -0.020  -0.054
156   L         0.128   0.109   0.089  -0.186  -0.205  -0.226   0.009  -0.020  -0.054
157   K         1.882   1.883   1.915   1.687   1.694   1.739   2.037   2.038   2.079
158   K         0.128   0.109   0.089  -0.030  -0.044  -0.077   0.009  -0.020  -0.054
159   K        -1.146  -1.145  -1.122  -1.144  -1.131  -1.102  -1.004  -1.002  -0.960
160   L         0.128   0.109   0.089  -0.123  -0.139  -0.154   0.009  -0.020  -0.054
161   E        -1.146  -1.145  -1.122  -1.403  -1.400  -1.362  -1.004  -1.002  -0.960
162   Q        -1.146  -1.145  -1.122  -0.918  -0.921  -0.874  -1.004  -1.002  -0.960
163   N         0.128   0.109   0.089   0.043   0.004  -0.049   0.009  -0.020  -0.054
164   T        -1.146  -1.145  -1.122  -0.784  -0.785  -0.740  -1.004  -1.002  -0.960
165   K         0.128   0.139   0.100  -0.061  -0.040  -0.096   0.009   0.027  -0.036
166   K        -1.146  -1.145  -1.122  -1.403  -1.404  -1.362  -1.004  -1.002  -0.960
167   L         0.128   0.109   0.089  -0.276  -0.299  -0.329   0.009  -0.020  -0.054
168   D         0.128   0.109   0.089  -0.036  -0.076  -0.118   0.009  -0.020  -0.054
169   E         0.128   0.109   0.089  -0.049  -0.085  -0.114   0.009  -0.020  -0.054
170   E         0.128   0.139   0.097  -0.224  -0.201  -0.285   0.009   0.027  -0.041
171   S         0.128   0.109   0.089   0.381   0.354   0.337   0.009  -0.020  -0.054
172   S         0.128   0.109   0.089   0.144   0.107   0.066   0.009  -0.020  -0.054
173   Q        -1.146  -1.145  -1.122  -1.056  -1.065  -1.014  -1.004  -1.002  -0.960
174   L         0.128   0.139   0.100  -0.123  -0.104  -0.142   0.009   0.027  -0.036
175   E         0.128   0.139   0.100   0.037   0.047   0.000   0.009   0.027  -0.036
176   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
177   T         0.128   0.139   0.097   0.290   0.302   0.248   0.009   0.027  -0.041
178   T        -1.146  -1.145  -1.122  -0.948  -0.945  -0.905  -1.004  -1.002  -0.960
179   T        -1.146  -1.145  -1.122  -1.033  -1.037  -0.990  -1.004  -1.002  -0.960
180   R        -1.146  -1.145  -1.122  -0.661  -0.650  -0.617  -1.004  -1.002  -0.960
181   S         0.128   0.139   0.100   0.261   0.275   0.218   0.009   0.027  -0.036
182   I        -1.146  -1.145  -1.122  -1.152  -1.159  -1.110  -1.004  -1.002  -0.960
183   D         1.882   1.883   1.915   1.885   1.883   1.938   2.037   2.038   2.079
184   S        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
185   A        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
186   D        -0.028  -0.028  -0.001  -0.308  -0.307  -0.263  -0.244  -0.243  -0.200
187   D        -0.028  -0.028  -0.001  -0.161  -0.160  -0.115  -0.244  -0.243  -0.200
188   L         1.882   1.883   1.915   1.510   1.518   1.562   2.037   2.038   2.079
189   D         1.882   1.883   1.915   1.885   1.883   1.938   2.037   2.038   2.079
190   Q        -1.146  -1.145  -1.122  -1.309  -1.298  -1.267  -1.004  -1.002  -0.960
191   F         1.882   1.883   1.915   2.203   2.202   2.257   2.037   2.038   2.079
192   I         0.128   0.139   0.097  -0.224  -0.204  -0.259   0.009   0.027  -0.041
193   K         0.128   0.139   0.100  -0.224  -0.201  -0.280   0.009   0.027  -0.036
194   N        -1.146  -1.145  -1.122  -1.072  -1.075  -1.030  -1.004  -1.002  -0.960
195   Y         1.882   1.883   1.915   2.241   2.252   2.294   2.037   2.038   2.079
196   L        -1.146  -1.145  -1.122  -1.080  -1.067  -1.037  -1.004  -1.002  -0.960
197   D         0.128   0.139   0.100   0.073   0.085   0.026   0.009   0.027  -0.036
198   I        -1.146  -1.145  -1.122  -1.010  -1.007  -0.967  -1.004  -1.002  -0.960
199   R         0.128   0.109   0.089   0.197   0.173   0.101   0.009  -0.020  -0.054
200   T         0.128   0.109   0.089   0.168   0.129   0.058   0.009  -0.020  -0.054
201   Q        -1.146  -1.145  -1.122  -1.309  -1.313  -1.267  -1.004  -1.002  -0.960
202   Y         1.882   1.883   1.915   2.241   2.252   2.294   2.037   2.038   2.079
203   H         1.882   1.883   1.915   2.277   2.278   2.331   2.037   2.038   2.079
204   L         0.128   0.109   0.089  -0.123  -0.139  -0.154   0.009  -0.020  -0.054
205   R         0.128   0.139   0.097   0.220   0.240   0.177   0.009   0.027  -0.041
206   R         0.128   0.109   0.089   0.197   0.173   0.101   0.009  -0.020  -0.054
207   E         0.128   0.109   0.089  -0.049  -0.085  -0.114   0.009  -0.020  -0.054
208   K         1.882   1.883   1.915   1.687   1.694   1.739   2.037   2.038   2.079
209   L         1.882   1.883   1.915   1.510   1.518   1.562   2.037   2.038   2.079
210   A         0.128   0.109   0.089   0.352   0.316   0.278   0.009  -0.020  -0.054
211   T         0.128   0.109   0.089   0.442   0.410   0.375   0.009  -0.020  -0.054
212   W         0.128   0.109   0.089   0.307   0.267   0.194   0.009  -0.020  -0.054
213   D        -1.146  -1.145  -1.122  -1.267  -1.267  -1.225  -1.004  -1.002  -0.960
214   K        -1.146  -1.145  -1.122  -1.168  -1.157  -1.126  -1.004  -1.002  -0.960
215   Q         1.882   1.883   1.915   1.963   1.961   2.016   2.037   2.038   2.079
216   G        -1.146  -1.145  -1.122  -0.925  -0.929  -0.882  -1.004  -1.002  -0.960
217   N         0.128   0.139   0.100   0.290   0.302   0.252   0.009   0.027  -0.036
218   L         0.128   0.109   0.089  -0.180  -0.198  -0.218   0.009  -0.020  -0.054
219   K        -0.028  -0.028  -0.001  -0.267  -0.267  -0.222  -0.244  -0.243  -0.200
220   Y        -0.028  -0.028  -0.001  -0.267  -0.267  -0.222  -0.244  -0.243  -0.200
221   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/Q99176
Output conservation file - output/Q99176
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
