1     M         1.441   1.445   1.463   1.676   1.683   1.711   1.517   1.521   1.542
2     L        -1.187  -1.182  -1.158  -1.239  -1.212  -1.188  -1.062  -1.053  -1.013
3     L        -1.187  -1.182  -1.158  -1.232  -1.208  -1.181  -1.062  -1.053  -1.013
4     F        -1.187  -1.182  -1.158  -1.296  -1.293  -1.244  -1.062  -1.053  -1.013
5     P        -1.187  -1.182  -1.158  -1.225  -1.222  -1.174  -1.062  -1.053  -1.013
6     G        -1.187  -1.182  -1.158  -1.204  -1.200  -1.153  -1.062  -1.053  -1.013
7     L        -1.187  -1.182  -1.158  -1.239  -1.212  -1.188  -1.062  -1.053  -1.013
8     K        -1.187  -1.182  -1.158  -1.076  -1.063  -1.025  -1.062  -1.053  -1.013
9     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
10    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
11    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
12    P         1.441   1.445   1.463   1.483   1.489   1.518   1.517   1.521   1.542
13    V         1.441   1.445   1.463   1.496   1.503   1.532   1.517   1.521   1.542
14    L        -1.187  -1.182  -1.158  -1.143  -1.115  -1.092  -1.062  -1.053  -1.013
15    N        -0.081  -0.099  -0.131  -0.150  -0.186  -0.268  -0.202  -0.229  -0.278
16    A        -0.081  -0.099  -0.131   0.030  -0.002  -0.047  -0.202  -0.229  -0.278
17    S        -1.187  -1.182  -1.158  -1.170  -1.152  -1.119  -1.062  -1.053  -1.013
18    T        -1.187  -1.182  -1.158  -1.218  -1.217  -1.167  -1.062  -1.053  -1.013
19    V        -1.187  -1.182  -1.158  -1.346  -1.345  -1.294  -1.062  -1.053  -1.013
20    I        -0.081  -0.035  -0.111  -0.415  -0.343  -0.453  -0.202  -0.130  -0.246
21    V        -1.187  -1.182  -1.158  -0.939  -0.929  -0.890  -1.062  -1.053  -1.013
22    N        -0.081  -0.035  -0.111   0.059   0.132   0.013  -0.202  -0.130  -0.246
23    P        -1.187  -1.182  -1.158  -0.802  -0.783  -0.753  -1.062  -1.053  -1.013
24    V        -1.187  -1.182  -1.158  -1.368  -1.369  -1.316  -1.062  -1.053  -1.013
25    R        -0.081  -0.096  -0.129  -0.054  -0.087  -0.153  -0.202  -0.225  -0.274
26    A        -1.187  -1.182  -1.158  -0.965  -0.959  -0.915  -1.062  -1.053  -1.013
27    V        -0.081  -0.099  -0.131  -0.089  -0.120  -0.177  -0.202  -0.229  -0.278
28    -         1.441   1.445   1.463   1.542   1.549   1.577   1.517   1.521   1.542
29    F        -0.081  -0.099  -0.131  -0.265  -0.303  -0.377  -0.202  -0.229  -0.278
30    P        -0.081  -0.096  -0.129  -0.029  -0.052  -0.102  -0.202  -0.225  -0.274
31    G        -1.187  -1.182  -1.158  -1.191  -1.187  -1.139  -1.062  -1.053  -1.013
32    L        -0.081  -0.035  -0.111  -0.223  -0.133  -0.292  -0.202  -0.130  -0.246
33    V        -0.081  -0.099  -0.131  -0.084  -0.116  -0.171  -0.202  -0.229  -0.278
34    L        -0.081  -0.035  -0.111  -0.393  -0.305  -0.457  -0.202  -0.130  -0.246
35    S         1.441   1.445   1.463   1.304   1.312   1.341   1.517   1.521   1.542
36    T        -1.187  -1.182  -1.158  -1.184  -1.182  -1.133  -1.062  -1.053  -1.013
37    K         1.441   1.445   1.463   1.472   1.482   1.507   1.517   1.521   1.542
38    R        -0.217  -0.214  -0.191  -0.225  -0.221  -0.179  -0.417  -0.412  -0.374
39    S        -0.217  -0.214  -0.191  -0.443  -0.439  -0.396  -0.417  -0.412  -0.374
40    F        -0.217  -0.214  -0.191  -0.338  -0.334  -0.292  -0.417  -0.412  -0.374
41    Y         1.441   1.445   1.463   1.480   1.487   1.516   1.517   1.521   1.542
42    S        -0.217  -0.214  -0.191  -0.467  -0.463  -0.420  -0.417  -0.412  -0.374
43    I        -0.217  -0.214  -0.191  -0.625  -0.621  -0.577  -0.417  -0.412  -0.374
44    N        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
45    R        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
46    L        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
47    N        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
48    A        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
49    E        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
50    N        -0.217  -0.214  -0.191  -0.226  -0.221  -0.180  -0.417  -0.412  -0.374
51    K        -0.217  -0.214  -0.191  -0.261  -0.256  -0.215  -0.417  -0.412  -0.374
52    I        -0.217  -0.214  -0.191  -0.284  -0.280  -0.238  -0.417  -0.412  -0.374
53    N        -0.217  -0.214  -0.191  -0.203  -0.198  -0.157  -0.417  -0.412  -0.374
54    D        -0.217  -0.214  -0.191  -0.237  -0.233  -0.192  -0.417  -0.412  -0.374
55    I        -0.081  -0.096  -0.129  -0.110  -0.135  -0.185  -0.202  -0.225  -0.274
56    A        -1.187  -1.182  -1.158  -1.010  -1.003  -0.960  -1.062  -1.053  -1.013
57    N        -1.187  -1.182  -1.158  -1.062  -1.058  -1.012  -1.062  -1.053  -1.013
58    T        -1.187  -1.182  -1.158  -0.990  -0.983  -0.941  -1.062  -1.053  -1.013
59    S        -1.187  -1.182  -1.158  -1.177  -1.160  -1.126  -1.062  -1.053  -1.013
60    K        -0.081  -0.099  -0.131  -0.074  -0.102  -0.156  -0.202  -0.229  -0.278
61    E        -0.081  -0.099  -0.131   0.045   0.013  -0.044  -0.202  -0.229  -0.278
62    A        -1.187  -1.182  -1.158  -1.296  -1.299  -1.244  -1.062  -1.053  -1.013
63    S        -0.081  -0.099  -0.131  -0.223  -0.252  -0.295  -0.202  -0.229  -0.278
64    S        -1.187  -1.182  -1.158  -1.191  -1.175  -1.139  -1.062  -1.053  -1.013
65    S        -1.187  -1.182  -1.158  -1.102  -1.080  -1.052  -1.062  -1.053  -1.013
66    V        -0.081  -0.096  -0.129   0.025   0.003  -0.037  -0.202  -0.225  -0.274
67    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
68    -        -0.217  -0.214  -0.191  -0.238  -0.233  -0.192  -0.417  -0.412  -0.374
69    -        -0.217  -0.214  -0.191  -0.249  -0.245  -0.204  -0.417  -0.412  -0.374
70    Q        -0.081  -0.099  -0.131   0.184   0.156   0.095  -0.202  -0.229  -0.278
71    M        -0.081  -0.099  -0.131  -0.039  -0.079  -0.163  -0.202  -0.229  -0.278
72    F        -1.187  -1.182  -1.158  -1.156  -1.145  -1.105  -1.062  -1.053  -1.013
73    K         1.441   1.445   1.463   1.472   1.482   1.507   1.517   1.521   1.542
74    P        -0.217  -0.214  -0.191  -0.068  -0.063  -0.023  -0.417  -0.412  -0.374
75    P        -1.187  -1.182  -1.158  -1.036  -1.028  -0.986  -1.062  -1.053  -1.013
76    E        -0.081  -0.096  -0.129   0.151   0.129   0.086  -0.202  -0.225  -0.274
77    F        -0.081  -0.035  -0.111  -0.084  -0.007  -0.135  -0.202  -0.130  -0.246
78    S        -1.187  -1.182  -1.158  -1.170  -1.152  -1.119  -1.062  -1.053  -1.013
79    Q        -0.081  -0.099  -0.131   0.268   0.242   0.196  -0.202  -0.229  -0.278
80    F        -0.081  -0.096  -0.129  -0.105  -0.130  -0.181  -0.202  -0.225  -0.274
81    K        -1.187  -1.182  -1.158  -0.933  -0.918  -0.883  -1.062  -1.053  -1.013
82    D        -0.081  -0.099  -0.131   0.005  -0.027  -0.081  -0.202  -0.229  -0.278
83    S         1.441   1.445   1.463   1.304   1.312   1.341   1.517   1.521   1.542
84    Y        -0.081  -0.096  -0.129  -0.105  -0.128  -0.186  -0.202  -0.225  -0.274
85    Q        -0.081  -0.099  -0.131   0.236   0.207   0.157  -0.202  -0.229  -0.278
86    K        -1.187  -1.182  -1.158  -0.914  -0.895  -0.864  -1.062  -1.053  -1.013
87    D         1.441   1.445   1.463   1.559   1.565   1.594   1.517   1.521   1.542
88    Y         1.441   1.445   1.463   1.461   1.471   1.497   1.517   1.521   1.542
89    E        -0.081  -0.096  -0.129   0.132   0.107   0.063  -0.202  -0.225  -0.274
90    R        -0.081  -0.096  -0.129   0.020  -0.008  -0.064  -0.202  -0.225  -0.274
91    I        -0.081  -0.096  -0.129  -0.125  -0.148  -0.203  -0.202  -0.225  -0.274
92    A        -1.187  -1.182  -1.158  -1.109  -1.106  -1.058  -1.062  -1.053  -1.013
93    K        -1.187  -1.182  -1.158  -1.043  -1.029  -0.992  -1.062  -1.053  -1.013
94    Y        -0.081  -0.099  -0.131  -0.084  -0.112  -0.166  -0.202  -0.229  -0.278
95    T        -0.081  -0.099  -0.131  -0.135  -0.172  -0.234  -0.202  -0.229  -0.278
96    L         1.441   1.445   1.463   1.132   1.139   1.170   1.517   1.521   1.542
97    I        -1.187  -1.182  -1.158  -1.368  -1.364  -1.316  -1.062  -1.053  -1.013
98    P         1.441   1.445   1.463   1.483   1.489   1.518   1.517   1.521   1.542
99    L         1.441   1.445   1.463   1.132   1.139   1.170   1.517   1.521   1.542
100   T         1.441   1.445   1.463   1.493   1.498   1.529   1.517   1.521   1.542
101   M        -0.081  -0.096  -0.129   0.113   0.085   0.024  -0.202  -0.225  -0.274
102   V        -0.081  -0.096  -0.129  -0.064  -0.090  -0.143  -0.202  -0.225  -0.274
103   P        -0.081  -0.096  -0.129  -0.059  -0.086  -0.137  -0.202  -0.225  -0.274
104   F        -0.081  -0.099  -0.131  -0.265  -0.303  -0.377  -0.202  -0.229  -0.278
105   Y        -0.081  -0.096  -0.129  -0.084  -0.108  -0.162  -0.202  -0.225  -0.274
106   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
107   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
108   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
109   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
110   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
111   A        -0.081  -0.096  -0.129  -0.094  -0.125  -0.191  -0.202  -0.225  -0.274
112   S        -1.187  -1.182  -1.158  -1.218  -1.204  -1.167  -1.062  -1.053  -1.013
113   F        -0.081  -0.096  -0.129  -0.130  -0.156  -0.211  -0.202  -0.225  -0.274
114   T        -1.187  -1.182  -1.158  -1.089  -1.082  -1.038  -1.062  -1.053  -1.013
115   G        -1.187  -1.182  -1.158  -1.218  -1.217  -1.167  -1.062  -1.053  -1.013
116   G         1.441   1.445   1.463   1.483   1.488   1.518   1.517   1.521   1.542
117   V        -1.187  -1.182  -1.158  -1.069  -1.060  -1.019  -1.062  -1.053  -1.013
118   I        -1.187  -1.182  -1.158  -1.149  -1.138  -1.099  -1.062  -1.053  -1.013
119   N        -1.187  -1.182  -1.158  -0.978  -0.971  -0.928  -1.062  -1.053  -1.013
120   P         1.441   1.445   1.463   1.483   1.489   1.518   1.517   1.521   1.542
121   L        -1.187  -1.182  -1.158  -1.204  -1.177  -1.153  -1.062  -1.053  -1.013
122   L        -0.081  -0.099  -0.131  -0.415  -0.442  -0.484  -0.202  -0.229  -0.278
123   D         1.441   1.445   1.463   1.559   1.565   1.594   1.517   1.521   1.542
124   A        -1.187  -1.182  -1.158  -1.218  -1.219  -1.167  -1.062  -1.053  -1.013
125   S        -1.187  -1.182  -1.158  -1.442  -1.436  -1.390  -1.062  -1.053  -1.013
126   L        -0.081  -0.096  -0.129  -0.415  -0.438  -0.481  -0.202  -0.225  -0.274
127   S        -0.081  -0.099  -0.131  -0.202  -0.229  -0.270  -0.202  -0.229  -0.278
128   S         1.441   1.445   1.463   1.304   1.312   1.341   1.517   1.521   1.542
129   I        -1.187  -1.182  -1.158  -1.361  -1.358  -1.309  -1.062  -1.053  -1.013
130   F        -0.081  -0.099  -0.131  -0.130  -0.161  -0.215  -0.202  -0.229  -0.278
131   L         1.441   1.445   1.463   1.132   1.139   1.170   1.517   1.521   1.542
132   I        -0.081  -0.096  -0.129  -0.135  -0.160  -0.215  -0.202  -0.225  -0.274
133   Y        -1.187  -1.182  -1.158  -1.116  -1.106  -1.065  -1.062  -1.053  -1.013
134   L        -1.187  -1.182  -1.158  -1.289  -1.266  -1.237  -1.062  -1.053  -1.013
135   Q        -1.187  -1.182  -1.158  -0.870  -0.869  -0.821  -1.062  -1.053  -1.013
136   Y        -0.081  -0.099  -0.131  -0.244  -0.279  -0.357  -0.202  -0.229  -0.278
137   G        -0.081  -0.096  -0.129   0.025   0.001  -0.037  -0.202  -0.225  -0.274
138   F        -0.081  -0.099  -0.131  -0.130  -0.161  -0.215  -0.202  -0.229  -0.278
139   T         1.441   1.445   1.463   1.493   1.498   1.529   1.517   1.521   1.542
140   S         1.441   1.445   1.463   1.304   1.312   1.341   1.517   1.521   1.542
141   C        -0.081  -0.096  -0.129   0.050   0.016  -0.073  -0.202  -0.225  -0.274
142   I         1.441   1.445   1.463   1.427   1.436   1.464   1.517   1.521   1.542
143   I        -0.081  -0.096  -0.129  -0.275  -0.306  -0.382  -0.202  -0.225  -0.274
144   D         1.441   1.445   1.463   1.559   1.565   1.594   1.517   1.521   1.542
145   Y         1.441   1.445   1.463   1.461   1.471   1.497   1.517   1.521   1.542
146   I         1.441   1.445   1.463   1.427   1.436   1.464   1.517   1.521   1.542
147   P         1.441   1.445   1.463   1.483   1.489   1.518   1.517   1.521   1.542
148   K         1.441   1.445   1.463   1.472   1.482   1.507   1.517   1.521   1.542
149   E        -0.081  -0.099  -0.131   0.088   0.058   0.008  -0.202  -0.229  -0.278
150   K         1.441   1.445   1.463   1.472   1.482   1.507   1.517   1.521   1.542
151   Y        -0.081  -0.035  -0.111  -0.115  -0.038  -0.162  -0.202  -0.130  -0.246
152   P        -1.187  -1.182  -1.158  -1.122  -1.114  -1.072  -1.062  -1.053  -1.013
153   R        -0.081  -0.099  -0.131   0.005  -0.030  -0.086  -0.202  -0.229  -0.278
154   W        -0.081  -0.096  -0.129   0.030  -0.004  -0.091  -0.202  -0.225  -0.274
155   H         1.441   1.445   1.463   1.676   1.681   1.711   1.517   1.521   1.542
156   K         1.441   1.445   1.463   1.472   1.482   1.507   1.517   1.521   1.542
157   L        -0.081  -0.099  -0.131  -0.361  -0.387  -0.419  -0.202  -0.229  -0.278
158   A         1.441   1.445   1.463   1.537   1.542   1.573   1.517   1.521   1.542
159   L        -1.187  -1.182  -1.158  -1.375  -1.349  -1.323  -1.062  -1.053  -1.013
160   Y        -0.081  -0.099  -0.131  -0.105  -0.133  -0.190  -0.202  -0.229  -0.278
161   C        -1.187  -1.182  -1.158  -1.109  -1.121  -1.058  -1.062  -1.053  -1.013
162   L         1.441   1.445   1.463   1.132   1.139   1.170   1.517   1.521   1.542
163   Y        -0.081  -0.099  -0.131  -0.059  -0.086  -0.136  -0.202  -0.229  -0.278
164   G        -1.187  -1.182  -1.158  -1.450  -1.455  -1.397  -1.062  -1.053  -1.013
165   G        -0.081  -0.096  -0.129  -0.039  -0.066  -0.113  -0.202  -0.225  -0.274
166   S        -0.081  -0.096  -0.129  -0.223  -0.248  -0.291  -0.202  -0.225  -0.274
167   M        -1.187  -1.182  -1.158  -1.102  -1.108  -1.052  -1.062  -1.053  -1.013
168   L        -0.081  -0.035  -0.111  -0.265  -0.176  -0.332  -0.202  -0.130  -0.246
169   S        -0.081  -0.096  -0.129  -0.228  -0.252  -0.297  -0.202  -0.225  -0.274
170   L         1.441   1.445   1.463   1.132   1.139   1.170   1.517   1.521   1.542
171   Y        -0.081  -0.096  -0.129  -0.105  -0.128  -0.186  -0.202  -0.225  -0.274
172   G         1.441   1.445   1.463   1.483   1.488   1.518   1.517   1.521   1.542
173   I         1.441   1.445   1.463   1.427   1.436   1.464   1.517   1.521   1.542
174   Y         1.441   1.445   1.463   1.461   1.471   1.497   1.517   1.521   1.542
175   E        -0.081  -0.099  -0.131   0.045   0.013  -0.044  -0.202  -0.229  -0.278
176   L         1.441   1.445   1.463   1.132   1.139   1.170   1.517   1.521   1.542
177   E         1.441   1.445   1.463   1.602   1.609   1.637   1.517   1.521   1.542
178   T         1.441   1.445   1.463   1.493   1.498   1.529   1.517   1.521   1.542
179   K        -1.187  -1.182  -1.158  -1.156  -1.146  -1.105  -1.062  -1.053  -1.013
180   N         1.441   1.445   1.463   1.559   1.569   1.594   1.517   1.521   1.542
181   N         1.441   1.445   1.463   1.559   1.569   1.594   1.517   1.521   1.542
182   G         1.441   1.445   1.463   1.483   1.488   1.518   1.517   1.521   1.542
183   F        -1.187  -1.182  -1.158  -1.361  -1.359  -1.309  -1.062  -1.053  -1.013
184   V        -0.081  -0.099  -0.131  -0.089  -0.120  -0.177  -0.202  -0.229  -0.278
185   D         1.441   1.445   1.463   1.559   1.565   1.594   1.517   1.521   1.542
186   L         1.441   1.445   1.463   1.132   1.139   1.170   1.517   1.521   1.542
187   V         1.441   1.445   1.463   1.483   1.490   1.518   1.517   1.521   1.542
188   K        -1.187  -1.182  -1.158  -1.170  -1.164  -1.119  -1.062  -1.053  -1.013
189   K        -0.081  -0.099  -0.131  -0.069  -0.097  -0.150  -0.202  -0.229  -0.278
190   L         1.441   1.445   1.463   1.132   1.139   1.170   1.517   1.521   1.542
191   W         1.441   1.445   1.463   1.750   1.757   1.784   1.517   1.521   1.542
192   N        -1.187  -1.182  -1.158  -1.129  -1.125  -1.078  -1.062  -1.053  -1.013
193   E        -0.081  -0.099  -0.131   0.040   0.010  -0.050  -0.202  -0.229  -0.278
194   N        -0.081  -0.035  -0.111   0.040   0.114  -0.006  -0.202  -0.130  -0.246
195   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
196   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
197   D         1.441   1.445   1.463   1.559   1.565   1.594   1.517   1.521   1.542
198   D        -0.081  -0.035  -0.111  -0.192  -0.122  -0.228  -0.202  -0.130  -0.246
199   H        -1.187  -1.182  -1.158  -0.802  -0.799  -0.753  -1.062  -1.053  -1.013
200   L        -0.217  -0.214  -0.191  -0.563  -0.559  -0.516  -0.417  -0.412  -0.374
201   Y         1.441   1.445   1.463   1.480   1.487   1.516   1.517   1.521   1.542
202   I        -0.081  -0.099  -0.131  -0.115  -0.143  -0.194  -0.202  -0.229  -0.278
203   F         1.441   1.445   1.463   1.439   1.445   1.475   1.517   1.521   1.542
204   G        -0.081  -0.096  -0.129  -0.084  -0.112  -0.167  -0.202  -0.225  -0.274
205   R         1.441   1.445   1.463   1.580   1.584   1.616   1.517   1.521   1.542
206   N        -1.187  -1.182  -1.158  -1.102  -1.096  -1.052  -1.062  -1.053  -1.013
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/Q08749
Output conservation file - output/Q08749
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
