1     M         2.057   2.120   2.227   2.228   2.334   2.477   2.303   2.404   2.593
2     P         2.057   2.120   2.227   2.207   2.309   2.455   2.303   2.404   2.593
3     V        -0.135  -0.145  -0.052  -0.335  -0.348  -0.209  -0.427  -0.434  -0.313
4     T        -0.135  -0.145  -0.052  -0.157  -0.170  -0.029  -0.427  -0.434  -0.313
5     K        -0.044  -0.024   0.013  -0.351  -0.339  -0.272  -0.304  -0.263  -0.219
6     S        -0.044  -0.076   0.003  -0.039  -0.066   0.028  -0.304  -0.333  -0.235
7     L         1.121   1.105   1.039   1.044   1.027   0.941   1.118   1.080   0.924
8     S        -1.185  -1.174  -1.125  -1.064  -1.082  -1.000  -1.014  -0.992  -0.913
9     K         1.121   1.105   1.039   0.844   0.841   0.720   1.118   1.080   0.924
10    L        -0.524  -0.485  -0.555  -0.327  -0.271  -0.402  -0.540  -0.494  -0.566
11    Q        -0.705  -0.750  -0.651  -0.875  -0.979  -0.849  -0.777  -0.808  -0.683
12    K         1.121   1.105   1.039   0.850   0.843   0.729   1.118   1.080   0.924
13    N        -0.524  -0.567  -0.560  -0.381  -0.462  -0.479  -0.540  -0.594  -0.573
14    L         0.617   0.675   0.778   0.290   0.400   0.540   0.170   0.258   0.399
15    S        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
16    K        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
17    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
18    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
19    K        -0.044  -0.076   0.003  -0.375  -0.395  -0.282  -0.304  -0.333  -0.235
20    G         0.734   0.827   0.860   0.521   0.699   0.673   0.407   0.563   0.569
21    K        -0.044  -0.061   0.014  -0.261  -0.252  -0.162  -0.304  -0.310  -0.217
22    N        -1.185  -1.174  -1.125  -1.213  -1.153  -1.152  -1.014  -0.992  -0.913
23    I        -1.185  -1.174  -1.125  -1.115  -1.141  -1.062  -1.014  -0.992  -0.913
24    T        -0.705  -0.696  -0.629  -0.656  -0.634  -0.526  -0.777  -0.755  -0.659
25    V        -0.044   0.067   0.050  -0.189  -0.032  -0.035  -0.304  -0.138  -0.164
26    H         2.057   2.120   2.227   2.280   2.369   2.530   2.303   2.404   2.593
27    P        -0.524  -0.520  -0.566  -0.208  -0.209  -0.277  -0.540  -0.535  -0.580
28    K         1.121   1.105   1.039   0.900   0.902   0.814   1.118   1.080   0.924
29    G         0.254   0.119   0.106   0.491   0.288   0.257   0.170  -0.035  -0.071
30    R         2.057   2.120   2.227   1.921   1.984   2.165   2.303   2.404   2.593
31    K         1.121   1.026   1.018   0.850   0.729   0.697   1.118   0.938   0.884
32    Y         0.254   0.222   0.147   0.557   0.505   0.409   0.170   0.111  -0.008
33    E        -0.524  -0.485  -0.555  -0.845  -0.805  -0.913  -0.540  -0.494  -0.566
34    K         0.254   0.222   0.147   0.282   0.216   0.041   0.170   0.111  -0.008
35    L         1.121   1.105   1.039   1.031   1.013   0.921   1.118   1.080   0.924
36    V         0.254   0.222   0.147   0.528   0.509   0.383   0.170   0.111  -0.008
37    R         1.121   1.026   1.018   0.983   0.797   0.770   1.118   0.938   0.884
38    A         2.057   2.120   2.227   2.149   2.219   2.396   2.303   2.404   2.593
39    T         0.254   0.119   0.106   0.383   0.196   0.150   0.170  -0.035  -0.071
40    M         0.071   0.051   0.076  -0.004  -0.015   0.026  -0.134  -0.139  -0.125
41    R         2.057   2.120   2.227   1.934   1.985   2.178   2.303   2.404   2.593
42    E         0.638   0.665   0.772   0.274   0.218   0.470   0.214   0.237   0.387
43    D        -1.008  -0.836  -0.990  -0.913  -0.689  -0.924  -0.831  -0.633  -0.778
44    K         1.205   1.079   1.066   0.924   0.833   0.711   1.258   1.034   0.976
45    I        -0.185  -0.062  -0.033  -0.379  -0.217  -0.160  -0.482  -0.307  -0.283
46    A        -1.008  -1.078  -1.053  -0.764  -0.927  -0.846  -0.831  -0.897  -0.843
47    A        -1.163  -1.149  -1.099  -1.159  -1.152  -1.102  -1.005  -0.975  -0.892
48    K         0.403   0.303   0.196   0.216   0.183  -0.072   0.388   0.234   0.067
49    K         1.205   1.079   1.066   0.957   0.894   0.773   1.258   1.034   0.976
50    K        -1.574  -1.593  -1.515  -1.741  -1.811  -1.685  -1.179  -1.176  -1.070
51    L        -1.008  -0.973  -1.024  -0.931  -0.872  -1.031  -0.831  -0.781  -0.814
52    H        -0.596  -0.593  -0.604  -0.366  -0.433  -0.398  -0.656  -0.652  -0.635
53    Q        -1.163  -1.203  -1.100  -1.041  -1.105  -0.955  -1.005  -1.020  -0.893
54    D        -0.340  -0.428  -0.491  -0.384  -0.590  -0.643  -0.308  -0.423  -0.488
55    K        -0.596  -0.637  -0.608  -0.919  -0.913  -0.878  -0.656  -0.695  -0.638
56    R         0.071  -0.020   0.042  -0.233  -0.386  -0.306  -0.134  -0.251  -0.177
57    V        -0.340  -0.386  -0.473  -0.057  -0.137  -0.251  -0.308  -0.371  -0.465
58    H        -1.008  -1.078  -1.053  -0.661  -0.760  -0.723  -0.831  -0.897  -0.843
59    E         0.403   0.226   0.168   0.403   0.088   0.072   0.388   0.124   0.024
60    L         0.403   0.226   0.168   0.433   0.184   0.111   0.388   0.124   0.024
61    A         0.071   0.045   0.030   0.147   0.093   0.064  -0.134  -0.199  -0.204
62    R         0.403   0.445   0.224   0.479   0.511   0.247   0.388   0.452   0.109
63    V         0.071   0.133   0.062   0.211   0.272   0.199  -0.134  -0.089  -0.157
64    K        -1.574  -1.581  -1.513  -1.480  -1.361  -1.318  -1.179  -1.168  -1.068
65    F         2.057   2.120   2.227   2.167   2.246   2.415   2.303   2.404   2.593
66    M        -0.751  -0.647  -0.638  -0.571  -0.430  -0.393  -0.831  -0.715  -0.673
67    Q         0.403   0.364   0.218   0.552   0.518   0.245   0.388   0.332   0.101
68    D        -0.185  -0.154  -0.070  -0.390  -0.467  -0.249  -0.482  -0.444  -0.335
69    V        -1.163  -1.153  -1.091  -1.016  -0.996  -0.921  -1.005  -0.974  -0.885
70    V        -1.163  -1.203  -1.100  -0.963  -1.002  -0.872  -1.005  -1.020  -0.893
71    N        -1.008  -1.029  -1.045  -1.062  -1.037  -1.068  -0.831  -0.847  -0.835
72    S        -0.705  -0.623  -0.622  -0.937  -0.859  -0.845  -0.777  -0.676  -0.652
73    D        -0.524  -0.649  -0.617  -0.455  -0.674  -0.664  -0.540  -0.687  -0.642
74    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
75    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
76    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
77    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
78    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
79    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
80    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
81    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
82    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
83    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
84    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
85    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
86    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
87    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
88    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
89    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
90    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
91    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
92    T        -0.135  -0.072  -0.051  -0.329  -0.281  -0.208  -0.427  -0.339  -0.311
93    F         0.083   0.115   0.055   0.204   0.232   0.137  -0.086  -0.046  -0.149
94    K        -0.524  -0.611  -0.617  -0.771  -0.802  -0.871  -0.540  -0.641  -0.642
95    G        -0.596  -0.477  -0.547  -0.649  -0.559  -0.552  -0.656  -0.507  -0.570
96    Q         0.071   0.003   0.058  -0.250  -0.231  -0.247  -0.134  -0.215  -0.159
97    P        -0.596  -0.531  -0.543  -0.993  -0.857  -0.904  -0.656  -0.567  -0.565
98    I        -1.574  -1.554  -1.488  -1.148  -1.083  -1.012  -1.179  -1.147  -1.049
99    F         0.403   0.226   0.168   0.697   0.421   0.362   0.388   0.124   0.024
100   D        -1.163  -1.149  -1.099  -1.129  -1.203  -1.050  -1.005  -0.975  -0.892
101   H        -0.596  -0.491  -0.563  -0.465  -0.353  -0.381  -0.656  -0.510  -0.582
102   A        -1.008  -0.946  -1.005  -1.034  -0.979  -1.041  -0.831  -0.763  -0.794
103   H        -0.340  -0.386  -0.473  -0.232  -0.356  -0.388  -0.308  -0.371  -0.465
104   T        -0.751  -0.649  -0.642  -0.618  -0.451  -0.441  -0.831  -0.715  -0.675
105   R        -0.596  -0.556  -0.580  -0.852  -0.718  -0.818  -0.656  -0.591  -0.603
106   E        -1.574  -1.593  -1.515  -1.451  -1.536  -1.406  -1.179  -1.176  -1.070
107   F        -0.596  -0.601  -0.576  -0.575  -0.614  -0.591  -0.656  -0.663  -0.604
108   I         1.205   1.183   1.085   1.400   1.385   1.238   1.258   1.215   1.012
109   Q         0.071   0.059   0.094  -0.080  -0.129  -0.030  -0.134  -0.126  -0.097
110   S        -1.574  -1.502  -1.479  -1.566  -1.499  -1.421  -1.179  -1.099  -1.041
111   F         0.814   0.817   0.846   0.922   0.903   0.955   0.562   0.542   0.540
112   I         0.403   0.226   0.168   0.688   0.456   0.357   0.388   0.124   0.024
113   E        -1.574  -1.452  -1.478  -1.145  -0.913  -1.007  -1.179  -1.056  -1.041
114   R         0.814   0.780   0.828   0.616   0.506   0.599   0.562   0.470   0.504
115   D         0.071  -0.020   0.042   0.159   0.025   0.140  -0.134  -0.251  -0.177
116   D         2.057   2.120   2.227   2.096   2.125   2.343   2.303   2.404   2.593
117   T        -1.163  -0.998  -1.071  -1.085  -0.970  -0.936  -1.005  -0.843  -0.868
118   E         2.057   2.120   2.227   1.885   1.913   2.128   2.303   2.404   2.593
119   L         1.205   1.079   1.066   1.189   1.027   1.036   1.258   1.034   0.976
120   D        -1.986  -1.930  -1.860  -1.752  -1.598  -1.562  -1.353  -1.292  -1.191
121   E        -0.596  -0.580  -0.572  -0.712  -0.746  -0.664  -0.656  -0.620  -0.600
122   L         0.403   0.375   0.196   0.459   0.411   0.197   0.388   0.332   0.067
123   K         1.205   1.274   1.101   0.954   1.146   0.821   1.258   1.367   1.042
124   K        -0.596  -0.644  -0.578  -0.874  -0.902  -0.827  -0.656  -0.690  -0.605
125   K        -0.185  -0.177  -0.073  -0.685  -0.590  -0.476  -0.482  -0.474  -0.340
126   R         1.205   1.357   1.098   1.184   1.343   1.044   1.258   1.500   1.035
127   R         2.057   2.120   2.227   1.934   1.985   2.178   2.303   2.404   2.593
128   S        -0.751  -0.700  -0.639  -0.768  -0.649  -0.618  -0.831  -0.764  -0.674
129   N         0.638   0.756   0.826   0.536   0.792   0.827   0.214   0.421   0.500
130   R         2.057   2.120   2.227   1.934   1.985   2.178   2.303   2.404   2.593
131   P         2.057   2.120   2.227   2.213   2.322   2.462   2.303   2.404   2.593
132   P         0.071   0.154   0.069   0.224   0.344   0.211  -0.134  -0.042  -0.144
133   S        -0.029   0.081   0.039  -0.053   0.113   0.076  -0.308  -0.173  -0.198
134   N        -0.596  -0.606  -0.578  -0.431  -0.404  -0.389  -0.656  -0.669  -0.606
135   R         1.205   1.183   1.085   1.118   1.064   0.904   1.258   1.215   1.012
136   Q         1.205   1.079   1.066   1.315   1.114   1.093   1.258   1.034   0.976
137   V        -1.163  -1.025  -1.071  -1.104  -0.958  -0.951  -1.005  -0.862  -0.868
138   L        -0.340  -0.362  -0.492  -0.185  -0.207  -0.352  -0.308  -0.330  -0.489
139   L         2.057   2.120   2.227   1.899   1.976   2.143   2.303   2.404   2.593
140   Q        -0.029  -0.090   0.019  -0.280  -0.436  -0.211  -0.308  -0.367  -0.223
141   Q        -1.163  -1.059  -1.086  -0.889  -0.724  -0.756  -1.005  -0.888  -0.880
142   R         0.071   0.099   0.073  -0.326  -0.213  -0.319  -0.134  -0.093  -0.138
143   R        -1.574  -1.588  -1.517  -1.568  -1.509  -1.482  -1.179  -1.173  -1.072
144   D        -0.596  -0.648  -0.585  -0.760  -0.862  -0.771  -0.656  -0.696  -0.613
145   Q        -1.574  -1.593  -1.515  -1.468  -1.491  -1.354  -1.179  -1.176  -1.070
146   E         0.403   0.303   0.196   0.362   0.123   0.043   0.388   0.234   0.067
147   L        -1.163  -1.215  -1.097  -1.154  -1.191  -1.085  -1.005  -1.030  -0.890
148   K        -0.596  -0.634  -0.585  -0.849  -0.898  -0.820  -0.656  -0.690  -0.614
149   E         0.403   0.445   0.224   0.428   0.433   0.195   0.388   0.452   0.109
150   F        -0.029  -0.078   0.008   0.075  -0.026   0.133  -0.308  -0.346  -0.233
151   K         0.071   0.041   0.032  -0.339  -0.338  -0.395  -0.134  -0.193  -0.199
152   A        -1.008  -0.893  -1.036  -0.837  -0.651  -0.911  -0.831  -0.707  -0.826
153   G         2.057   2.120   2.227   2.176   2.304   2.424   2.303   2.404   2.593
154   F         1.205   1.183   1.085   1.453   1.425   1.323   1.258   1.215   1.012
155   L         0.071  -0.020   0.042  -0.207  -0.290  -0.242  -0.134  -0.251  -0.177
156   C        -0.596  -0.698  -0.625  -0.273  -0.460  -0.353  -0.656  -0.754  -0.655
157   P         2.057   2.120   2.227   2.213   2.322   2.462   2.303   2.404   2.593
158   D         1.205   1.183   1.085   1.378   1.306   1.243   1.258   1.215   1.012
159   L         2.057   2.120   2.227   1.899   1.976   2.143   2.303   2.404   2.593
160   S        -0.596  -0.530  -0.553  -0.465  -0.335  -0.382  -0.656  -0.570  -0.576
161   D         0.814   0.768   0.821   0.783   0.654   0.748   0.562   0.445   0.490
162   A        -1.008  -0.847  -0.980  -0.999  -0.812  -0.961  -0.831  -0.644  -0.768
163   K        -0.596  -0.514  -0.557  -0.974  -0.895  -0.935  -0.656  -0.568  -0.583
164   N         0.071   0.283   0.109   0.115   0.464   0.183  -0.134   0.180  -0.074
165   M         0.403   0.362   0.218   0.701   0.657   0.449   0.388   0.314   0.101
166   E        -0.340  -0.190  -0.439  -0.306  -0.186  -0.445  -0.308  -0.117  -0.422
167   F        -0.596  -0.698  -0.625  -0.487  -0.644  -0.512  -0.656  -0.754  -0.655
168   L         0.403   0.445   0.224   0.442   0.495   0.209   0.388   0.452   0.109
169   R        -0.029  -0.078   0.008  -0.503  -0.537  -0.433  -0.308  -0.346  -0.233
170   N        -1.574  -1.581  -1.513  -1.399  -1.451  -1.332  -1.179  -1.168  -1.068
171   W         1.205   1.183   1.085   1.651   1.628   1.517   1.258   1.215   1.012
172   N         0.403   0.226   0.168   0.575   0.355   0.170   0.388   0.124   0.024
173   G         1.205   1.377   1.119   1.425   1.685   1.316   1.258   1.531   1.075
174   T         1.205   1.079   1.066   1.347   1.212   1.161   1.258   1.034   0.976
175   F        -0.524  -0.624  -0.617  -0.220  -0.354  -0.344  -0.540  -0.661  -0.642
176   G         1.205   1.079   1.066   1.433   1.309   1.248   1.258   1.034   0.976
177   L        -1.163  -1.182  -1.087  -0.937  -0.913  -0.822  -1.005  -1.005  -0.882
178   L        -0.185  -0.144  -0.057  -0.376  -0.313  -0.178  -0.482  -0.426  -0.317
179   N        -0.029   0.073   0.027  -0.072   0.092   0.031  -0.308  -0.181  -0.213
180   T         0.071   0.021   0.029   0.125   0.073   0.060  -0.134  -0.227  -0.205
181   L        -0.596  -0.546  -0.577  -0.561  -0.495  -0.501  -0.656  -0.594  -0.601
182   R        -0.340  -0.428  -0.491  -0.501  -0.529  -0.714  -0.308  -0.423  -0.488
183   L        -0.596  -0.551  -0.580  -0.444  -0.344  -0.362  -0.656  -0.587  -0.602
184   I         1.205   1.079   1.066   1.412   1.257   1.230   1.258   1.034   0.976
185   R         0.403   0.274   0.196   0.414   0.195   0.095   0.388   0.193   0.066
186   I         0.814   0.862   0.892   0.847   0.923   0.941   0.562   0.630   0.635
187   N        -0.596  -0.698  -0.625  -0.509  -0.638  -0.567  -0.656  -0.754  -0.655
188   D        -1.163  -1.171  -1.119  -1.196  -1.154  -1.083  -1.005  -0.995  -0.910
189   K        -0.596  -0.551  -0.580  -0.779  -0.676  -0.753  -0.656  -0.587  -0.602
190   G         0.071   0.054   0.057   0.232   0.256   0.214  -0.134  -0.152  -0.155
191   E        -0.596  -0.637  -0.582  -0.967  -1.048  -0.969  -0.656  -0.687  -0.610
192   Q        -1.163  -1.164  -1.116  -1.265  -1.255  -1.207  -1.005  -0.990  -0.907
193   V        -0.596  -0.698  -0.625  -0.480  -0.614  -0.493  -0.656  -0.754  -0.655
194   V        -0.044   0.129   0.027   0.006   0.300   0.096  -0.304  -0.057  -0.198
195   G        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
196   G        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
197   N        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
198   E        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
199   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
200   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
201   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
202   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
203   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
204   -        -0.823  -0.788  -0.685  -0.774  -0.688  -0.569  -0.896  -0.847  -0.718
205   -        -1.185  -1.203  -1.129  -1.314  -1.324  -1.221  -1.014  -1.018  -0.918
206   -        -1.185  -1.113  -1.096  -1.261  -1.215  -1.121  -1.014  -0.932  -0.886
207   -        -1.185  -1.191  -1.106  -1.138  -1.121  -1.044  -1.014  -1.007  -0.895
208   -        -0.524  -0.529  -0.534  -0.517  -0.614  -0.509  -0.540  -0.546  -0.540
209   -        -0.524  -0.611  -0.573  -0.356  -0.466  -0.463  -0.540  -0.642  -0.589
210   -        -0.524  -0.611  -0.573  -0.704  -0.891  -0.814  -0.540  -0.642  -0.589
211   -         0.254   0.171   0.142   0.523   0.389   0.337   0.170   0.041  -0.015
212   -        -0.524  -0.425  -0.505  -0.468  -0.263  -0.481  -0.540  -0.410  -0.505
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/Q08687
Output conservation file - output/Q08687
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
