1     M         1.704   1.705   1.756   1.907   1.910   1.981   1.844   1.845   1.917
2     S        -0.269  -0.290  -0.253  -0.527  -0.552  -0.496  -0.459  -0.481  -0.425
3     D        -0.788  -0.814  -0.795  -0.571  -0.609  -0.576  -0.747  -0.775  -0.737
4     I         0.768   0.715   0.618   0.765   0.696   0.547   0.693   0.602   0.411
5     N        -0.269  -0.288  -0.257  -0.199  -0.221  -0.172  -0.459  -0.478  -0.430
6     E        -0.788  -0.823  -0.795  -0.748  -0.829  -0.803  -0.747  -0.785  -0.737
7     I        -1.306  -1.295  -1.233  -1.306  -1.266  -1.189  -1.035  -1.023  -0.940
8     E        -0.269  -0.288  -0.257  -0.499  -0.507  -0.448  -0.459  -0.478  -0.430
9     I        -0.788  -0.823  -0.795  -0.880  -0.943  -0.890  -0.747  -0.785  -0.737
10    P        -0.073  -0.131  -0.154   0.097   0.015  -0.017  -0.171  -0.252  -0.286
11    S        -0.788  -0.793  -0.758  -1.006  -1.031  -0.945  -0.747  -0.752  -0.699
12    R         0.445   0.439   0.484   0.119   0.108   0.195   0.117   0.104   0.160
13    K        -0.788  -0.771  -0.771  -0.533  -0.516  -0.527  -0.747  -0.728  -0.712
14    D        -0.269  -0.264  -0.247  -0.483  -0.453  -0.410  -0.459  -0.450  -0.418
15    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
16    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
17    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
18    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
19    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
20    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
21    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
22    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
23    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
24    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
25    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
26    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
27    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
28    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
29    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
30    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
31    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
32    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
33    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
34    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
35    E         0.445   0.481   0.495   0.400   0.462   0.476   0.117   0.183   0.182
36    I        -0.788  -0.799  -0.758  -0.564  -0.573  -0.509  -0.747  -0.760  -0.698
37    R        -0.788  -0.778  -0.773  -0.832  -0.811  -0.837  -0.747  -0.736  -0.714
38    Q        -1.306  -1.295  -1.233  -1.197  -1.190  -1.082  -1.035  -1.023  -0.940
39    V        -0.788  -0.778  -0.773  -0.853  -0.825  -0.826  -0.747  -0.736  -0.714
40    T        -0.269  -0.243  -0.248  -0.362  -0.325  -0.306  -0.459  -0.429  -0.419
41    P         0.768   0.716   0.655   0.885   0.803   0.671   0.693   0.603   0.478
42    K        -0.788  -0.793  -0.756  -0.864  -0.903  -0.830  -0.747  -0.753  -0.696
43    -        -0.241  -0.303  -0.257  -0.266  -0.384  -0.324  -0.405  -0.492  -0.428
44    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
45    -        -0.269  -0.288  -0.253  -0.127  -0.150  -0.101  -0.459  -0.478  -0.425
46    -        -0.788  -0.819  -0.795  -0.885  -0.923  -0.852  -0.747  -0.781  -0.737
47    -        -0.788  -0.797  -0.771  -0.771  -0.800  -0.755  -0.747  -0.758  -0.712
48    -        -0.073  -0.090  -0.154   0.136   0.116   0.004  -0.171  -0.195  -0.286
49    -        -0.269  -0.288  -0.253  -0.057  -0.084  -0.038  -0.459  -0.478  -0.425
50    -        -0.073  -0.090  -0.154   0.035   0.010  -0.101  -0.171  -0.195  -0.286
51    -         1.704   1.705   1.756   1.794   1.795   1.870   1.844   1.845   1.917
52    -        -0.788  -0.788  -0.767  -0.728  -0.724  -0.702  -0.747  -0.747  -0.708
53    -        -0.788  -0.819  -0.795  -0.984  -1.023  -0.967  -0.747  -0.781  -0.737
54    -        -0.073  -0.129  -0.185  -0.054  -0.147  -0.265  -0.171  -0.249  -0.329
55    -        -0.788  -0.738  -0.782  -0.666  -0.588  -0.655  -0.747  -0.690  -0.723
56    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
57    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
58    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
59    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
60    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
61    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
62    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
63    -        -0.241  -0.279  -0.257  -0.102  -0.154  -0.134  -0.405  -0.458  -0.428
64    -        -1.306  -1.295  -1.233  -1.065  -1.071  -0.952  -1.035  -1.023  -0.940
65    -        -0.788  -0.819  -0.795  -0.499  -0.547  -0.536  -0.747  -0.781  -0.737
66    -        -0.788  -0.797  -0.771  -0.891  -0.902  -0.838  -0.747  -0.758  -0.712
67    -        -0.788  -0.797  -0.771  -0.832  -0.857  -0.803  -0.747  -0.758  -0.712
68    -        -0.788  -0.819  -0.795  -0.632  -0.672  -0.635  -0.747  -0.781  -0.737
69    D        -0.783  -0.771  -0.776  -0.660  -0.644  -0.639  -0.755  -0.742  -0.720
70    P        -0.783  -0.763  -0.777  -0.646  -0.616  -0.637  -0.755  -0.735  -0.722
71    M        -0.783  -0.788  -0.785  -0.567  -0.575  -0.594  -0.755  -0.758  -0.730
72    H        -0.620  -0.635  -0.716  -0.313  -0.336  -0.449  -0.555  -0.572  -0.652
73    E        -1.215  -1.197  -1.184  -0.873  -0.849  -0.816  -0.955  -0.936  -0.898
74    I        -1.215  -1.216  -1.179  -1.167  -1.163  -1.085  -0.955  -0.953  -0.894
75    E        -1.215  -1.207  -1.193  -1.172  -1.160  -1.102  -0.955  -0.945  -0.906
76    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
77    D        -0.889  -0.882  -0.819  -0.932  -0.921  -0.819  -0.855  -0.847  -0.762
78    K        -0.889  -0.882  -0.819  -1.183  -1.186  -1.067  -0.855  -0.847  -0.762
79    S        -0.241  -0.214  -0.248  -0.461  -0.428  -0.442  -0.405  -0.368  -0.415
80    T        -0.889  -0.882  -0.819  -1.013  -1.009  -0.900  -0.855  -0.847  -0.762
81    Y        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
82    H        -0.889  -0.882  -0.819  -0.568  -0.559  -0.461  -0.855  -0.847  -0.762
83    A        -0.889  -0.882  -0.819  -1.062  -1.048  -0.948  -0.855  -0.847  -0.762
84    K        -0.241  -0.256  -0.257  -0.437  -0.475  -0.537  -0.405  -0.426  -0.428
85    I         1.704   1.705   1.756   1.790   1.792   1.866   1.844   1.845   1.917
86    K        -0.889  -0.882  -0.819  -1.126  -1.128  -1.011  -0.855  -0.847  -0.762
87    K        -0.241  -0.256  -0.257  -0.207  -0.243  -0.285  -0.405  -0.426  -0.428
88    S         1.704   1.705   1.756   1.435   1.436   1.516   1.844   1.845   1.917
89    D         0.652   0.649   0.595   0.666   0.659   0.532   0.495   0.487   0.370
90    S        -0.241  -0.247  -0.217  -0.298  -0.317  -0.276  -0.405  -0.413  -0.373
91    G        -0.889  -0.882  -0.819  -1.013  -0.998  -0.900  -0.855  -0.847  -0.762
92    T        -0.241  -0.256  -0.257  -0.354  -0.387  -0.440  -0.405  -0.426  -0.428
93    V        -0.241  -0.256  -0.257  -0.305  -0.322  -0.313  -0.405  -0.426  -0.428
94    L        -0.241  -0.256  -0.257  -0.210  -0.233  -0.264  -0.405  -0.426  -0.428
95    G        -0.788  -0.751  -0.795  -0.838  -0.769  -0.897  -0.747  -0.705  -0.737
96    A        -1.306  -1.297  -1.233  -1.198  -1.172  -1.082  -1.035  -1.025  -0.940
97    I        -0.269  -0.258  -0.261  -0.296  -0.270  -0.263  -0.459  -0.445  -0.435
98    P        -0.241  -0.219  -0.218  -0.376  -0.328  -0.376  -0.405  -0.375  -0.374
99    L        -0.889  -0.875  -0.819  -0.771  -0.751  -0.661  -0.855  -0.840  -0.762
100   N        -0.788  -0.754  -0.782  -0.815  -0.755  -0.805  -0.747  -0.708  -0.723
101   S        -0.788  -0.795  -0.758  -0.912  -0.918  -0.849  -0.747  -0.755  -0.699
102   R        -1.306  -1.297  -1.233  -0.966  -0.950  -0.853  -1.035  -1.025  -0.940
103   S        -0.889  -0.882  -0.819  -1.183  -1.187  -1.067  -0.855  -0.847  -0.762
104   S         1.704   1.705   1.756   1.435   1.436   1.516   1.844   1.845   1.917
105   S        -0.241  -0.237  -0.215  -0.279  -0.278  -0.256  -0.405  -0.400  -0.370
106   N        -0.241  -0.256  -0.257  -0.203  -0.225  -0.226  -0.405  -0.426  -0.428
107   S        -0.269  -0.243  -0.248  -0.734  -0.706  -0.670  -0.459  -0.429  -0.419
108   S        -0.073  -0.080  -0.147  -0.281  -0.303  -0.376  -0.171  -0.182  -0.275
109   V        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
110   T        -0.788  -0.799  -0.758  -0.941  -0.956  -0.874  -0.747  -0.760  -0.698
111   S        -0.269  -0.236  -0.247  -0.543  -0.498  -0.492  -0.459  -0.421  -0.418
112   T        -0.073  -0.080  -0.147  -0.183  -0.182  -0.315  -0.171  -0.182  -0.275
113   G        -0.788  -0.743  -0.763  -0.927  -0.866  -0.865  -0.747  -0.697  -0.704
114   Q        -0.788  -0.799  -0.758  -0.700  -0.704  -0.640  -0.747  -0.760  -0.698
115   S        -0.788  -0.823  -0.795  -1.030  -1.091  -1.068  -0.747  -0.785  -0.737
116   S        -0.783  -0.763  -0.770  -0.870  -0.841  -0.809  -0.755  -0.735  -0.715
117   R        -0.783  -0.780  -0.765  -0.613  -0.605  -0.587  -0.755  -0.750  -0.709
118   R        -1.647  -1.640  -1.572  -1.626  -1.620  -1.505  -1.155  -1.147  -1.059
119   V        -1.647  -1.640  -1.572  -1.626  -1.605  -1.505  -1.155  -1.147  -1.059
120   T        -1.215  -1.207  -1.193  -1.223  -1.210  -1.215  -0.955  -0.945  -0.906
121   K        -0.351  -0.346  -0.278  -0.790  -0.786  -0.678  -0.555  -0.548  -0.456
122   K        -1.647  -1.640  -1.572  -1.443  -1.430  -1.324  -1.155  -1.147  -1.059
123   T        -1.647  -1.640  -1.572  -1.292  -1.277  -1.175  -1.155  -1.147  -1.059
124   T         0.861   0.861   0.676   0.670   0.671   0.402   0.844   0.842   0.516
125   K         0.861   0.840   0.661   0.670   0.643   0.376   0.844   0.810   0.488
126   K         0.513   0.514   0.496   0.132   0.135   0.097   0.245   0.245   0.184
127   K         0.861   0.878   0.645   0.670   0.694   0.351   0.844   0.870   0.460
128   K         1.704   1.705   1.756   1.446   1.449   1.526   1.844   1.845   1.917
129   K         1.704   1.705   1.756   1.446   1.449   1.526   1.844   1.845   1.917
130   N        -0.620  -0.620  -0.686  -0.558  -0.559  -0.701  -0.555  -0.554  -0.619
131   A        -0.620  -0.621  -0.727  -0.382  -0.384  -0.580  -0.555  -0.555  -0.665
132   C         1.704   1.705   1.756   1.804   1.806   1.879   1.844   1.845   1.917
133   Y        -0.783  -0.770  -0.772  -0.704  -0.686  -0.680  -0.755  -0.741  -0.717
134   F         1.704   1.705   1.756   1.881   1.883   1.956   1.844   1.845   1.917
135   D        -1.215  -1.206  -1.205  -1.014  -0.998  -1.006  -0.955  -0.945  -0.916
136   T        -0.783  -0.789  -0.783  -0.890  -0.897  -0.872  -0.755  -0.758  -0.727
137   C         1.704   1.705   1.756   1.804   1.806   1.879   1.844   1.845   1.917
138   S         0.081   0.099  -0.129  -0.041  -0.016  -0.340   0.045   0.069  -0.251
139   S         0.861   0.878   0.645   0.703   0.725   0.402   0.844   0.870   0.460
140   A         0.081   0.081  -0.093   0.201   0.202  -0.084   0.045   0.044  -0.201
141   A         0.407   0.411   0.471   0.338   0.346   0.442   0.045   0.050   0.136
142   S        -0.188  -0.186  -0.220  -0.307  -0.307  -0.352  -0.355  -0.353  -0.384
143   K         0.407   0.411   0.471   0.124   0.129   0.243   0.045   0.050   0.136
144   F         0.407   0.411   0.471   0.478   0.484   0.577   0.045   0.050   0.136
145   I         0.513   0.514   0.539   0.524   0.526   0.558   0.245   0.245   0.266
146   G         1.704   1.705   1.756   1.798   1.800   1.874   1.844   1.845   1.917
147   D         0.081   0.114  -0.158   0.281   0.328  -0.098   0.045   0.089  -0.292
148   C         1.704   1.705   1.756   1.804   1.806   1.879   1.844   1.845   1.917
149   N        -0.188  -0.173  -0.235  -0.340  -0.320  -0.450  -0.355  -0.338  -0.404
150   F         0.513   0.531   0.503   0.636   0.665   0.605   0.245   0.276   0.198
151   C         1.704   1.705   1.756   1.804   1.806   1.879   1.844   1.845   1.917
152   K        -0.351  -0.346  -0.283  -0.515  -0.509  -0.409  -0.555  -0.548  -0.462
153   G         1.704   1.705   1.756   1.798   1.800   1.874   1.844   1.845   1.917
154   H         0.407   0.411   0.471   0.136   0.144   0.223   0.045   0.050   0.136
155   F         0.861   0.861   0.651   1.167   1.167   0.889   0.844   0.843   0.471
156   C         1.704   1.705   1.756   1.804   1.806   1.879   1.844   1.845   1.917
157   S         0.513   0.531   0.503   0.193   0.221   0.181   0.245   0.276   0.198
158   K         0.081   0.062  -0.120  -0.031  -0.056  -0.304   0.045   0.018  -0.238
159   H         1.704   1.705   1.756   1.861   1.863   1.936   1.844   1.845   1.917
160   R         1.704   1.705   1.756   1.845   1.847   1.920   1.844   1.845   1.917
161   L         1.704   1.705   1.756   1.756   1.759   1.833   1.844   1.845   1.917
162   M         0.513   0.514   0.510   0.545   0.547   0.539   0.245   0.244   0.211
163   E         1.704   1.705   1.756   1.746   1.748   1.822   1.844   1.845   1.917
164   N        -0.188  -0.191  -0.238  -0.105  -0.104  -0.160  -0.355  -0.355  -0.406
165   H         1.704   1.705   1.756   1.861   1.863   1.936   1.844   1.845   1.917
166   A        -1.215  -1.206  -1.205  -0.879  -0.866  -0.845  -0.955  -0.945  -0.916
167   C         1.704   1.705   1.756   1.804   1.806   1.879   1.844   1.845   1.917
168   N        -1.215  -1.214  -1.187  -1.061  -1.064  -1.035  -0.955  -0.952  -0.901
169   G         1.704   1.705   1.756   1.798   1.800   1.874   1.844   1.845   1.917
170   L         1.704   1.705   1.756   1.756   1.759   1.833   1.844   1.845   1.917
171   T        -0.188  -0.186  -0.220  -0.237  -0.234  -0.320  -0.355  -0.353  -0.384
172   S         0.081   0.114  -0.158   0.074   0.114  -0.193   0.045   0.089  -0.292
173   C         1.704   1.705   1.756   1.804   1.806   1.879   1.844   1.845   1.917
174   K         1.704   1.705   1.756   1.446   1.449   1.526   1.844   1.845   1.917
175   E         0.513   0.514   0.510   0.495   0.495   0.472   0.245   0.244   0.211
176   Q         0.513   0.531   0.503   0.547   0.578   0.510   0.245   0.276   0.198
177   L        -0.351  -0.346  -0.281  -0.308  -0.298  -0.198  -0.555  -0.548  -0.460
178   H         1.704   1.705   1.756   1.861   1.863   1.936   1.844   1.845   1.917
179   Q         0.407   0.411   0.471   0.327   0.332   0.424   0.045   0.050   0.136
180   R        -0.188  -0.191  -0.238  -0.222  -0.215  -0.298  -0.355  -0.355  -0.406
181   N         1.704   1.705   1.756   1.767   1.770   1.843   1.844   1.845   1.917
182   A         1.704   1.705   1.756   1.741   1.744   1.817   1.844   1.845   1.917
183   D        -0.188  -0.191  -0.238  -0.123  -0.129  -0.225  -0.355  -0.355  -0.406
184   K         1.704   1.705   1.756   1.446   1.449   1.526   1.844   1.845   1.917
185   L         1.704   1.705   1.756   1.756   1.759   1.833   1.844   1.845   1.917
186   E        -0.783  -0.780  -0.781  -0.547  -0.541  -0.538  -0.755  -0.750  -0.725
187   A        -1.215  -1.216  -1.179  -1.295  -1.292  -1.222  -0.955  -0.953  -0.894
188   E         1.704   1.705   1.756   1.746   1.748   1.822   1.844   1.845   1.917
189   Q         0.081   0.114  -0.158   0.301   0.348  -0.100   0.045   0.089  -0.292
190   T         0.861   0.862   0.681   0.907   0.912   0.671   0.844   0.845   0.525
191   K        -0.620  -0.635  -0.716  -0.379  -0.401  -0.539  -0.555  -0.572  -0.652
192   A        -0.188  -0.182  -0.200  -0.220  -0.207  -0.235  -0.355  -0.346  -0.362
193   P        -0.783  -0.773  -0.777  -0.653  -0.634  -0.652  -0.755  -0.744  -0.721
194   K         0.861   0.883   0.643   0.714   0.743   0.432   0.844   0.878   0.457
195   I        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
196   Q        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
197   I         0.407   0.411   0.471   0.338   0.345   0.437   0.045   0.050   0.136
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/Q08422
Output conservation file - output/Q08422
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
