1     M         0.921   0.946   1.007   1.180   1.205   1.274   0.953   0.995   1.081
2     G        -0.251  -0.156  -0.309  -0.235  -0.105  -0.332  -0.349  -0.227  -0.489
3     K        -0.509  -0.532  -0.389  -0.842  -0.873  -0.597  -0.756  -0.831  -0.623
4     S         0.160  -0.129  -0.318   0.402   0.039  -0.312   0.221  -0.188  -0.505
5     H        -2.075  -2.030  -1.776  -1.997  -1.938  -1.617  -1.895  -1.804  -1.471
6     G         0.921   0.946   1.007   0.847   0.902   1.000   0.953   0.995   1.081
7     Y        -0.956  -1.126  -1.166  -0.730  -0.968  -1.063  -0.919  -1.120  -1.147
8     R         0.921   0.946   1.007   0.762   0.808   0.930   0.953   0.995   1.081
9     S        -0.509  -0.532  -0.389  -0.545  -0.643  -0.457  -0.756  -0.831  -0.623
10    R        -0.655  -0.564  -0.392  -1.054  -0.929  -0.636  -1.000  -0.886  -0.628
11    T         0.921   0.946   1.007   0.967   1.003   1.098   0.953   0.995   1.081
12    R         0.921   0.946   1.007   0.762   0.808   0.930   0.953   0.995   1.081
13    Y         0.921   0.946   1.007   0.959   1.005   1.092   0.953   0.995   1.081
14    M        -2.197  -2.003  -1.768  -1.911  -1.741  -1.452  -1.977  -1.784  -1.464
15    F         0.921   0.946   1.007   1.138   1.171   1.240   0.953   0.995   1.081
16    Q        -0.251  -0.469  -0.390  -0.066  -0.434  -0.367  -0.349  -0.726  -0.625
17    R         0.921   0.946   1.007   0.762   0.808   0.930   0.953   0.995   1.081
18    D        -0.956  -1.126  -1.166  -0.569  -0.828  -0.980  -0.919  -1.120  -1.147
19    F         0.921   0.946   1.007   1.138   1.171   1.240   0.953   0.995   1.081
20    R        -0.655  -0.529  -0.387  -1.197  -0.998  -0.769  -1.000  -0.826  -0.620
21    K        -0.509  -0.532  -0.389  -0.866  -0.907  -0.630  -0.756  -0.831  -0.623
22    H        -0.575  -0.982  -1.162  -0.151  -0.737  -1.113  -0.430  -0.942  -1.142
23    G         0.921   0.946   1.007   0.847   0.902   1.000   0.953   0.995   1.081
24    A        -1.628  -1.876  -1.773  -1.312  -1.636  -1.475  -1.407  -1.649  -1.468
25    V         0.160   0.358  -0.288   0.300   0.507  -0.400   0.221   0.464  -0.456
26    H        -2.075  -1.743  -1.769  -1.990  -1.645  -1.574  -1.895  -1.591  -1.465
27    L         0.160   0.018  -0.310   0.452   0.290  -0.162   0.221   0.024  -0.492
28    S         0.921   0.946   1.007   1.010   1.060   1.134   0.953   0.995   1.081
29    T         0.160   0.018  -0.310   0.336   0.152  -0.417   0.221   0.024  -0.492
30    Y         0.921   0.946   1.007   0.959   1.005   1.092   0.953   0.995   1.081
31    L         0.160   0.315  -0.290   0.452   0.611  -0.131   0.221   0.412  -0.458
32    K         0.921   0.946   1.007   0.605   0.665   0.800   0.953   0.995   1.081
33    V        -1.287  -1.318  -1.177  -1.467  -1.402  -1.206  -1.407  -1.375  -1.161
34    Y         0.921   0.946   1.007   0.959   1.005   1.092   0.953   0.995   1.081
35    K        -0.251  -0.016  -0.297  -0.556  -0.220  -0.627  -0.349  -0.023  -0.470
36    V         0.921   0.946   1.007   0.659   0.729   0.845   0.953   0.995   1.081
37    G         0.921   0.946   1.007   0.847   0.902   1.000   0.953   0.995   1.081
38    D         0.921   0.946   1.007   1.149   1.172   1.249   0.953   0.995   1.081
39    I         0.921   0.946   1.007   0.931   0.955   1.069   0.953   0.995   1.081
40    V         0.921   0.946   1.007   0.659   0.729   0.845   0.953   0.995   1.081
41    D         0.921   0.946   1.007   1.149   1.172   1.249   0.953   0.995   1.081
42    I         0.921   0.946   1.007   0.931   0.955   1.069   0.953   0.995   1.081
43    K         0.160   0.018  -0.310  -0.018  -0.175  -0.650   0.221   0.024  -0.492
44    A        -0.509  -0.532  -0.389  -0.759  -0.872  -0.666  -0.756  -0.831  -0.623
45    N         0.921   0.946   1.007   1.018   1.059   1.141   0.953   0.995   1.081
46    G         0.921   0.946   1.007   0.847   0.902   1.000   0.953   0.995   1.081
47    S        -0.509  -0.532  -0.389  -0.545  -0.643  -0.457  -0.756  -0.831  -0.623
48    I        -0.509  -0.532  -0.389  -0.705  -0.836  -0.629  -0.756  -0.831  -0.623
49    Q         0.160   0.315  -0.290   0.452   0.622  -0.162   0.221   0.412  -0.458
50    K         0.921   0.946   1.007   0.605   0.665   0.800   0.953   0.995   1.081
51    G         0.921   0.946   1.007   0.847   0.902   1.000   0.953   0.995   1.081
52    M         0.921   0.946   1.007   1.180   1.205   1.274   0.953   0.995   1.081
53    P         0.160  -0.129  -0.318   0.469   0.094  -0.264   0.221  -0.188  -0.505
54    H         0.921   0.946   1.007   1.076   1.114   1.189   0.953   0.995   1.081
55    K         0.921   0.946   1.007   0.605   0.665   0.800   0.953   0.995   1.081
56    F        -1.287  -1.367  -1.187  -1.227  -1.352  -1.134  -1.407  -1.443  -1.173
57    Y         0.921   0.946   1.007   0.959   1.005   1.092   0.953   0.995   1.081
58    Q        -0.702  -0.528  -0.368  -0.770  -0.499  -0.286  -1.081  -0.824  -0.587
59    G         0.921   0.946   1.007   0.847   0.902   1.000   0.953   0.995   1.081
60    K         0.160   0.018  -0.310  -0.018  -0.175  -0.650   0.221   0.024  -0.492
61    T         0.921   0.946   1.007   0.967   1.003   1.098   0.953   0.995   1.081
62    G         0.921   0.946   1.007   0.847   0.902   1.000   0.953   0.995   1.081
63    V        -0.655  -0.529  -0.387  -1.094  -0.905  -0.648  -1.000  -0.826  -0.620
64    V         0.160  -0.129  -0.318   0.057  -0.279  -0.548   0.221  -0.188  -0.505
65    Y         0.921   0.946   1.007   0.959   1.005   1.092   0.953   0.995   1.081
66    N         0.921   0.946   1.007   1.018   1.059   1.141   0.953   0.995   1.081
67    V         0.921   0.946   1.007   0.659   0.729   0.845   0.953   0.995   1.081
68    T         0.921   0.946   1.007   0.967   1.003   1.098   0.953   0.995   1.081
69    K        -0.251  -0.156  -0.309  -0.484  -0.347  -0.527  -0.349  -0.227  -0.489
70    S         0.921   0.946   1.007   1.010   1.060   1.134   0.953   0.995   1.081
71    S        -0.655  -0.587  -0.390  -0.824  -0.771  -0.468  -1.000  -0.927  -0.625
72    V         0.921   0.946   1.007   0.659   0.729   0.845   0.953   0.995   1.081
73    G         0.921   0.946   1.007   0.847   0.902   1.000   0.953   0.995   1.081
74    V        -0.251  -0.350  -0.382  -0.458  -0.609  -0.643  -0.349  -0.529  -0.612
75    I        -0.251  -0.156  -0.309  -0.166  -0.067  -0.292  -0.349  -0.227  -0.489
76    I         0.160   0.386  -0.287   0.300   0.536  -0.398   0.221   0.496  -0.455
77    N        -0.702  -0.579  -0.389  -0.868  -0.695  -0.411  -1.081  -0.912  -0.624
78    K         0.921   0.946   1.007   0.605   0.665   0.800   0.953   0.995   1.081
79    M        -1.181  -1.192  -1.161  -1.321  -1.356  -1.306  -1.244  -1.206  -1.141
80    V         0.921   0.946   1.007   0.659   0.729   0.845   0.953   0.995   1.081
81    G         0.160  -0.129  -0.318   0.210  -0.174  -0.548   0.221  -0.188  -0.505
82    N         0.160  -0.129  -0.318   0.432   0.083  -0.223   0.221  -0.188  -0.505
83    R         0.921   0.946   1.007   0.762   0.808   0.930   0.953   0.995   1.081
84    Y         0.160   0.018  -0.310   0.381   0.229  -0.227   0.221   0.024  -0.492
85    L        -1.489  -1.462  -1.210  -1.345  -1.369  -1.020  -1.570  -1.542  -1.198
86    E         0.921   0.946   1.007   1.022   1.056   1.144   0.953   0.995   1.081
87    K         0.921   0.946   1.007   0.605   0.665   0.800   0.953   0.995   1.081
88    R         0.921   0.946   1.007   0.762   0.808   0.930   0.953   0.995   1.081
89    L        -1.628  -1.356  -1.184  -1.868  -1.484  -1.243  -1.733  -1.413  -1.169
90    N         0.921   0.946   1.007   1.018   1.059   1.141   0.953   0.995   1.081
91    L        -0.509  -0.532  -0.389  -0.614  -0.741  -0.566  -0.756  -0.831  -0.623
92    R         0.921   0.946   1.007   0.762   0.808   0.930   0.953   0.995   1.081
93    V        -0.655  -0.582  -0.382  -1.094  -0.962  -0.610  -1.000  -0.918  -0.612
94    E         0.921   0.946   1.007   1.022   1.056   1.144   0.953   0.995   1.081
95    H         0.921   0.946   1.007   1.076   1.114   1.189   0.953   0.995   1.081
96    I        -0.655  -0.483  -0.380  -1.014  -0.731  -0.604  -1.000  -0.749  -0.608
97    K         0.160  -0.129  -0.318   0.015  -0.312  -0.540   0.221  -0.188  -0.505
98    H         0.160  -0.129  -0.318   0.490   0.138  -0.181   0.221  -0.188  -0.505
99    S         0.921   0.946   1.007   1.010   1.060   1.134   0.953   0.995   1.081
100   K        -1.181  -1.212  -1.173  -1.499  -1.522  -1.441  -1.244  -1.232  -1.156
101   C         0.160  -0.129  -0.318   0.677   0.303  -0.068   0.221  -0.188  -0.505
102   R         0.921   0.946   1.007   0.762   0.808   0.930   0.953   0.995   1.081
103   Q         0.160  -0.129  -0.318   0.460   0.119  -0.179   0.221  -0.188  -0.505
104   E        -0.509  -0.532  -0.389  -0.425  -0.507  -0.297  -0.756  -0.831  -0.623
105   F         0.921   0.946   1.007   1.138   1.171   1.240   0.953   0.995   1.081
106   L        -0.251  -0.016  -0.297  -0.096   0.203  -0.239  -0.349  -0.023  -0.470
107   E        -2.075  -1.958  -1.766  -1.793  -1.682  -1.459  -1.895  -1.735  -1.462
108   R         0.921   0.946   1.007   0.762   0.808   0.930   0.953   0.995   1.081
109   V         0.921   0.946   1.007   0.659   0.729   0.845   0.953   0.995   1.081
110   K         0.921   0.946   1.007   0.605   0.665   0.800   0.953   0.995   1.081
111   A        -1.287  -1.370  -1.182  -1.295  -1.382  -1.100  -1.407  -1.448  -1.168
112   N         0.921   0.946   1.007   1.018   1.059   1.141   0.953   0.995   1.081
113   A        -0.956  -1.005  -1.151  -0.768  -0.831  -1.007  -0.919  -0.972  -1.129
114   A        -0.575  -0.982  -1.162  -0.300  -0.824  -1.072  -0.430  -0.942  -1.142
115   K        -0.509  -0.482  -0.384  -0.864  -0.831  -0.626  -0.756  -0.747  -0.616
116   R        -0.702  -0.587  -0.392  -1.321  -1.148  -0.788  -1.081  -0.927  -0.628
117   A        -1.628  -1.293  -1.176  -2.148  -1.655  -1.451  -1.733  -1.331  -1.159
118   E         0.160  -0.129  -0.318   0.432   0.073  -0.236   0.221  -0.188  -0.505
119   A         0.921   0.946   1.007   0.875   0.915   1.023   0.953   0.995   1.081
120   K         0.921   0.946   1.007   0.605   0.665   0.800   0.953   0.995   1.081
121   A         0.921   0.946   1.007   0.875   0.915   1.023   0.953   0.995   1.081
122   Q        -1.814  -1.937  -1.774  -1.561  -1.747  -1.598  -1.651  -1.716  -1.469
123   G         0.160   0.018  -0.310   0.255   0.107  -0.358   0.221   0.024  -0.492
124   V        -1.548  -1.302  -1.184  -1.923  -1.596  -1.405  -1.651  -1.380  -1.170
125   A        -2.601  -2.553  -2.229  -2.330  -2.267  -1.818  -2.140  -2.037  -1.646
126   V         0.921   0.946   1.007   0.659   0.729   0.845   0.953   0.995   1.081
127   Q        -0.956  -0.600  -1.123  -0.623  -0.191  -0.913  -0.919  -0.491  -1.094
128   L         0.921   0.946   1.007   1.026   1.061   1.147   0.953   0.995   1.081
129   K        -0.509  -0.481  -0.383  -0.978  -0.960  -0.778  -0.756  -0.746  -0.614
130   R         0.921   0.946   1.007   0.762   0.808   0.930   0.953   0.995   1.081
131   Q         0.921   0.946   1.007   1.037   1.081   1.157   0.953   0.995   1.081
132   P        -0.251  -0.344  -0.382  -0.060  -0.242  -0.396  -0.349  -0.520  -0.611
133   A        -1.628  -1.784  -1.759  -1.480  -1.691  -1.719  -1.407  -1.552  -1.455
134   Q        -2.601  -2.587  -2.238  -2.483  -2.441  -1.960  -2.140  -2.062  -1.653
135   P         0.160   0.018  -0.310   0.478   0.309  -0.212   0.221   0.024  -0.492
136   R        -0.956  -0.744  -1.128  -0.999  -0.742  -1.326  -0.919  -0.684  -1.101
137   E        -2.261  -1.871  -2.191  -1.792  -1.328  -1.865  -1.814  -1.428  -1.617
138   S        -0.509  -0.266  -0.348  -0.617  -0.264  -0.403  -0.756  -0.396  -0.554
139   R        -0.956  -1.005  -1.151  -0.940  -1.029  -1.279  -0.919  -0.972  -1.129
140   I        -2.197  -2.016  -1.776  -2.261  -2.010  -1.635  -1.977  -1.800  -1.471
141   V        -0.956  -1.159  -1.181  -1.024  -1.307  -1.269  -0.919  -1.183  -1.167
142   S        -0.956  -0.883  -1.138  -0.691  -0.589  -1.028  -0.919  -0.815  -1.113
143   T        -0.575  -0.982  -1.162  -0.265  -0.851  -1.201  -0.430  -0.942  -1.142
144   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
145   E        -0.956  -1.011  -1.147  -0.807  -0.906  -1.267  -0.919  -0.976  -1.124
146   G        -2.522  -2.533  -2.233  -2.215  -2.213  -1.800  -2.058  -2.020  -1.649
147   N         0.921   0.946   1.007   1.018   1.059   1.141   0.953   0.995   1.081
148   V        -2.261  -2.347  -2.231  -2.176  -2.395  -2.152  -1.814  -1.885  -1.648
149   P         0.921   0.946   1.007   1.076   1.115   1.189   0.953   0.995   1.081
150   Q        -1.628  -1.876  -1.773  -1.292  -1.659  -1.578  -1.407  -1.649  -1.468
151   T         0.921   0.946   1.007   0.967   1.003   1.098   0.953   0.995   1.081
152   L        -0.251  -0.469  -0.390  -0.161  -0.603  -0.571  -0.349  -0.726  -0.625
153   A        -0.251  -0.156  -0.309  -0.208  -0.093  -0.311  -0.349  -0.227  -0.489
154   P         0.921   0.946   1.007   1.076   1.115   1.189   0.953   0.995   1.081
155   V         0.160   0.018  -0.310   0.068  -0.065  -0.493   0.221   0.024  -0.492
156   P        -0.655  -0.431  -0.356  -0.824  -0.497  -0.384  -1.000  -0.662  -0.568
157   Y         0.921   0.946   1.007   0.959   1.005   1.092   0.953   0.995   1.081
158   E        -0.251  -0.156  -0.309  -0.047   0.059  -0.175  -0.349  -0.227  -0.489
159   T         0.921   0.946   1.007   0.967   1.003   1.098   0.953   0.995   1.081
160   F        -0.509  -0.532  -0.389  -0.389  -0.500  -0.325  -0.756  -0.831  -0.623
161   I         0.921   0.946   1.007   0.931   0.955   1.069   0.953   0.995   1.081
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/Q02753
Output conservation file - output/Q02753
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
