1     M         1.047   1.089   1.165   1.366   1.397   1.480   1.094   1.151   1.261
2     S        -0.550  -0.254  -0.801  -0.324   0.044  -0.729  -0.524  -0.145  -0.831
3     A        -2.318  -2.268  -1.897  -2.292  -2.218  -1.751  -1.995  -1.876  -1.439
4     Q        -1.846  -1.728  -1.462  -1.903  -1.768  -1.458  -1.774  -1.615  -1.259
5     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
6     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
7     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
8     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
9     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
10    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
11    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
12    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
13    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
14    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
15    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
16    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
17    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
18    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
19    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
20    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
21    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
22    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
23    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
24    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
25    K        -1.963  -1.650  -1.844  -2.032  -1.665  -1.809  -1.645  -1.328  -1.397
26    A         0.229   0.408  -0.027   0.152   0.341  -0.167   0.223   0.467  -0.205
27    P        -1.069  -1.106  -0.895  -1.036  -1.091  -0.821  -1.271  -1.263  -0.954
28    K         1.047   1.089   1.165   0.648   0.727   0.881   1.094   1.151   1.261
29    W         0.229   0.070  -0.057   0.723   0.510   0.263   0.223  -0.018  -0.256
30    Y         1.047   1.089   1.165   1.262   1.300   1.392   1.094   1.151   1.261
31    P        -0.908  -0.684  -0.843  -0.769  -0.447  -0.743  -1.022  -0.691  -0.886
32    S        -0.389  -0.130  -0.104  -0.758  -0.364  -0.305  -0.773  -0.341  -0.338
33    E         0.229   0.070  -0.057   0.437   0.257   0.049   0.223  -0.018  -0.256
34    D        -1.284  -1.320  -1.419  -0.804  -0.894  -1.109  -1.147  -1.182  -1.218
35    V        -1.587  -1.505  -1.442  -1.613  -1.470  -1.376  -1.520  -1.433  -1.243
36    A        -1.679  -1.672  -1.445  -1.629  -1.584  -1.206  -1.645  -1.564  -1.244
37    A        -1.069  -1.029  -0.872  -1.383  -1.217  -0.987  -1.271  -1.149  -0.925
38    L        -0.682  -0.349  -0.829  -0.528  -0.085  -0.878  -0.694  -0.262  -0.867
39    K        -0.252  -0.254  -0.149  -0.715  -0.701  -0.511  -0.532  -0.554  -0.416
40    K        -0.389  -0.145  -0.106  -0.834  -0.461  -0.366  -0.773  -0.365  -0.341
41    T        -1.284  -1.570  -1.448  -0.892  -1.387  -1.286  -1.147  -1.446  -1.246
42    R         0.229   0.223  -0.043   0.350   0.350  -0.039   0.223   0.211  -0.232
43    K         0.229   0.223  -0.043  -0.023   0.006  -0.300   0.223   0.211  -0.232
44    A        -1.069  -1.010  -0.890  -1.189  -1.122  -0.964  -1.271  -1.164  -0.948
45    A        -1.963  -2.064  -1.883  -1.785  -1.948  -1.635  -1.645  -1.715  -1.428
46    R         0.229   0.223  -0.043   0.365   0.369  -0.002   0.223   0.211  -0.232
47    P         0.229   0.223  -0.043   0.352   0.338  -0.156   0.223   0.211  -0.232
48    Q        -1.284  -1.570  -1.448  -0.732  -1.100  -1.008  -1.147  -1.446  -1.246
49    K         0.229   0.054  -0.056  -0.042  -0.233  -0.372   0.223  -0.043  -0.254
50    L         0.229   0.054  -0.056   0.148  -0.038  -0.215   0.223  -0.043  -0.254
51    R         1.047   1.089   1.165   1.034   1.087   1.202   1.094   1.151   1.261
52    A        -0.550  -0.740  -0.852  -0.525  -0.794  -0.919  -0.524  -0.768  -0.897
53    S         0.229   0.054  -0.056   0.388   0.181  -0.008   0.223  -0.043  -0.254
54    L         1.047   1.089   1.165   0.831   0.917   1.033   1.094   1.151   1.261
55    V         0.229   0.070  -0.057   0.149  -0.062  -0.293   0.223  -0.018  -0.256
56    P         1.047   1.089   1.165   1.097   1.143   1.255   1.094   1.151   1.261
57    G         1.047   1.089   1.165   1.171   1.207   1.317   1.094   1.151   1.261
58    T         0.229   0.223  -0.043   0.464   0.447  -0.020   0.223   0.211  -0.232
59    V         1.047   1.089   1.165   0.870   0.937   1.066   1.094   1.151   1.261
60    L         0.229   0.070  -0.057   0.197   0.059  -0.083   0.223  -0.018  -0.256
61    I         1.047   1.089   1.165   1.241   1.269   1.375   1.094   1.151   1.261
62    L         1.047   1.089   1.165   0.831   0.917   1.033   1.094   1.151   1.261
63    L         1.047   1.089   1.165   0.831   0.917   1.033   1.094   1.151   1.261
64    A         1.047   1.089   1.165   0.835   0.866   1.037   1.094   1.151   1.261
65    G         1.047   1.089   1.165   1.171   1.207   1.317   1.094   1.151   1.261
66    R         1.047   1.089   1.165   1.034   1.087   1.202   1.094   1.151   1.261
67    F         1.047   1.089   1.165   1.180   1.227   1.324   1.094   1.151   1.261
68    R         1.047   1.089   1.165   1.034   1.087   1.202   1.094   1.151   1.261
69    G         1.047   1.089   1.165   1.171   1.207   1.317   1.094   1.151   1.261
70    K         1.047   1.089   1.165   0.648   0.727   0.881   1.094   1.151   1.261
71    R         1.047   1.089   1.165   1.034   1.087   1.202   1.094   1.151   1.261
72    V         1.047   1.089   1.165   0.870   0.937   1.066   1.094   1.151   1.261
73    V         1.047   1.089   1.165   0.870   0.937   1.066   1.094   1.151   1.261
74    Y        -0.908  -1.084  -0.902  -0.755  -1.115  -0.944  -1.022  -1.231  -0.962
75    L         1.047   1.089   1.165   0.831   0.917   1.033   1.094   1.151   1.261
76    K         0.229   0.070  -0.057  -0.042  -0.212  -0.373   0.223  -0.018  -0.256
77    H        -2.198  -2.220  -1.891  -1.681  -1.773  -1.401  -1.894  -1.837  -1.434
78    L         0.229   0.408  -0.027   0.189   0.421  -0.066   0.223   0.467  -0.205
79    E         0.229   0.054  -0.056   0.431   0.226   0.035   0.223  -0.043  -0.254
80    D         0.229   0.054  -0.056   0.553   0.328   0.125   0.223  -0.043  -0.254
81    N        -0.252  -0.341  -0.156  -0.144  -0.320  -0.085  -0.532  -0.707  -0.428
82    T         0.229   0.054  -0.056   0.469   0.222  -0.033   0.223  -0.043  -0.254
83    L         1.047   1.089   1.165   0.831   0.917   1.033   1.094   1.151   1.261
84    L         0.229   0.070  -0.057   0.114  -0.066  -0.309   0.223  -0.018  -0.256
85    I         0.229   0.436  -0.025   0.209   0.455  -0.082   0.223   0.504  -0.201
86    S        -0.389  -0.359  -0.147  -0.494  -0.439  -0.137  -0.773  -0.740  -0.412
87    G         1.047   1.089   1.165   1.171   1.207   1.317   1.094   1.151   1.261
88    P         1.047   1.089   1.165   1.097   1.143   1.255   1.094   1.151   1.261
89    F         0.229   0.070  -0.057   0.519   0.325   0.111   0.223  -0.018  -0.256
90    K         1.047   1.089   1.165   0.648   0.727   0.881   1.094   1.151   1.261
91    V         0.229   0.054  -0.056   0.209   0.014  -0.126   0.223  -0.043  -0.254
92    N         1.047   1.089   1.165   1.196   1.255   1.338   1.094   1.151   1.261
93    G         1.047   1.089   1.165   1.171   1.207   1.317   1.094   1.151   1.261
94    V         1.047   1.089   1.165   0.870   0.937   1.066   1.094   1.151   1.261
95    P         1.047   1.089   1.165   1.097   1.143   1.255   1.094   1.151   1.261
96    L         0.229   0.070  -0.057   0.170   0.014  -0.157   0.223  -0.018  -0.256
97    R         1.047   1.089   1.165   1.034   1.087   1.202   1.094   1.151   1.261
98    R         1.047   1.089   1.165   1.034   1.087   1.202   1.094   1.151   1.261
99    V         1.047   1.089   1.165   0.870   0.937   1.066   1.094   1.151   1.261
100   N         1.047   1.089   1.165   1.196   1.255   1.338   1.094   1.151   1.261
101   A        -0.550  -0.541  -0.833  -0.476  -0.477  -0.784  -0.524  -0.532  -0.873
102   R         1.047   1.089   1.165   1.034   1.087   1.202   1.094   1.151   1.261
103   Y         1.047   1.089   1.165   1.262   1.300   1.392   1.094   1.151   1.261
104   V         1.047   1.089   1.165   0.870   0.937   1.066   1.094   1.151   1.261
105   I         1.047   1.089   1.165   1.241   1.269   1.375   1.094   1.151   1.261
106   A         1.047   1.089   1.165   0.835   0.866   1.037   1.094   1.151   1.261
107   T         1.047   1.089   1.165   1.184   1.229   1.328   1.094   1.151   1.261
108   S         1.047   1.089   1.165   1.059   1.118   1.223   1.094   1.151   1.261
109   T        -0.550  -0.868  -0.870  -0.194  -0.653  -0.748  -0.524  -0.930  -0.919
110   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
111   K         0.229   0.403  -0.025  -0.046   0.189  -0.335   0.223   0.461  -0.202
112   V         0.229   0.070  -0.057   0.209   0.034  -0.127   0.223  -0.018  -0.256
113   S        -1.211  -0.827  -0.865  -1.080  -0.504  -0.642  -1.396  -0.877  -0.913
114   V        -0.389  -0.318  -0.151  -0.852  -0.720  -0.483  -0.773  -0.666  -0.420
115   E        -1.069  -1.047  -0.879  -1.141  -1.084  -0.888  -1.271  -1.175  -0.933
116   G         1.047   1.089   1.165   1.171   1.207   1.317   1.094   1.151   1.261
117   V         0.229   0.054  -0.056   0.209   0.014  -0.126   0.223  -0.043  -0.254
118   N        -1.587  -1.570  -1.438  -1.318  -1.300  -1.245  -1.520  -1.445  -1.237
119   V        -1.284  -1.332  -1.417  -1.170  -1.250  -1.368  -1.147  -1.196  -1.216
120   E        -1.821  -1.660  -1.448  -1.832  -1.606  -1.304  -1.769  -1.555  -1.248
121   K         1.047   1.089   1.165   0.648   0.727   0.881   1.094   1.151   1.261
122   F         0.229   0.054  -0.056   0.516   0.297   0.104   0.223  -0.043  -0.254
123   N        -0.174  -0.247  -0.144  -0.017  -0.143  -0.058  -0.400  -0.540  -0.408
124   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
125   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
126   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
127   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
128   V        -0.908  -1.084  -0.902  -1.073  -1.354  -1.114  -1.022  -1.231  -0.962
129   E        -1.069  -1.108  -0.890  -1.310  -1.436  -1.122  -1.271  -1.263  -0.947
130   Y         1.047   1.089   1.165   1.262   1.300   1.392   1.094   1.151   1.261
131   F         1.047   1.089   1.165   1.180   1.227   1.324   1.094   1.151   1.261
132   A        -0.550  -0.583  -0.834  -0.510  -0.576  -0.859  -0.524  -0.582  -0.874
133   K        -0.908  -1.041  -0.893  -1.051  -1.310  -1.177  -1.022  -1.166  -0.951
134   E        -0.550  -0.727  -0.854  -0.245  -0.456  -0.696  -0.524  -0.749  -0.899
135   K         1.047   1.089   1.165   0.660   0.741   0.891   1.094   1.151   1.261
136   L        -1.651  -1.526  -1.463  -1.790  -1.611  -1.531  -1.562  -1.431  -1.261
137   T        -1.284  -0.834  -1.368  -0.913  -0.360  -1.049  -1.147  -0.619  -1.166
138   K        -1.587  -1.571  -1.436  -1.858  -1.786  -1.562  -1.520  -1.446  -1.235
139   K        -2.198  -2.220  -1.891  -2.261  -2.183  -1.700  -1.894  -1.837  -1.434
140   E        -1.587  -1.440  -1.428  -1.805  -1.597  -1.493  -1.520  -1.320  -1.228
141   K        -0.550  -0.583  -0.834  -0.767  -0.827  -1.149  -0.524  -0.582  -0.874
142   K        -1.821  -1.710  -1.449  -2.208  -2.008  -1.583  -1.769  -1.595  -1.249
143   E         0.229   0.070  -0.057   0.431   0.247   0.033   0.223  -0.018  -0.256
144   A        -0.908  -1.010  -0.898  -1.107  -1.278  -1.120  -1.022  -1.164  -0.957
145   N        -1.963  -1.709  -1.462  -1.922  -1.637  -1.263  -1.894  -1.593  -1.260
146   L        -0.550  -0.224  -0.802  -0.174   0.216  -0.580  -0.524  -0.108  -0.832
147   F         1.047   1.089   1.165   1.180   1.227   1.324   1.094   1.151   1.261
148   P        -1.821  -1.758  -1.462  -1.879  -1.914  -1.483  -1.769  -1.643  -1.260
149   E         0.229   0.054  -0.056   0.430   0.228   0.033   0.223  -0.043  -0.254
150   Q        -2.716  -2.273  -2.221  -2.236  -1.678  -1.639  -2.143  -1.756  -1.538
151   Q        -1.963  -2.080  -1.885  -1.593  -1.883  -1.726  -1.645  -1.727  -1.429
152   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
153   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
154   -        -0.039  -0.143  -0.103   0.080  -0.075  -0.052  -0.180  -0.362  -0.336
155   N        -1.284  -1.018  -1.387  -1.318  -0.961  -1.346  -1.147  -0.835  -1.186
156   K         0.229   0.070  -0.057  -0.021  -0.176  -0.314   0.223  -0.018  -0.256
157   E        -0.550  -0.565  -0.836  -0.281  -0.341  -0.749  -0.524  -0.557  -0.876
158   I        -1.211  -1.204  -0.928  -1.291  -1.386  -1.024  -1.396  -1.369  -0.991
159   K        -0.550  -0.868  -0.870  -0.715  -1.100  -1.071  -0.524  -0.930  -0.919
160   A         0.229   0.054  -0.056   0.147  -0.094  -0.227   0.223  -0.043  -0.254
161   E         0.229   0.054  -0.056   0.408   0.192  -0.028   0.223  -0.043  -0.254
162   R         1.047   1.089   1.165   1.034   1.087   1.202   1.094   1.151   1.261
163   V        -0.174  -0.175  -0.136  -0.309  -0.304  -0.231  -0.400  -0.416  -0.393
164   E        -0.389  -0.182  -0.114  -0.737  -0.429  -0.301  -0.773  -0.428  -0.355
165   D         1.047   1.089   1.165   1.221   1.259   1.358   1.094   1.151   1.261
166   Q         1.047   1.089   1.165   1.217   1.266   1.355   1.094   1.151   1.261
167   K         1.047   1.089   1.165   0.648   0.727   0.881   1.094   1.151   1.261
168   V        -2.340  -2.047  -1.876  -2.252  -1.893  -1.637  -2.018  -1.699  -1.423
169   V        -0.174  -0.184  -0.138  -0.309  -0.318  -0.233  -0.400  -0.432  -0.396
170   D         1.047   1.089   1.165   1.221   1.259   1.358   1.094   1.151   1.261
171   K        -0.458  -0.363  -0.156  -1.170  -1.013  -0.632  -0.898  -0.746  -0.428
172   A        -0.550  -0.541  -0.833  -0.602  -0.664  -1.069  -0.524  -0.532  -0.873
173   L         1.047   1.089   1.165   0.831   0.917   1.033   1.094   1.151   1.261
174   I        -0.389  -0.345  -0.156  -0.660  -0.563  -0.263  -0.773  -0.714  -0.428
175   A        -0.908  -0.815  -0.856  -1.035  -0.890  -0.925  -1.022  -0.860  -0.902
176   E        -0.908  -1.043  -0.896  -0.783  -0.994  -0.841  -1.022  -1.172  -0.954
177   I         1.047   1.089   1.165   1.241   1.269   1.375   1.094   1.151   1.261
178   K         1.047   1.089   1.165   0.648   0.727   0.881   1.094   1.151   1.261
179   K         0.229   0.070  -0.057  -0.076  -0.274  -0.469   0.223  -0.018  -0.256
180   T        -0.550  -0.868  -0.870  -0.185  -0.636  -0.725  -0.524  -0.930  -0.919
181   P         0.229   0.054  -0.056   0.413   0.189  -0.014   0.223  -0.043  -0.254
182   L        -0.550  -0.868  -0.870  -0.491  -0.847  -0.852  -0.524  -0.930  -0.919
183   L         0.229   0.070  -0.057   0.189   0.046  -0.104   0.223  -0.018  -0.256
184   K         0.229   0.054  -0.056  -0.076  -0.298  -0.468   0.223  -0.043  -0.254
185   Q        -0.550  -0.868  -0.870  -0.147  -0.582  -0.688  -0.524  -0.930  -0.919
186   Y         1.047   1.089   1.165   1.262   1.300   1.392   1.094   1.151   1.261
187   L         1.047   1.089   1.165   0.831   0.917   1.033   1.094   1.151   1.261
188   S        -0.174   0.075  -0.087  -0.405  -0.066  -0.277  -0.400  -0.011  -0.308
189   A        -0.174  -0.254  -0.145  -0.405  -0.561  -0.365  -0.400  -0.553  -0.408
190   S        -0.550  -0.428  -0.820  -0.261  -0.104  -0.626  -0.524  -0.367  -0.855
191   F         1.047   1.089   1.165   1.180   1.227   1.324   1.094   1.151   1.261
192   S        -0.550  -0.868  -0.870  -0.324  -0.771  -0.846  -0.524  -0.930  -0.919
193   L         1.047   1.089   1.165   0.831   0.917   1.033   1.094   1.151   1.261
194   K        -0.550  -0.868  -0.870  -0.726  -1.119  -1.099  -0.524  -0.930  -0.919
195   N        -0.908  -0.972  -0.884  -0.774  -0.886  -0.893  -1.022  -1.070  -0.939
196   G         1.047   1.089   1.165   1.171   1.207   1.317   1.094   1.151   1.261
197   D         1.047   1.089   1.165   1.221   1.259   1.358   1.094   1.151   1.261
198   K        -0.389  -0.293  -0.147  -0.940  -0.766  -0.508  -0.773  -0.621  -0.412
199   P         1.047   1.089   1.165   1.097   1.143   1.255   1.094   1.151   1.261
200   H         1.047   1.089   1.165   1.370   1.404   1.483   1.094   1.151   1.261
201   M        -0.550  -0.554  -0.832  -0.475  -0.476  -0.774  -0.524  -0.550  -0.871
202   L        -0.174  -0.308  -0.154  -0.303  -0.443  -0.191  -0.400  -0.648  -0.425
203   K         0.229   0.408  -0.027  -0.021   0.215  -0.273   0.223   0.467  -0.205
204   F         0.229   0.054  -0.056   0.537   0.333   0.160   0.223  -0.043  -0.254
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/Q02326
Output conservation file - output/Q02326
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
