1     M         1.191   1.201   1.251   1.301   1.317   1.387   1.268   1.281   1.353
2     S        -0.197  -0.291  -0.365  -0.235  -0.367  -0.496  -0.321  -0.441  -0.553
3     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
4     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
5     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
6     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
7     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
8     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
9     Q         0.373   0.314   0.256   0.464   0.373   0.258   0.238   0.132  -0.012
10    P        -0.363  -0.483  -0.419  -0.381  -0.605  -0.538  -0.534  -0.705  -0.627
11    V        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
12    Q        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
13    R        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
14    A         0.213   0.143   0.197  -0.074  -0.197  -0.102  -0.045  -0.181  -0.126
15    A        -1.551  -1.534  -1.493  -1.541  -1.517  -1.435  -1.274  -1.260  -1.153
16    A        -1.646  -1.616  -1.502  -1.619  -1.590  -1.422  -1.354  -1.324  -1.159
17    R        -0.796  -0.787  -0.831  -0.780  -0.753  -0.784  -0.877  -0.867  -0.859
18    S        -1.048  -1.044  -0.888  -1.218  -1.189  -0.966  -1.116  -1.108  -0.914
19    F        -0.891  -0.887  -0.854  -0.977  -0.989  -0.931  -0.957  -0.941  -0.881
20    L        -0.953  -0.928  -0.862  -1.008  -0.989  -0.876  -1.036  -1.009  -0.889
21    Q        -1.646  -1.659  -1.514  -1.498  -1.508  -1.318  -1.354  -1.352  -1.168
22    K        -0.688  -0.752  -0.819  -0.665  -0.703  -0.810  -0.718  -0.776  -0.840
23    Y        -0.606  -0.591  -0.448  -0.855  -0.860  -0.611  -0.877  -0.855  -0.666
24    I        -0.953  -0.997  -0.874  -0.976  -1.048  -0.858  -1.036  -1.066  -0.901
25    N         0.150   0.199   0.219  -0.005   0.061   0.094  -0.162  -0.075  -0.084
26    K        -0.449  -0.551  -0.790  -0.288  -0.387  -0.718  -0.400  -0.525  -0.806
27    E        -1.048  -1.044  -0.888  -0.919  -0.909  -0.705  -1.116  -1.108  -0.914
28    T        -1.048  -1.044  -0.888  -1.180  -1.202  -0.949  -1.116  -1.108  -0.914
29    L        -0.449  -0.580  -0.779  -0.367  -0.556  -0.833  -0.400  -0.561  -0.793
30    K        -0.436  -0.399  -0.384  -0.516  -0.440  -0.434  -0.639  -0.587  -0.577
31    Y         1.191   1.201   1.251   1.191   1.200   1.284   1.268   1.281   1.353
32    I        -0.436  -0.482  -0.428  -0.535  -0.653  -0.571  -0.639  -0.705  -0.639
33    F         0.150   0.199   0.219   0.171   0.252   0.240  -0.162  -0.075  -0.084
34    T         0.068   0.069   0.179  -0.196  -0.214  -0.024  -0.321  -0.325  -0.161
35    T         1.191   1.201   1.251   1.166   1.184   1.261   1.268   1.281   1.353
36    H         1.191   1.201   1.251   1.350   1.365   1.431   1.268   1.281   1.353
37    F         1.191   1.201   1.251   1.153   1.159   1.249   1.268   1.281   1.353
38    W         1.191   1.201   1.251   1.366   1.376   1.446   1.268   1.281   1.353
39    G         1.191   1.201   1.251   1.157   1.167   1.253   1.268   1.281   1.353
40    P         1.191   1.201   1.251   1.236   1.249   1.326   1.268   1.281   1.353
41    V        -0.197  -0.291  -0.365  -0.218  -0.365  -0.529  -0.321  -0.441  -0.553
42    S         1.191   1.201   1.251   1.136   1.141   1.234   1.268   1.281   1.353
43    N         1.191   1.201   1.251   1.248   1.263   1.338   1.268   1.281   1.353
44    F         1.191   1.201   1.251   1.153   1.159   1.249   1.268   1.281   1.353
45    G         1.191   1.201   1.251   1.157   1.167   1.253   1.268   1.281   1.353
46    I         1.191   1.201   1.251   1.207   1.217   1.299   1.268   1.281   1.353
47    P         1.191   1.201   1.251   1.236   1.249   1.326   1.268   1.281   1.353
48    I         0.325   0.325   0.251   0.328   0.315   0.180   0.156   0.152  -0.023
49    A         1.191   1.201   1.251   1.034   1.081   1.139   1.268   1.281   1.353
50    A         1.191   1.201   1.251   1.034   1.081   1.139   1.268   1.281   1.353
51    I        -0.796  -0.756  -0.820  -0.774  -0.751  -0.789  -0.877  -0.839  -0.848
52    Y        -0.544  -0.527  -0.433  -0.743  -0.737  -0.554  -0.798  -0.770  -0.646
53    D         1.191   1.201   1.251   1.301   1.320   1.387   1.268   1.281   1.353
54    L         0.325   0.321   0.256   0.169   0.146   0.047   0.156   0.145  -0.013
55    K         0.325   0.321   0.256   0.231   0.266   0.124   0.156   0.145  -0.013
56    K         1.191   1.201   1.251   1.081   1.136   1.183   1.268   1.281   1.353
57    D         0.325   0.321   0.256   0.406   0.395   0.247   0.156   0.145  -0.013
58    P         0.620   0.650   0.360   0.740   0.778   0.349   0.632   0.674   0.179
59    T        -0.953  -0.978  -0.859  -0.855  -0.873  -0.699  -1.036  -1.046  -0.886
60    L         0.073   0.089  -0.276   0.103   0.108  -0.369   0.076   0.096  -0.424
61    I         1.191   1.201   1.251   1.207   1.217   1.299   1.268   1.281   1.353
62    S         1.191   1.201   1.251   1.136   1.141   1.234   1.268   1.281   1.353
63    G         1.191   1.201   1.251   1.157   1.167   1.253   1.268   1.281   1.353
64    P        -0.449  -0.551  -0.790  -0.161  -0.290  -0.655  -0.400  -0.525  -0.806
65    M         1.191   1.201   1.251   1.301   1.317   1.387   1.268   1.281   1.353
66    T         1.191   1.201   1.251   1.166   1.184   1.261   1.268   1.281   1.353
67    F        -0.436  -0.376  -0.407  -0.583  -0.499  -0.575  -0.639  -0.552  -0.610
68    A         0.150   0.175   0.212  -0.152  -0.091  -0.060  -0.162  -0.120  -0.098
69    L         1.191   1.201   1.251   1.052   1.053   1.155   1.268   1.281   1.353
70    V        -0.796  -0.783  -0.837  -0.736  -0.723  -0.743  -0.877  -0.849  -0.863
71    T        -1.646  -1.596  -1.502  -1.617  -1.579  -1.410  -1.354  -1.311  -1.160
72    Y         1.191   1.201   1.251   1.191   1.200   1.284   1.268   1.281   1.353
73    S         0.620   0.607   0.346   0.640   0.616   0.234   0.632   0.610   0.154
74    G        -1.204  -1.257  -1.207  -1.305  -1.403  -1.307  -1.116  -1.151  -1.045
75    V         0.325   0.321   0.256   0.302   0.282   0.162   0.156   0.145  -0.013
76    F         1.191   1.201   1.251   1.153   1.159   1.249   1.268   1.281   1.353
77    M         1.191   1.201   1.251   1.301   1.317   1.387   1.268   1.281   1.353
78    K         0.150   0.170   0.199   0.089   0.126   0.113  -0.162  -0.129  -0.122
79    Y         1.191   1.201   1.251   1.191   1.200   1.284   1.268   1.281   1.353
80    A         0.068   0.095   0.188  -0.304  -0.243  -0.103  -0.321  -0.272  -0.144
81    L        -0.688  -0.752  -0.819  -0.640  -0.727  -0.775  -0.718  -0.776  -0.840
82    S         0.073   0.011  -0.293   0.093   0.045  -0.392   0.076  -0.013  -0.449
83    V         1.191   1.201   1.251   1.187   1.192   1.280   1.268   1.281   1.353
84    S        -0.197  -0.229  -0.359  -0.169  -0.230  -0.416  -0.321  -0.375  -0.550
85    P         1.191   1.201   1.251   1.236   1.249   1.326   1.268   1.281   1.353
86    K        -0.953  -0.940  -0.848  -0.903  -0.852  -0.717  -1.036  -1.012  -0.876
87    N         1.191   1.201   1.251   1.248   1.263   1.338   1.268   1.281   1.353
88    Y         0.073   0.089  -0.276   0.243   0.253  -0.248   0.076   0.096  -0.424
89    L         1.191   1.201   1.251   1.052   1.053   1.155   1.268   1.281   1.353
90    L         1.191   1.201   1.251   1.052   1.053   1.155   1.268   1.281   1.353
91    F         0.620   0.522   0.336   0.659   0.519   0.236   0.632   0.480   0.135
92    G         0.073   0.116  -0.274   0.166   0.216  -0.374   0.076   0.134  -0.421
93    C         1.191   1.201   1.251   1.378   1.391   1.457   1.268   1.281   1.353
94    H         1.191   1.201   1.251   1.350   1.365   1.431   1.268   1.281   1.353
95    L        -0.688  -0.668  -0.801  -0.704  -0.714  -0.913  -0.718  -0.712  -0.824
96    I        -0.197  -0.244  -0.346  -0.146  -0.219  -0.379  -0.321  -0.370  -0.526
97    N         1.191   1.201   1.251   1.248   1.263   1.338   1.268   1.281   1.353
98    E         0.073  -0.040  -0.298   0.339   0.204  -0.244   0.076  -0.080  -0.455
99    T        -1.394  -1.346  -1.448  -1.148  -1.088  -1.261  -1.116  -1.065  -1.116
100   A         0.620   0.522   0.336   0.557   0.465   0.177   0.632   0.480   0.135
101   Q         1.191   1.201   1.251   1.277   1.314   1.364   1.268   1.281   1.353
102   L         0.620   0.522   0.336   0.571   0.435   0.184   0.632   0.480   0.135
103   A        -1.299  -1.237  -1.197  -1.410  -1.322  -1.240  -1.195  -1.139  -1.037
104   Q         1.191   1.201   1.251   1.277   1.314   1.364   1.268   1.281   1.353
105   G         1.191   1.201   1.251   1.157   1.167   1.253   1.268   1.281   1.353
106   Y        -0.688  -0.752  -0.819  -0.621  -0.710  -0.846  -0.718  -0.776  -0.840
107   R         1.191   1.201   1.251   1.322   1.326   1.405   1.268   1.281   1.353
108   F        -0.544  -0.512  -0.418  -0.631  -0.613  -0.444  -0.798  -0.757  -0.628
109   L        -0.796  -0.760  -0.826  -0.928  -0.906  -0.942  -0.877  -0.844  -0.854
110   K        -0.544  -0.541  -0.441  -0.685  -0.656  -0.510  -0.798  -0.794  -0.657
111   Y        -1.299  -1.282  -1.195  -1.121  -1.127  -0.956  -1.195  -1.172  -1.037
112   T        -1.299  -1.276  -1.200  -0.996  -0.974  -0.872  -1.195  -1.173  -1.041
113   Y         1.191   1.201   1.251   1.191   1.200   1.284   1.268   1.281   1.353
114   F        -0.606  -0.592  -0.446  -0.729  -0.717  -0.502  -0.877  -0.857  -0.663
115   T        -0.228  -0.165  -0.362  -0.236  -0.149  -0.488  -0.341  -0.251  -0.547
116   T        -0.111  -0.010  -0.317  -0.035   0.095  -0.357  -0.177  -0.036  -0.482
117   D        -0.372  -0.368  -0.380  -0.426  -0.408  -0.407  -0.571  -0.550  -0.574
118   E         0.271   0.296   0.240   0.378   0.426   0.297   0.061   0.101  -0.044
119   E        -0.862  -0.859  -0.847  -0.879  -0.857  -0.850  -0.944  -0.937  -0.874
120   K        -1.252  -1.157  -1.188  -1.345  -1.172  -1.217  -1.145  -1.049  -1.027
121   K        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
122   A        -1.646  -1.622  -1.497  -1.748  -1.711  -1.516  -1.354  -1.327  -1.156
123   L        -1.803  -1.709  -1.721  -1.662  -1.486  -1.566  -1.354  -1.270  -1.220
124   D        -0.891  -0.939  -0.850  -0.812  -0.859  -0.764  -0.957  -0.995  -0.875
125   K        -1.143  -1.151  -1.176  -1.167  -1.158  -1.196  -1.036  -1.039  -1.018
126   E        -1.299  -1.305  -1.206  -1.209  -1.247  -1.112  -1.195  -1.196  -1.046
127   W        -1.803  -1.830  -1.721  -1.448  -1.523  -1.398  -1.354  -1.369  -1.220
128   K        -0.891  -0.845  -0.847  -1.020  -0.913  -0.925  -0.957  -0.912  -0.873
129   E        -1.898  -1.832  -1.732  -1.636  -1.550  -1.427  -1.433  -1.381  -1.229
130   K        -0.688  -0.779  -0.815  -0.711  -0.813  -0.903  -0.718  -0.813  -0.838
131   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
132   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
133   E        -1.926  -1.924  -1.747  -1.649  -1.642  -1.378  -1.447  -1.438  -1.237
134   K        -1.252  -1.315  -1.196  -1.300  -1.367  -1.186  -1.145  -1.192  -1.037
135   T        -1.252  -1.221  -1.199  -1.315  -1.269  -1.208  -1.145  -1.103  -1.038
136   G        -0.987  -0.923  -0.869  -1.235  -1.137  -1.040  -1.049  -0.970  -0.894
137   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
138   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
139   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
140   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
141   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
142   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
143   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
144   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
145   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
146   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
147   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
148   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
149   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
150   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
151   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
152   K        -0.987  -0.994  -0.888  -1.216  -1.215  -1.066  -1.049  -1.054  -0.913
153   Q        -0.793  -0.748  -0.816  -0.829  -0.761  -0.859  -0.844  -0.790  -0.840
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P53157
Output conservation file - output/P53157
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
