1     M         1.393   1.406   1.474   1.620   1.637   1.731   1.521   1.539   1.641
2     L         0.758   0.765   0.554   0.630   0.640   0.369   0.776   0.786   0.399
3     K         1.393   1.406   1.474   1.304   1.325   1.430   1.521   1.539   1.641
4     L         0.277   0.257   0.346  -0.088  -0.164  -0.027  -0.075  -0.125  -0.003
5     R         0.277   0.310   0.372   0.303   0.354   0.441  -0.075  -0.019   0.049
6     Q        -0.653  -0.697  -0.704  -0.537  -0.584  -0.627  -0.714  -0.753  -0.741
7     L         0.445   0.499   0.449   0.227   0.304   0.247   0.244   0.338   0.201
8     Q        -0.251  -0.306  -0.249  -0.363  -0.422  -0.339  -0.501  -0.555  -0.469
9     K        -0.126  -0.174  -0.223  -0.242  -0.321  -0.380  -0.288  -0.365  -0.431
10    K        -0.418  -0.482  -0.640  -0.353  -0.442  -0.669  -0.395  -0.479  -0.663
11    K        -0.126  -0.081  -0.194  -0.219  -0.144  -0.312  -0.288  -0.217  -0.382
12    Q         0.241   0.242   0.343   0.130   0.121   0.276  -0.147  -0.154  -0.007
13    K        -0.835  -0.796  -0.744  -0.922  -0.874  -0.786  -0.927  -0.883  -0.789
14    E        -0.653  -0.720  -0.706  -0.683  -0.771  -0.753  -0.714  -0.782  -0.745
15    N        -1.420  -1.474  -1.407  -1.372  -1.501  -1.433  -1.140  -1.183  -1.074
16    E        -0.219  -0.174  -0.218  -0.298  -0.223  -0.312  -0.425  -0.353  -0.421
17    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
18    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
19    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
20    -        -0.210  -0.053  -0.208  -0.220   0.031  -0.255  -0.386  -0.158  -0.400
21    -        -0.387  -0.412  -0.291  -0.473  -0.499  -0.321  -0.659  -0.686  -0.522
22    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
23    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
24    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
25    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
26    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
27    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
28    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
29    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
30    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
31    N        -0.850  -0.877  -0.753  -0.993  -1.014  -0.833  -0.939  -0.958  -0.797
32    S        -1.240  -1.183  -1.118  -1.226  -1.108  -1.054  -1.128  -1.070  -0.967
33    S        -1.222  -1.213  -1.123  -1.272  -1.258  -1.147  -1.120  -1.107  -0.973
34    S        -0.761  -0.742  -0.730  -0.666  -0.634  -0.629  -0.842  -0.817  -0.773
35    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
36    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
37    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
38    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
39    I        -1.531  -1.523  -1.422  -1.519  -1.487  -1.353  -1.246  -1.230  -1.088
40    Q        -1.823  -1.805  -1.692  -1.717  -1.677  -1.499  -1.352  -1.331  -1.180
41    P        -1.238  -1.228  -1.121  -1.312  -1.271  -1.122  -1.140  -1.119  -0.972
42    N        -1.531  -1.558  -1.434  -1.482  -1.516  -1.332  -1.246  -1.255  -1.096
43    L        -0.653  -0.706  -0.720  -0.651  -0.760  -0.790  -0.714  -0.781  -0.764
44    S        -0.126  -0.174  -0.202  -0.082  -0.140  -0.217  -0.288  -0.341  -0.394
45    A         0.224   0.245   0.346  -0.010   0.022   0.183  -0.182  -0.149  -0.003
46    A         0.758   0.652   0.539   0.900   0.765   0.579   0.776   0.603   0.371
47    R        -0.418  -0.535  -0.671  -0.208  -0.401  -0.675  -0.395  -0.542  -0.699
48    I         0.224   0.245   0.346  -0.241  -0.200  -0.026  -0.182  -0.149  -0.002
49    R         1.393   1.406   1.474   1.526   1.543   1.641   1.521   1.539   1.641
50    L        -0.251  -0.217  -0.232  -0.565  -0.517  -0.506  -0.501  -0.445  -0.446
51    K         0.445   0.382   0.391   0.452   0.331   0.285   0.244   0.120   0.088
52    R         0.758   0.748   0.548   0.749   0.742   0.470   0.776   0.759   0.389
53    D         1.393   1.406   1.474   1.436   1.449   1.556   1.521   1.539   1.641
54    L        -0.251  -0.219  -0.231  -0.605  -0.559  -0.528  -0.501  -0.450  -0.445
55    D        -0.835  -0.835  -0.756  -0.852  -0.856  -0.729  -0.927  -0.922  -0.802
56    S        -0.418  -0.521  -0.658  -0.285  -0.444  -0.675  -0.395  -0.525  -0.684
57    L         0.445   0.431   0.403   0.142   0.111   0.055   0.244   0.213   0.112
58    D        -0.361  -0.329  -0.281  -0.496  -0.445  -0.390  -0.608  -0.563  -0.505
59    L         0.758   0.765   0.554   0.607   0.616   0.319   0.776   0.786   0.399
60    P         0.152   0.098  -0.120   0.287   0.209  -0.110   0.137   0.052  -0.270
61    P        -0.653  -0.673  -0.709  -0.610  -0.653  -0.697  -0.714  -0.744  -0.750
62    T        -0.126  -0.144  -0.195   0.023   0.026  -0.090  -0.288  -0.294  -0.384
63    V        -0.433  -0.423  -0.313  -0.528  -0.522  -0.354  -0.714  -0.699  -0.550
64    T        -1.128  -1.180  -1.104  -1.037  -1.075  -0.938  -1.033  -1.074  -0.957
65    L        -0.835  -0.862  -0.758  -0.933  -0.966  -0.839  -0.927  -0.942  -0.804
66    N        -1.531  -1.516  -1.425  -1.315  -1.294  -1.146  -1.246  -1.225  -1.090
67    V        -0.946  -1.043  -1.082  -0.612  -0.751  -0.817  -0.820  -0.924  -0.933
68    I        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
69    T        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
70    S        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
71    P        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
72    D        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
73    S        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
74    A        -0.126  -0.190  -0.239  -0.241  -0.383  -0.471  -0.288  -0.393  -0.453
75    D         0.152   0.235  -0.092   0.293   0.402  -0.084   0.137   0.252  -0.229
76    R        -0.946  -1.023  -1.073  -0.799  -0.927  -1.015  -0.820  -0.903  -0.924
77    S        -1.531  -1.491  -1.412  -1.466  -1.406  -1.277  -1.246  -1.205  -1.080
78    Q        -0.219  -0.278  -0.264  -0.307  -0.412  -0.399  -0.425  -0.508  -0.486
79    S        -1.128  -1.180  -1.110  -1.344  -1.415  -1.274  -1.033  -1.072  -0.961
80    P        -1.128  -1.064  -1.069  -0.988  -0.868  -0.942  -1.033  -0.957  -0.922
81    K        -0.946  -1.043  -1.082  -0.868  -1.006  -1.079  -0.820  -0.924  -0.933
82    L        -0.251  -0.318  -0.253  -0.443  -0.549  -0.450  -0.501  -0.575  -0.476
83    E        -1.128  -1.035  -1.076  -1.177  -1.038  -1.083  -1.033  -0.941  -0.932
84    V         0.152   0.124  -0.127   0.050   0.010  -0.348   0.137   0.096  -0.280
85    I        -1.823  -1.763  -1.681  -1.565  -1.496  -1.347  -1.352  -1.302  -1.173
86    V        -0.126  -0.069  -0.199  -0.216  -0.138  -0.361  -0.288  -0.216  -0.395
87    R        -1.128  -1.093  -1.079  -1.046  -1.023  -0.983  -1.033  -0.999  -0.934
88    P         1.393   1.406   1.474   1.406   1.423   1.527   1.521   1.539   1.641
89    D         0.445   0.537   0.457   0.436   0.575   0.439   0.244   0.404   0.217
90    E         0.152   0.101  -0.120   0.276   0.194  -0.136   0.137   0.057  -0.270
91    G         1.393   1.406   1.474   1.479   1.496   1.597   1.521   1.539   1.641
92    Y        -0.126  -0.073  -0.211   0.081   0.151  -0.055  -0.288  -0.221  -0.413
93    Y         1.393   1.406   1.474   1.588   1.605   1.700   1.521   1.539   1.641
94    N        -0.418  -0.496  -0.649  -0.369  -0.481  -0.711  -0.395  -0.496  -0.674
95    Y         0.152   0.190  -0.106   0.358   0.410  -0.026   0.137   0.188  -0.249
96    G         1.393   1.406   1.474   1.479   1.496   1.597   1.521   1.539   1.641
97    S        -0.653  -0.651  -0.694  -0.686  -0.679  -0.722  -0.714  -0.714  -0.734
98    I         0.152   0.038  -0.143   0.401   0.253  -0.042   0.137  -0.029  -0.304
99    N        -1.531  -1.534  -1.417  -1.429  -1.414  -1.261  -1.246  -1.239  -1.084
100   F         1.393   1.406   1.474   1.516   1.540   1.632   1.521   1.539   1.641
101   N        -1.238  -1.197  -1.120  -1.285  -1.218  -1.077  -1.140  -1.095  -0.972
102   L        -1.531  -1.528  -1.424  -1.453  -1.466  -1.304  -1.246  -1.235  -1.089
103   D        -1.823  -1.826  -1.692  -1.557  -1.546  -1.355  -1.352  -1.345  -1.179
104   F        -0.126  -0.110  -0.211  -0.144  -0.132  -0.266  -0.288  -0.277  -0.411
105   N        -0.946  -0.998  -1.060  -0.931  -1.038  -1.144  -0.820  -0.879  -0.911
106   E        -1.238  -1.159  -1.120  -1.318  -1.206  -1.132  -1.140  -1.069  -0.972
107   V        -0.126  -0.190  -0.239  -0.165  -0.261  -0.314  -0.288  -0.393  -0.453
108   Y         0.224   0.252   0.352   0.156   0.213   0.360  -0.182  -0.135   0.009
109   P         1.393   1.406   1.474   1.406   1.423   1.527   1.521   1.539   1.641
110   I        -0.251  -0.306  -0.249  -0.245  -0.306  -0.194  -0.501  -0.555  -0.469
111   E         0.277   0.297   0.359   0.075   0.103   0.192  -0.075  -0.045   0.025
112   P         1.393   1.406   1.474   1.406   1.423   1.527   1.521   1.539   1.641
113   P         1.393   1.406   1.474   1.406   1.423   1.527   1.521   1.539   1.641
114   K         0.758   0.652   0.539   0.740   0.604   0.435   0.776   0.603   0.371
115   V         0.445   0.537   0.457   0.416   0.568   0.405   0.244   0.404   0.217
116   V        -0.418  -0.404  -0.631  -0.360  -0.341  -0.668  -0.395  -0.377  -0.652
117   C         1.393   1.406   1.474   1.652   1.668   1.761   1.521   1.539   1.641
118   L        -0.418  -0.482  -0.640  -0.455  -0.541  -0.740  -0.395  -0.479  -0.663
119   K        -1.128  -1.143  -1.088  -1.101  -1.101  -1.022  -1.033  -1.039  -0.943
120   K         0.758   0.652   0.539   0.749   0.616   0.455   0.776   0.603   0.371
121   I         0.758   0.797   0.559   0.812   0.858   0.534   0.776   0.834   0.409
122   F         0.445   0.433   0.407   0.577   0.556   0.473   0.244   0.216   0.120
123   H         1.393   1.406   1.474   1.645   1.661   1.755   1.521   1.539   1.641
124   P         1.393   1.406   1.474   1.406   1.423   1.527   1.521   1.539   1.641
125   N         1.393   1.406   1.474   1.331   1.354   1.456   1.521   1.539   1.641
126   I         1.393   1.406   1.474   1.376   1.389   1.498   1.521   1.539   1.641
127   D         1.393   1.406   1.474   1.436   1.449   1.556   1.521   1.539   1.641
128   L        -0.126  -0.185  -0.229  -0.395  -0.503  -0.546  -0.288  -0.384  -0.439
129   K        -1.128  -1.117  -1.101  -1.055  -1.048  -0.993  -1.033  -1.020  -0.955
130   G         1.393   1.406   1.474   1.479   1.496   1.597   1.521   1.539   1.641
131   N         0.445   0.470   0.409   0.262   0.306   0.158   0.244   0.284   0.124
132   V         0.277   0.322   0.373   0.094   0.178   0.232  -0.075   0.005   0.053
133   C         1.393   1.406   1.474   1.652   1.668   1.761   1.521   1.539   1.641
134   L         1.393   1.406   1.474   1.185   1.201   1.317   1.521   1.539   1.641
135   N         1.393   1.406   1.474   1.331   1.354   1.456   1.521   1.539   1.641
136   I         0.758   0.652   0.539   0.772   0.613   0.410   0.776   0.603   0.371
137   L         1.393   1.406   1.474   1.185   1.201   1.317   1.521   1.539   1.641
138   R         1.393   1.406   1.474   1.526   1.543   1.641   1.521   1.539   1.641
139   E         0.758   0.765   0.554   0.854   0.866   0.575   0.776   0.786   0.399
140   D         1.393   1.406   1.474   1.436   1.449   1.556   1.521   1.539   1.641
141   W         1.393   1.406   1.474   1.677   1.694   1.785   1.521   1.539   1.641
142   S        -0.653  -0.697  -0.704  -0.663  -0.695  -0.742  -0.714  -0.753  -0.741
143   P         1.393   1.406   1.474   1.406   1.423   1.527   1.521   1.539   1.641
144   A         0.277   0.257   0.346   0.141   0.094   0.226  -0.075  -0.125  -0.003
145   L         1.393   1.406   1.474   1.185   1.201   1.317   1.521   1.539   1.641
146   D        -0.433  -0.388  -0.307  -0.690  -0.606  -0.485  -0.714  -0.654  -0.542
147   L         0.758   0.652   0.539   0.607   0.461   0.294   0.776   0.603   0.371
148   Q        -0.251  -0.216  -0.247  -0.365  -0.313  -0.305  -0.501  -0.471  -0.470
149   S        -0.653  -0.592  -0.678  -0.539  -0.431  -0.545  -0.714  -0.634  -0.711
150   I         0.277   0.327   0.377   0.062   0.144   0.215  -0.075   0.014   0.060
151   I        -1.128  -1.060  -1.068  -1.186  -1.079  -1.055  -1.033  -0.958  -0.924
152   T        -1.128  -1.099  -1.081  -1.093  -1.064  -1.007  -1.033  -0.998  -0.935
153   G         1.393   1.406   1.474   1.479   1.496   1.597   1.521   1.539   1.641
154   L         0.758   0.652   0.539   0.607   0.461   0.294   0.776   0.603   0.371
155   L        -0.433  -0.375  -0.297  -0.782  -0.705  -0.581  -0.714  -0.639  -0.529
156   F         0.152   0.168  -0.108   0.414   0.442   0.040   0.137   0.158  -0.252
157   L         1.393   1.406   1.474   1.185   1.201   1.317   1.521   1.539   1.641
158   F         1.393   1.406   1.474   1.516   1.540   1.632   1.521   1.539   1.641
159   L         0.152   0.037  -0.137   0.109  -0.035  -0.243   0.137  -0.030  -0.295
160   E         0.152   0.206  -0.099   0.283   0.355  -0.086   0.137   0.211  -0.239
161   P         1.393   1.406   1.474   1.406   1.423   1.527   1.521   1.539   1.641
162   N         1.393   1.406   1.474   1.331   1.354   1.456   1.521   1.539   1.641
163   P        -0.361  -0.369  -0.302  -0.460  -0.480  -0.392  -0.608  -0.624  -0.534
164   N        -1.128  -1.133  -1.091  -1.103  -1.096  -1.013  -1.033  -1.030  -0.944
165   D         1.393   1.406   1.474   1.436   1.449   1.556   1.521   1.539   1.641
166   P         1.393   1.406   1.474   1.406   1.423   1.527   1.521   1.539   1.641
167   L         1.393   1.406   1.474   1.185   1.201   1.317   1.521   1.539   1.641
168   N         1.393   1.406   1.474   1.331   1.354   1.456   1.521   1.539   1.641
169   K         1.393   1.406   1.474   1.304   1.325   1.430   1.521   1.539   1.641
170   D         0.277   0.296   0.358   0.085   0.110   0.195  -0.075  -0.047   0.022
171   A         1.393   1.406   1.474   1.466   1.487   1.585   1.521   1.539   1.641
172   A         1.393   1.406   1.474   1.466   1.487   1.585   1.521   1.539   1.641
173   K        -1.238  -1.197  -1.120  -1.342  -1.286  -1.122  -1.140  -1.095  -0.972
174   L        -1.128  -1.161  -1.101  -1.062  -1.108  -1.027  -1.033  -1.057  -0.954
175   L         0.758   0.797   0.559   0.645   0.692   0.409   0.776   0.834   0.409
176   C        -1.823  -1.826  -1.692  -1.468  -1.490  -1.273  -1.352  -1.345  -1.179
177   E        -0.653  -0.632  -0.705  -0.749  -0.737  -0.806  -0.714  -0.707  -0.746
178   G        -0.251  -0.262  -0.249  -0.441  -0.454  -0.394  -0.501  -0.518  -0.471
179   E        -0.835  -0.837  -0.756  -0.939  -0.950  -0.836  -0.927  -0.924  -0.802
180   K        -1.238  -1.178  -1.119  -1.479  -1.414  -1.271  -1.140  -1.082  -0.971
181   E        -1.128  -1.088  -1.080  -1.022  -0.969  -0.929  -1.033  -0.993  -0.935
182   F         1.393   1.406   1.474   1.516   1.540   1.632   1.521   1.539   1.641
183   A        -1.238  -1.189  -1.118  -1.280  -1.214  -1.063  -1.140  -1.092  -0.970
184   E        -1.238  -1.197  -1.122  -1.281  -1.228  -1.112  -1.140  -1.095  -0.973
185   A        -0.946  -1.043  -1.082  -0.934  -1.103  -1.188  -0.820  -0.924  -0.933
186   V         1.393   1.406   1.474   1.466   1.485   1.585   1.521   1.539   1.641
187   R        -0.653  -0.644  -0.686  -0.608  -0.585  -0.680  -0.714  -0.693  -0.723
188   L        -1.238  -1.162  -1.117  -1.220  -1.116  -1.033  -1.140  -1.073  -0.969
189   T        -0.251  -0.248  -0.241  -0.304  -0.283  -0.254  -0.501  -0.492  -0.461
190   M         0.758   0.652   0.539   1.017   0.861   0.638   0.776   0.603   0.371
191   S        -0.725  -0.695  -0.706  -0.716  -0.665  -0.683  -0.820  -0.785  -0.750
192   G         1.393   1.406   1.474   1.479   1.496   1.597   1.521   1.539   1.641
193   G         0.152   0.037  -0.137   0.338   0.169  -0.118   0.137  -0.030  -0.295
194   S        -1.531  -1.535  -1.420  -1.348  -1.373  -1.205  -1.246  -1.239  -1.086
195   I        -0.251  -0.209  -0.241  -0.426  -0.378  -0.429  -0.501  -0.458  -0.462
196   E        -1.420  -1.390  -1.397  -1.314  -1.272  -1.320  -1.140  -1.107  -1.067
197   H        -0.653  -0.583  -0.690  -0.555  -0.445  -0.607  -0.714  -0.632  -0.728
198   V        -1.128  -1.072  -1.079  -1.026  -0.932  -0.940  -1.033  -0.967  -0.934
199   K        -1.823  -1.790  -1.678  -1.362  -1.318  -1.168  -1.352  -1.321  -1.171
200   Y         0.445   0.470   0.421   0.577   0.618   0.499   0.244   0.284   0.146
201   D         0.445   0.470   0.409   0.398   0.432   0.299   0.244   0.284   0.124
202   N        -0.835  -0.880  -0.756  -0.825  -0.905  -0.718  -0.927  -0.963  -0.802
203   I         0.445   0.382   0.391   0.416   0.296   0.270   0.244   0.120   0.088
204   V        -0.725  -0.691  -0.700  -0.803  -0.758  -0.749  -0.820  -0.778  -0.745
205   S        -1.322  -1.286  -1.152  -1.232  -1.173  -0.983  -1.203  -1.165  -0.997
206   P        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P52491
Output conservation file - output/P52491
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
