1     M         1.159   1.182   1.239   1.494   1.522   1.579   1.224   1.256   1.343
2     S         0.447   0.313   0.177   0.722   0.541   0.254   0.448   0.244  -0.015
3     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
4     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
5     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
6     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
7     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
8     I         0.308   0.197   0.107   0.356   0.149  -0.083   0.239   0.056  -0.139
9     A        -2.517  -2.410  -2.142  -2.170  -2.023  -1.665  -1.815  -1.713  -1.424
10    S        -1.769  -1.750  -1.541  -1.691  -1.652  -1.373  -1.526  -1.485  -1.227
11    Y        -0.738  -0.806  -0.641  -0.917  -1.004  -0.708  -0.947  -0.998  -0.774
12    A        -0.345  -0.524  -0.597  -0.299  -0.555  -0.665  -0.368  -0.611  -0.713
13    Q        -1.253  -1.263  -1.142  -1.192  -1.178  -0.968  -1.236  -1.237  -1.052
14    E        -0.345  -0.420  -0.580  -0.224  -0.299  -0.556  -0.368  -0.483  -0.691
15    L         0.384   0.447   0.163   0.155   0.233  -0.141   0.356   0.447  -0.039
16    K         0.384   0.447   0.163   0.437   0.545   0.141   0.356   0.447  -0.039
17    L        -1.021  -1.045  -1.109  -1.064  -1.071  -1.253  -0.947  -0.982  -1.016
18    A        -1.132  -1.022  -1.113  -1.162  -1.034  -1.067  -1.103  -0.997  -1.024
19    L         1.159   1.182   1.239   0.876   0.908   1.031   1.224   1.256   1.343
20    H        -1.336  -1.276  -1.132  -1.168  -1.066  -0.894  -1.325  -1.226  -1.041
21    Q         0.447   0.313   0.177   0.722   0.551   0.319   0.448   0.244  -0.015
22    Y        -0.091  -0.063   0.049  -0.122  -0.084   0.048  -0.438  -0.397  -0.245
23    P         0.447   0.485   0.179   0.669   0.715   0.270   0.448   0.502  -0.011
24    N        -0.091  -0.110   0.035  -0.059  -0.144   0.075  -0.438  -0.485  -0.270
25    F         1.159   1.182   1.239   1.153   1.196   1.276   1.224   1.256   1.343
26    P         1.159   1.182   1.239   1.291   1.320   1.399   1.224   1.256   1.343
27    S        -1.132  -1.210  -1.116  -0.967  -1.102  -0.991  -1.103  -1.174  -1.027
28    E        -0.542  -0.692  -0.622  -0.608  -0.810  -0.744  -0.660  -0.848  -0.751
29    G         1.159   1.182   1.239   1.088   1.116   1.219   1.224   1.256   1.343
30    I         1.159   1.182   1.239   1.232   1.253   1.346   1.224   1.256   1.343
31    L        -0.231  -0.335  -0.536  -0.355  -0.488  -0.717  -0.216  -0.358  -0.632
32    F         1.159   1.182   1.239   1.153   1.196   1.276   1.224   1.256   1.343
33    E         0.447   0.313   0.177   0.526   0.378   0.122   0.448   0.244  -0.015
34    D         1.159   1.182   1.239   1.258   1.283   1.369   1.224   1.256   1.343
35    F        -0.091  -0.110   0.035  -0.338  -0.392  -0.141  -0.438  -0.485  -0.270
36    L         0.447   0.323   0.159   0.278   0.128  -0.041   0.448   0.259  -0.046
37    P         1.159   1.182   1.239   1.291   1.320   1.399   1.224   1.256   1.343
38    I        -0.231  -0.283  -0.534  -0.095  -0.222  -0.641  -0.216  -0.295  -0.629
39    F         1.159   1.182   1.239   1.153   1.196   1.276   1.224   1.256   1.343
40    R        -0.885  -0.773  -0.654  -0.816  -0.667  -0.489  -1.103  -0.952  -0.789
41    N        -1.132  -0.864  -1.086  -1.065  -0.663  -0.985  -1.103  -0.788  -0.994
42    P         0.447   0.313   0.177   0.651   0.461   0.221   0.448   0.244  -0.015
43    G        -1.787  -1.721  -1.541  -1.746  -1.638  -1.379  -1.546  -1.465  -1.227
44    L        -0.869  -1.066  -1.103  -0.972  -1.263  -1.231  -0.771  -0.996  -1.010
45    F         0.447   0.313   0.177   0.557   0.405   0.217   0.448   0.244  -0.015
46    Q        -1.460  -1.546  -1.503  -1.156  -1.343  -1.312  -1.214  -1.296  -1.194
47    K        -0.869  -1.066  -1.103  -0.752  -1.044  -1.123  -0.771  -0.996  -1.010
48    L         1.159   1.182   1.239   0.876   0.908   1.031   1.224   1.256   1.343
49    I        -0.682  -0.704  -0.608  -0.870  -0.917  -0.780  -0.881  -0.867  -0.734
50    D        -1.460  -1.400  -1.486  -1.210  -1.154  -1.302  -1.214  -1.150  -1.179
51    A        -0.231  -0.324  -0.559  -0.204  -0.353  -0.736  -0.216  -0.343  -0.663
52    F         0.096  -0.026   0.057  -0.114  -0.396  -0.297  -0.106  -0.330  -0.229
53    K        -0.542  -0.671  -0.613  -0.635  -0.837  -0.791  -0.660  -0.819  -0.740
54    L        -0.231  -0.246  -0.548  -0.356  -0.398  -0.735  -0.216  -0.248  -0.649
55    H        -0.231  -0.477  -0.567   0.241  -0.127  -0.362  -0.216  -0.541  -0.673
56    L        -0.885  -0.861  -0.664  -1.339  -1.289  -0.936  -1.103  -1.061  -0.802
57    E        -1.132  -1.007  -1.100  -1.206  -0.957  -1.149  -1.103  -0.938  -1.008
58    E        -0.345  -0.524  -0.597  -0.236  -0.467  -0.605  -0.368  -0.611  -0.713
59    A        -1.358  -1.305  -1.148  -1.426  -1.351  -1.167  -1.329  -1.266  -1.057
60    F         0.308   0.219   0.144   0.281   0.125  -0.066   0.239   0.092  -0.071
61    P        -1.358  -1.325  -1.157  -1.254  -1.237  -1.004  -1.329  -1.286  -1.065
62    E        -1.358  -1.365  -1.164  -1.402  -1.373  -1.099  -1.329  -1.323  -1.073
63    V        -1.213  -1.185  -1.121  -1.182  -1.104  -0.999  -1.214  -1.169  -1.033
64    K         0.447   0.323   0.159   0.529   0.406   0.152   0.448   0.259  -0.046
65    I        -0.542  -0.555  -0.585  -0.513  -0.524  -0.590  -0.660  -0.649  -0.701
66    D         1.159   1.182   1.239   1.258   1.283   1.369   1.224   1.256   1.343
67    Y        -0.091  -0.110   0.035  -0.091  -0.185   0.041  -0.438  -0.485  -0.270
68    I        -0.091  -0.004   0.055  -0.278  -0.140  -0.059  -0.438  -0.290  -0.232
69    V         0.096   0.190   0.087   0.059   0.194  -0.002  -0.106   0.045  -0.176
70    G         1.159   1.182   1.239   1.088   1.116   1.219   1.224   1.256   1.343
71    L         1.159   1.182   1.239   0.876   0.908   1.031   1.224   1.256   1.343
72    E         0.096   0.043   0.079   0.020  -0.053  -0.042  -0.106  -0.207  -0.190
73    S        -0.151  -0.081   0.046  -0.380  -0.279  -0.078  -0.549  -0.430  -0.249
74    R         1.159   1.182   1.239   1.434   1.454   1.525   1.224   1.256   1.343
75    G         1.159   1.182   1.239   1.088   1.116   1.219   1.224   1.256   1.343
76    F         1.159   1.182   1.239   1.153   1.196   1.276   1.224   1.256   1.343
77    L         1.159   1.182   1.239   0.876   0.908   1.031   1.224   1.256   1.343
78    F         1.159   1.182   1.239   1.153   1.196   1.276   1.224   1.256   1.343
79    G         1.159   1.182   1.239   1.088   1.116   1.219   1.224   1.256   1.343
80    P         1.159   1.182   1.239   1.291   1.320   1.399   1.224   1.256   1.343
81    T        -0.542  -0.532  -0.596  -0.356  -0.364  -0.498  -0.660  -0.652  -0.717
82    L         1.159   1.182   1.239   0.876   0.908   1.031   1.224   1.256   1.343
83    A         1.159   1.182   1.239   1.095   1.143   1.226   1.224   1.256   1.343
84    L         1.159   1.182   1.239   0.876   0.908   1.031   1.224   1.256   1.343
85    A         0.096  -0.026   0.057   0.013  -0.175  -0.035  -0.106  -0.330  -0.229
86    L        -1.132  -1.208  -1.122  -1.310  -1.413  -1.205  -1.103  -1.172  -1.033
87    G        -0.542  -0.529  -0.608  -0.561  -0.554  -0.615  -0.660  -0.648  -0.734
88    V        -0.869  -0.786  -1.074  -0.723  -0.598  -0.984  -0.771  -0.682  -0.980
89    G        -0.542  -0.692  -0.622  -0.678  -0.911  -0.782  -0.660  -0.848  -0.751
90    F         1.159   1.182   1.239   1.153   1.196   1.276   1.224   1.256   1.343
91    V         1.159   1.182   1.239   1.213   1.239   1.330   1.224   1.256   1.343
92    P         1.159   1.182   1.239   1.291   1.320   1.399   1.224   1.256   1.343
93    V         1.159   1.182   1.239   1.213   1.239   1.330   1.224   1.256   1.343
94    R         1.159   1.182   1.239   1.434   1.454   1.525   1.224   1.256   1.343
95    K         1.159   1.182   1.239   1.134   1.197   1.259   1.224   1.256   1.343
96    A        -0.885  -0.838  -0.664  -1.072  -0.967  -0.711  -1.103  -1.031  -0.802
97    G         0.447   0.323   0.159   0.483   0.326   0.115   0.448   0.259  -0.046
98    K         1.159   1.182   1.239   1.134   1.197   1.259   1.224   1.256   1.343
99    L         0.447   0.313   0.177   0.278   0.116  -0.017   0.448   0.244  -0.015
100   P         0.447   0.488   0.174   0.634   0.688   0.176   0.448   0.507  -0.018
101   G         1.159   1.182   1.239   1.088   1.116   1.219   1.224   1.256   1.343
102   E        -1.213  -1.234  -1.129  -1.263  -1.255  -1.118  -1.214  -1.207  -1.040
103   C        -1.336  -1.329  -1.146  -1.196  -1.226  -0.999  -1.325  -1.286  -1.057
104   F        -0.091   0.029   0.065  -0.327  -0.108  -0.084  -0.438  -0.231  -0.215
105   K        -1.336  -1.371  -1.150  -1.271  -1.294  -0.968  -1.325  -1.331  -1.061
106   A         0.096   0.086   0.061  -0.061  -0.080  -0.150  -0.106  -0.132  -0.223
107   T        -1.664  -1.691  -1.527  -1.613  -1.643  -1.384  -1.435  -1.437  -1.216
108   Y        -0.091  -0.005   0.056  -0.122   0.044   0.068  -0.438  -0.292  -0.231
109   E        -1.213  -1.236  -1.126  -1.189  -1.174  -1.031  -1.214  -1.209  -1.037
110   K         1.159   1.182   1.239   1.134   1.197   1.259   1.224   1.256   1.343
111   E         1.159   1.182   1.239   1.164   1.233   1.286   1.224   1.256   1.343
112   Y         1.159   1.182   1.239   1.444   1.476   1.535   1.224   1.256   1.343
113   G         0.447   0.323   0.159   0.472   0.311   0.085   0.448   0.259  -0.046
114   S        -1.132  -1.150  -1.130  -0.923  -0.976  -0.989  -1.103  -1.131  -1.041
115   D         1.159   1.182   1.239   1.258   1.283   1.369   1.224   1.256   1.343
116   L        -2.114  -2.077  -1.855  -2.195  -2.080  -1.828  -1.657  -1.601  -1.334
117   F         1.159   1.182   1.239   1.153   1.196   1.276   1.224   1.256   1.343
118   E        -0.542  -0.671  -0.613  -0.566  -0.739  -0.692  -0.660  -0.819  -0.740
119   I         0.096   0.082   0.074   0.163   0.124   0.119  -0.106  -0.138  -0.198
120   Q         1.159   1.182   1.239   1.339   1.373   1.441   1.224   1.256   1.343
121   K        -1.460  -1.442  -1.484  -1.352  -1.315  -1.419  -1.214  -1.190  -1.178
122   N        -1.132  -1.031  -1.117  -1.005  -0.989  -0.946  -1.103  -1.027  -1.028
123   A        -1.132  -1.124  -1.113  -1.146  -1.106  -1.052  -1.103  -1.067  -1.021
124   I         1.159   1.182   1.239   1.232   1.253   1.346   1.224   1.256   1.343
125   P        -0.682  -0.609  -0.623  -0.811  -0.726  -0.722  -0.881  -0.802  -0.755
126   A        -1.336  -1.144  -1.136  -1.493  -1.138  -1.194  -1.325  -1.105  -1.047
127   G         0.447   0.488   0.174   0.493   0.541   0.162   0.448   0.507  -0.018
128   S        -0.885  -0.782  -0.659  -1.031  -0.829  -0.665  -1.103  -0.961  -0.795
129   N        -1.213  -1.234  -1.129  -1.068  -1.081  -0.905  -1.214  -1.207  -1.040
130   V         1.159   1.182   1.239   1.213   1.239   1.330   1.224   1.256   1.343
131   I        -0.805  -0.676  -0.644  -1.251  -1.066  -0.952  -0.992  -0.812  -0.776
132   I         0.447   0.485   0.179   0.591   0.629   0.176   0.448   0.502  -0.011
133   V         1.159   1.182   1.239   1.213   1.239   1.330   1.224   1.256   1.343
134   D         1.159   1.182   1.239   1.258   1.283   1.369   1.224   1.256   1.343
135   D         1.159   1.182   1.239   1.258   1.283   1.369   1.224   1.256   1.343
136   I         1.159   1.182   1.239   1.232   1.253   1.346   1.224   1.256   1.343
137   I        -0.091  -0.060   0.041  -0.455  -0.428  -0.286  -0.438  -0.392  -0.259
138   A         1.159   1.182   1.239   1.095   1.143   1.226   1.224   1.256   1.343
139   T         1.159   1.182   1.239   1.372   1.391   1.471   1.224   1.256   1.343
140   G         1.159   1.182   1.239   1.088   1.116   1.219   1.224   1.256   1.343
141   G         1.159   1.182   1.239   1.088   1.116   1.219   1.224   1.256   1.343
142   S         0.447   0.323   0.159   0.761   0.604   0.319   0.448   0.259  -0.046
143   A         1.159   1.182   1.239   1.095   1.143   1.226   1.224   1.256   1.343
144   A        -1.991  -1.859  -1.832  -2.083  -1.915  -1.899  -1.546  -1.419  -1.317
145   A        -0.869  -0.683  -1.057  -0.718  -0.460  -0.953  -0.771  -0.547  -0.961
146   A         1.159   1.182   1.239   1.095   1.143   1.226   1.224   1.256   1.343
147   G        -0.231  -0.246  -0.548  -0.186  -0.220  -0.663  -0.216  -0.248  -0.649
148   E         0.096   0.141   0.078   0.020   0.118  -0.044  -0.106  -0.037  -0.191
149   L         1.159   1.182   1.239   0.876   0.908   1.031   1.224   1.256   1.343
150   V        -0.542  -0.365  -0.584  -0.587  -0.330  -0.679  -0.660  -0.426  -0.700
151   E        -1.132  -1.055  -1.115  -1.200  -1.081  -1.211  -1.103  -1.030  -1.026
152   Q        -0.682  -0.716  -0.616  -0.758  -0.756  -0.593  -0.881  -0.887  -0.745
153   L        -0.542  -0.334  -0.570  -0.801  -0.525  -0.782  -0.660  -0.378  -0.679
154   E        -0.869  -0.724  -1.064  -0.779  -0.578  -1.063  -0.771  -0.595  -0.968
155   A        -0.151  -0.090   0.040  -0.598  -0.489  -0.263  -0.549  -0.448  -0.261
156   N        -0.682  -0.545  -0.607  -0.697  -0.486  -0.565  -0.881  -0.696  -0.732
157   L        -1.336  -1.359  -1.148  -1.563  -1.624  -1.266  -1.325  -1.318  -1.059
158   L        -0.682  -0.750  -0.622  -1.096  -1.180  -0.909  -0.881  -0.936  -0.754
159   E         0.096  -0.026   0.057  -0.035  -0.260  -0.173  -0.106  -0.330  -0.229
160   Y        -0.542  -0.617  -0.612  -0.294  -0.385  -0.441  -0.660  -0.739  -0.737
161   N        -1.460  -1.245  -1.483  -1.439  -1.181  -1.354  -1.214  -0.982  -1.177
162   F         1.159   1.182   1.239   1.153   1.196   1.276   1.224   1.256   1.343
163   V        -0.542  -0.688  -0.622  -0.601  -0.891  -0.814  -0.660  -0.845  -0.750
164   M        -0.091  -0.110   0.035  -0.213  -0.363  -0.126  -0.438  -0.485  -0.270
165   E         1.159   1.182   1.239   1.164   1.233   1.286   1.224   1.256   1.343
166   L         0.447   0.313   0.177   0.237   0.053  -0.120   0.448   0.244  -0.015
167   D        -0.869  -1.066  -1.103  -0.690  -1.019  -1.159  -0.771  -0.996  -1.010
168   F         1.159   1.182   1.239   1.153   1.196   1.276   1.224   1.256   1.343
169   L         1.159   1.182   1.239   0.876   0.908   1.031   1.224   1.256   1.343
170   K        -0.231  -0.275  -0.549  -0.076  -0.116  -0.489  -0.216  -0.283  -0.650
171   G         1.159   1.182   1.239   1.088   1.116   1.219   1.224   1.256   1.343
172   R         0.447   0.488   0.174   0.814   0.854   0.390   0.448   0.507  -0.018
173   S        -1.460  -1.540  -1.503  -1.193  -1.360  -1.304  -1.214  -1.291  -1.194
174   K         0.447   0.323   0.159   0.526   0.398   0.144   0.448   0.259  -0.046
175   L         1.159   1.182   1.239   0.876   0.908   1.031   1.224   1.256   1.343
176   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
177   N        -2.114  -2.127  -1.867  -1.675  -1.718  -1.336  -1.657  -1.632  -1.342
178   A        -0.231  -0.283  -0.534  -0.191  -0.259  -0.661  -0.216  -0.295  -0.629
179   P         1.159   1.182   1.239   1.291   1.320   1.399   1.224   1.256   1.343
180   V        -1.132  -1.157  -1.113  -1.093  -1.118  -1.042  -1.103  -1.108  -1.024
181   F        -0.682  -0.753  -0.622  -0.690  -0.754  -0.536  -0.881  -0.941  -0.753
182   T         1.159   1.182   1.239   1.372   1.391   1.471   1.224   1.256   1.343
183   L         1.159   1.182   1.239   0.876   0.908   1.031   1.224   1.256   1.343
184   L         0.447   0.571   0.185   0.224   0.382  -0.137   0.448   0.622  -0.000
185   N        -1.460  -1.486  -1.497  -1.064  -1.134  -1.192  -1.214  -1.242  -1.189
186   A        -0.869  -0.793  -1.062  -0.760  -0.675  -1.026  -0.771  -0.692  -0.967
187   Q         0.447   0.313   0.177   0.705   0.523   0.277   0.448   0.244  -0.015
188   K        -1.132  -1.067  -1.099  -1.213  -1.046  -1.171  -1.103  -1.006  -1.007
189   E         1.159   1.182   1.239   1.131   1.186   1.257   1.224   1.256   1.343
190   A        -0.044   0.050   0.077  -0.346  -0.161  -0.160  -0.352  -0.195  -0.193
191   L        -0.480  -0.571  -0.613  -0.758  -0.910  -0.926  -0.549  -0.691  -0.735
192   K        -1.789  -1.729  -1.544  -1.572  -1.514  -1.251  -1.534  -1.466  -1.229
193   K        -1.188  -1.203  -1.144  -1.233  -1.292  -1.187  -1.140  -1.161  -1.053
194   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
195   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
196   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
197   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P49435
Output conservation file - output/P49435
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
