1     M         0.987   0.999   1.039   1.202   1.221   1.265   1.040   1.057   1.113
2     A        -1.438  -1.501  -1.468  -1.217  -1.339  -1.326  -1.300  -1.362  -1.281
3     E        -1.613  -1.619  -1.495  -1.625  -1.626  -1.421  -1.488  -1.483  -1.307
4     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
5     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
6     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
7     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
8     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
9     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
10    N        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
11    E        -2.060  -2.056  -1.905  -1.962  -1.954  -1.707  -1.690  -1.670  -1.470
12    R        -0.256  -0.249  -0.220  -0.434  -0.420  -0.369  -0.520  -0.512  -0.484
13    M        -1.609  -1.499  -1.480  -1.668  -1.535  -1.515  -1.495  -1.371  -1.293
14    Y         0.987   0.999   1.039   1.148   1.162   1.216   1.040   1.057   1.113
15    I        -0.707  -0.871  -0.922  -0.713  -0.931  -0.985  -0.715  -0.924  -0.978
16    S         0.107  -0.001  -0.141   0.315   0.165  -0.086   0.065  -0.101  -0.346
17    Y         0.987   0.999   1.039   1.148   1.162   1.216   1.040   1.057   1.113
18    N         0.987   0.999   1.039   1.102   1.121   1.174   1.040   1.057   1.113
19    N        -0.256  -0.314  -0.227  -0.298  -0.428  -0.308  -0.520  -0.625  -0.496
20    I        -0.256  -0.283  -0.221  -0.529  -0.568  -0.463  -0.520  -0.570  -0.486
21    H         0.987   0.999   1.039   1.173   1.194   1.238   1.040   1.057   1.113
22    K         0.107  -0.001  -0.141   0.106  -0.031  -0.237   0.065  -0.101  -0.346
23    L         0.107   0.022  -0.139   0.062  -0.043  -0.243   0.065  -0.066  -0.343
24    C         0.987   0.999   1.039   1.260   1.274   1.317   1.040   1.057   1.113
25    Q         0.107   0.022  -0.139   0.259   0.140  -0.142   0.065  -0.066  -0.343
26    G        -2.060  -1.932  -1.891  -2.070  -1.871  -1.813  -1.690  -1.564  -1.459
27    V        -1.438  -1.405  -1.454  -1.335  -1.284  -1.401  -1.300  -1.258  -1.267
28    A         0.987   0.999   1.039   0.970   0.985   1.053   1.040   1.057   1.113
29    K        -0.987  -1.053  -0.955  -1.178  -1.266  -1.084  -1.105  -1.170  -1.021
30    H        -1.438  -1.204  -1.448  -1.321  -0.998  -1.307  -1.300  -1.031  -1.261
31    I         0.987   0.999   1.039   0.821   0.856   0.918   1.040   1.057   1.113
32    L         0.010   0.134  -0.143  -0.071   0.092  -0.257  -0.083   0.105  -0.351
33    A        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
34    R        -1.158  -1.095  -0.969  -1.400  -1.315  -1.109  -1.300  -1.213  -1.036
35    N        -1.609  -1.471  -1.476  -1.635  -1.433  -1.411  -1.495  -1.350  -1.290
36    E        -0.366  -0.315  -0.221  -0.543  -0.420  -0.286  -0.715  -0.627  -0.486
37    R        -0.707  -0.783  -0.908  -0.501  -0.613  -0.848  -0.715  -0.813  -0.961
38    P         0.987   0.999   1.039   1.111   1.121   1.181   1.040   1.057   1.113
39    D         0.107  -0.001  -0.141   0.160   0.016  -0.202   0.065  -0.101  -0.346
40    I        -0.987  -0.932  -0.941  -1.184  -1.129  -1.138  -1.105  -1.045  -1.005
41    I        -0.256  -0.283  -0.221  -0.436  -0.456  -0.344  -0.520  -0.570  -0.486
42    I         0.987   0.999   1.039   0.821   0.856   0.918   1.040   1.057   1.113
43    A         0.987   0.999   1.039   0.970   0.985   1.053   1.040   1.057   1.113
44    I         0.987   0.999   1.039   0.821   0.856   0.918   1.040   1.057   1.113
45    T        -0.366  -0.315  -0.221  -0.577  -0.499  -0.340  -0.715  -0.627  -0.486
46    G         0.987   0.999   1.039   0.979   0.991   1.061   1.040   1.057   1.113
47    G         0.987   0.999   1.039   0.979   0.991   1.061   1.040   1.057   1.113
48    G         0.987   0.999   1.039   0.979   0.991   1.061   1.040   1.057   1.113
49    M        -0.987  -1.053  -0.955  -0.740  -0.837  -0.703  -1.105  -1.170  -1.021
50    I         0.107  -0.001  -0.141   0.021  -0.115  -0.334   0.065  -0.101  -0.346
51    P         0.987   0.999   1.039   1.111   1.121   1.181   1.040   1.057   1.113
52    A         0.987   0.999   1.039   0.970   0.985   1.053   1.040   1.057   1.113
53    R         0.987   0.999   1.039   1.081   1.099   1.154   1.040   1.057   1.113
54    I         0.987   0.999   1.039   0.821   0.856   0.918   1.040   1.057   1.113
55    I        -0.366  -0.315  -0.221  -0.844  -0.739  -0.579  -0.715  -0.627  -0.486
56    R         0.987   0.999   1.039   1.081   1.099   1.154   1.040   1.057   1.113
57    S        -0.256  -0.274  -0.219  -0.254  -0.295  -0.237  -0.520  -0.555  -0.482
58    F         0.107   0.132  -0.127   0.402   0.434   0.048   0.065   0.102  -0.324
59    L         0.107   0.132  -0.127  -0.017   0.022  -0.394   0.065   0.102  -0.324
60    K         0.107   0.132  -0.127   0.102   0.144  -0.225   0.065   0.102  -0.324
61    T        -2.060  -2.029  -1.894  -1.867  -1.848  -1.636  -1.690  -1.649  -1.462
62    K        -0.987  -0.918  -0.940  -1.052  -0.963  -0.943  -1.105  -1.031  -1.003
63    G         0.987   0.999   1.039   0.979   0.991   1.061   1.040   1.057   1.113
64    Q        -0.366  -0.315  -0.221  -0.445  -0.348  -0.215  -0.715  -0.627  -0.486
65    K         0.107  -0.001  -0.141   0.108  -0.031  -0.233   0.065  -0.101  -0.346
66    N         0.987   0.999   1.039   1.102   1.121   1.174   1.040   1.057   1.113
67    I         0.987   0.999   1.039   0.821   0.856   0.918   1.040   1.057   1.113
68    P         0.987   0.999   1.039   1.111   1.121   1.181   1.040   1.057   1.113
69    I         0.987   0.999   1.039   0.821   0.856   0.918   1.040   1.057   1.113
70    Q         0.987   0.999   1.039   1.069   1.090   1.143   1.040   1.057   1.113
71    A         0.987   0.999   1.039   0.970   0.985   1.053   1.040   1.057   1.113
72    I         0.987   0.999   1.039   0.821   0.856   0.918   1.040   1.057   1.113
73    G         0.987   0.999   1.039   0.979   0.991   1.061   1.040   1.057   1.113
74    L         0.987   0.999   1.039   0.821   0.841   0.918   1.040   1.057   1.113
75    S         0.987   0.999   1.039   1.115   1.136   1.185   1.040   1.057   1.113
76    L         0.987   0.999   1.039   0.821   0.841   0.918   1.040   1.057   1.113
77    Y         0.987   0.999   1.039   1.148   1.162   1.216   1.040   1.057   1.113
78    E         0.987   0.999   1.039   0.870   0.890   0.962   1.040   1.057   1.113
79    D        -0.987  -1.053  -0.955  -1.124  -1.217  -1.045  -1.105  -1.170  -1.021
80    L         0.987   0.999   1.039   0.821   0.841   0.918   1.040   1.057   1.113
81    G         0.107   0.022  -0.139   0.178   0.051  -0.200   0.065  -0.066  -0.343
82    L        -1.438  -1.544  -1.483  -1.465  -1.626  -1.502  -1.300  -1.403  -1.295
83    D        -0.256  -0.187  -0.206  -0.426  -0.310  -0.360  -0.520  -0.405  -0.460
84    N        -2.060  -2.056  -1.905  -1.811  -1.809  -1.620  -1.690  -1.670  -1.470
85    S        -2.511  -2.433  -2.244  -2.233  -2.137  -1.864  -1.885  -1.807  -1.581
86    V        -0.987  -0.828  -0.932  -1.154  -0.918  -1.047  -1.105  -0.895  -0.993
87    E         0.107   0.224  -0.121   0.057   0.214  -0.287   0.065   0.235  -0.313
88    T        -1.072  -1.053  -0.955  -1.115  -1.081  -0.925  -1.207  -1.167  -1.020
89    I        -0.256  -0.314  -0.227  -0.480  -0.558  -0.404  -0.520  -0.625  -0.496
90    G         0.987   0.999   1.039   0.979   0.991   1.061   1.040   1.057   1.113
91    K        -1.438  -1.544  -1.483  -1.452  -1.630  -1.534  -1.300  -1.403  -1.295
92    E         0.107  -0.001  -0.141   0.045  -0.111  -0.348   0.065  -0.101  -0.346
93    V         0.987   0.999   1.039   1.009   1.022   1.089   1.040   1.057   1.113
94    I        -1.438  -1.544  -1.483  -1.495  -1.650  -1.546  -1.300  -1.403  -1.295
95    R         0.987   0.999   1.039   1.081   1.099   1.154   1.040   1.057   1.113
96    T         0.987   0.999   1.039   1.022   1.039   1.100   1.040   1.057   1.113
97    Q         0.987   0.999   1.039   1.069   1.090   1.143   1.040   1.057   1.113
98    W         0.987   0.999   1.039   1.235   1.251   1.295   1.040   1.057   1.113
99    L         0.987   0.999   1.039   0.821   0.841   0.918   1.040   1.057   1.113
100   D         0.987   0.999   1.039   0.949   0.970   1.034   1.040   1.057   1.113
101   F         0.107  -0.001  -0.141   0.335   0.166  -0.121   0.065  -0.101  -0.346
102   G        -0.987  -1.053  -0.955  -0.987  -1.078  -0.919  -1.105  -1.170  -1.021
103   A        -0.256  -0.274  -0.219  -0.386  -0.429  -0.343  -0.520  -0.555  -0.482
104   L         0.987   0.999   1.039   0.821   0.841   0.918   1.040   1.057   1.113
105   N        -1.609  -1.506  -1.480  -1.648  -1.498  -1.442  -1.495  -1.376  -1.293
106   Q        -1.072  -1.107  -0.959  -1.164  -1.191  -0.972  -1.207  -1.228  -1.025
107   H         0.987   0.999   1.039   1.159   1.174   1.225   1.040   1.057   1.113
108   F        -0.987  -0.986  -0.953  -0.757  -0.762  -0.712  -1.105  -1.103  -1.019
109   D        -1.438  -1.544  -1.483  -1.387  -1.571  -1.485  -1.300  -1.403  -1.295
110   S        -0.987  -1.053  -0.955  -0.944  -1.048  -0.903  -1.105  -1.170  -1.021
111   L         0.987   0.999   1.039   0.821   0.841   0.918   1.040   1.057   1.113
112   I         0.107   0.022  -0.139   0.021  -0.082  -0.331   0.065  -0.066  -0.343
113   G         0.987   0.999   1.039   0.979   0.991   1.061   1.040   1.057   1.113
114   K         0.987   0.999   1.039   0.888   0.914   0.978   1.040   1.057   1.113
115   K        -1.158  -1.112  -0.969  -1.256  -1.163  -0.954  -1.300  -1.235  -1.036
116   V        -0.256  -0.149  -0.183  -0.529  -0.344  -0.404  -0.520  -0.342  -0.419
117   L         0.987   0.999   1.039   0.821   0.841   0.918   1.040   1.057   1.113
118   I         0.987   0.999   1.039   0.821   0.856   0.918   1.040   1.057   1.113
119   V         0.987   0.999   1.039   1.009   1.022   1.089   1.040   1.057   1.113
120   D         0.987   0.999   1.039   0.949   0.970   1.034   1.040   1.057   1.113
121   E         0.987   0.999   1.039   0.870   0.890   0.962   1.040   1.057   1.113
122   V         0.987   0.999   1.039   1.009   1.022   1.089   1.040   1.057   1.113
123   D         0.987   0.999   1.039   0.949   0.970   1.034   1.040   1.057   1.113
124   D         0.987   0.999   1.039   0.949   0.970   1.034   1.040   1.057   1.113
125   T         0.987   0.999   1.039   1.022   1.039   1.100   1.040   1.057   1.113
126   R         0.987   0.999   1.039   1.081   1.099   1.154   1.040   1.057   1.113
127   T         0.987   0.999   1.039   1.022   1.039   1.100   1.040   1.057   1.113
128   T         0.987   0.999   1.039   1.022   1.039   1.100   1.040   1.057   1.113
129   L         0.987   0.999   1.039   0.821   0.841   0.918   1.040   1.057   1.113
130   H         0.107  -0.001  -0.141   0.344   0.183  -0.101   0.065  -0.101  -0.346
131   Y         0.987   0.999   1.039   1.148   1.162   1.216   1.040   1.057   1.113
132   A         0.987   0.999   1.039   0.970   0.985   1.053   1.040   1.057   1.113
133   V        -0.256  -0.242  -0.217  -0.442  -0.430  -0.425  -0.520  -0.498  -0.478
134   S        -1.609  -1.652  -1.484  -1.505  -1.570  -1.330  -1.495  -1.512  -1.297
135   E         0.987   0.999   1.039   0.870   0.890   0.962   1.040   1.057   1.113
136   L         0.987   0.999   1.039   0.821   0.841   0.918   1.040   1.057   1.113
137   E        -0.366  -0.330  -0.227  -0.621  -0.541  -0.379  -0.715  -0.653  -0.496
138   K         0.107   0.022  -0.139   0.102  -0.005  -0.243   0.065  -0.066  -0.343
139   E        -0.366  -0.315  -0.221  -0.711  -0.607  -0.453  -0.715  -0.627  -0.486
140   I        -0.256  -0.196  -0.208  -0.442  -0.334  -0.405  -0.520  -0.420  -0.463
141   A        -0.987  -0.901  -0.933  -1.088  -0.989  -0.975  -1.105  -1.005  -0.996
142   E        -0.256  -0.283  -0.221  -0.502  -0.558  -0.451  -0.520  -0.570  -0.486
143   Q         0.107  -0.001  -0.141   0.259   0.106  -0.146   0.065  -0.101  -0.346
144   Q        -0.987  -1.053  -0.955  -0.976  -1.080  -0.927  -1.105  -1.170  -1.021
145   K        -0.707  -0.705  -0.896  -0.668  -0.667  -0.937  -0.715  -0.719  -0.946
146   V        -1.438  -1.370  -1.451  -1.386  -1.303  -1.396  -1.300  -1.223  -1.264
147   L         0.987   0.999   1.039   0.821   0.841   0.918   1.040   1.057   1.113
148   N        -0.256  -0.283  -0.221  -0.285  -0.351  -0.276  -0.520  -0.570  -0.486
149   R         0.107  -0.001  -0.141   0.273   0.116  -0.132   0.065  -0.101  -0.346
150   M        -1.072  -0.975  -0.958  -1.311  -1.162  -1.073  -1.207  -1.079  -1.024
151   S        -1.609  -1.499  -1.480  -1.507  -1.372  -1.321  -1.495  -1.371  -1.293
152   E         0.987   0.999   1.039   0.870   0.890   0.962   1.040   1.057   1.113
153   E        -0.987  -0.914  -0.939  -1.231  -1.137  -1.136  -1.105  -1.024  -1.002
154   T         0.987   0.999   1.039   1.022   1.039   1.100   1.040   1.057   1.113
155   V        -1.438  -1.370  -1.451  -1.311  -1.207  -1.370  -1.300  -1.223  -1.264
156   I        -0.707  -0.608  -0.884  -0.493  -0.347  -0.873  -0.715  -0.584  -0.931
157   S        -1.438  -1.544  -1.483  -1.158  -1.345  -1.269  -1.300  -1.403  -1.295
158   I         0.107   0.132  -0.127   0.021   0.070  -0.312   0.065   0.102  -0.324
159   F         0.987   0.999   1.039   1.173   1.189   1.238   1.040   1.057   1.113
160   V         0.987   0.999   1.039   1.009   1.022   1.089   1.040   1.057   1.113
161   L         0.107   0.022  -0.139   0.021  -0.097  -0.331   0.065  -0.066  -0.343
162   H         0.987   0.999   1.039   1.173   1.194   1.238   1.040   1.057   1.113
163   N         0.987   0.999   1.039   1.102   1.121   1.174   1.040   1.057   1.113
164   K         0.987   0.999   1.039   0.888   0.914   0.978   1.040   1.057   1.113
165   D         0.107   0.022  -0.139   0.148   0.031  -0.225   0.065  -0.066  -0.343
166   K         0.987   0.999   1.039   0.888   0.914   0.978   1.040   1.057   1.113
167   P         0.107   0.224  -0.121   0.285   0.436  -0.108   0.065   0.235  -0.313
168   K         0.987   0.999   1.039   0.888   0.914   0.978   1.040   1.057   1.113
169   R        -0.987  -0.978  -0.931  -1.059  -1.024  -0.938  -1.105  -1.068  -0.990
170   A        -0.707  -0.705  -0.896  -0.610  -0.630  -0.913  -0.715  -0.719  -0.946
171   G        -1.609  -1.584  -1.476  -1.630  -1.586  -1.417  -1.495  -1.456  -1.289
172   L         0.987   0.999   1.039   0.821   0.841   0.918   1.040   1.057   1.113
173   P         0.107   0.022  -0.139   0.297   0.166  -0.116   0.065  -0.066  -0.343
174   D        -1.438  -1.370  -1.451  -1.473  -1.386  -1.515  -1.300  -1.223  -1.264
175   S        -0.987  -0.932  -0.941  -1.146  -1.085  -1.031  -1.105  -1.045  -1.005
176   M        -0.987  -0.929  -0.946  -1.014  -0.980  -1.019  -1.105  -1.049  -1.010
177   M        -1.438  -1.487  -1.467  -1.167  -1.304  -1.310  -1.300  -1.348  -1.280
178   N        -0.707  -0.724  -0.899  -0.574  -0.600  -0.835  -0.715  -0.741  -0.949
179   S        -1.438  -1.487  -1.467  -1.177  -1.276  -1.268  -1.300  -1.348  -1.280
180   G         0.010   0.126  -0.152   0.009   0.167  -0.287  -0.083   0.093  -0.366
181   R         0.107   0.022  -0.139   0.307   0.193  -0.056   0.065  -0.066  -0.343
182   Y         0.987   0.999   1.039   1.148   1.162   1.216   1.040   1.057   1.113
183   I        -0.987  -0.930  -0.946  -1.140  -1.024  -1.053  -1.105  -1.050  -1.011
184   A         0.107   0.022  -0.139   0.172   0.050  -0.201   0.065  -0.066  -0.343
185   A         0.107   0.022  -0.139   0.163   0.038  -0.220   0.065  -0.066  -0.343
186   Q        -2.060  -1.979  -1.887  -1.887  -1.768  -1.606  -1.690  -1.604  -1.456
187   T         0.107   0.022  -0.139   0.239   0.123  -0.124   0.065  -0.066  -0.343
188   V        -0.256  -0.274  -0.219  -0.351  -0.396  -0.314  -0.520  -0.555  -0.482
189   P         0.107  -0.001  -0.141   0.303   0.137  -0.107   0.065  -0.101  -0.346
190   D         0.987   0.999   1.039   0.949   0.970   1.034   1.040   1.057   1.113
191   K        -0.987  -1.043  -0.954  -1.133  -1.197  -1.013  -1.105  -1.160  -1.020
192   W         0.987   0.999   1.039   1.235   1.251   1.295   1.040   1.057   1.113
193   L        -0.366  -0.315  -0.221  -0.844  -0.735  -0.579  -0.715  -0.627  -0.486
194   C        -0.707  -0.765  -0.906  -0.288  -0.374  -0.640  -0.715  -0.788  -0.959
195   Y         0.987   0.999   1.039   1.148   1.162   1.216   1.040   1.057   1.113
196   P         0.987   0.999   1.039   1.111   1.121   1.181   1.040   1.057   1.113
197   W         0.987   0.999   1.039   1.235   1.251   1.295   1.040   1.057   1.113
198   D         0.107   0.204  -0.124   0.057   0.188  -0.292   0.065   0.206  -0.319
199   A         0.987   0.999   1.039   0.970   0.985   1.053   1.040   1.057   1.113
200   E        -2.060  -1.987  -1.889  -1.959  -1.840  -1.741  -1.690  -1.613  -1.457
201   D         0.107  -0.001  -0.141   0.167   0.025  -0.187   0.065  -0.101  -0.346
202   I         0.987   0.999   1.039   0.821   0.856   0.918   1.040   1.057   1.113
203   E         0.987   0.999   1.039   0.870   0.890   0.962   1.040   1.057   1.113
204   E         0.987   0.999   1.039   0.870   0.890   0.962   1.040   1.057   1.113
205   H         0.987   0.999   1.039   1.173   1.194   1.238   1.040   1.057   1.113
206   T        -0.256  -0.314  -0.227  -0.372  -0.497  -0.365  -0.520  -0.625  -0.496
207   M        -2.060  -2.030  -1.894  -1.914  -1.845  -1.654  -1.690  -1.650  -1.461
208   L        -1.609  -1.653  -1.485  -1.494  -1.520  -1.267  -1.495  -1.513  -1.297
209   A         0.107   0.022  -0.139   0.169   0.045  -0.207   0.065  -0.066  -0.343
210   K        -0.987  -0.929  -0.946  -1.204  -1.125  -1.139  -1.105  -1.049  -1.010
211   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
212   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
213   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
214   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
215   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
216   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
217   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
218   A        -1.609  -1.600  -1.478  -1.630  -1.629  -1.455  -1.495  -1.465  -1.291
219   Q        -0.707  -0.871  -0.922  -0.555  -0.820  -0.958  -0.715  -0.924  -0.978
220   G        -1.438  -1.544  -1.483  -1.352  -1.552  -1.455  -1.300  -1.403  -1.295
221   H        -1.438  -1.501  -1.468  -1.167  -1.288  -1.248  -1.300  -1.362  -1.281
222   D        -0.987  -1.034  -0.946  -0.994  -1.049  -0.897  -1.105  -1.147  -1.010
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P47165
Output conservation file - output/P47165
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
