1     M         2.283   2.344   2.477   2.515   2.591   2.821   2.648   2.754   3.013
2     A         0.006   0.013   0.069   0.178   0.193   0.255  -0.258  -0.259  -0.188
3     Q        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
4     A        -0.628  -0.519  -0.542  -1.199  -1.103  -1.094  -0.710  -0.588  -0.586
5     L         0.383   0.272   0.216  -0.041  -0.254  -0.340   0.323   0.146   0.044
6     N         1.296   1.444   1.261   1.405   1.610   1.363   1.356   1.608   1.254
7     S         0.068   0.031   0.125  -0.120  -0.195  -0.055  -0.194  -0.232  -0.111
8     T        -1.134  -1.087  -1.072  -0.660  -0.581  -0.554  -0.969  -0.916  -0.872
9     N        -0.438  -0.523  -0.540  -0.284  -0.405  -0.449  -0.452  -0.559  -0.573
10    I        -0.628  -0.606  -0.547  -1.123  -1.099  -1.000  -0.710  -0.677  -0.591
11    A        -1.134  -1.123  -1.065  -1.231  -1.238  -1.151  -0.969  -0.951  -0.864
12    F        -0.628  -0.569  -0.541  -0.961  -0.913  -0.863  -0.710  -0.641  -0.585
13    F         0.068   0.150   0.131  -0.369  -0.263  -0.326  -0.194  -0.089  -0.107
14    R         1.296   1.193   1.190   1.618   1.484   1.483   1.356   1.158   1.112
15    V        -0.628  -0.654  -0.551  -0.557  -0.555  -0.422  -0.710  -0.729  -0.595
16    A        -1.134  -1.171  -1.106  -1.073  -1.092  -1.032  -0.969  -0.997  -0.906
17    F         0.068   0.022   0.117  -0.412  -0.475  -0.349  -0.194  -0.248  -0.127
18    L         1.296   1.319   1.221   0.945   0.956   0.859   1.356   1.390   1.174
19    F        -1.134  -1.140  -1.087  -1.318  -1.289  -1.201  -0.969  -0.968  -0.887
20    T        -1.134  -1.171  -1.106  -0.895  -1.009  -0.880  -0.969  -0.997  -0.906
21    I         0.068   0.050   0.142  -0.492  -0.545  -0.397  -0.194  -0.201  -0.082
22    A         1.296   1.193   1.190   1.362   1.223   1.235   1.356   1.158   1.112
23    F         0.068   0.003   0.100  -0.111  -0.223  -0.049  -0.194  -0.280  -0.154
24    F         0.383   0.307   0.248   0.246   0.117  -0.008   0.323   0.199   0.095
25    C        -0.438  -0.512  -0.510  -0.200  -0.385  -0.432  -0.452  -0.547  -0.536
26    L        -0.628  -0.606  -0.547  -0.911  -0.912  -0.788  -0.710  -0.677  -0.591
27    K         0.068   0.003   0.100   0.325   0.252   0.399  -0.194  -0.280  -0.154
28    N         0.765   0.806   0.924   0.626   0.709   0.863   0.323   0.378   0.551
29    V         0.765   0.818   0.945   0.631   0.731   0.932   0.323   0.405   0.597
30    N        -0.438  -0.523  -0.540  -0.202  -0.290  -0.330  -0.452  -0.559  -0.573
31    S        -0.628  -0.649  -0.573  -0.749  -0.807  -0.692  -0.710  -0.723  -0.619
32    I         0.383   0.293   0.229   0.335   0.172   0.008   0.323   0.178   0.065
33    L         0.383   0.272   0.216  -0.000  -0.192  -0.271   0.323   0.146   0.044
34    Q        -0.628  -0.594  -0.558  -0.365  -0.279  -0.244  -0.710  -0.660  -0.600
35    N         0.190   0.139   0.145   0.216   0.153   0.146  -0.009  -0.092  -0.090
36    T        -0.513  -0.408  -0.507  -0.209  -0.054  -0.165  -0.579  -0.441  -0.543
37    Y        -0.080  -0.062   0.020  -0.567  -0.605  -0.468  -0.389  -0.368  -0.263
38    F        -0.513  -0.434  -0.452  -0.646  -0.496  -0.534  -0.579  -0.462  -0.469
39    I        -1.111  -1.116  -1.081  -1.454  -1.427  -1.409  -0.958  -0.954  -0.884
40    V         0.084   0.103   0.118  -0.458  -0.475  -0.430  -0.199  -0.160  -0.129
41    L         0.539   0.397   0.285   0.190  -0.032  -0.196   0.560   0.341   0.155
42    T         0.190   0.361   0.199   0.423   0.667   0.445  -0.009   0.250   0.004
43    Q         1.385   1.273   1.242   1.711   1.567   1.516   1.509   1.306   1.216
44    A         1.385   1.273   1.242   1.450   1.304   1.261   1.509   1.306   1.216
45    M         2.283   2.344   2.477   2.515   2.591   2.821   2.648   2.754   3.013
46    N        -0.513  -0.412  -0.506  -0.222  -0.058  -0.208  -0.579  -0.469  -0.547
47    L         0.973   0.915   0.984   0.380   0.257   0.355   0.750   0.607   0.682
48    P         0.539   0.478   0.319   0.958   0.887   0.662   0.560   0.458   0.209
49    Q        -1.111  -1.034  -1.050  -1.014  -0.902  -0.923  -0.958  -0.874  -0.855
50    L         0.539   0.572   0.368   0.190   0.217  -0.068   0.560   0.610   0.287
51    T        -1.978  -1.946  -1.884  -1.574  -1.521  -1.423  -1.338  -1.302  -1.209
52    L        -0.513  -0.434  -0.487  -0.894  -0.818  -0.822  -0.579  -0.494  -0.524
53    S        -1.111  -1.053  -1.063  -0.757  -0.648  -0.640  -0.958  -0.888  -0.866
54    R        -0.947  -0.888  -0.964  -0.413  -0.311  -0.425  -0.768  -0.696  -0.757
55    Y        -1.544  -1.581  -1.517  -1.023  -1.062  -0.964  -1.148  -1.168  -1.075
56    S         0.084   0.103   0.118  -0.013   0.061   0.061  -0.199  -0.160  -0.129
57    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
58    G        -0.513  -0.506  -0.518  -0.219  -0.205  -0.225  -0.579  -0.551  -0.552
59    Q         0.084   0.030   0.112  -0.184  -0.284  -0.142  -0.199  -0.251  -0.139
60    L         2.283   2.344   2.477   1.790   1.855   2.069   2.648   2.754   3.013
61    G         1.385   1.273   1.242   1.690   1.528   1.495   1.509   1.306   1.216
62    L         0.190   0.274   0.211  -0.196  -0.109  -0.151  -0.009   0.110   0.018
63    F        -0.947  -1.044  -1.049  -0.866  -1.028  -1.010  -0.768  -0.868  -0.849
64    A         0.084   0.023   0.136  -0.013  -0.072   0.097  -0.199  -0.263  -0.110
65    L        -0.947  -0.902  -0.970  -1.124  -1.093  -1.123  -0.768  -0.711  -0.764
66    L         0.190   0.188   0.145  -0.342  -0.371  -0.408  -0.009  -0.045  -0.088
67    F         0.973   1.014   1.003   0.536   0.571   0.458   0.750   0.809   0.723
68    T        -1.544  -1.536  -1.478  -1.422  -1.509  -1.354  -1.148  -1.131  -1.042
69    L        -1.544  -1.564  -1.504  -1.671  -1.681  -1.553  -1.148  -1.155  -1.064
70    N        -0.947  -0.798  -0.992  -1.258  -1.054  -1.318  -0.768  -0.594  -0.788
71    G         0.788   0.814   0.924   0.629   0.659   0.844   0.370   0.394   0.550
72    V        -0.513  -0.545  -0.505  -0.757  -0.793  -0.755  -0.579  -0.609  -0.543
73    H        -0.513  -0.490  -0.518  -0.287  -0.266  -0.281  -0.579  -0.538  -0.555
74    D         1.385   1.273   1.242   1.726   1.590   1.559   1.509   1.306   1.216
75    L        -0.243  -0.331  -0.442  -0.554  -0.684  -0.846  -0.199  -0.315  -0.449
76    I        -0.243  -0.148  -0.363  -0.223  -0.086  -0.470  -0.199  -0.068  -0.344
77    P         1.385   1.273   1.242   1.699   1.551   1.513   1.509   1.306   1.216
78    L         2.283   2.344   2.477   1.790   1.855   2.069   2.648   2.754   3.013
79    L         0.539   0.618   0.366   0.263   0.350   0.048   0.560   0.679   0.284
80    E        -0.513  -0.604  -0.554  -0.360  -0.432  -0.367  -0.579  -0.673  -0.597
81    N        -1.111  -1.177  -1.069  -0.792  -0.844  -0.706  -0.958  -1.004  -0.873
82    N         1.385   1.390   1.257   1.527   1.569   1.382   1.509   1.514   1.246
83    V        -1.544  -1.556  -1.500  -1.319  -1.389  -1.297  -1.148  -1.149  -1.061
84    K        -1.111  -1.128  -1.066  -0.950  -1.027  -0.909  -0.958  -0.964  -0.870
85    Y         0.539   0.470   0.323   0.897   0.777   0.607   0.560   0.448   0.215
86    F         2.283   2.344   2.477   2.131   2.202   2.422   2.648   2.754   3.013
87    Q        -1.111  -1.124  -1.084  -0.725  -0.727  -0.652  -0.958  -0.960  -0.886
88    S        -0.947  -0.998  -1.006  -0.630  -0.691  -0.702  -0.768  -0.820  -0.803
89    V         0.190   0.243   0.229  -0.008   0.070   0.007  -0.009   0.089   0.051
90    V         0.539   0.397   0.285   0.746   0.565   0.431   0.560   0.341   0.155
91    P         2.283   2.344   2.477   2.494   2.578   2.799   2.648   2.754   3.013
92    V        -1.111  -1.053  -1.063  -1.261  -1.196  -1.221  -0.958  -0.888  -0.866
93    R         2.283   2.344   2.477   2.504   2.596   2.810   2.648   2.754   3.013
94    L         0.539   0.397   0.285   0.189  -0.029  -0.198   0.560   0.341   0.155
95    L        -1.544  -1.424  -1.468  -1.474  -1.278  -1.369  -1.148  -1.027  -1.033
96    I        -1.544  -1.564  -1.504  -1.509  -1.558  -1.473  -1.148  -1.155  -1.064
97    F        -0.513  -0.604  -0.554  -0.617  -0.743  -0.695  -0.579  -0.673  -0.597
98    F         2.283   2.344   2.477   2.131   2.202   2.422   2.648   2.754   3.013
99    I        -0.947  -0.933  -0.986  -0.640  -0.622  -0.669  -0.768  -0.744  -0.781
100   L         0.973   1.038   1.015   0.382   0.453   0.407   0.750   0.856   0.748
101   T        -0.513  -0.462  -0.490  -0.335  -0.283  -0.272  -0.579  -0.516  -0.518
102   S         0.539   0.533   0.349   0.712   0.705   0.467   0.560   0.536   0.257
103   I        -0.513  -0.506  -0.518  -0.568  -0.556  -0.646  -0.579  -0.551  -0.552
104   S        -0.513  -0.494  -0.504  -0.416  -0.410  -0.381  -0.579  -0.566  -0.543
105   Y         0.539   0.574   0.345   0.839   0.888   0.546   0.560   0.614   0.250
106   L        -0.513  -0.490  -0.499  -0.998  -0.983  -0.957  -0.579  -0.546  -0.537
107   W        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
108   E        -1.544  -1.559  -1.491  -1.062  -1.051  -0.972  -1.148  -1.151  -1.054
109   S        -1.111  -1.127  -1.047  -0.854  -0.814  -0.730  -0.958  -0.963  -0.853
110   N        -0.513  -0.608  -0.544  -0.417  -0.508  -0.439  -0.579  -0.676  -0.588
111   F        -1.544  -1.556  -1.500  -1.586  -1.526  -1.457  -1.148  -1.149  -1.061
112   Y         0.190   0.188   0.145   0.243   0.181   0.120  -0.009  -0.045  -0.088
113   V        -0.513  -0.524  -0.492  -0.779  -0.779  -0.798  -0.579  -0.577  -0.525
114   H         0.788   0.881   0.967   0.773   0.900   1.038   0.370   0.537   0.644
115   N         1.385   1.483   1.278   1.505   1.665   1.373   1.509   1.674   1.286
116   N         0.788   0.806   0.923   0.555   0.584   0.741   0.370   0.378   0.550
117   S        -1.111  -1.029  -1.055  -1.341  -1.285  -1.253  -0.958  -0.869  -0.860
118   V         0.190   0.137   0.164   0.140   0.085   0.102  -0.009  -0.069  -0.056
119   F         1.385   1.379   1.287   1.328   1.311   1.200   1.509   1.495   1.305
120   I        -0.677  -0.648  -0.579  -0.693  -0.623  -0.530  -0.768  -0.723  -0.629
121   Y         0.539   0.397   0.285   0.819   0.592   0.406   0.560   0.341   0.155
122   C        -1.111  -1.102  -1.068  -0.768  -0.796  -0.715  -0.958  -0.938  -0.871
123   F         1.385   1.379   1.287   1.252   1.225   1.066   1.509   1.495   1.305
124   A        -1.111  -1.024  -1.052  -1.087  -0.958  -0.992  -0.958  -0.870  -0.856
125   E         0.973   0.919   0.966   1.001   0.946   0.972   0.750   0.616   0.643
126   V        -0.080  -0.050   0.031  -0.578  -0.611  -0.494  -0.389  -0.353  -0.248
127   W         0.539   0.397   0.285   1.003   0.793   0.579   0.560   0.341   0.155
128   I        -1.111  -1.144  -1.073  -0.893  -0.910  -0.768  -0.958  -0.976  -0.876
129   N         0.539   0.397   0.285   0.771   0.616   0.431   0.560   0.341   0.155
130   F         2.283   2.344   2.477   2.131   2.202   2.422   2.648   2.754   3.013
131   L         0.539   0.397   0.285   0.253   0.064  -0.079   0.560   0.341   0.155
132   L        -0.513  -0.558  -0.513  -0.689  -0.693  -0.628  -0.579  -0.609  -0.546
133   Y         0.190   0.245   0.169   0.117   0.159  -0.014  -0.009   0.041  -0.054
134   N        -0.243  -0.338  -0.437  -0.172  -0.315  -0.565  -0.199  -0.323  -0.441
135   A         0.973   1.012   1.071   0.840   0.905   0.969   0.750   0.805   0.868
136   I        -0.243  -0.182  -0.378  -0.502  -0.423  -0.678  -0.199  -0.127  -0.364
137   R         1.385   1.273   1.242   1.710   1.568   1.524   1.509   1.306   1.216
138   E         0.788   0.834   0.945   0.703   0.805   0.938   0.370   0.437   0.597
139   E         2.283   2.344   2.477   2.332   2.459   2.631   2.648   2.754   3.013
140   K         0.973   1.042   1.041   1.164   1.274   1.257   0.750   0.865   0.803
141   N         0.190   0.101   0.159   0.228   0.120   0.180  -0.009  -0.130  -0.057
142   E        -0.513  -0.558  -0.519  -0.259  -0.273  -0.216  -0.579  -0.624  -0.555
143   E        -0.677  -0.618  -0.582  -0.443  -0.329  -0.272  -0.768  -0.698  -0.631
144   F        -0.513  -0.522  -0.534  -0.862  -0.962  -0.931  -0.579  -0.593  -0.577
145   K         1.385   1.475   1.282   1.700   1.829   1.571   1.509   1.659   1.295
146   R        -0.513  -0.506  -0.518  -0.153  -0.125  -0.138  -0.579  -0.551  -0.552
147   L        -1.544  -1.506  -1.512  -1.383  -1.263  -1.306  -1.148  -1.107  -1.071
148   N        -1.111  -1.128  -1.066  -0.866  -0.869  -0.757  -0.958  -0.964  -0.870
149   Q        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
150   F        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
151   M        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
152   V        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
153   N        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
154   D        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
155   E        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
156   D        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
157   I        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
158   E        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
159   E         1.187   1.218   1.114   1.284   1.352   1.176   1.160   1.204   0.957
160   P         0.809   0.895   0.970   0.713   0.863   0.953   0.416   0.567   0.652
161   Q        -0.634  -0.548  -0.595  -0.518  -0.389  -0.439  -0.700  -0.589  -0.641
162   P         0.202   0.156   0.114   0.488   0.405   0.337   0.044  -0.033  -0.118
163   F         0.202   0.156   0.114   0.127   0.067  -0.006   0.044  -0.033  -0.118
164   T        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
165   V        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
166   K        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
167   T         0.006   0.051   0.091  -0.092  -0.017  -0.007  -0.258  -0.194  -0.154
168   E        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
169   T        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
170   T         0.006   0.037   0.054   0.099   0.153   0.148  -0.258  -0.216  -0.212
171   E        -0.438  -0.461  -0.486  -0.306  -0.302  -0.407  -0.452  -0.480  -0.505
172   I        -0.438  -0.370  -0.428  -0.254  -0.149  -0.224  -0.452  -0.359  -0.430
173   I         0.068   0.053   0.110  -0.100  -0.096  -0.037  -0.194  -0.204  -0.134
174   E        -0.628  -0.588  -0.540  -0.342  -0.252  -0.192  -0.710  -0.660  -0.584
175   I        -1.134  -1.125  -1.100  -1.319  -1.337  -1.319  -0.969  -0.951  -0.899
176   I        -1.134  -1.094  -1.052  -0.970  -0.920  -0.841  -0.969  -0.917  -0.851
177   N        -0.438  -0.454  -0.485  -0.229  -0.211  -0.303  -0.452  -0.471  -0.504
178   D        -1.134  -1.146  -1.091  -0.938  -0.943  -0.905  -0.969  -0.972  -0.891
179   E         0.383   0.372   0.279   0.731   0.745   0.587   0.323   0.300   0.145
180   E         0.202   0.171   0.169   0.309   0.284   0.250   0.044  -0.009  -0.031
181   N         0.006  -0.007   0.064  -0.006   0.007   0.079  -0.258  -0.284  -0.196
182   D        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
183   D        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
184   E        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
185   D        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
186   G        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
187   K        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
188   D        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
189   N        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
190   D        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
191   D        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
192   N        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
193   N        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
194   E        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
195   K        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
196   G        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
197   N        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
198   D        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
199   D        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
200   S        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
201   D        -0.752  -0.720  -0.630  -0.714  -0.662  -0.531  -0.839  -0.797  -0.681
202   A        -0.752  -0.720  -0.630  -1.019  -0.991  -0.848  -0.839  -0.797  -0.681
203   K        -0.752  -0.720  -0.630  -0.768  -0.730  -0.587  -0.839  -0.797  -0.681
204   K         0.006   0.125   0.111  -0.011   0.168   0.141  -0.258  -0.080  -0.122
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P47155
Output conservation file - output/P47155
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
