1     M         1.310   1.358   1.417   1.570   1.635   1.726   1.409   1.477   1.568
2     I         1.310   1.358   1.417   1.251   1.326   1.424   1.409   1.477   1.568
3     H        -0.021  -0.290  -0.389   0.433   0.053  -0.133  -0.007  -0.375  -0.522
4     A         0.113   0.208   0.245  -0.062   0.097   0.136  -0.225  -0.068  -0.043
5     V         0.628   0.479   0.353   0.621   0.423   0.228   0.646   0.406   0.159
6     L        -0.021  -0.058  -0.333  -0.157  -0.210  -0.605  -0.007  -0.067  -0.444
7     I         0.628   0.479   0.353   0.630   0.425   0.200   0.646   0.406   0.159
8     F         1.310   1.358   1.417   1.359   1.426   1.527   1.409   1.477   1.568
9     N         1.310   1.358   1.417   1.377   1.442   1.544   1.409   1.477   1.568
10    K        -0.453  -0.496  -0.439  -0.454  -0.504  -0.405  -0.661  -0.674  -0.596
11    K        -0.885  -0.909  -0.930  -0.712  -0.726  -0.806  -0.879  -0.881  -0.878
12    C         0.113   0.241   0.256   0.080   0.256   0.299  -0.225  -0.007  -0.021
13    Q        -0.453  -0.492  -0.432  -0.702  -0.695  -0.605  -0.661  -0.676  -0.588
14    P         1.310   1.358   1.417   1.508   1.568   1.667   1.409   1.477   1.568
15    R         1.310   1.358   1.417   1.480   1.542   1.641   1.409   1.477   1.568
16    L         1.310   1.358   1.417   1.056   1.128   1.239   1.409   1.477   1.568
17    V        -1.080  -0.871  -0.939  -1.091  -0.778  -0.894  -1.097  -0.853  -0.889
18    K         1.310   1.358   1.417   1.469   1.545   1.631   1.409   1.477   1.568
19    F         1.310   1.358   1.417   1.359   1.426   1.527   1.409   1.477   1.568
20    Y         1.310   1.358   1.417   1.518   1.587   1.677   1.409   1.477   1.568
21    T         0.628   0.683   0.377   0.780   0.853   0.418   0.646   0.725   0.202
22    P        -0.632  -0.617  -0.884  -0.301  -0.306  -0.754  -0.552  -0.543  -0.826
23    V        -0.021  -0.290  -0.389  -0.040  -0.469  -0.683  -0.007  -0.375  -0.522
24    D         0.292   0.425   0.305   0.292   0.519   0.271   0.102   0.316   0.071
25    L        -1.393  -1.453  -1.344  -1.620  -1.695  -1.553  -1.206  -1.237  -1.083
26    P        -0.962  -0.831  -0.917  -0.913  -0.749  -0.845  -0.988  -0.829  -0.870
27    K        -0.648  -0.630  -0.495  -0.778  -0.794  -0.573  -0.879  -0.847  -0.667
28    Q         1.310   1.358   1.417   1.359   1.443   1.527   1.409   1.477   1.568
29    K        -1.080  -0.879  -0.951  -1.005  -0.713  -0.803  -1.097  -0.858  -0.905
30    L        -1.198  -1.334  -1.337  -1.226  -1.393  -1.356  -0.988  -1.120  -1.074
31    L         1.310   1.358   1.417   1.056   1.128   1.239   1.409   1.477   1.568
32    L         0.292   0.168   0.226  -0.061  -0.298  -0.195   0.102  -0.143  -0.078
33    E        -1.393  -1.446  -1.347  -1.264  -1.354  -1.202  -1.206  -1.234  -1.085
34    Q        -0.021  -0.290  -0.389   0.163  -0.209  -0.405  -0.007  -0.375  -0.522
35    V         0.292   0.168   0.226   0.102  -0.147  -0.062   0.102  -0.143  -0.078
36    Y        -0.453  -0.516  -0.429  -0.268  -0.353  -0.224  -0.661  -0.706  -0.588
37    E        -1.393  -1.463  -1.359  -1.374  -1.466  -1.298  -1.206  -1.245  -1.095
38    L         0.628   0.514   0.371   0.459   0.313   0.117   0.646   0.463   0.190
39    I        -0.319  -0.397  -0.446  -0.502  -0.694  -0.790  -0.443  -0.582  -0.607
40    S        -0.021  -0.145  -0.367   0.088  -0.082  -0.452  -0.007  -0.184  -0.492
41    Q        -1.393  -1.413  -1.351  -1.311  -1.349  -1.256  -1.206  -1.207  -1.089
42    R         1.310   1.358   1.417   1.480   1.542   1.641   1.409   1.477   1.568
43    N        -1.080  -1.025  -0.971  -1.039  -0.936  -0.837  -1.097  -1.025  -0.924
44    S        -1.512  -1.476  -1.364  -1.375  -1.293  -1.129  -1.314  -1.255  -1.099
45    D        -1.393  -1.464  -1.347  -1.279  -1.379  -1.210  -1.206  -1.245  -1.085
46    F        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
47    Q        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
48    S        -0.571  -0.520  -0.455  -0.746  -0.667  -0.573  -0.770  -0.703  -0.616
49    S         0.113   0.241   0.256   0.104   0.349   0.316  -0.225  -0.007  -0.021
50    F        -1.080  -1.025  -0.971  -1.224  -1.141  -1.091  -1.097  -1.025  -0.924
51    L         0.113   0.241   0.256  -0.211   0.042   0.015  -0.225  -0.007  -0.021
52    V        -0.648  -0.526  -0.457  -1.181  -0.999  -0.875  -0.879  -0.712  -0.618
53    T        -0.632  -0.815  -0.913  -0.389  -0.669  -0.830  -0.552  -0.773  -0.858
54    P         0.568   0.357   0.313   0.785   0.414   0.283   0.555   0.200   0.084
55    P         0.628   0.514   0.371   0.898   0.736   0.493   0.646   0.463   0.190
56    S        -0.962  -0.949  -0.927  -0.893  -0.852  -0.804  -0.988  -0.949  -0.881
57    L         0.628   0.738   0.387   0.434   0.583   0.082   0.646   0.804   0.220
58    L         0.628   0.514   0.371   0.438   0.286   0.065   0.646   0.463   0.190
59    L        -1.514  -1.311  -1.354  -1.486  -1.171  -1.259  -1.302  -1.086  -1.090
60    S        -1.514  -1.379  -1.359  -1.459  -1.279  -1.241  -1.302  -1.162  -1.093
61    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
62    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
63    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
64    N        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
65    E        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
66    N        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
67    N        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
68    N        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
69    D        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
70    E        -1.100  -0.965  -0.953  -1.250  -1.019  -1.033  -1.101  -0.939  -0.903
71    V        -0.131   0.081  -0.345  -0.078   0.217  -0.429  -0.156   0.133  -0.462
72    N        -0.131  -0.219  -0.398   0.089  -0.043  -0.308  -0.156  -0.308  -0.536
73    N        -1.494  -1.497  -1.377  -1.591  -1.544  -1.353  -1.294  -1.273  -1.109
74    E        -0.507  -0.595  -0.469  -0.638  -0.732  -0.548  -0.725  -0.805  -0.635
75    D         0.568   0.585   0.345   0.719   0.760   0.375   0.555   0.575   0.144
76    I         0.568   0.357   0.313   0.553   0.231   0.123   0.555   0.200   0.084
77    Q        -1.080  -1.116  -0.974  -0.973  -0.996  -0.778  -1.097  -1.106  -0.926
78    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
79    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
80    I         0.056   0.102   0.211  -0.350  -0.282  -0.092  -0.334  -0.269  -0.107
81    I         0.628   0.479   0.353   0.653   0.460   0.261   0.646   0.406   0.159
82    Y         1.310   1.358   1.417   1.518   1.587   1.677   1.409   1.477   1.568
83    K         0.056   0.119   0.214  -0.002   0.094   0.244  -0.334  -0.235  -0.102
84    N        -0.453  -0.492  -0.432  -0.378  -0.426  -0.330  -0.661  -0.676  -0.588
85    Y         1.310   1.358   1.417   1.518   1.587   1.677   1.409   1.477   1.568
86    A         1.310   1.358   1.417   1.384   1.444   1.551   1.409   1.477   1.568
87    T         1.310   1.358   1.417   1.377   1.443   1.544   1.409   1.477   1.568
88    L         1.310   1.358   1.417   1.056   1.128   1.239   1.409   1.477   1.568
89    Y         1.310   1.358   1.417   1.518   1.587   1.677   1.409   1.477   1.568
90    F         1.310   1.358   1.417   1.359   1.426   1.527   1.409   1.477   1.568
91    T        -0.453  -0.496  -0.439  -0.665  -0.718  -0.623  -0.661  -0.674  -0.596
92    F         0.628   0.479   0.353   0.744   0.532   0.310   0.646   0.406   0.159
93    I         0.292   0.252   0.247   0.122   0.034  -0.012   0.102   0.013  -0.039
94    V         0.628   0.479   0.353   0.612   0.412   0.203   0.646   0.406   0.159
95    D         0.628   0.479   0.353   0.804   0.620   0.396   0.646   0.406   0.159
96    D        -0.453  -0.541  -0.440  -0.559  -0.679  -0.537  -0.661  -0.744  -0.601
97    Q        -0.021  -0.290  -0.389   0.174  -0.204  -0.379  -0.007  -0.375  -0.522
98    E         1.310   1.358   1.417   1.291   1.368   1.462   1.409   1.477   1.568
99    S         1.310   1.358   1.417   1.298   1.379   1.469   1.409   1.477   1.568
100   E         0.628   0.479   0.353   0.712   0.531   0.348   0.646   0.406   0.159
101   L         1.310   1.358   1.417   1.056   1.128   1.239   1.409   1.477   1.568
102   A         0.056   0.101   0.210  -0.134  -0.096   0.112  -0.334  -0.271  -0.108
103   I         0.628   0.479   0.353   0.607   0.387   0.136   0.646   0.406   0.159
104   L         0.628   0.479   0.353   0.438   0.234   0.034   0.646   0.406   0.159
105   D         1.310   1.358   1.417   1.402   1.488   1.567   1.409   1.477   1.568
106   L         1.310   1.358   1.417   1.056   1.128   1.239   1.409   1.477   1.568
107   I         1.310   1.358   1.417   1.251   1.326   1.424   1.409   1.477   1.568
108   Q         1.310   1.358   1.417   1.359   1.443   1.527   1.409   1.477   1.568
109   T         0.113   0.241   0.256  -0.165   0.045   0.066  -0.225  -0.007  -0.021
110   F        -0.021  -0.145  -0.367   0.229   0.062  -0.216  -0.007  -0.184  -0.492
111   V         1.310   1.358   1.417   1.221   1.293   1.396   1.409   1.477   1.568
112   E         1.310   1.358   1.417   1.291   1.368   1.462   1.409   1.477   1.568
113   S        -0.648  -0.621  -0.492  -0.682  -0.669  -0.480  -0.879  -0.835  -0.663
114   L         1.310   1.358   1.417   1.056   1.128   1.239   1.409   1.477   1.568
115   D         1.310   1.358   1.417   1.402   1.488   1.567   1.409   1.477   1.568
116   R         0.113   0.236   0.245   0.110   0.309   0.305  -0.225  -0.017  -0.042
117   C         0.628   0.479   0.353   0.932   0.707   0.432   0.646   0.406   0.159
118   F         1.310   1.358   1.417   1.359   1.426   1.527   1.409   1.477   1.568
119   T        -1.080  -1.038  -0.948  -1.195  -1.105  -0.980  -1.097  -1.018  -0.901
120   E         0.113   0.241   0.256  -0.073   0.154   0.150  -0.225  -0.007  -0.021
121   V         1.310   1.358   1.417   1.221   1.293   1.396   1.409   1.477   1.568
122   N         0.113   0.241   0.256   0.144   0.373   0.355  -0.225  -0.007  -0.021
123   E         1.310   1.358   1.417   1.291   1.368   1.462   1.409   1.477   1.568
124   L         1.310   1.358   1.417   1.056   1.128   1.239   1.409   1.477   1.568
125   D         1.310   1.358   1.417   1.402   1.488   1.567   1.409   1.477   1.568
126   L         1.310   1.358   1.417   1.056   1.128   1.239   1.409   1.477   1.568
127   I         0.056   0.101   0.210  -0.350  -0.284  -0.093  -0.334  -0.271  -0.108
128   F         1.310   1.358   1.417   1.359   1.426   1.527   1.409   1.477   1.568
129   N        -0.453  -0.492  -0.432  -0.490  -0.532  -0.412  -0.661  -0.676  -0.588
130   W         0.292   0.168   0.226   0.514   0.255   0.306   0.102  -0.143  -0.078
131   Q         0.628   0.479   0.353   0.749   0.559   0.325   0.646   0.406   0.159
132   T        -0.319  -0.328  -0.374  -0.335  -0.328  -0.438  -0.443  -0.430  -0.502
133   L         0.628   0.514   0.371   0.438   0.286   0.065   0.646   0.463   0.190
134   E        -0.571  -0.560  -0.467  -0.627  -0.570  -0.439  -0.770  -0.763  -0.631
135   S        -0.648  -0.630  -0.495  -0.834  -0.810  -0.591  -0.879  -0.847  -0.667
136   V        -0.021  -0.290  -0.389   0.014  -0.376  -0.546  -0.007  -0.375  -0.522
137   L         1.310   1.358   1.417   1.056   1.128   1.239   1.409   1.477   1.568
138   E        -0.319  -0.477  -0.445  -0.339  -0.596  -0.527  -0.443  -0.653  -0.607
139   E         1.310   1.358   1.417   1.291   1.368   1.462   1.409   1.477   1.568
140   I        -0.021  -0.290  -0.389   0.072  -0.295  -0.451  -0.007  -0.375  -0.522
141   V         0.056   0.102   0.212  -0.350  -0.279  -0.089  -0.334  -0.268  -0.105
142   Q        -0.021  -0.290  -0.389   0.135  -0.259  -0.475  -0.007  -0.375  -0.522
143   G         1.310   1.358   1.417   1.381   1.428   1.547   1.409   1.477   1.568
144   G         1.310   1.358   1.417   1.381   1.428   1.547   1.409   1.477   1.568
145   M        -0.319  -0.467  -0.436  -0.221  -0.511  -0.553  -0.443  -0.631  -0.594
146   V         1.310   1.358   1.417   1.221   1.293   1.396   1.409   1.477   1.568
147   I        -0.021  -0.114  -0.352  -0.015  -0.167  -0.594  -0.007  -0.138  -0.470
148   E         0.292   0.433   0.326   0.220   0.444   0.260   0.102   0.329   0.109
149   T         1.310   1.358   1.417   1.377   1.443   1.544   1.409   1.477   1.568
150   N         0.292   0.379   0.263   0.270   0.401   0.168   0.102   0.237  -0.009
151   V        -0.885  -1.018  -0.936  -1.013  -1.196  -1.081  -0.879  -0.995  -0.885
152   N        -1.707  -1.636  -1.643  -1.460  -1.394  -1.392  -1.314  -1.239  -1.183
153   R        -0.453  -0.469  -0.414  -0.392  -0.393  -0.325  -0.661  -0.640  -0.566
154   I         1.310   1.358   1.417   1.251   1.326   1.424   1.409   1.477   1.568
155   V         1.310   1.358   1.417   1.221   1.293   1.396   1.409   1.477   1.568
156   A        -1.080  -1.104  -0.963  -1.213  -1.276  -1.066  -1.097  -1.095  -0.917
157   S        -0.319  -0.349  -0.398  -0.282  -0.317  -0.413  -0.443  -0.458  -0.537
158   V         0.628   0.514   0.371   0.624   0.478   0.267   0.646   0.463   0.190
159   D        -0.021  -0.290  -0.389   0.230  -0.129  -0.312  -0.007  -0.375  -0.522
160   E        -0.632  -0.815  -0.913  -0.461  -0.737  -0.870  -0.552  -0.773  -0.858
161   L        -0.648  -0.630  -0.495  -1.005  -0.950  -0.738  -0.879  -0.847  -0.667
162   N        -0.021  -0.290  -0.389   0.224  -0.154  -0.287  -0.007  -0.375  -0.522
163   K        -1.825  -1.836  -1.695  -1.551  -1.560  -1.336  -1.423  -1.402  -1.223
164   A        -1.393  -1.483  -1.365  -1.276  -1.425  -1.235  -1.206  -1.257  -1.099
165   A        -1.512  -1.481  -1.352  -1.322  -1.304  -1.074  -1.314  -1.261  -1.089
166   E        -1.393  -1.285  -1.350  -1.309  -1.138  -1.226  -1.206  -1.100  -1.087
167   S        -0.319  -0.338  -0.435  -0.324  -0.387  -0.508  -0.443  -0.522  -0.592
168   T        -2.139  -2.097  -1.965  -1.865  -1.819  -1.625  -1.532  -1.477  -1.304
169   D        -1.825  -1.765  -1.674  -1.725  -1.663  -1.526  -1.423  -1.352  -1.208
170   S        -1.494  -1.389  -1.352  -1.377  -1.217  -1.135  -1.294  -1.175  -1.088
171   K        -0.409  -0.435  -0.442  -0.330  -0.407  -0.452  -0.543  -0.594  -0.596
172   I        -1.602  -1.508  -1.385  -1.795  -1.650  -1.464  -1.380  -1.276  -1.115
173   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
174   G        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
175   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
176   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
177   R        -1.100  -0.981  -0.956  -1.079  -0.899  -0.893  -1.101  -0.957  -0.905
178   L         0.092   0.101   0.212  -0.338  -0.336  -0.104  -0.264  -0.270  -0.106
179   T        -1.100  -0.974  -0.954  -1.125  -0.964  -0.914  -1.101  -0.949  -0.903
180   S        -0.885  -0.769  -0.912  -0.795  -0.692  -0.846  -0.879  -0.768  -0.863
181   T        -1.512  -1.350  -1.353  -1.339  -1.079  -1.092  -1.314  -1.153  -1.092
182   G        -1.852  -1.766  -1.670  -1.598  -1.495  -1.341  -1.437  -1.344  -1.204
183   F        -0.642  -0.600  -0.491  -1.011  -0.981  -0.806  -0.868  -0.809  -0.662
184   G        -0.453  -0.366  -0.422  -0.573  -0.474  -0.492  -0.661  -0.561  -0.580
185   S        -1.707  -1.604  -1.649  -1.531  -1.396  -1.456  -1.314  -1.209  -1.188
186   A        -0.885  -0.929  -0.933  -0.794  -0.909  -0.882  -0.879  -0.931  -0.887
187   L        -0.962  -0.838  -0.921  -1.210  -1.069  -1.125  -0.988  -0.841  -0.874
188   Q        -1.393  -1.483  -1.365  -1.469  -1.591  -1.409  -1.206  -1.257  -1.099
189   A        -0.319  -0.258  -0.401  -0.342  -0.280  -0.541  -0.443  -0.380  -0.547
190   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
191   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
192   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
193   F        -0.453  -0.412  -0.438  -0.680  -0.646  -0.601  -0.661  -0.608  -0.598
194   A        -0.453  -0.499  -0.430  -0.552  -0.594  -0.484  -0.661  -0.679  -0.586
195   Q        -1.852  -1.756  -1.688  -1.469  -1.313  -1.244  -1.437  -1.342  -1.217
196   G        -1.001  -0.861  -0.941  -1.035  -0.854  -0.946  -1.010  -0.848  -0.893
197   G        -0.131   0.042  -0.347   0.064   0.257  -0.252  -0.156   0.081  -0.463
198   F        -1.001  -1.079  -0.975  -0.935  -1.069  -0.871  -1.010  -1.071  -0.926
199   A        -0.520  -0.377  -0.451  -0.607  -0.380  -0.516  -0.721  -0.523  -0.609
200   Q        -1.602  -1.503  -1.385  -1.693  -1.570  -1.368  -1.380  -1.272  -1.115
201   W         1.310   1.358   1.417   1.606   1.680   1.761   1.409   1.477   1.568
202   A        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
203   T        -1.001  -0.907  -0.921  -0.991  -0.869  -0.850  -1.010  -0.901  -0.873
204   G        -1.393  -1.281  -1.315  -1.122  -1.009  -0.982  -1.206  -1.092  -1.059
205   Q        -0.962  -0.947  -0.931  -0.876  -0.843  -0.798  -0.988  -0.949  -0.883
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P47064
Output conservation file - output/P47064
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
