1     M         0.995   1.013   1.059   1.327   1.337   1.375   1.044   1.072   1.139
2     A        -1.049  -0.908  -0.984  -1.248  -0.911  -1.079  -1.157  -0.926  -1.005
3     R         0.995   1.013   1.059   0.790   0.831   0.924   1.044   1.072   1.139
4     Y         0.995   1.013   1.059   1.202   1.227   1.270   1.044   1.072   1.139
5     G        -1.402  -1.240  -1.035  -1.801  -1.594  -1.260  -1.540  -1.343  -1.067
6     A         0.234   0.145  -0.175  -0.048  -0.135  -0.522   0.278   0.152  -0.364
7     T        -1.193  -1.288  -1.549  -0.825  -0.968  -1.495  -0.966  -1.070  -1.312
8     S        -1.049  -1.142  -1.020  -0.962  -1.060  -0.884  -1.157  -1.233  -1.051
9     T        -1.656  -1.692  -1.569  -1.837  -1.853  -1.587  -1.540  -1.515  -1.331
10    N        -1.512  -1.706  -1.583  -1.204  -1.560  -1.424  -1.349  -1.531  -1.344
11    P        -0.161  -0.156  -0.190   0.081   0.062  -0.045  -0.296  -0.302  -0.389
12    A        -0.394  -0.389  -0.266  -0.883  -0.859  -0.602  -0.679  -0.693  -0.518
13    K         0.995   1.013   1.059   0.870   0.915   0.991   1.044   1.072   1.139
14    S        -0.857  -0.930  -0.993  -0.622  -0.701  -0.793  -0.870  -0.958  -1.015
15    A         0.995   1.013   1.059   0.567   0.613   0.737   1.044   1.072   1.139
16    S        -0.857  -1.026  -1.003  -0.830  -1.089  -1.138  -0.870  -1.070  -1.028
17    A         0.234   0.023  -0.184  -0.055  -0.298  -0.558   0.278  -0.025  -0.378
18    R         0.995   1.013   1.059   0.790   0.831   0.924   1.044   1.072   1.139
19    G         0.995   1.013   1.059   1.063   1.085   1.154   1.044   1.072   1.139
20    S        -0.394  -0.389  -0.266  -0.744  -0.784  -0.628  -0.679  -0.693  -0.518
21    Y         0.995   1.013   1.059   1.202   1.227   1.270   1.044   1.072   1.139
22    L         0.995   1.013   1.059   1.025   1.050   1.121   1.044   1.072   1.139
23    R         0.995   1.013   1.059   0.790   0.831   0.924   1.044   1.072   1.139
24    V        -0.394  -0.389  -0.266  -0.465  -0.481  -0.306  -0.679  -0.693  -0.518
25    S        -0.161  -0.156  -0.190  -0.098  -0.082  -0.147  -0.296  -0.302  -0.389
26    F        -0.161   0.016  -0.211   0.131   0.356  -0.014  -0.296  -0.035  -0.425
27    K         0.995   1.013   1.059   0.870   0.915   0.991   1.044   1.072   1.139
28    N         0.995   1.013   1.059   1.116   1.142   1.198   1.044   1.072   1.139
29    T        -0.161  -0.156  -0.190  -0.074  -0.074  -0.183  -0.296  -0.302  -0.389
30    R         0.234   0.145  -0.175   0.200   0.117  -0.273   0.278   0.152  -0.364
31    E         0.995   1.013   1.059   1.056   1.079   1.148   1.044   1.072   1.139
32    T        -0.394  -0.389  -0.266  -0.443  -0.465  -0.301  -0.679  -0.693  -0.518
33    A         0.995   1.013   1.059   0.567   0.613   0.737   1.044   1.072   1.139
34    Q        -0.857  -0.930  -0.993  -0.595  -0.695  -0.805  -0.870  -0.958  -1.015
35    A        -0.161  -0.156  -0.190  -0.540  -0.523  -0.521  -0.296  -0.302  -0.389
36    I         0.995   1.013   1.059   1.116   1.139   1.198   1.044   1.072   1.139
37    N        -0.857  -0.785  -0.985  -0.653  -0.546  -0.953  -0.870  -0.759  -1.006
38    G         0.995   1.013   1.059   1.063   1.085   1.154   1.044   1.072   1.139
39    W        -0.161  -0.156  -0.190   0.268   0.251   0.150  -0.296  -0.302  -0.389
40    E        -1.865  -1.664  -1.558  -1.825  -1.561  -1.391  -1.732  -1.513  -1.323
41    L         0.995   1.013   1.059   1.025   1.050   1.121   1.044   1.072   1.139
42    T        -1.992  -1.738  -1.564  -1.954  -1.610  -1.373  -1.827  -1.562  -1.326
43    K        -0.161  -0.228  -0.254  -0.321  -0.440  -0.540  -0.296  -0.420  -0.498
44    A         0.995   1.013   1.059   0.567   0.613   0.737   1.044   1.072   1.139
45    Q        -0.857  -0.930  -0.993  -0.601  -0.703  -0.820  -0.870  -0.958  -1.015
46    K        -0.857  -0.930  -0.993  -0.879  -0.996  -1.170  -0.870  -0.958  -1.015
47    Y        -0.394  -0.417  -0.269  -0.236  -0.307  -0.115  -0.679  -0.740  -0.525
48    L         0.995   1.013   1.059   1.025   1.050   1.121   1.044   1.072   1.139
49    D         0.234   0.023  -0.184   0.644   0.368   0.003   0.278  -0.025  -0.378
50    Q        -0.394  -0.417  -0.269  -0.419  -0.477  -0.267  -0.679  -0.740  -0.525
51    V         0.995   1.013   1.059   1.021   1.049   1.118   1.044   1.072   1.139
52    L        -0.857  -0.930  -0.993  -0.728  -0.844  -0.961  -0.870  -0.958  -1.015
53    D        -0.504  -0.439  -0.269  -0.484  -0.426  -0.183  -0.870  -0.780  -0.525
54    H         0.234   0.023  -0.184   0.605   0.320  -0.082   0.278  -0.025  -0.378
55    Q        -0.504  -0.417  -0.255  -0.765  -0.652  -0.402  -0.870  -0.742  -0.500
56    R        -0.857  -1.026  -1.003  -0.901  -1.118  -1.063  -0.870  -1.070  -1.028
57    A         0.995   1.013   1.059   0.567   0.613   0.737   1.044   1.072   1.139
58    I        -0.394  -0.417  -0.269  -0.401  -0.489  -0.281  -0.679  -0.740  -0.525
59    P         0.995   1.013   1.059   1.205   1.223   1.273   1.044   1.072   1.139
60    F         0.234   0.023  -0.184   0.640   0.389   0.082   0.278  -0.025  -0.378
61    R         0.995   1.013   1.059   0.790   0.831   0.924   1.044   1.072   1.139
62    R         0.234   0.145  -0.175   0.157   0.062  -0.412   0.278   0.152  -0.364
63    F        -1.049  -1.067  -1.011  -0.723  -0.748  -0.671  -1.157  -1.157  -1.041
64    N        -0.161  -0.310  -0.265  -0.136  -0.470  -0.520  -0.296  -0.556  -0.517
65    S        -0.504  -0.417  -0.255  -0.707  -0.555  -0.313  -0.870  -0.742  -0.500
66    S        -0.394  -0.417  -0.269  -0.517  -0.569  -0.349  -0.679  -0.740  -0.525
67    I        -0.857  -1.026  -1.003  -0.593  -0.842  -0.855  -0.870  -1.070  -1.028
68    G         0.995   1.013   1.059   1.063   1.085   1.154   1.044   1.072   1.139
69    R         0.995   1.013   1.059   0.790   0.831   0.924   1.044   1.072   1.139
70    T         0.234   0.023  -0.184   0.403   0.107  -0.330   0.278  -0.025  -0.378
71    A        -0.394  -0.328  -0.261  -0.886  -0.784  -0.605  -0.679  -0.587  -0.510
72    Q         0.995   1.013   1.059   1.049   1.085   1.142   1.044   1.072   1.139
73    G         0.995   1.013   1.059   1.063   1.085   1.154   1.044   1.072   1.139
74    K         0.995   1.013   1.059   0.870   0.915   0.991   1.044   1.072   1.139
75    E         0.234   0.023  -0.184   0.447   0.182  -0.148   0.278  -0.025  -0.378
76    F         0.995   1.013   1.059   1.216   1.234   1.282   1.044   1.072   1.139
77    G         0.995   1.013   1.059   1.063   1.085   1.154   1.044   1.072   1.139
78    V         0.995   1.013   1.059   1.021   1.049   1.118   1.044   1.072   1.139
79    T         0.234   0.023  -0.184   0.451   0.191  -0.170   0.278  -0.025  -0.378
80    K        -0.857  -1.026  -1.003  -0.866  -1.114  -1.125  -0.870  -1.070  -1.028
81    A         0.995   1.013   1.059   0.567   0.613   0.737   1.044   1.072   1.139
82    R         0.995   1.013   1.059   0.790   0.831   0.924   1.044   1.072   1.139
83    W         0.995   1.013   1.059   1.324   1.340   1.373   1.044   1.072   1.139
84    P         0.995   1.013   1.059   1.205   1.223   1.273   1.044   1.072   1.139
85    A        -0.394  -0.389  -0.266  -0.908  -0.892  -0.632  -0.679  -0.693  -0.518
86    K         0.995   1.013   1.059   0.870   0.915   0.991   1.044   1.072   1.139
87    S         0.995   1.013   1.059   0.968   1.015   1.073   1.044   1.072   1.139
88    V        -0.497  -0.779  -0.986  -0.248  -0.665  -1.050  -0.392  -0.751  -1.006
89    K        -1.049  -1.122  -1.005  -1.095  -1.161  -0.972  -1.157  -1.204  -1.035
90    F         0.995   1.013   1.059   1.216   1.234   1.282   1.044   1.072   1.139
91    V        -1.783  -1.762  -1.574  -1.526  -1.511  -1.265  -1.636  -1.584  -1.337
92    Q        -1.783  -1.793  -1.585  -1.610  -1.614  -1.375  -1.636  -1.614  -1.346
93    G        -0.504  -0.409  -0.263  -0.480  -0.344  -0.151  -0.870  -0.727  -0.513
94    L         0.995   1.013   1.059   1.025   1.050   1.121   1.044   1.072   1.139
95    L         0.995   1.013   1.059   1.025   1.050   1.121   1.044   1.072   1.139
96    Q        -1.320  -1.184  -1.039  -1.541  -1.361  -1.171  -1.445  -1.284  -1.071
97    N         0.995   1.013   1.059   1.116   1.142   1.198   1.044   1.072   1.139
98    A         0.234   0.338  -0.159  -0.042   0.092  -0.479   0.278   0.413  -0.336
99    A        -1.402  -1.229  -1.035  -1.751  -1.403  -1.144  -1.540  -1.329  -1.067
100   A         0.234   0.408  -0.155  -0.055   0.157  -0.515   0.278   0.501  -0.330
101   N         0.995   1.013   1.059   1.116   1.142   1.198   1.044   1.072   1.139
102   A         0.995   1.013   1.059   0.567   0.613   0.737   1.044   1.072   1.139
103   E         0.234   0.023  -0.184   0.472   0.223  -0.065   0.278  -0.025  -0.378
104   A        -0.497  -0.496  -0.958  -0.658  -0.644  -1.193  -0.392  -0.401  -0.972
105   K         0.995   1.013   1.059   0.870   0.915   0.991   1.044   1.072   1.139
106   G         0.995   1.013   1.059   1.063   1.085   1.154   1.044   1.072   1.139
107   L         0.995   1.013   1.059   1.025   1.050   1.121   1.044   1.072   1.139
108   D         0.995   1.013   1.059   1.253   1.269   1.313   1.044   1.072   1.139
109   A        -2.119  -2.179  -2.019  -2.095  -2.119  -1.863  -1.732  -1.764  -1.520
110   T        -2.328  -2.323  -2.026  -1.958  -1.940  -1.587  -1.923  -1.867  -1.525
111   K        -1.193  -1.311  -1.549  -0.963  -1.118  -1.474  -0.966  -1.119  -1.312
112   L         0.995   1.013   1.059   1.025   1.050   1.121   1.044   1.072   1.139
113   Y        -1.865  -1.665  -1.561  -1.662  -1.429  -1.306  -1.732  -1.513  -1.323
114   V        -0.504  -0.438  -0.270  -0.631  -0.541  -0.283  -0.870  -0.779  -0.527
115   S        -0.394  -0.417  -0.269  -0.606  -0.674  -0.464  -0.679  -0.740  -0.525
116   H         0.995   1.013   1.059   1.236   1.254   1.299   1.044   1.072   1.139
117   I        -0.161  -0.156  -0.190  -0.015  -0.028  -0.126  -0.296  -0.302  -0.389
118   Q         0.995   1.013   1.059   1.049   1.085   1.142   1.044   1.072   1.139
119   V         0.995   1.013   1.059   1.021   1.049   1.118   1.044   1.072   1.139
120   N         0.995   1.013   1.059   1.116   1.142   1.198   1.044   1.072   1.139
121   Q        -1.176  -0.997  -0.995  -1.190  -0.972  -0.975  -1.253  -1.035  -1.018
122   A         0.995   1.013   1.059   0.567   0.613   0.737   1.044   1.072   1.139
123   P         0.995   1.013   1.059   1.205   1.223   1.273   1.044   1.072   1.139
124   K         0.995   1.013   1.059   0.870   0.915   0.991   1.044   1.072   1.139
125   Q         0.995   1.013   1.059   1.049   1.085   1.142   1.044   1.072   1.139
126   R         0.995   1.013   1.059   0.790   0.831   0.924   1.044   1.072   1.139
127   R         0.995   1.013   1.059   0.790   0.831   0.924   1.044   1.072   1.139
128   R         0.995   1.013   1.059   0.790   0.831   0.924   1.044   1.072   1.139
129   T         0.995   1.013   1.059   1.070   1.103   1.159   1.044   1.072   1.139
130   Y         0.234   0.408  -0.155   0.612   0.794   0.073   0.278   0.501  -0.330
131   R         0.995   1.013   1.059   0.790   0.831   0.924   1.044   1.072   1.139
132   A         0.995   1.013   1.059   0.567   0.613   0.737   1.044   1.072   1.139
133   H         0.995   1.013   1.059   1.236   1.254   1.299   1.044   1.072   1.139
134   G         0.995   1.013   1.059   1.063   1.085   1.154   1.044   1.072   1.139
135   R         0.995   1.013   1.059   0.790   0.831   0.924   1.044   1.072   1.139
136   I        -0.161  -0.156  -0.190  -0.015  -0.028  -0.126  -0.296  -0.302  -0.389
137   N        -0.161  -0.156  -0.190  -0.002  -0.009  -0.106  -0.296  -0.302  -0.389
138   K        -1.049  -1.005  -0.989  -1.478  -1.318  -1.217  -1.157  -1.041  -1.011
139   Y         0.995   1.013   1.059   1.202   1.227   1.270   1.044   1.072   1.139
140   E        -1.865  -1.839  -1.580  -1.680  -1.601  -1.238  -1.732  -1.659  -1.341
141   S         0.995   1.013   1.059   0.968   1.015   1.073   1.044   1.072   1.139
142   S        -0.497  -0.590  -0.969  -0.232  -0.347  -0.862  -0.392  -0.532  -0.985
143   P         0.995   1.013   1.059   1.205   1.223   1.273   1.044   1.072   1.139
144   S        -0.161  -0.228  -0.254  -0.061  -0.140  -0.165  -0.296  -0.420  -0.498
145   H         0.995   1.013   1.059   1.236   1.254   1.299   1.044   1.072   1.139
146   I         0.995   1.013   1.059   1.116   1.139   1.198   1.044   1.072   1.139
147   E         0.995   1.013   1.059   1.056   1.079   1.148   1.044   1.072   1.139
148   L        -0.161  -0.156  -0.190  -0.078  -0.086  -0.156  -0.296  -0.302  -0.389
149   V        -1.049  -0.727  -0.967  -0.929  -0.456  -0.796  -1.157  -0.728  -0.983
150   V        -0.504  -0.438  -0.266  -0.689  -0.602  -0.332  -0.870  -0.777  -0.520
151   T        -0.857  -0.930  -0.993  -0.794  -0.925  -1.096  -0.870  -0.958  -1.015
152   E         0.995   1.013   1.059   1.056   1.079   1.148   1.044   1.072   1.139
153   K        -1.656  -1.692  -1.569  -1.628  -1.667  -1.520  -1.540  -1.515  -1.331
154   E        -1.512  -1.535  -1.570  -1.199  -1.296  -1.366  -1.349  -1.381  -1.333
155   E         0.995   1.013   1.059   1.056   1.079   1.148   1.044   1.072   1.139
156   A        -1.193  -1.351  -1.553  -1.234  -1.453  -1.586  -0.966  -1.156  -1.315
157   V         0.234   0.145  -0.175   0.420   0.318  -0.134   0.278   0.152  -0.364
158   A        -1.865  -1.858  -1.584  -1.979  -1.929  -1.526  -1.732  -1.678  -1.346
159   K         0.995   1.013   1.059   0.870   0.915   0.991   1.044   1.072   1.139
160   A        -0.497  -0.590  -0.969  -0.636  -0.764  -1.146  -0.392  -0.532  -0.985
161   A        -3.127  -2.925  -2.675  -2.883  -2.552  -2.234  -2.210  -2.030  -1.717
162   E        -1.049  -1.145  -1.020  -0.941  -1.121  -0.984  -1.157  -1.237  -1.050
163   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
164   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
165   K        -2.224  -2.235  -2.027  -2.128  -2.236  -1.916  -1.804  -1.806  -1.526
166   K        -1.512  -1.706  -1.583  -1.385  -1.641  -1.468  -1.349  -1.531  -1.344
167   V        -2.119  -2.045  -2.002  -1.811  -1.751  -1.707  -1.732  -1.652  -1.507
168   V        -1.865  -1.668  -1.562  -2.108  -1.902  -1.627  -1.732  -1.521  -1.325
169   R        -0.161  -0.310  -0.265  -0.271  -0.473  -0.358  -0.296  -0.556  -0.517
170   L        -0.857  -0.930  -0.993  -0.673  -0.786  -0.880  -0.870  -0.958  -1.015
171   T        -0.857  -0.541  -0.965  -0.704  -0.307  -0.856  -0.870  -0.493  -0.981
172   S        -0.857  -0.769  -0.973  -0.742  -0.640  -0.924  -0.870  -0.782  -0.990
173   R        -0.161  -0.156  -0.190  -0.378  -0.371  -0.439  -0.296  -0.302  -0.389
174   Q         0.995   1.013   1.059   1.049   1.085   1.142   1.044   1.072   1.139
175   R        -0.161  -0.156  -0.190  -0.322  -0.315  -0.354  -0.296  -0.302  -0.389
176   G        -0.161  -0.156  -0.190  -0.188  -0.213  -0.351  -0.296  -0.302  -0.389
177   R        -0.857  -1.026  -1.003  -1.044  -1.333  -1.367  -0.870  -1.070  -1.028
178   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
179   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
180   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
181   I        -0.449  -0.352  -0.260  -0.533  -0.398  -0.270  -0.773  -0.629  -0.509
182   A         0.234   0.023  -0.184  -0.079  -0.342  -0.639   0.278  -0.025  -0.378
183   A        -1.783  -1.660  -1.570  -2.053  -1.865  -1.652  -1.636  -1.473  -1.333
184   Q        -0.394  -0.417  -0.269  -0.566  -0.665  -0.458  -0.679  -0.740  -0.525
185   K         0.234   0.023  -0.184   0.228  -0.036  -0.398   0.278  -0.025  -0.378
186   R        -0.394  -0.417  -0.269  -0.917  -1.025  -0.769  -0.679  -0.740  -0.525
187   I        -0.497  -0.295  -0.946  -0.105   0.118  -0.775  -0.392  -0.151  -0.956
188   S        -0.161  -0.094  -0.236  -0.074   0.022  -0.269  -0.296  -0.204  -0.467
189   -        -0.097   0.032  -0.197  -0.473  -0.283  -0.561  -0.197  -0.012  -0.401
190   A         0.995   1.013   1.059   0.567   0.613   0.737   1.044   1.072   1.139
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P46990
Output conservation file - output/P46990
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
