1     M         1.445   1.452   1.510   1.655   1.664   1.752   1.567   1.577   1.664
2     F        -1.247  -1.211  -1.174  -1.243  -1.189  -1.141  -1.083  -1.048  -0.970
3     R        -0.869  -0.856  -0.800  -0.806  -0.785  -0.701  -0.893  -0.876  -0.797
4     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
5     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
6     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
7     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
8     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
9     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
10    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
11    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
12    A        -0.865  -0.849  -0.801  -0.725  -0.714  -0.636  -0.901  -0.881  -0.801
13    G        -0.312  -0.335  -0.302  -0.508  -0.556  -0.498  -0.521  -0.555  -0.499
14    S        -0.713  -0.721  -0.748  -0.674  -0.678  -0.732  -0.711  -0.719  -0.739
15    S        -1.936  -1.919  -1.789  -1.822  -1.802  -1.611  -1.362  -1.345  -1.197
16    K        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
17    R        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
18    F        -0.903  -0.892  -0.812  -0.934  -0.918  -0.784  -0.943  -0.929  -0.813
19    L        -0.559  -0.560  -0.706  -0.559  -0.565  -0.808  -0.525  -0.528  -0.691
20    R         0.406   0.386   0.392   0.336   0.294   0.276   0.172   0.134   0.105
21    N        -0.773  -0.782  -0.778  -0.750  -0.754  -0.745  -0.804  -0.814  -0.779
22    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
23    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
24    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
25    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
26    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
27    T        -0.429  -0.435  -0.348  -0.589  -0.615  -0.476  -0.664  -0.673  -0.556
28    L        -0.300  -0.319  -0.292  -0.610  -0.638  -0.558  -0.525  -0.541  -0.487
29    I        -0.559  -0.588  -0.692  -0.542  -0.590  -0.798  -0.525  -0.560  -0.674
30    A        -1.118  -1.161  -1.163  -0.976  -1.044  -1.061  -0.943  -0.986  -0.958
31    I        -1.591  -1.599  -1.518  -1.711  -1.719  -1.614  -1.222  -1.225  -1.111
32    S        -0.773  -0.794  -0.783  -0.823  -0.842  -0.834  -0.804  -0.817  -0.782
33    W         1.445   1.452   1.510   1.631   1.641   1.729   1.567   1.577   1.664
34    V        -0.903  -0.892  -0.812  -1.115  -1.113  -0.986  -0.943  -0.929  -0.813
35    P         1.445   1.452   1.510   1.405   1.413   1.511   1.567   1.577   1.664
36    V         1.445   1.452   1.510   1.312   1.321   1.420   1.567   1.577   1.664
37    L        -0.559  -0.559  -0.719  -0.458  -0.460  -0.673  -0.525  -0.525  -0.706
38    L        -0.903  -0.919  -0.816  -0.920  -0.933  -0.779  -0.943  -0.951  -0.817
39    T        -0.559  -0.625  -0.715  -0.369  -0.454  -0.596  -0.525  -0.605  -0.701
40    I        -0.773  -0.738  -0.766  -0.869  -0.841  -0.882  -0.804  -0.772  -0.766
41    N        -1.118  -1.141  -1.146  -1.002  -1.029  -1.068  -0.943  -0.966  -0.942
42    N        -0.773  -0.797  -0.787  -0.662  -0.690  -0.652  -0.804  -0.830  -0.788
43    N         0.062   0.100  -0.162   0.360   0.417  -0.011   0.033   0.086  -0.293
44    V         0.062   0.049  -0.157   0.034   0.017  -0.280   0.033   0.015  -0.286
45    V        -0.300  -0.269  -0.286  -0.360  -0.331  -0.303  -0.525  -0.490  -0.481
46    H        -1.247  -1.269  -1.195  -0.941  -0.982  -0.878  -1.083  -1.096  -0.988
47    I        -0.773  -0.759  -0.772  -1.022  -1.002  -1.011  -0.804  -0.781  -0.768
48    A        -0.773  -0.749  -0.778  -0.613  -0.615  -0.603  -0.804  -0.786  -0.779
49    Q        -1.118  -1.159  -1.170  -0.796  -0.861  -0.916  -0.943  -0.984  -0.964
50    V         0.406   0.431   0.402   0.230   0.278   0.183   0.172   0.215   0.125
51    K        -1.247  -1.242  -1.185  -1.158  -1.130  -1.068  -1.083  -1.073  -0.979
52    G         1.445   1.452   1.510   1.414   1.422   1.519   1.567   1.577   1.664
53    T        -1.591  -1.599  -1.499  -1.401  -1.410  -1.256  -1.222  -1.224  -1.097
54    S         1.445   1.452   1.510   1.414   1.436   1.519   1.567   1.577   1.664
55    M         1.445   1.452   1.510   1.655   1.664   1.752   1.567   1.577   1.664
56    Q        -0.773  -0.781  -0.783  -0.701  -0.713  -0.715  -0.804  -0.808  -0.783
57    P         1.445   1.452   1.510   1.405   1.413   1.511   1.567   1.577   1.664
58    T         0.259   0.251   0.335   0.124   0.115   0.249  -0.107  -0.124  -0.005
59    L         0.259   0.263   0.345   0.004   0.002   0.145  -0.107  -0.101   0.014
60    N         1.445   1.452   1.510   1.505   1.516   1.607   1.567   1.577   1.664
61    P         1.445   1.452   1.510   1.405   1.413   1.511   1.567   1.577   1.664
62    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
63    Q        -0.865  -0.879  -0.810  -0.841  -0.840  -0.722  -0.901  -0.910  -0.809
64    T         0.339   0.336   0.360   0.185   0.182   0.184   0.048   0.039   0.044
65    E        -0.865  -0.826  -0.798  -0.842  -0.790  -0.750  -0.901  -0.859  -0.799
66    T        -1.118  -1.159  -1.170  -0.989  -1.053  -1.090  -0.943  -0.984  -0.964
67    L        -0.773  -0.757  -0.766  -0.790  -0.778  -0.779  -0.804  -0.779  -0.762
68    A        -1.591  -1.546  -1.495  -1.298  -1.222  -1.180  -1.222  -1.181  -1.094
69    T        -1.247  -1.195  -1.179  -1.262  -1.176  -1.153  -1.083  -1.034  -0.975
70    D         1.445   1.452   1.510   1.530   1.546   1.631   1.567   1.577   1.664
71    W        -0.773  -0.792  -0.780  -0.724  -0.758  -0.769  -0.804  -0.823  -0.780
72    V         0.733   0.777   0.538   0.681   0.737   0.426   0.730   0.799   0.378
73    L         1.445   1.452   1.510   1.380   1.386   1.486   1.567   1.577   1.664
74    L         0.406   0.447   0.436   0.165   0.234   0.194   0.172   0.245   0.190
75    W         0.259   0.268   0.345   0.274   0.289   0.403  -0.107  -0.092   0.014
76    K         1.445   1.452   1.510   1.435   1.449   1.539   1.567   1.577   1.664
77    F        -0.215  -0.215  -0.253  -0.095  -0.112  -0.138  -0.386  -0.396  -0.433
78    G        -0.215  -0.232  -0.262  -0.291  -0.314  -0.388  -0.386  -0.399  -0.441
79    V        -0.463  -0.453  -0.352  -0.841  -0.821  -0.659  -0.711  -0.696  -0.560
80    K         0.662   0.661   0.494   0.700   0.700   0.439   0.618   0.614   0.298
81    N        -0.865  -0.848  -0.810  -0.860  -0.830  -0.776  -0.901  -0.880  -0.809
82    P        -0.062  -0.041  -0.221  -0.062  -0.037  -0.312  -0.141  -0.112  -0.377
83    S        -0.903  -0.892  -0.813  -1.056  -1.033  -0.894  -0.943  -0.929  -0.815
84    N        -1.247  -1.281  -1.194  -1.205  -1.234  -1.102  -1.083  -1.106  -0.986
85    L        -0.559  -0.580  -0.718  -0.462  -0.496  -0.677  -0.525  -0.551  -0.704
86    S        -0.773  -0.725  -0.760  -0.809  -0.729  -0.762  -0.804  -0.745  -0.758
87    R         0.062   0.058  -0.179   0.212   0.196  -0.171   0.033   0.026  -0.317
88    D        -0.215  -0.167  -0.260  -0.299  -0.229  -0.357  -0.386  -0.319  -0.438
89    D         1.445   1.452   1.510   1.530   1.546   1.631   1.567   1.577   1.664
90    I         0.406   0.444   0.407   0.152   0.216   0.140   0.172   0.239   0.135
91    I         0.259   0.254   0.335  -0.087  -0.106   0.017  -0.107  -0.119  -0.005
92    L        -0.215  -0.241  -0.248  -0.278  -0.317  -0.319  -0.386  -0.407  -0.419
93    F         0.406   0.353   0.365   0.351   0.235   0.221   0.172   0.072   0.054
94    K         0.406   0.449   0.398   0.336   0.408   0.288   0.172   0.249   0.117
95    A         0.062   0.054  -0.174   0.153   0.151  -0.183   0.033   0.021  -0.310
96    P         1.445   1.452   1.510   1.405   1.413   1.511   1.567   1.577   1.664
97    T        -1.247  -1.195  -1.175  -0.991  -0.908  -0.895  -1.083  -1.036  -0.972
98    N         0.259   0.264   0.337   0.097   0.107   0.217  -0.107  -0.099  -0.000
99    P         0.733   0.742   0.538   0.753   0.765   0.469   0.730   0.744   0.378
100   R        -1.118  -1.095  -1.156  -0.889  -0.841  -0.997  -0.943  -0.919  -0.952
101   K         0.733   0.663   0.524   0.805   0.722   0.520   0.730   0.618   0.353
102   V        -0.300  -0.320  -0.294  -0.610  -0.636  -0.560  -0.525  -0.543  -0.491
103   Y         0.259   0.279   0.345   0.074   0.098   0.214  -0.107  -0.071   0.014
104   C        -0.215  -0.241  -0.248  -0.082  -0.114  -0.170  -0.386  -0.407  -0.419
105   K         1.445   1.452   1.510   1.435   1.449   1.539   1.567   1.577   1.664
106   R         1.445   1.452   1.510   1.509   1.515   1.611   1.567   1.577   1.664
107   V         0.733   0.691   0.523   0.654   0.598   0.345   0.730   0.663   0.351
108   K        -0.429  -0.430  -0.337  -0.783  -0.790  -0.644  -0.664  -0.665  -0.541
109   G         1.445   1.452   1.510   1.414   1.422   1.519   1.567   1.577   1.664
110   L        -0.429  -0.396  -0.336  -0.809  -0.755  -0.658  -0.664  -0.618  -0.540
111   P        -0.559  -0.621  -0.729  -0.261  -0.355  -0.569  -0.525  -0.601  -0.717
112   F        -0.429  -0.421  -0.347  -0.531  -0.518  -0.388  -0.664  -0.653  -0.554
113   D         1.445   1.452   1.510   1.530   1.546   1.631   1.567   1.577   1.664
114   T        -1.247  -1.226  -1.183  -1.201  -1.160  -1.091  -1.083  -1.061  -0.978
115   I         0.062   0.033  -0.177   0.158   0.127  -0.208   0.033  -0.010  -0.314
116   D        -1.591  -1.561  -1.496  -1.534  -1.498  -1.397  -1.222  -1.193  -1.095
117   T         0.733   0.766   0.554   0.815   0.867   0.543   0.730   0.782   0.407
118   K         0.259   0.284   0.354   0.041   0.091   0.196  -0.107  -0.060   0.033
119   F        -1.118  -1.141  -1.146  -1.007  -1.038  -1.047  -0.943  -0.966  -0.942
120   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
121   P         1.445   1.452   1.510   1.405   1.413   1.511   1.567   1.577   1.664
122   Y         0.733   0.663   0.524   0.994   0.900   0.662   0.730   0.618   0.353
123   P         0.733   0.663   0.524   0.745   0.648   0.430   0.730   0.618   0.353
124   K        -0.559  -0.617  -0.722  -0.445  -0.535  -0.696  -0.525  -0.596  -0.710
125   P        -0.903  -0.910  -0.816  -1.071  -1.071  -0.936  -0.943  -0.944  -0.818
126   Q        -0.773  -0.778  -0.772  -0.687  -0.696  -0.677  -0.804  -0.801  -0.771
127   V        -0.215  -0.184  -0.261  -0.272  -0.235  -0.294  -0.386  -0.349  -0.442
128   N        -1.936  -1.919  -1.789  -1.766  -1.747  -1.557  -1.362  -1.345  -1.197
129   L        -0.300  -0.267  -0.283  -0.601  -0.560  -0.565  -0.525  -0.485  -0.476
130   P         1.445   1.452   1.510   1.405   1.413   1.511   1.567   1.577   1.664
131   R         0.062  -0.041  -0.198   0.257   0.112  -0.120   0.033  -0.112  -0.344
132   G         0.406   0.389   0.373   0.324   0.288   0.222   0.172   0.138   0.069
133   H         1.445   1.452   1.510   1.663   1.675   1.760   1.567   1.577   1.664
134   I        -0.773  -0.753  -0.766  -0.967  -0.967  -0.944  -0.804  -0.789  -0.766
135   W         1.445   1.452   1.510   1.631   1.641   1.729   1.567   1.577   1.664
136   V         1.445   1.452   1.510   1.312   1.321   1.420   1.567   1.577   1.664
137   E         1.445   1.452   1.510   1.615   1.636   1.714   1.567   1.577   1.664
138   G         1.445   1.452   1.510   1.414   1.422   1.519   1.567   1.577   1.664
139   D         1.445   1.452   1.510   1.530   1.546   1.631   1.567   1.577   1.664
140   N         0.733   0.663   0.524   0.875   0.787   0.584   0.730   0.618   0.353
141   Y        -1.591  -1.557  -1.497  -1.397  -1.337  -1.255  -1.222  -1.191  -1.096
142   F        -0.062  -0.045  -0.222   0.130   0.151  -0.129  -0.141  -0.117  -0.379
143   H         0.733   0.742   0.538   1.014   1.028   0.718   0.730   0.744   0.378
144   S         1.445   1.452   1.510   1.414   1.436   1.519   1.567   1.577   1.664
145   I        -0.215  -0.216  -0.261  -0.353  -0.333  -0.436  -0.386  -0.374  -0.443
146   D         1.445   1.452   1.510   1.530   1.546   1.631   1.567   1.577   1.664
147   S         1.445   1.452   1.510   1.414   1.436   1.519   1.567   1.577   1.664
148   N         1.445   1.452   1.510   1.505   1.516   1.607   1.567   1.577   1.664
149   T        -0.300  -0.271  -0.277  -0.442  -0.403  -0.388  -0.525  -0.493  -0.470
150   F         1.445   1.452   1.510   1.546   1.550   1.647   1.567   1.577   1.664
151   G         1.445   1.452   1.510   1.414   1.422   1.519   1.567   1.577   1.664
152   P         1.445   1.452   1.510   1.405   1.413   1.511   1.567   1.577   1.664
153   I         0.259   0.253   0.337  -0.087  -0.108   0.027  -0.107  -0.121  -0.001
154   S         1.445   1.452   1.510   1.414   1.436   1.519   1.567   1.577   1.664
155   S        -0.903  -0.911  -0.816  -0.963  -0.972  -0.845  -0.943  -0.944  -0.816
156   G         0.733   0.777   0.538   0.783   0.838   0.520   0.730   0.799   0.378
157   L         1.445   1.452   1.510   1.380   1.386   1.486   1.567   1.577   1.664
158   V        -0.559  -0.618  -0.737  -0.523  -0.612  -0.751  -0.525  -0.597  -0.726
159   I        -1.118  -1.159  -1.170  -1.056  -1.114  -1.163  -0.943  -0.984  -0.964
160   G         0.733   0.663   0.524   0.783   0.691   0.498   0.730   0.618   0.353
161   K         1.445   1.452   1.510   1.435   1.449   1.539   1.567   1.577   1.664
162   A        -0.300  -0.320  -0.294  -0.396  -0.423  -0.336  -0.525  -0.543  -0.491
163   I        -0.300  -0.319  -0.287  -0.595  -0.611  -0.551  -0.525  -0.540  -0.482
164   T        -1.247  -1.250  -1.181  -1.157  -1.149  -1.035  -1.083  -1.080  -0.976
165   I         0.406   0.389   0.377   0.230   0.198   0.127   0.172   0.139   0.077
166   V        -0.429  -0.429  -0.347  -0.772  -0.778  -0.658  -0.664  -0.664  -0.554
167   W         0.733   0.663   0.524   0.988   0.894   0.676   0.730   0.618   0.353
168   P         1.445   1.452   1.510   1.405   1.413   1.511   1.567   1.577   1.664
169   P        -0.215  -0.223  -0.273  -0.307  -0.338  -0.393  -0.386  -0.413  -0.460
170   S         0.733   0.691   0.523   0.765   0.722   0.461   0.730   0.663   0.351
171   R         1.445   1.452   1.510   1.509   1.515   1.611   1.567   1.577   1.664
172   W        -0.300  -0.337  -0.295  -0.243  -0.294  -0.212  -0.525  -0.561  -0.492
173   G         0.062   0.104  -0.161   0.114   0.173  -0.230   0.033   0.091  -0.291
174   T        -0.773  -0.778  -0.772  -0.699  -0.695  -0.712  -0.804  -0.801  -0.771
175   D        -0.215  -0.231  -0.268  -0.200  -0.242  -0.286  -0.386  -0.422  -0.453
176   L        -0.559  -0.617  -0.722  -0.540  -0.633  -0.805  -0.525  -0.596  -0.710
177   K        -1.266  -1.288  -1.197  -1.175  -1.196  -1.079  -1.091  -1.108  -0.988
178   L        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
179   S        -0.361  -0.385  -0.321  -0.512  -0.544  -0.446  -0.569  -0.603  -0.518
180   T        -0.463  -0.453  -0.351  -0.604  -0.591  -0.429  -0.711  -0.696  -0.560
181   G         1.445   1.452   1.510   1.425   1.431   1.529   1.567   1.577   1.664
182   R         1.445   1.452   1.510   1.504   1.515   1.606   1.567   1.577   1.664
183   D        -0.388  -0.355  -0.320  -0.437  -0.385  -0.340  -0.620  -0.580  -0.521
184   C        -0.865  -0.855  -0.802  -0.680  -0.671  -0.598  -0.901  -0.888  -0.802
185   I         0.241   0.256   0.339   0.011   0.041   0.172  -0.141  -0.116   0.003
186   S        -0.062   0.009  -0.180  -0.072   0.037  -0.285  -0.141  -0.040  -0.319
187   K        -1.668  -1.650  -1.528  -1.621  -1.575  -1.417  -1.280  -1.263  -1.118
188   R        -1.247  -1.195  -1.174  -1.134  -1.064  -1.020  -1.083  -1.035  -0.971
189   A        -1.591  -1.590  -1.508  -1.536  -1.567  -1.410  -1.222  -1.217  -1.104
190   I        -1.591  -1.599  -1.518  -1.413  -1.431  -1.312  -1.222  -1.225  -1.111
191   -        -0.206  -0.229  -0.276  -0.209  -0.250  -0.289  -0.347  -0.380  -0.455
192   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
193   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
194   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
195   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
196   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
197   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
198   -        -0.963  -0.954  -0.840  -1.135  -1.124  -0.946  -0.996  -0.983  -0.840
199   L        -1.266  -1.246  -1.184  -1.186  -1.179  -1.092  -1.091  -1.069  -0.977
200   E        -1.266  -1.212  -1.182  -1.251  -1.175  -1.127  -1.091  -1.036  -0.975
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P46972
Output conservation file - output/P46972
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
