1     M         1.567   1.568   1.592   1.907   1.908   1.947   1.652   1.654   1.683
2     N         1.567   1.568   1.592   1.556   1.559   1.596   1.652   1.654   1.683
3     S         0.054   0.064   0.020  -0.212  -0.195  -0.268  -0.086  -0.070  -0.139
4     G         1.567   1.568   1.592   1.907   1.909   1.947   1.652   1.654   1.683
5     G         0.054   0.064   0.020   0.131   0.144   0.095  -0.086  -0.070  -0.139
6     S        -1.045  -1.043  -1.022  -0.802  -0.796  -0.763  -0.955  -0.953  -0.914
7     D         1.567   1.568   1.592   1.724   1.728   1.764   1.652   1.654   1.683
8     S         0.054   0.069   0.022  -0.025   0.001  -0.081  -0.086  -0.062  -0.136
9     F         1.567   1.568   1.592   1.840   1.844   1.880   1.652   1.654   1.683
10    D         0.054   0.064   0.020  -0.125  -0.110  -0.167  -0.086  -0.070  -0.139
11    Y         1.567   1.568   1.592   1.724   1.726   1.764   1.652   1.654   1.683
12    L         1.567   1.568   1.592   1.349   1.352   1.389   1.652   1.654   1.683
13    L         0.054   0.069   0.022  -0.207  -0.183  -0.257  -0.086  -0.062  -0.136
14    Q         0.054   0.069   0.022  -0.072  -0.047  -0.145  -0.086  -0.062  -0.136
15    L         1.567   1.568   1.592   1.349   1.352   1.389   1.652   1.654   1.683
16    T        -1.045  -1.043  -1.022  -0.660  -0.656  -0.621  -0.955  -0.953  -0.914
17    K         1.567   1.568   1.592   1.391   1.394   1.431   1.652   1.654   1.683
18    A        -1.045  -1.043  -1.022  -0.855  -0.852  -0.816  -0.955  -0.953  -0.914
19    L         1.567   1.568   1.592   1.349   1.352   1.389   1.652   1.654   1.683
20    S         1.567   1.568   1.592   1.545   1.548   1.585   1.652   1.654   1.683
21    A        -1.045  -1.043  -1.022  -0.820  -0.825  -0.780  -0.955  -0.953  -0.914
22    E        -1.045  -1.043  -1.022  -0.982  -0.986  -0.942  -0.955  -0.953  -0.914
23    C         0.054   0.033   0.010   0.470   0.434   0.391  -0.086  -0.120  -0.156
24    R         0.054   0.033   0.010   0.334   0.305   0.279  -0.086  -0.120  -0.156
25    A         0.054   0.069   0.022   0.269   0.295   0.214  -0.086  -0.062  -0.136
26    N        -1.045  -1.043  -1.022  -1.006  -1.000  -0.967  -0.955  -0.953  -0.914
27    R         1.567   1.568   1.592   1.744   1.748   1.784   1.652   1.654   1.683
28    Q         0.054   0.069   0.022  -0.053  -0.028  -0.123  -0.086  -0.062  -0.136
29    E         0.054   0.033   0.010  -0.183  -0.218  -0.250  -0.086  -0.120  -0.156
30    T         1.567   1.568   1.592   1.724   1.726   1.764   1.652   1.654   1.683
31    D         0.054   0.033   0.010   0.045   0.002  -0.058  -0.086  -0.120  -0.156
32    R         0.054   0.064   0.020  -0.087  -0.072  -0.129  -0.086  -0.070  -0.139
33    I         0.054   0.033   0.010   0.131   0.100   0.071  -0.086  -0.120  -0.156
34    E         1.567   1.568   1.592   1.360   1.364   1.400   1.652   1.654   1.683
35    L         0.054   0.033   0.010  -0.193  -0.229  -0.259  -0.086  -0.120  -0.156
36    L         0.054   0.033   0.010  -0.222  -0.258  -0.294  -0.086  -0.120  -0.156
37    L         1.567   1.568   1.592   1.349   1.352   1.389   1.652   1.654   1.683
38    K         1.567   1.568   1.592   1.391   1.394   1.431   1.652   1.654   1.683
39    R         1.567   1.568   1.592   1.744   1.748   1.784   1.652   1.654   1.683
40    L         1.567   1.568   1.592   1.349   1.352   1.389   1.652   1.654   1.683
41    A         0.054   0.069   0.022   0.189   0.218   0.124  -0.086  -0.062  -0.136
42    K         1.567   1.568   1.592   1.391   1.394   1.431   1.652   1.654   1.683
43    Q         1.567   1.568   1.592   1.605   1.604   1.645   1.652   1.654   1.683
44    S         0.054   0.033   0.010  -0.025  -0.061  -0.103  -0.086  -0.120  -0.156
45    G        -1.045  -1.043  -1.022  -0.716  -0.724  -0.677  -0.955  -0.953  -0.914
46    I         0.054   0.069   0.022   0.131   0.154   0.089  -0.086  -0.062  -0.136
47    S         1.567   1.568   1.592   1.545   1.548   1.585   1.652   1.654   1.683
48    Y         1.567   1.568   1.592   1.724   1.726   1.764   1.652   1.654   1.683
49    D        -1.045  -1.043  -1.022  -1.120  -1.115  -1.081  -0.955  -0.953  -0.914
50    N         0.054   0.064   0.020  -0.198  -0.181  -0.251  -0.086  -0.070  -0.139
51    L        -1.045  -1.043  -1.022  -0.957  -0.954  -0.918  -0.955  -0.953  -0.914
52    S         1.567   1.568   1.592   1.545   1.548   1.585   1.652   1.654   1.683
53    K         0.054   0.069   0.022  -0.154  -0.132  -0.203  -0.086  -0.062  -0.136
54    N        -1.045  -1.043  -1.022  -0.994  -0.994  -0.955  -0.955  -0.953  -0.914
55    I        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
56    I        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
57    P         1.567   1.568   1.592   1.898   1.900   1.938   1.652   1.654   1.683
58    D        -1.045  -1.043  -1.022  -1.006  -1.005  -0.967  -0.955  -0.953  -0.914
59    S        -1.045  -1.043  -1.022  -1.140  -1.129  -1.100  -0.955  -0.953  -0.914
60    W         0.054   0.064   0.020  -0.006   0.006  -0.032  -0.086  -0.070  -0.139
61    K         0.054   0.064   0.020   0.058   0.076  -0.019  -0.086  -0.070  -0.139
62    D         0.054   0.064   0.020  -0.134  -0.120  -0.177  -0.086  -0.070  -0.139
63    N        -1.045  -1.043  -1.022  -0.785  -0.777  -0.745  -0.955  -0.953  -0.914
64    A         0.054   0.033   0.010   0.216   0.176   0.131  -0.086  -0.120  -0.156
65    S        -1.045  -1.043  -1.022  -0.861  -0.856  -0.822  -0.955  -0.953  -0.914
66    Q        -1.045  -1.043  -1.022  -1.179  -1.177  -1.140  -0.955  -0.953  -0.914
67    K         0.054   0.033   0.010  -0.096  -0.128  -0.154  -0.086  -0.120  -0.156
68    A         0.054   0.064   0.020  -0.101  -0.087  -0.138  -0.086  -0.070  -0.139
69    S         0.054   0.033   0.010  -0.110  -0.151  -0.204  -0.086  -0.120  -0.156
70    P        -1.045  -1.043  -1.022  -0.988  -0.988  -0.948  -0.955  -0.953  -0.914
71    P         0.054   0.033   0.010   0.221   0.175   0.114  -0.086  -0.120  -0.156
72    T         1.567   1.568   1.592   1.724   1.726   1.764   1.652   1.654   1.683
73    E        -1.045  -1.043  -1.022  -1.239  -1.243  -1.200  -0.955  -0.953  -0.914
74    A        -1.045  -1.043  -1.022  -0.982  -0.990  -0.942  -0.955  -0.953  -0.914
75    Q         0.054   0.064   0.020  -0.183  -0.169  -0.234  -0.086  -0.070  -0.139
76    K        -1.045  -1.043  -1.022  -1.321  -1.326  -1.281  -0.955  -0.953  -0.914
77    L         1.567   1.568   1.592   1.349   1.352   1.389   1.652   1.654   1.683
78    I         0.054   0.033   0.010   0.131   0.100   0.071  -0.086  -0.120  -0.156
79    S        -1.045  -1.043  -1.022  -1.127  -1.132  -1.087  -0.955  -0.953  -0.914
80    E         1.567   1.568   1.592   1.360   1.364   1.400   1.652   1.654   1.683
81    N         1.567   1.568   1.592   1.575   1.579   1.615   1.652   1.654   1.683
82    F         0.054   0.064   0.020   0.260   0.274   0.212  -0.086  -0.070  -0.139
83    K        -1.045  -1.043  -1.022  -1.069  -1.063  -1.030  -0.955  -0.953  -0.914
84    L         1.567   1.568   1.592   1.349   1.352   1.389   1.652   1.654   1.683
85    I         0.054   0.064   0.020   0.317   0.333   0.247  -0.086  -0.070  -0.139
86    Y         1.567   1.568   1.592   1.724   1.726   1.764   1.652   1.654   1.683
87    E         0.054   0.033   0.010  -0.183  -0.218  -0.250  -0.086  -0.120  -0.156
88    I         1.567   1.568   1.592   1.595   1.599   1.635   1.652   1.654   1.683
89    E         1.567   1.568   1.592   1.360   1.364   1.400   1.652   1.654   1.683
90    K        -1.045  -1.043  -1.022  -0.951  -0.942  -0.912  -0.955  -0.953  -0.914
91    Q         1.567   1.568   1.592   1.605   1.604   1.645   1.652   1.654   1.683
92    E         1.567   1.568   1.592   1.360   1.364   1.400   1.652   1.654   1.683
93    Y         1.567   1.568   1.592   1.724   1.726   1.764   1.652   1.654   1.683
94    F         0.054   0.064   0.020   0.145   0.160   0.102  -0.086  -0.070  -0.139
95    N         0.054   0.033   0.010   0.167   0.139   0.117  -0.086  -0.120  -0.156
96    T        -1.045  -1.043  -1.022  -1.120  -1.118  -1.081  -0.955  -0.953  -0.914
97    K         0.054   0.069   0.022  -0.087  -0.064  -0.126  -0.086  -0.062  -0.136
98    A         0.054   0.064   0.020   0.122   0.138   0.076  -0.086  -0.070  -0.139
99    V        -1.045  -1.043  -1.022  -0.831  -0.829  -0.792  -0.955  -0.953  -0.914
100   A         0.054   0.033   0.010   0.104   0.060  -0.002  -0.086  -0.120  -0.156
101   L         1.567   1.568   1.592   1.349   1.352   1.389   1.652   1.654   1.683
102   I         0.054   0.069   0.022   0.131   0.154   0.089  -0.086  -0.062  -0.136
103   N         0.054   0.033   0.010   0.017  -0.020  -0.059  -0.086  -0.120  -0.156
104   N        -1.045  -1.043  -1.022  -1.082  -1.081  -1.043  -0.955  -0.953  -0.914
105   I         1.567   1.568   1.592   1.595   1.599   1.635   1.652   1.654   1.683
106   N         0.054   0.033   0.010   0.072   0.040   0.006  -0.086  -0.120  -0.156
107   E         1.567   1.568   1.592   1.360   1.364   1.400   1.652   1.654   1.683
108   H         0.054   0.033   0.010   0.470   0.435   0.402  -0.086  -0.120  -0.156
109   F        -1.045  -1.043  -1.022  -1.038  -1.034  -0.998  -0.955  -0.953  -0.914
110   S        -1.045  -1.043  -1.022  -0.939  -0.931  -0.900  -0.955  -0.953  -0.914
111   Y         0.054   0.064   0.020   0.045   0.060  -0.004  -0.086  -0.070  -0.139
112   I         0.054   0.033   0.010   0.176   0.147   0.124  -0.086  -0.120  -0.156
113   K         0.054   0.064   0.020   0.077   0.097  -0.001  -0.086  -0.070  -0.139
114   N         1.567   1.568   1.592   1.575   1.579   1.615   1.652   1.654   1.683
115   F         1.567   1.568   1.592   1.840   1.844   1.880   1.652   1.654   1.683
116   I         1.567   1.568   1.592   1.595   1.599   1.635   1.652   1.654   1.683
117   D        -1.045  -1.043  -1.022  -0.831  -0.825  -0.792  -0.955  -0.953  -0.914
118   E         1.567   1.568   1.592   1.360   1.364   1.400   1.652   1.654   1.683
119   Q         0.054   0.033   0.010   0.100   0.062   0.032  -0.086  -0.120  -0.156
120   N         0.054   0.064   0.020  -0.168  -0.153  -0.222  -0.086  -0.070  -0.139
121   A        -1.045  -1.043  -1.022  -0.988  -0.982  -0.948  -0.955  -0.953  -0.914
122   I        -1.045  -1.043  -1.022  -0.951  -0.953  -0.912  -0.955  -0.953  -0.914
123   R         0.054   0.033   0.010   0.256   0.223   0.187  -0.086  -0.120  -0.156
124   E        -1.045  -1.043  -1.022  -0.915  -0.905  -0.875  -0.955  -0.953  -0.914
125   R         0.054   0.064   0.020   0.109   0.121   0.059  -0.086  -0.070  -0.139
126   N         0.054   0.033   0.010  -0.082  -0.123  -0.176  -0.086  -0.120  -0.156
127   I        -1.045  -1.043  -1.022  -1.050  -1.049  -1.011  -0.955  -0.953  -0.914
128   A        -1.045  -1.043  -1.022  -1.057  -1.055  -1.017  -0.955  -0.953  -0.914
129   T        -1.045  -1.043  -1.022  -1.107  -1.113  -1.068  -0.955  -0.953  -0.914
130   F         0.054   0.069   0.022   0.312   0.338   0.256  -0.086  -0.062  -0.136
131   S        -1.045  -1.043  -1.022  -0.879  -0.882  -0.839  -0.955  -0.953  -0.914
132   S        -1.045  -1.043  -1.022  -0.791  -0.791  -0.751  -0.955  -0.953  -0.914
133   E         0.054   0.033   0.010  -0.287  -0.325  -0.373  -0.086  -0.120  -0.156
134   K        -1.045  -1.043  -1.022  -1.219  -1.215  -1.179  -0.955  -0.953  -0.914
135   L        -1.045  -1.043  -1.022  -0.903  -0.898  -0.863  -0.955  -0.953  -0.914
136   D         0.054   0.064   0.020   0.207   0.223   0.152  -0.086  -0.070  -0.139
137   E        -1.045  -1.043  -1.022  -0.927  -0.921  -0.887  -0.955  -0.953  -0.914
138   R         0.054   0.033   0.010   0.304   0.273   0.244  -0.086  -0.120  -0.156
139   N        -1.045  -1.043  -1.022  -1.000  -0.987  -0.961  -0.955  -0.953  -0.914
140   K        -1.045  -1.043  -1.022  -1.219  -1.215  -1.179  -0.955  -0.953  -0.914
141   S        -1.045  -1.043  -1.022  -1.133  -1.122  -1.094  -0.955  -0.953  -0.914
142   L         1.567   1.568   1.592   1.349   1.352   1.389   1.652   1.654   1.683
143   Q         0.054   0.064   0.020  -0.154  -0.140  -0.206  -0.086  -0.070  -0.139
144   Q        -1.045  -1.043  -1.022  -0.969  -0.967  -0.930  -0.955  -0.953  -0.914
145   N         0.054   0.064   0.020  -0.025  -0.008  -0.084  -0.086  -0.070  -0.139
146   Y        -1.045  -1.043  -1.022  -1.025  -1.033  -0.986  -0.955  -0.953  -0.914
147   E         0.054   0.033   0.010  -0.287  -0.325  -0.373  -0.086  -0.120  -0.156
148   S        -1.045  -1.043  -1.022  -1.225  -1.221  -1.186  -0.955  -0.953  -0.914
149   L         1.567   1.568   1.592   1.349   1.352   1.389   1.652   1.654   1.683
150   K         0.054   0.033   0.010  -0.168  -0.203  -0.240  -0.086  -0.120  -0.156
151   T        -1.045  -1.043  -1.022  -0.768  -0.767  -0.728  -0.955  -0.953  -0.914
152   E        -1.045  -1.043  -1.022  -0.897  -0.887  -0.857  -0.955  -0.953  -0.914
153   N        -1.045  -1.043  -1.022  -0.988  -0.973  -0.948  -0.955  -0.953  -0.914
154   E        -1.045  -1.043  -1.022  -1.186  -1.183  -1.146  -0.955  -0.953  -0.914
155   E        -1.045  -1.043  -1.022  -0.951  -0.953  -0.912  -0.955  -0.953  -0.914
156   T        -1.045  -1.043  -1.022  -0.873  -0.867  -0.833  -0.955  -0.953  -0.914
157   K        -1.045  -1.043  -1.022  -1.225  -1.229  -1.186  -0.955  -0.953  -0.914
158   K         0.054   0.069   0.022  -0.096  -0.074  -0.137  -0.086  -0.062  -0.136
159   K        -1.045  -1.043  -1.022  -1.179  -1.177  -1.140  -0.955  -0.953  -0.914
160   L         0.054   0.069   0.022  -0.134  -0.113  -0.174  -0.086  -0.062  -0.136
161   H        -1.045  -1.043  -1.022  -1.101  -1.105  -1.062  -0.955  -0.953  -0.914
162   S        -1.045  -1.043  -1.022  -0.939  -0.940  -0.900  -0.955  -0.953  -0.914
163   I        -1.045  -1.043  -1.022  -1.057  -1.065  -1.017  -0.955  -0.953  -0.914
164   I        -1.045  -1.043  -1.022  -0.722  -0.717  -0.682  -0.955  -0.953  -0.914
165   K        -1.045  -1.043  -1.022  -1.114  -1.099  -1.075  -0.955  -0.953  -0.914
166   Q        -1.045  -1.043  -1.022  -1.321  -1.324  -1.281  -0.955  -0.953  -0.914
167   F         0.054   0.033   0.010   0.149   0.105   0.042  -0.086  -0.120  -0.156
168   E        -1.045  -1.043  -1.022  -1.239  -1.228  -1.200  -0.955  -0.953  -0.914
169   K         0.054   0.033   0.010  -0.096  -0.127  -0.154  -0.086  -0.120  -0.156
170   L         1.567   1.568   1.592   1.349   1.352   1.389   1.652   1.654   1.683
171   L        -1.045  -1.043  -1.022  -0.957  -0.949  -0.918  -0.955  -0.953  -0.914
172   K        -1.045  -1.043  -1.022  -1.057  -1.056  -1.017  -0.955  -0.953  -0.914
173   E         0.054   0.033   0.010  -0.115  -0.144  -0.170  -0.086  -0.120  -0.156
174   V         0.054   0.069   0.022   0.229   0.256   0.166  -0.086  -0.062  -0.136
175   D         0.054   0.033   0.010   0.286   0.256   0.227  -0.086  -0.120  -0.156
176   W         1.567   1.568   1.592   1.964   1.967   2.004   1.652   1.654   1.683
177   D         0.054   0.033   0.010   0.299   0.269   0.243  -0.086  -0.120  -0.156
178   R        -1.045  -1.043  -1.022  -0.994  -1.001  -0.955  -0.955  -0.953  -0.914
179   I        -1.045  -1.043  -1.022  -1.057  -1.046  -1.017  -0.955  -0.953  -0.914
180   S         0.054   0.064   0.020   0.286   0.304   0.214  -0.086  -0.070  -0.139
181   K        -1.045  -1.043  -1.022  -1.219  -1.214  -1.179  -0.955  -0.953  -0.914
182   D         0.054   0.069   0.022   0.140   0.168   0.079  -0.086  -0.062  -0.136
183   S        -1.045  -1.043  -1.022  -1.246  -1.239  -1.206  -0.955  -0.953  -0.914
184   R        -1.045  -1.043  -1.022  -0.909  -0.919  -0.869  -0.955  -0.953  -0.914
185   D         0.054   0.033   0.010   0.140   0.103   0.055  -0.086  -0.120  -0.156
186   Y         0.054   0.069   0.022   0.149   0.176   0.089  -0.086  -0.062  -0.136
187   S        -1.045  -1.043  -1.022  -1.232  -1.236  -1.193  -0.955  -0.953  -0.914
188   R         0.054   0.069   0.022   0.172   0.198   0.111  -0.086  -0.062  -0.136
189   F        -1.045  -1.043  -1.022  -0.903  -0.914  -0.863  -0.955  -0.953  -0.914
190   K         1.567   1.568   1.592   1.391   1.394   1.431   1.652   1.654   1.683
191   K        -1.045  -1.043  -1.022  -1.342  -1.333  -1.302  -0.955  -0.953  -0.914
192   Q        -1.045  -1.043  -1.022  -1.025  -1.018  -0.986  -0.955  -0.953  -0.914
193   L         0.054   0.064   0.020  -0.082  -0.062  -0.158  -0.086  -0.070  -0.139
194   E        -1.045  -1.043  -1.022  -1.114  -1.099  -1.075  -0.955  -0.953  -0.914
195   Y        -1.045  -1.043  -1.022  -0.688  -0.690  -0.648  -0.955  -0.953  -0.914
196   L        -1.045  -1.043  -1.022  -1.038  -1.026  -0.998  -0.955  -0.953  -0.914
197   Q        -1.045  -1.043  -1.022  -1.321  -1.324  -1.281  -0.955  -0.953  -0.914
198   D         0.054   0.069   0.022   0.045   0.076  -0.031  -0.086  -0.062  -0.136
199   T         0.054   0.033   0.010   0.058   0.015  -0.041  -0.086  -0.120  -0.156
200   Y         1.567   1.568   1.592   1.724   1.726   1.764   1.652   1.654   1.683
201   Q         0.054   0.069   0.022  -0.053  -0.028  -0.123  -0.086  -0.062  -0.136
202   V        -1.045  -1.043  -1.022  -0.976  -0.970  -0.936  -0.955  -0.953  -0.914
203   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
204   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
205   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
206   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
207   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
208   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
209   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
210   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
211   L         0.054   0.069   0.022  -0.134  -0.112  -0.174  -0.086  -0.062  -0.136
212   K         0.054   0.069   0.022  -0.277  -0.249  -0.344  -0.086  -0.062  -0.136
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P46671
Output conservation file - output/P46671
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
