1     M         1.236   1.271   1.321   1.645   1.696   1.762   1.321   1.368   1.443
2     A        -0.440  -0.592  -0.634  -0.143  -0.350  -0.401  -0.471  -0.674  -0.716
3     P         1.236   1.271   1.321   1.566   1.622   1.687   1.321   1.368   1.443
4     K        -0.064   0.020   0.067  -0.851  -0.739  -0.609  -0.400  -0.254  -0.199
5     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
6     -         1.236   1.271   1.321   1.323   1.378   1.457   1.321   1.368   1.443
7     -         0.150   0.099   0.084  -0.422  -0.470  -0.419  -0.023  -0.116  -0.167
8     -         0.150   0.099   0.084   0.142   0.001  -0.158  -0.023  -0.116  -0.167
9     -         0.150   0.099   0.084   0.007  -0.124  -0.280  -0.023  -0.116  -0.167
10    K        -0.440  -0.592  -0.634  -0.950  -1.155  -1.109  -0.471  -0.674  -0.716
11    P        -1.164  -1.207  -1.207  -1.041  -1.082  -1.145  -1.069  -1.108  -1.063
12    S        -1.164  -1.211  -1.194  -0.834  -0.926  -0.885  -1.069  -1.112  -1.049
13    K         1.236   1.271   1.321   0.584   0.630   0.756   1.321   1.368   1.443
14    R        -2.225  -2.135  -2.002  -2.216  -2.048  -1.897  -1.666  -1.563  -1.388
15    Q         0.366   0.180   0.148   0.712   0.458   0.382   0.325   0.023  -0.054
16    N        -0.440  -0.592  -0.634  -0.092  -0.338  -0.434  -0.471  -0.674  -0.716
17    L        -1.333  -1.241  -1.218  -1.512  -1.273  -1.216  -1.268  -1.151  -1.075
18    R        -1.164  -1.207  -1.207  -1.017  -1.114  -1.265  -1.069  -1.108  -1.063
19    R         1.236   1.271   1.321   1.269   1.330   1.405   1.321   1.368   1.443
20    E        -0.718  -0.649  -0.660  -0.639  -0.534  -0.528  -0.870  -0.758  -0.751
21    V         1.236   1.271   1.321   1.475   1.527   1.601   1.321   1.368   1.443
22    A        -2.225  -2.135  -2.002  -2.247  -2.304  -1.927  -1.666  -1.563  -1.388
23    P         0.006   0.078   0.079   0.114   0.236   0.220  -0.272  -0.151  -0.176
24    E         1.236   1.271   1.321   1.220   1.267   1.359   1.321   1.368   1.443
25    V         1.236   1.271   1.321   1.475   1.527   1.601   1.321   1.368   1.443
26    F         0.366   0.444   0.186   0.871   0.976   0.599   0.325   0.444   0.012
27    Q        -1.779  -1.741  -1.656  -1.434  -1.347  -1.268  -1.467  -1.409  -1.269
28    D         1.236   1.271   1.321   1.450   1.509   1.577   1.321   1.368   1.443
29    S        -0.440  -0.592  -0.634  -0.334  -0.677  -0.772  -0.471  -0.674  -0.716
30    Q        -1.333  -1.331  -1.235  -1.304  -1.453  -1.237  -1.268  -1.241  -1.091
31    A         1.236   1.271   1.321   1.330   1.384   1.463   1.321   1.368   1.443
32    R         0.006   0.119   0.084  -0.415  -0.156  -0.358  -0.272  -0.080  -0.168
33    N         1.236   1.271   1.321   1.463   1.508   1.589   1.321   1.368   1.443
34    Q         0.006   0.102   0.095   0.128   0.301   0.237  -0.272  -0.111  -0.149
35    L         0.366   0.478   0.190   0.627   0.785   0.413   0.325   0.494   0.019
36    A         0.366   0.444   0.186   0.602   0.709   0.385   0.325   0.444   0.012
37    N        -1.164  -1.180  -1.204  -0.671  -0.667  -0.733  -1.069  -1.080  -1.060
38    V        -1.333  -1.364  -1.234  -0.974  -0.989  -0.845  -1.268  -1.267  -1.091
39    P         0.366   0.180   0.148   0.781   0.515   0.417   0.325   0.023  -0.054
40    H        -0.440  -0.332  -0.596  -0.830  -0.708  -0.884  -0.471  -0.319  -0.665
41    L        -0.440  -0.525  -0.630  -0.092  -0.214  -0.331  -0.471  -0.593  -0.710
42    T         0.366   0.180   0.148   0.764   0.543   0.472   0.325   0.023  -0.054
43    E        -0.718  -0.786  -0.681  -0.603  -0.679  -0.520  -0.870  -0.926  -0.778
44    K         0.366   0.180   0.148  -0.183  -0.423  -0.401   0.325   0.023  -0.054
45    S         0.366   0.180   0.148   0.564   0.303   0.220   0.325   0.023  -0.054
46    A        -2.225  -2.135  -2.002  -2.136  -1.919  -1.822  -1.666  -1.563  -1.388
47    Q        -1.337  -1.315  -1.241  -1.013  -0.986  -0.907  -1.260  -1.213  -1.096
48    R        -0.718  -0.797  -0.684  -1.425  -1.516  -1.283  -0.870  -0.940  -0.783
49    K        -0.440  -0.552  -0.626  -0.937  -1.060  -1.080  -0.471  -0.625  -0.705
50    P        -0.440  -0.337  -0.586   0.043   0.192  -0.211  -0.471  -0.327  -0.653
51    S        -1.164  -1.216  -1.205  -0.844  -0.940  -0.915  -1.069  -1.117  -1.061
52    K         1.236   1.271   1.321   0.584   0.630   0.756   1.321   1.368   1.443
53    T        -1.164  -1.176  -1.189  -0.775  -0.756  -0.799  -1.069  -1.076  -1.044
54    K        -0.886  -0.833  -0.709  -1.297  -1.299  -1.059  -1.069  -0.985  -0.813
55    V        -0.440  -0.519  -0.617  -0.049  -0.170  -0.341  -0.471  -0.586  -0.694
56    K         0.366   0.180   0.148  -0.230  -0.496  -0.504   0.325   0.023  -0.054
57    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
58    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
59    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
60    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
61    K         1.236   1.271   1.321   0.584   0.630   0.756   1.321   1.368   1.443
62    E        -0.886  -0.831  -0.709  -0.841  -0.739  -0.580  -1.069  -0.982  -0.813
63    Q         0.006  -0.024   0.043   0.175   0.115   0.195  -0.272  -0.335  -0.242
64    S        -1.164  -1.154  -1.199  -0.780  -0.763  -0.804  -1.069  -1.053  -1.054
65    L        -0.440  -0.332  -0.596  -0.995  -0.844  -1.150  -0.471  -0.319  -0.665
66    A        -0.718  -0.786  -0.681  -0.526  -0.578  -0.437  -0.870  -0.926  -0.778
67    R         0.366   0.180   0.148   0.336  -0.020  -0.169   0.325   0.023  -0.054
68    L         0.366   0.180   0.148   0.593   0.354   0.281   0.325   0.023  -0.054
69    Y         1.236   1.271   1.321   1.640   1.692   1.757   1.321   1.368   1.443
70    G         1.236   1.271   1.321   1.537   1.589   1.660   1.321   1.368   1.443
71    A         0.150   0.179   0.086   0.211   0.259   0.080  -0.023   0.021  -0.163
72    K        -0.600  -0.567  -0.657  -1.151  -1.092  -1.129  -0.686  -0.632  -0.745
73    K        -0.600  -0.527  -0.665  -1.114  -1.006  -1.079  -0.686  -0.578  -0.756
74    D        -0.718  -0.574  -0.645  -1.156  -1.018  -0.986  -0.870  -0.672  -0.732
75    K         1.236   1.271   1.321   0.584   0.630   0.756   1.321   1.368   1.443
76    K        -0.440  -0.513  -0.622  -0.513  -0.622  -0.940  -0.471  -0.580  -0.700
77    G        -0.772  -0.649  -0.674  -0.696  -0.508  -0.545  -0.919  -0.743  -0.768
78    K        -1.442  -1.371  -1.250  -1.658  -1.420  -1.369  -1.368  -1.270  -1.105
79    Y         1.236   1.271   1.321   1.645   1.700   1.762   1.321   1.368   1.443
80    S        -0.886  -0.831  -0.709  -0.859  -0.728  -0.585  -1.069  -0.982  -0.813
81    E         1.236   1.271   1.321   1.220   1.267   1.359   1.321   1.368   1.443
82    K         0.006   0.102   0.095  -0.654  -0.526  -0.465  -0.272  -0.111  -0.149
83    D        -0.886  -0.831  -0.709  -0.841  -0.776  -0.600  -1.069  -0.982  -0.813
84    L         1.236   1.271   1.321   1.356   1.422   1.488   1.321   1.368   1.443
85    N        -0.718  -0.806  -0.678  -0.453  -0.557  -0.389  -0.870  -0.952  -0.778
86    I         0.366   0.393   0.178   0.703   0.754   0.402   0.325   0.366  -0.001
87    P         1.236   1.271   1.321   1.571   1.628   1.692   1.321   1.368   1.443
88    T        -1.164  -1.237  -1.201  -0.990  -1.258  -1.206  -1.069  -1.137  -1.057
89    L         1.236   1.271   1.321   1.356   1.422   1.488   1.321   1.368   1.443
90    N         1.236   1.271   1.321   1.463   1.508   1.589   1.321   1.368   1.443
91    R        -0.718  -0.776  -0.678  -1.323  -1.330  -1.129  -0.870  -0.911  -0.776
92    A         1.236   1.271   1.321   1.330   1.384   1.463   1.321   1.368   1.443
93    I         0.366   0.393   0.178   0.726   0.775   0.447   0.325   0.366  -0.001
94    V        -1.164  -1.237  -1.201  -0.686  -0.794  -0.756  -1.069  -1.137  -1.057
95    P         1.236   1.271   1.321   1.571   1.628   1.692   1.321   1.368   1.443
96    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
97    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
98    G         0.366   0.180   0.148   0.735   0.458   0.371   0.325   0.023  -0.054
99    V         0.366   0.180   0.148   0.547   0.182   0.028   0.325   0.023  -0.054
100   K         1.236   1.271   1.321   0.584   0.630   0.756   1.321   1.368   1.443
101   I        -0.718  -0.634  -0.662  -0.585  -0.447  -0.502  -0.870  -0.736  -0.755
102   R        -0.718  -0.797  -0.684  -1.375  -1.423  -1.206  -0.870  -0.940  -0.783
103   R         0.006   0.098   0.076  -0.415  -0.207  -0.386  -0.272  -0.117  -0.183
104   G         1.236   1.271   1.321   1.530   1.578   1.653   1.321   1.368   1.443
105   K         1.236   1.271   1.321   0.584   0.630   0.756   1.321   1.368   1.443
106   K         1.236   1.271   1.321   0.584   0.630   0.756   1.321   1.368   1.443
107   G         1.236   1.271   1.321   1.530   1.578   1.653   1.321   1.368   1.443
108   K         1.236   1.271   1.321   0.584   0.630   0.756   1.321   1.368   1.443
109   K         1.236   1.271   1.321   0.584   0.630   0.756   1.321   1.368   1.443
110   F         1.236   1.271   1.321   1.629   1.678   1.747   1.321   1.368   1.443
111   I        -0.103  -0.057   0.039  -0.006   0.071   0.202  -0.471  -0.395  -0.250
112   A        -1.337  -1.292  -1.222  -1.078  -1.004  -0.939  -1.260  -1.187  -1.078
113   D         1.236   1.271   1.321   1.450   1.509   1.577   1.321   1.368   1.443
114   N         0.006   0.072   0.069   0.261   0.355   0.350  -0.272  -0.162  -0.195
115   D         1.236   1.271   1.321   1.450   1.509   1.577   1.321   1.368   1.443
116   T        -1.779  -1.760  -1.645  -1.344  -1.290  -1.189  -1.467  -1.423  -1.260
117   L        -0.440  -0.525  -0.630  -0.085  -0.173  -0.319  -0.471  -0.593  -0.710
118   T        -0.440  -0.525  -0.630  -0.035  -0.154  -0.355  -0.471  -0.593  -0.710
119   L         0.366   0.486   0.194   0.627   0.793   0.419   0.325   0.505   0.027
120   N         0.269   0.242   0.116   0.635   0.610   0.427   0.174   0.127  -0.110
121   R         1.236   1.271   1.321   1.253   1.306   1.390   1.321   1.368   1.443
122   L         1.236   1.271   1.321   1.356   1.422   1.488   1.321   1.368   1.443
123   I        -0.103  -0.057   0.039  -0.006   0.071   0.202  -0.471  -0.395  -0.250
124   T        -0.440  -0.513  -0.622  -0.923  -1.024  -1.054  -0.471  -0.580  -0.700
125   T        -0.718  -0.786  -0.681  -0.486  -0.542  -0.429  -0.870  -0.926  -0.778
126   I         0.366   0.180   0.148   0.726   0.475   0.398   0.325   0.023  -0.054
127   G        -0.103  -0.057   0.039   0.012   0.075   0.217  -0.471  -0.395  -0.250
128   D         0.366   0.180   0.148   0.636   0.354   0.256   0.325   0.023  -0.054
129   K         1.236   1.271   1.321   0.584   0.630   0.756   1.321   1.368   1.443
130   Y        -0.440  -0.525  -0.630   0.143   0.011  -0.178  -0.471  -0.593  -0.710
131   D         1.236   1.271   1.321   1.450   1.509   1.577   1.321   1.368   1.443
132   D        -1.333  -1.331  -1.235  -1.071  -1.062  -0.966  -1.268  -1.241  -1.091
133   I        -1.164  -1.180  -1.204  -0.767  -0.826  -0.885  -1.069  -1.080  -1.060
134   A        -0.718  -0.596  -0.664  -0.482  -0.300  -0.417  -0.870  -0.705  -0.759
135   E         0.366   0.180   0.148   0.429   0.157   0.101   0.325   0.023  -0.054
136   S         1.236   1.271   1.321   1.356   1.418   1.488   1.321   1.368   1.443
137   K         1.236   1.271   1.321   0.584   0.630   0.756   1.321   1.368   1.443
138   L         1.236   1.271   1.321   1.356   1.422   1.488   1.321   1.368   1.443
139   E         1.236   1.271   1.321   1.220   1.267   1.359   1.321   1.368   1.443
140   K         1.236   1.271   1.321   0.584   0.630   0.756   1.321   1.368   1.443
141   A        -1.333  -1.249  -1.218  -1.111  -0.981  -0.925  -1.268  -1.158  -1.075
142   R         1.236   1.271   1.321   1.269   1.330   1.405   1.321   1.368   1.443
143   R         0.366   0.180   0.148   0.508   0.270   0.208   0.325   0.023  -0.054
144   L         1.236   1.271   1.321   1.356   1.422   1.488   1.321   1.368   1.443
145   E         0.366   0.444   0.186   0.453   0.558   0.212   0.325   0.444   0.012
146   E        -0.440  -0.592  -0.634  -0.218  -0.398  -0.433  -0.471  -0.674  -0.716
147   I         1.236   1.271   1.321   1.492   1.549   1.617   1.321   1.368   1.443
148   R         1.236   1.271   1.321   1.269   1.330   1.405   1.321   1.368   1.443
149   E         0.006   0.119   0.084  -0.065   0.109   0.053  -0.272  -0.080  -0.168
150   L         0.366   0.180   0.148   0.593   0.357   0.283   0.325   0.023  -0.054
151   K         1.236   1.271   1.321   0.584   0.630   0.756   1.321   1.368   1.443
152   R         0.366   0.444   0.186   0.336   0.469  -0.073   0.325   0.444   0.012
153   K         0.006  -0.021   0.045  -0.202  -0.342  -0.321  -0.272  -0.329  -0.238
154   E         1.236   1.271   1.321   1.220   1.267   1.359   1.321   1.368   1.443
155   I        -0.440  -0.298  -0.580   0.011   0.209  -0.198  -0.471  -0.274  -0.645
156   E         1.236   1.271   1.321   1.220   1.267   1.359   1.321   1.368   1.443
157   R         0.366   0.180   0.148   0.336  -0.020  -0.169   0.325   0.023  -0.054
158   K         0.366   0.180   0.148  -0.230  -0.496  -0.504   0.325   0.023  -0.054
159   E         1.236   1.271   1.321   1.220   1.267   1.359   1.321   1.368   1.443
160   A        -1.164  -0.978  -1.169  -0.876  -0.608  -0.895  -1.069  -0.838  -1.024
161   L        -1.333  -1.241  -1.218  -1.188  -1.073  -1.039  -1.268  -1.151  -1.075
162   K         1.236   1.271   1.321   0.584   0.630   0.756   1.321   1.368   1.443
163   Q        -0.886  -0.825  -0.711  -1.376  -1.223  -1.089  -1.069  -0.974  -0.815
164   D        -1.779  -1.760  -1.645  -1.436  -1.420  -1.280  -1.467  -1.423  -1.260
165   K        -0.718  -0.800  -0.682  -1.270  -1.362  -1.104  -0.870  -0.944  -0.781
166   L         0.366   0.180   0.148   0.596   0.361   0.289   0.325   0.023  -0.054
167   E         1.236   1.271   1.321   1.220   1.267   1.359   1.321   1.368   1.443
168   E        -0.718  -0.797  -0.684  -0.534  -0.633  -0.496  -0.870  -0.940  -0.783
169   K         1.236   1.271   1.321   0.584   0.630   0.756   1.321   1.368   1.443
170   K         1.236   1.271   1.321   0.584   0.630   0.756   1.321   1.368   1.443
171   D        -0.440  -0.552  -0.626  -0.244  -0.536  -0.678  -0.471  -0.625  -0.705
172   E         1.236   1.271   1.321   1.220   1.267   1.359   1.321   1.368   1.443
173   I        -0.718  -0.629  -0.665  -0.494  -0.343  -0.421  -0.870  -0.741  -0.761
174   K         0.366   0.486   0.194  -0.230  -0.069  -0.436   0.325   0.505   0.027
175   K         0.006   0.102   0.095  -0.708  -0.571  -0.523  -0.272  -0.111  -0.149
176   K         0.366   0.180   0.148  -0.175  -0.401  -0.384   0.325   0.023  -0.054
177   S        -0.103  -0.051   0.041  -0.208  -0.109   0.018  -0.471  -0.384  -0.245
178   S         0.366   0.180   0.148   0.552   0.286   0.194   0.325   0.023  -0.054
179   V        -1.164  -1.237  -1.201  -0.694  -0.788  -0.768  -1.069  -1.137  -1.057
180   A         0.366   0.180   0.148   0.398   0.036  -0.111   0.325   0.023  -0.054
181   R         0.366   0.180   0.148   0.482   0.230   0.155   0.325   0.023  -0.054
182   T        -0.886  -0.824  -0.712  -0.823  -0.725  -0.578  -1.069  -0.974  -0.816
183   I        -1.779  -1.757  -1.650  -1.156  -1.123  -0.987  -1.467  -1.421  -1.264
184   R         0.366   0.180   0.148   0.336  -0.020  -0.169   0.325   0.023  -0.054
185   R         0.366   0.180   0.148   0.507   0.267   0.207   0.325   0.023  -0.054
186   K         0.366   0.180   0.148  -0.179  -0.411  -0.392   0.325   0.023  -0.054
187   N        -1.164  -1.185  -1.196  -0.970  -1.165  -1.142  -1.069  -1.084  -1.052
188   K        -0.718  -0.649  -0.660  -1.362  -1.279  -1.214  -0.870  -0.758  -0.751
189   R        -0.718  -0.786  -0.681  -1.056  -1.049  -0.930  -0.870  -0.926  -0.778
190   D        -1.779  -1.724  -1.651  -1.429  -1.388  -1.283  -1.467  -1.395  -1.265
191   M        -1.333  -1.203  -1.211  -1.108  -0.957  -0.923  -1.268  -1.120  -1.069
192   L        -1.164  -1.042  -1.178  -1.194  -0.963  -1.361  -1.069  -0.912  -1.033
193   K        -1.164  -1.042  -1.178  -1.653  -1.495  -1.552  -1.069  -0.912  -1.033
194   S        -1.779  -1.722  -1.640  -1.708  -1.803  -1.576  -1.467  -1.393  -1.256
195   E        -0.440  -0.592  -0.634  -0.506  -0.866  -0.955  -0.471  -0.674  -0.716
196   A        -1.442  -1.398  -1.250  -1.158  -1.082  -0.895  -1.368  -1.296  -1.105
197   K        -1.442  -1.398  -1.250  -1.688  -1.563  -1.397  -1.368  -1.296  -1.105
198   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
199   A        -0.772  -0.776  -0.698  -0.701  -0.707  -0.617  -0.919  -0.910  -0.799
200   S        -1.164  -1.158  -1.190  -0.904  -0.934  -0.976  -1.069  -1.057  -1.046
201   E        -1.164  -1.048  -1.164  -1.177  -0.993  -1.233  -1.069  -0.918  -1.019
202   S        -1.779  -1.757  -1.650  -1.446  -1.369  -1.274  -1.467  -1.421  -1.264
203   K        -0.718  -0.598  -0.658  -1.420  -1.270  -1.249  -0.870  -0.694  -0.751
204   T        -1.779  -1.741  -1.656  -1.245  -1.173  -1.054  -1.467  -1.409  -1.269
205   E        -1.779  -1.615  -1.625  -1.279  -1.042  -1.081  -1.467  -1.286  -1.244
206   G        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
207   R        -0.718  -0.797  -0.684  -1.375  -1.426  -1.206  -0.870  -0.940  -0.783
208   K         0.366   0.444   0.186  -0.166  -0.067  -0.323   0.325   0.444   0.012
209   V         0.366   0.492   0.193  -0.180  -0.022  -0.340   0.325   0.515   0.025
210   K         1.236   1.271   1.321   0.584   0.630   0.756   1.321   1.368   1.443
211   K         0.006   0.126   0.084  -0.326  -0.049  -0.275  -0.272  -0.068  -0.167
212   V         1.236   1.271   1.321   1.475   1.527   1.601   1.321   1.368   1.443
213   S         1.236   1.271   1.321   1.356   1.418   1.488   1.321   1.368   1.443
214   F         1.236   1.271   1.321   1.629   1.678   1.747   1.321   1.368   1.443
215   A         0.366   0.486   0.194   0.567   0.728   0.331   0.325   0.505   0.027
216   Q        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P43586
Output conservation file - output/P43586
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
