1     M         1.814   1.904   2.014   2.007   2.141   2.321   2.002   2.154   2.349
2     E        -1.549  -1.475  -1.438  -1.579  -1.472  -1.447  -1.231  -1.149  -1.061
3     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
4     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
5     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
6     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
7     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
8     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
9     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
10    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
11    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
12    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
13    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
14    A        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
15    D         0.268   0.202   0.066   0.307   0.196   0.013   0.216   0.104  -0.109
16    W         0.722   0.664   0.720   0.958   0.858   0.917   0.510   0.367   0.406
17    R         1.814   1.904   2.014   1.851   1.982   2.162   2.002   2.154   2.349
18    R         0.067   0.054  -0.009   0.092   0.009  -0.073  -0.112  -0.188  -0.241
19    I         1.083   0.941   0.932   1.130   0.915   0.891   1.132   0.887   0.830
20    D         1.083   1.027   0.943   1.104   1.063   0.949   1.132   1.037   0.851
21    I         0.722   0.664   0.720   0.608   0.455   0.515   0.510   0.367   0.406
22    D         1.814   1.904   2.014   1.717   1.858   2.025   2.002   2.154   2.349
23    A         0.386   0.238   0.126   0.449   0.221   0.057   0.386   0.165  -0.017
24    F        -0.750  -0.764  -0.637  -0.832  -0.854  -0.647  -0.858  -0.843  -0.693
25    D        -0.204  -0.185  -0.095  -0.418  -0.400  -0.281  -0.485  -0.474  -0.358
26    P         0.067   0.055  -0.010   0.125   0.039  -0.027  -0.112  -0.187  -0.242
27    E        -0.077  -0.125  -0.021  -0.256  -0.280  -0.140  -0.360  -0.385  -0.254
28    S         0.268   0.426   0.098   0.302   0.511   0.059   0.216   0.452  -0.059
29    G        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
30    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
31    R        -1.086  -1.193  -1.089  -0.775  -0.939  -0.758  -0.982  -1.056  -0.923
32    L         0.386   0.315   0.140   0.332   0.262   0.025   0.386   0.286   0.005
33    T        -0.540  -0.504  -0.543  -0.549  -0.485  -0.570  -0.609  -0.550  -0.584
34    A        -1.086  -1.140  -1.067  -1.015  -1.101  -1.002  -0.982  -1.011  -0.903
35    A        -0.877  -0.943  -0.996  -0.714  -0.849  -0.957  -0.733  -0.807  -0.828
36    D         1.083   1.027   0.943   1.083   1.037   0.904   1.132   1.037   0.851
37    L         1.083   0.941   0.932   0.921   0.742   0.727   1.132   0.887   0.830
38    V        -1.885  -1.870  -1.771  -1.656  -1.536  -1.464  -1.355  -1.321  -1.185
39    P         1.814   1.904   2.014   1.864   2.000   2.175   2.002   2.154   2.349
40    P        -0.030  -0.156  -0.081  -0.027  -0.267  -0.175  -0.226  -0.425  -0.334
41    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
42    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
43    Y        -0.750  -0.794  -0.643  -0.781  -0.820  -0.613  -0.858  -0.881  -0.701
44    E        -1.549  -1.420  -1.413  -1.514  -1.328  -1.298  -1.231  -1.105  -1.042
45    T        -0.877  -0.868  -0.973  -0.540  -0.557  -0.687  -0.733  -0.722  -0.804
46    T        -0.750  -0.658  -0.641  -0.791  -0.678  -0.647  -0.858  -0.757  -0.698
47    V        -0.270  -0.428  -0.505  -0.205  -0.450  -0.559  -0.236  -0.442  -0.526
48    T        -0.540  -0.517  -0.535  -0.616  -0.593  -0.621  -0.609  -0.562  -0.573
49    L        -1.549  -1.584  -1.463  -1.557  -1.600  -1.450  -1.231  -1.232  -1.080
50    Q        -0.286  -0.157  -0.078  -0.556  -0.373  -0.253  -0.609  -0.428  -0.331
51    E        -0.270  -0.428  -0.505  -0.096  -0.362  -0.487  -0.236  -0.442  -0.526
52    L        -1.213  -1.205  -1.080  -1.467  -1.453  -1.260  -1.106  -1.068  -0.917
53    Q        -0.270  -0.427  -0.506  -0.141  -0.374  -0.552  -0.236  -0.441  -0.527
54    P        -1.086  -0.975  -1.022  -1.026  -0.855  -0.949  -0.982  -0.845  -0.858
55    R        -1.213  -1.173  -1.077  -1.402  -1.373  -1.221  -1.106  -1.047  -0.914
56    M        -1.549  -1.455  -1.418  -1.424  -1.298  -1.226  -1.231  -1.134  -1.046
57    N        -1.549  -1.517  -1.425  -1.286  -1.236  -1.112  -1.231  -1.182  -1.051
58    Q         1.083   1.143   0.960   1.152   1.253   1.001   1.132   1.230   0.882
59    L        -1.086  -1.161  -1.078  -1.188  -1.332  -1.124  -0.982  -1.035  -0.914
60    R         1.814   1.904   2.014   1.851   1.982   2.162   2.002   2.154   2.349
61    S         0.386   0.315   0.140   0.444   0.357   0.094   0.386   0.286   0.005
62    L        -1.086  -0.911  -1.008  -1.061  -0.862  -0.924  -0.982  -0.797  -0.851
63    A        -0.750  -0.654  -0.633  -1.057  -0.929  -0.868  -0.858  -0.752  -0.690
64    T        -0.270  -0.416  -0.498  -0.158  -0.374  -0.495  -0.236  -0.429  -0.517
65    S        -0.877  -1.016  -1.022  -0.795  -1.004  -1.058  -0.733  -0.880  -0.855
66    G         1.814   1.904   2.014   1.806   1.936   2.117   2.002   2.154   2.349
67    D        -0.540  -0.592  -0.571  -0.546  -0.574  -0.568  -0.609  -0.644  -0.613
68    S        -0.623  -0.436  -0.566  -0.587  -0.375  -0.517  -0.733  -0.512  -0.616
69    L        -1.086  -1.042  -1.008  -1.241  -1.163  -1.109  -0.982  -0.911  -0.844
70    G        -0.286  -0.221  -0.118  -0.537  -0.441  -0.304  -0.609  -0.521  -0.390
71    A         1.083   0.941   0.932   1.093   0.896   0.899   1.132   0.887   0.830
72    V         0.530   0.680   0.742   0.018   0.270   0.336   0.137   0.398   0.451
73    Q        -1.885  -1.870  -1.771  -1.639  -1.578  -1.479  -1.355  -1.321  -1.185
74    L        -0.270  -0.502  -0.539  -0.312  -0.637  -0.704  -0.236  -0.525  -0.569
75    L        -1.086  -1.049  -1.047  -1.064  -1.041  -0.977  -0.982  -0.939  -0.887
76    T         0.530   0.680   0.742   0.092   0.335   0.407   0.137   0.398   0.451
77    T        -0.750  -0.760  -0.638  -0.938  -0.945  -0.782  -0.858  -0.847  -0.696
78    D        -1.422  -1.479  -1.447  -1.069  -1.098  -1.034  -1.106  -1.145  -1.067
79    P         0.067   0.040   0.007   0.060  -0.017  -0.097  -0.112  -0.166  -0.216
80    P         1.083   1.027   0.943   1.212   1.151   1.011   1.132   1.037   0.851
81    Y         1.814   1.904   2.014   1.938   2.076   2.251   2.002   2.154   2.349
82    S        -1.213  -1.153  -1.084  -1.241  -1.159  -1.063  -1.106  -1.027  -0.920
83    A        -0.750  -0.797  -0.647  -0.891  -0.929  -0.724  -0.858  -0.884  -0.706
84    D         1.083   1.027   0.943   1.069   1.018   0.875   1.132   1.037   0.851
85    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
86    A        -0.877  -0.869  -0.964  -0.714  -0.723  -0.900  -0.733  -0.723  -0.794
87    P        -1.549  -1.481  -1.424  -1.440  -1.355  -1.268  -1.231  -1.158  -1.051
88    T        -0.204  -0.237  -0.122  -0.439  -0.550  -0.388  -0.485  -0.539  -0.394
89    K         1.083   0.941   0.932   1.229   1.067   1.040   1.132   0.887   0.830
90    E        -0.877  -0.878  -0.971  -0.679  -0.698  -0.859  -0.733  -0.733  -0.802
91    Q        -0.750  -0.689  -0.623  -0.983  -0.855  -0.794  -0.858  -0.772  -0.679
92    Y         0.469   0.533   0.662   0.464   0.561   0.753   0.013   0.100   0.284
93    F        -0.204  -0.005  -0.000  -0.511  -0.215  -0.218  -0.485  -0.191  -0.211
94    K        -1.422  -1.428  -1.396  -1.131  -1.091  -1.117  -1.106  -1.103  -1.023
95    S        -0.750  -0.671  -0.638  -0.912  -0.821  -0.764  -0.858  -0.770  -0.697
96    V         0.386   0.326   0.149   0.443   0.366   0.143   0.386   0.283   0.019
97    L        -0.750  -0.797  -0.656  -0.941  -1.020  -0.785  -0.858  -0.884  -0.714
98    E         1.083   0.941   0.932   1.161   0.965   0.950   1.132   0.887   0.830
99    A         0.722   0.899   0.839   0.499   0.758   0.652   0.510   0.812   0.648
100   L         1.814   1.904   2.014   1.555   1.689   1.862   2.002   2.154   2.349
101   T        -1.213  -1.158  -1.085  -1.093  -1.042  -0.929  -1.106  -1.030  -0.920
102   Q        -0.623  -0.690  -0.611  -0.721  -0.784  -0.674  -0.733  -0.778  -0.664
103   V        -0.077   0.022   0.000  -0.290  -0.115  -0.135  -0.360  -0.198  -0.222
104   R         0.469   0.533   0.662   0.265   0.365   0.551   0.013   0.100   0.284
105   Q        -1.213  -1.103  -1.082  -1.198  -1.065  -1.026  -1.106  -0.978  -0.917
106   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
107   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
108   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
109   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
110   A        -1.086  -0.980  -1.010  -0.992  -0.874  -0.885  -0.982  -0.851  -0.846
111   D         0.722   0.711   0.733   0.574   0.545   0.544   0.510   0.458   0.434
112   I         0.067   0.097   0.053   0.010   0.098   0.001  -0.112  -0.041  -0.135
113   G        -1.213  -1.086  -1.075  -1.233  -1.036  -1.027  -1.106  -0.977  -0.913
114   N        -0.877  -1.017  -1.022  -0.547  -0.798  -0.827  -0.733  -0.880  -0.855
115   V        -0.750  -0.721  -0.632  -0.686  -0.597  -0.510  -0.858  -0.795  -0.688
116   I         0.386   0.417   0.173   0.367   0.387   0.035   0.386   0.426   0.056
117   K         0.386   0.404   0.163   0.595   0.639   0.276   0.386   0.408   0.040
118   N        -1.549  -1.513  -1.424  -1.398  -1.353  -1.220  -1.231  -1.180  -1.050
119   L         0.386   0.138   0.098   0.346   0.047  -0.006   0.386   0.024  -0.059
120   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
121   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
122   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
123   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
124   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
125   S        -0.750  -0.724  -0.620  -0.866  -0.808  -0.671  -0.858  -0.800  -0.676
126   D        -1.086  -1.081  -1.052  -1.102  -1.070  -1.029  -0.982  -0.954  -0.887
127   S        -0.540  -0.291  -0.512  -0.490  -0.123  -0.420  -0.609  -0.280  -0.540
128   Q         0.386   0.138   0.098   0.574   0.261   0.207   0.386   0.024  -0.059
129   R        -0.877  -0.944  -0.997  -0.749  -0.889  -1.001  -0.733  -0.808  -0.829
130   D         1.814   1.904   2.014   1.717   1.858   2.025   2.002   2.154   2.349
131   V        -0.270  -0.258  -0.453  -0.188  -0.180  -0.448  -0.236  -0.231  -0.461
132   L         1.083   0.941   0.932   0.921   0.742   0.727   1.132   0.887   0.830
133   V         0.067   0.047   0.020  -0.024  -0.035  -0.073  -0.112  -0.121  -0.192
134   K         1.083   0.941   0.932   1.241   1.086   1.068   1.132   0.887   0.830
135   Y         1.083   0.941   0.932   1.333   1.175   1.167   1.132   0.887   0.830
136   L         1.083   0.941   0.932   0.921   0.742   0.727   1.132   0.887   0.830
137   Y         1.814   1.904   2.014   1.938   2.076   2.251   2.002   2.154   2.349
138   K         0.386   0.325   0.152   0.628   0.554   0.321   0.386   0.282   0.023
139   G         0.067   0.194   0.069   0.107   0.302   0.107  -0.112   0.095  -0.110
140   M         1.083   0.941   0.932   1.340   1.140   1.101   1.132   0.887   0.830
141   S         0.067  -0.106  -0.029  -0.041  -0.319  -0.215  -0.112  -0.353  -0.267
142   V        -1.549  -1.552  -1.449  -1.551  -1.538  -1.384  -1.231  -1.210  -1.069
143   P         0.386   0.405   0.165   0.590   0.627   0.261   0.386   0.410   0.044
144   Q        -0.750  -0.694  -0.603  -0.785  -0.689  -0.577  -0.858  -0.762  -0.651
145   G         0.722   0.620   0.703   0.635   0.409   0.520   0.510   0.278   0.371
146   Q        -0.270  -0.427  -0.506  -0.090  -0.308  -0.457  -0.236  -0.441  -0.527
147   K         0.067  -0.106  -0.029   0.105  -0.147  -0.074  -0.112  -0.353  -0.267
148   Q        -0.270  -0.426  -0.509  -0.070  -0.318  -0.424  -0.236  -0.440  -0.530
149   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
150   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
151   G         1.814   1.904   2.014   1.793   1.913   2.104   2.002   2.154   2.349
152   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
153   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
154   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
155   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
156   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
157   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
158   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
159   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
160   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
161   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
162   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
163   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
164   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
165   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
166   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
167   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
168   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
169   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
170   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
171   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
172   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
173   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
174   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
175   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
176   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
177   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
178   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
179   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
180   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
181   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
182   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
183   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
184   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
185   G         0.067  -0.020  -0.030   0.018  -0.184  -0.197  -0.112  -0.272  -0.271
186   V        -0.286  -0.178  -0.079  -0.704  -0.546  -0.407  -0.609  -0.461  -0.337
187   L         1.814   1.904   2.014   1.555   1.689   1.862   2.002   2.154   2.349
188   L         1.814   1.904   2.014   1.555   1.689   1.862   2.002   2.154   2.349
189   A        -0.540  -0.574  -0.587  -0.465  -0.547  -0.496  -0.609  -0.654  -0.639
190   W         1.814   1.904   2.014   2.054   2.193   2.369   2.002   2.154   2.349
191   L        -0.750  -0.709  -0.639  -0.632  -0.517  -0.441  -0.858  -0.799  -0.697
192   E         1.814   1.904   2.014   1.806   1.927   2.117   2.002   2.154   2.349
193   R         1.083   1.257   0.978   1.229   1.473   1.113   1.132   1.407   0.917
194   I        -0.750  -0.658  -0.641  -0.959  -0.863  -0.850  -0.858  -0.757  -0.698
195   T        -0.077   0.099   0.020  -0.317  -0.065  -0.145  -0.360  -0.105  -0.192
196   Q        -0.077   0.022   0.000  -0.226  -0.053  -0.092  -0.360  -0.198  -0.222
197   V         0.067   0.087   0.009  -0.066  -0.093  -0.167  -0.112  -0.127  -0.213
198   S        -0.750  -0.696  -0.643  -0.877  -0.843  -0.730  -0.858  -0.800  -0.704
199   G         1.814   1.904   2.014   1.806   1.936   2.117   2.002   2.154   2.349
200   V        -0.540  -0.663  -0.595  -0.739  -0.945  -0.858  -0.609  -0.741  -0.647
201   T        -0.270   0.007  -0.441  -0.024   0.349  -0.259  -0.236   0.135  -0.445
202   P        -0.540  -0.535  -0.591  -0.353  -0.339  -0.428  -0.609  -0.627  -0.644
203   I         1.083   1.143   0.960   1.145   1.224   0.973   1.132   1.230   0.882
204   V         0.386   0.404   0.168   0.397   0.415   0.084   0.386   0.408   0.048
205   H         0.530   0.680   0.742   0.484   0.713   0.803   0.137   0.398   0.451
206   Y        -0.204  -0.185  -0.104  -0.200  -0.245  -0.104  -0.485  -0.474  -0.369
207   I        -0.286  -0.190  -0.081  -0.517  -0.378  -0.231  -0.609  -0.484  -0.341
208   S        -0.540  -0.600  -0.589  -0.571  -0.651  -0.635  -0.609  -0.678  -0.640
209   D         1.083   0.941   0.932   1.069   0.891   0.856   1.132   0.887   0.830
210   R         1.083   1.027   0.943   1.246   1.198   1.099   1.132   1.037   0.851
211   R         1.083   0.941   0.932   1.226   1.052   1.040   1.132   0.887   0.830
212   T         0.386   0.138   0.098   0.534   0.196   0.107   0.386   0.024  -0.059
213   V         1.083   0.941   0.932   0.998   0.786   0.759   1.132   0.887   0.830
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P40518
Output conservation file - output/P40518
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
