1     M         1.700   1.700   1.722   1.930   1.929   1.965   1.808   1.808   1.836
2     T        -1.030  -1.030  -1.013  -0.755  -0.756  -0.726  -0.924  -0.924  -0.893
3     S         0.118   0.112   0.080   0.160   0.149   0.078  -0.013  -0.023  -0.074
4     I        -0.022  -0.022  -0.004  -0.237  -0.237  -0.206  -0.241  -0.241  -0.211
5     N        -0.022  -0.022  -0.004  -0.214  -0.214  -0.183  -0.241  -0.241  -0.211
6     S         1.700   1.700   1.722   1.495   1.495   1.530   1.808   1.808   1.836
7     F        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
8     P        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
9     R        -0.022  -0.022  -0.004  -0.180  -0.180  -0.150  -0.241  -0.241  -0.211
10    N        -0.022  -0.022  -0.004  -0.388  -0.388  -0.358  -0.241  -0.241  -0.211
11    I         1.700   1.700   1.722   1.604   1.604   1.639   1.808   1.808   1.836
12    D        -1.030  -1.030  -1.013  -0.994  -0.994  -0.965  -0.924  -0.924  -0.893
13    W         1.700   1.700   1.722   1.990   1.990   2.026   1.808   1.808   1.836
14    P         1.700   1.700   1.722   1.950   1.950   1.985   1.808   1.808   1.836
15    S        -1.030  -1.030  -1.013  -0.961  -0.962  -0.933  -0.924  -0.924  -0.893
16    N        -1.030  -1.030  -1.013  -0.866  -0.869  -0.837  -0.924  -0.924  -0.893
17    I        -1.030  -1.030  -1.013  -1.007  -1.009  -0.978  -0.924  -0.924  -0.893
18    G        -1.030  -1.030  -1.013  -0.910  -0.908  -0.881  -0.924  -0.924  -0.893
19    I         1.700   1.700   1.722   1.639   1.639   1.674   1.808   1.808   1.836
20    K        -1.030  -1.030  -1.013  -0.885  -0.883  -0.856  -0.924  -0.924  -0.893
21    K        -1.030  -1.030  -1.013  -0.866  -0.864  -0.837  -0.924  -0.924  -0.893
22    I         0.118   0.112   0.080   0.222   0.211   0.148  -0.013  -0.023  -0.074
23    E         0.118   0.112   0.080   0.141   0.132   0.077  -0.013  -0.023  -0.074
24    G        -0.022  -0.022  -0.004   0.112   0.112   0.143  -0.241  -0.241  -0.211
25    T        -1.030  -1.030  -1.013  -1.007  -1.006  -0.978  -0.924  -0.924  -0.893
26    N         0.118   0.115   0.084   0.156   0.151   0.113  -0.013  -0.019  -0.069
27    P        -1.030  -1.030  -1.013  -0.948  -0.948  -0.920  -0.924  -0.924  -0.893
28    T         1.700   1.700   1.722   1.734   1.735   1.769   1.808   1.808   1.836
29    V         0.118   0.128   0.084   0.049   0.064  -0.013  -0.013   0.002  -0.068
30    N        -1.030  -1.030  -1.013  -1.067  -1.066  -1.038  -0.924  -0.924  -0.893
31    A        -1.030  -1.030  -1.013  -0.835  -0.833  -0.806  -0.924  -0.924  -0.893
32    I         1.700   1.700   1.722   1.639   1.639   1.674   1.808   1.808   1.836
33    K        -1.030  -1.030  -1.013  -0.948  -0.949  -0.920  -0.924  -0.924  -0.893
34    G        -1.030  -1.030  -1.013  -1.121  -1.118  -1.092  -0.924  -0.924  -0.893
35    L         0.118   0.128   0.084  -0.080  -0.064  -0.139  -0.013   0.002  -0.068
36    L         0.118   0.112   0.080   0.170   0.158   0.090  -0.013  -0.023  -0.074
37    Y         0.118   0.128   0.084   0.160   0.179   0.085  -0.013   0.002  -0.068
38    N         0.118   0.115   0.084   0.189   0.184   0.150  -0.013  -0.019  -0.069
39    G         0.118   0.112   0.080   0.203   0.194   0.148  -0.013  -0.023  -0.074
40    G         1.700   1.700   1.722   1.869   1.868   1.904   1.808   1.808   1.836
41    S        -1.030  -1.030  -1.013  -1.246  -1.245  -1.218  -0.924  -0.924  -0.893
42    I        -1.030  -1.030  -1.013  -1.121  -1.119  -1.092  -0.924  -0.924  -0.893
43    Y        -1.030  -1.030  -1.013  -0.816  -0.818  -0.787  -0.924  -0.924  -0.893
44    A        -1.030  -1.030  -1.013  -0.974  -0.973  -0.946  -0.924  -0.924  -0.893
45    F        -1.030  -1.030  -1.013  -0.816  -0.815  -0.787  -0.924  -0.924  -0.893
46    L        -1.030  -1.030  -1.013  -1.040  -1.044  -1.011  -0.924  -0.924  -0.893
47    Y         1.700   1.700   1.722   1.869   1.869   1.904   1.808   1.808   1.836
48    F         0.118   0.112   0.080   0.151   0.141   0.097  -0.013  -0.023  -0.074
49    V        -1.030  -1.030  -1.013  -0.791  -0.794  -0.762  -0.924  -0.924  -0.893
50    I         0.118   0.128   0.084   0.030   0.044  -0.029  -0.013   0.002  -0.068
51    A        -1.030  -1.030  -1.013  -0.797  -0.793  -0.768  -0.924  -0.924  -0.893
52    M        -1.030  -1.030  -1.013  -0.936  -0.935  -0.907  -0.924  -0.924  -0.893
53    F        -1.030  -1.030  -1.013  -0.955  -0.955  -0.926  -0.924  -0.924  -0.893
54    V         0.118   0.115   0.084   0.005  -0.001  -0.064  -0.013  -0.019  -0.069
55    E        -1.030  -1.030  -1.013  -1.093  -1.094  -1.065  -0.924  -0.924  -0.893
56    P         0.118   0.115   0.084   0.292   0.285   0.222  -0.013  -0.019  -0.069
57    T        -1.030  -1.030  -1.013  -1.121  -1.118  -1.092  -0.924  -0.924  -0.893
58    L         1.700   1.700   1.722   1.543   1.544   1.577   1.808   1.808   1.836
59    Q        -1.030  -1.030  -1.013  -1.093  -1.094  -1.065  -0.924  -0.924  -0.893
60    K         0.118   0.128   0.084   0.093   0.110   0.029  -0.013   0.002  -0.068
61    Q         0.118   0.115   0.084   0.088   0.082   0.025  -0.013  -0.019  -0.069
62    Y         1.700   1.700   1.722   1.869   1.869   1.904   1.808   1.808   1.836
63    Q        -1.030  -1.030  -1.013  -0.994  -0.993  -0.965  -0.924  -0.924  -0.893
64    Q         1.700   1.700   1.722   1.724   1.723   1.759   1.808   1.808   1.836
65    R         1.700   1.700   1.722   1.629   1.629   1.663   1.808   1.808   1.836
66    N        -1.030  -1.030  -1.013  -1.182  -1.183  -1.154  -0.924  -0.924  -0.893
67    D         0.118   0.112   0.080   0.151   0.142   0.088  -0.013  -0.023  -0.074
68    F         0.118   0.112   0.080   0.025   0.016  -0.025  -0.013  -0.023  -0.074
69    S         0.118   0.128   0.084  -0.075  -0.060  -0.126  -0.013   0.002  -0.068
70    L        -1.030  -1.030  -1.013  -0.773  -0.775  -0.744  -0.924  -0.924  -0.893
71    F        -1.030  -1.030  -1.013  -0.773  -0.774  -0.744  -0.924  -0.924  -0.893
72    V         0.118   0.128   0.084   0.175   0.188   0.129  -0.013   0.002  -0.068
73    L         1.700   1.700   1.722   1.543   1.544   1.577   1.808   1.808   1.836
74    L         0.118   0.115   0.084   0.059   0.055   0.018  -0.013  -0.019  -0.069
75    R         0.118   0.128   0.084   0.044   0.060  -0.011  -0.013   0.002  -0.068
76    L        -1.030  -1.030  -1.013  -1.155  -1.156  -1.127  -0.924  -0.924  -0.893
77    R         1.700   1.700   1.722   1.629   1.629   1.663   1.808   1.808   1.836
78    R         1.700   1.700   1.722   1.629   1.629   1.663   1.808   1.808   1.836
79    I         0.118   0.112   0.080  -0.080  -0.090  -0.145  -0.013  -0.023  -0.074
80    I        -1.030  -1.030  -1.013  -1.033  -1.035  -1.005  -0.924  -0.924  -0.893
81    A         0.118   0.112   0.080   0.137   0.128   0.087  -0.013  -0.023  -0.074
82    Q        -1.030  -1.030  -1.013  -1.093  -1.095  -1.065  -0.924  -0.924  -0.893
83    L         1.700   1.700   1.722   1.543   1.544   1.577   1.808   1.808   1.836
84    Q        -1.030  -1.030  -1.013  -0.942  -0.940  -0.913  -0.924  -0.924  -0.893
85    K        -1.030  -1.030  -1.013  -1.155  -1.154  -1.127  -0.924  -0.924  -0.893
86    R         1.700   1.700   1.722   1.629   1.629   1.663   1.808   1.808   1.836
87    L         0.118   0.115   0.084  -0.060  -0.065  -0.116  -0.013  -0.019  -0.069
88    V         0.118   0.112   0.080   0.117   0.107   0.050  -0.013  -0.023  -0.074
89    M         0.118   0.112   0.080   0.020   0.012  -0.024  -0.013  -0.023  -0.074
90    T         0.118   0.128   0.084   0.141   0.157   0.084  -0.013   0.002  -0.068
91    P        -1.030  -1.030  -1.013  -1.007  -1.005  -0.978  -0.924  -0.924  -0.893
92    V         0.118   0.128   0.084  -0.015   0.002  -0.086  -0.013   0.002  -0.068
93    S         0.118   0.115   0.084  -0.075  -0.080  -0.127  -0.013  -0.019  -0.069
94    S        -1.030  -1.030  -1.013  -0.974  -0.974  -0.946  -0.924  -0.924  -0.893
95    L         0.118   0.115   0.084  -0.060  -0.065  -0.116  -0.013  -0.019  -0.069
96    G         0.118   0.128   0.084   0.264   0.279   0.203  -0.013   0.002  -0.068
97    F        -1.030  -1.030  -1.013  -0.968  -0.965  -0.939  -0.924  -0.924  -0.893
98    N         0.118   0.128   0.084   0.005   0.021  -0.057  -0.013   0.002  -0.068
99    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
100   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
101   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
102   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
103   -        -0.022  -0.022  -0.004  -0.242  -0.244  -0.211  -0.241  -0.241  -0.211
104   E         0.118   0.128   0.084   0.098   0.115   0.034  -0.013   0.002  -0.068
105   Q         0.118   0.112   0.080   0.137   0.127   0.072  -0.013  -0.023  -0.074
106   N        -1.030  -1.030  -1.013  -1.007  -1.008  -0.978  -0.924  -0.924  -0.893
107   N        -1.030  -1.030  -1.013  -1.033  -1.034  -1.005  -0.924  -0.924  -0.893
108   F         0.118   0.112   0.080   0.320   0.310   0.260  -0.013  -0.023  -0.074
109   V         0.118   0.128   0.084   0.175   0.188   0.129  -0.013   0.002  -0.068
110   E         0.118   0.112   0.080   0.160   0.151   0.096  -0.013  -0.023  -0.074
111   R         1.700   1.700   1.722   1.629   1.629   1.663   1.808   1.808   1.836
112   S         0.118   0.112   0.080   0.338   0.327   0.250  -0.013  -0.023  -0.074
113   T         0.118   0.115   0.084   0.137   0.131   0.078  -0.013  -0.019  -0.069
114   Q         1.700   1.700   1.722   1.724   1.723   1.759   1.808   1.808   1.836
115   T         1.700   1.700   1.722   1.734   1.735   1.769   1.808   1.808   1.836
116   -        -0.022  -0.022  -0.004   0.015   0.013   0.046  -0.241  -0.241  -0.211
117   -        -0.022  -0.022  -0.004  -0.312  -0.314  -0.281  -0.241  -0.241  -0.211
118   -        -0.022  -0.022  -0.004  -0.140  -0.141  -0.109  -0.241  -0.241  -0.211
119   -        -0.022  -0.022  -0.004  -0.173  -0.175  -0.143  -0.241  -0.241  -0.211
120   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
121   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
122   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
123   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
124   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
125   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
126   S         1.700   1.700   1.722   1.495   1.495   1.530   1.808   1.808   1.836
127   D         1.700   1.700   1.722   1.742   1.742   1.777   1.808   1.808   1.836
128   D         1.700   1.700   1.722   1.742   1.742   1.777   1.808   1.808   1.836
129   N        -0.022  -0.022  -0.004  -0.203  -0.203  -0.172  -0.241  -0.241  -0.211
130   I        -0.022  -0.022  -0.004  -0.037  -0.037  -0.006  -0.241  -0.241  -0.211
131   I        -0.022  -0.022  -0.004   0.049   0.049   0.080  -0.241  -0.241  -0.211
132   R        -0.022  -0.022  -0.004  -0.282  -0.283  -0.252  -0.241  -0.241  -0.211
133   E         0.118   0.128   0.084   0.151   0.167   0.094  -0.013   0.002  -0.068
134   -         1.700   1.700   1.722   1.474   1.472   1.508   1.808   1.808   1.836
135   -        -0.022  -0.022  -0.004  -0.173  -0.164  -0.143  -0.241  -0.241  -0.211
136   -         1.700   1.700   1.722   1.693   1.708   1.728   1.808   1.808   1.836
137   D        -1.030  -1.030  -1.013  -0.987  -0.986  -0.959  -0.924  -0.924  -0.893
138   N         0.118   0.128   0.084  -0.055  -0.037  -0.124  -0.013   0.002  -0.068
139   S        -1.030  -1.030  -1.013  -1.033  -1.033  -1.005  -0.924  -0.924  -0.893
140   H        -1.030  -1.030  -1.013  -0.689  -0.687  -0.659  -0.924  -0.924  -0.893
141   W         1.700   1.700   1.722   1.990   1.990   2.026   1.808   1.808   1.836
142   A        -1.030  -1.030  -1.013  -0.891  -0.889  -0.862  -0.924  -0.924  -0.893
143   E        -1.030  -1.030  -1.013  -1.067  -1.066  -1.038  -0.924  -0.924  -0.893
144   M         0.118   0.112   0.080   0.146   0.137   0.098  -0.013  -0.023  -0.074
145   I        -1.030  -1.030  -1.013  -1.210  -1.209  -1.182  -0.924  -0.924  -0.893
146   Y        -1.030  -1.030  -1.013  -0.974  -0.974  -0.946  -0.924  -0.924  -0.893
147   Q        -1.030  -1.030  -1.013  -1.014  -1.014  -0.985  -0.924  -0.924  -0.893
148   L         1.700   1.700   1.722   1.543   1.544   1.577   1.808   1.808   1.836
149   Q        -1.030  -1.030  -1.013  -1.040  -1.041  -1.011  -0.924  -0.924  -0.893
150   N        -1.030  -1.030  -1.013  -1.000  -1.001  -0.972  -0.924  -0.924  -0.893
151   M        -1.030  -1.030  -1.013  -0.968  -0.967  -0.939  -0.924  -0.924  -0.893
152   K         0.118   0.115   0.084   0.141   0.136   0.083  -0.013  -0.019  -0.069
153   Q         0.118   0.112   0.080   0.156   0.146   0.090  -0.013  -0.023  -0.074
154   E        -1.030  -1.030  -1.013  -1.121  -1.118  -1.092  -0.924  -0.924  -0.893
155   L         1.700   1.700   1.722   1.543   1.544   1.577   1.808   1.808   1.836
156   Q        -1.030  -1.030  -1.013  -1.073  -1.073  -1.045  -0.924  -0.924  -0.893
157   Y        -1.030  -1.030  -1.013  -0.981  -0.980  -0.952  -0.924  -0.924  -0.893
158   F         0.118   0.112   0.080   0.320   0.310   0.260  -0.013  -0.023  -0.074
159   N         0.118   0.115   0.084   0.189   0.184   0.150  -0.013  -0.019  -0.069
160   R        -1.030  -1.030  -1.013  -1.007  -1.007  -0.978  -0.924  -0.924  -0.893
161   S         0.118   0.115   0.084  -0.065  -0.069  -0.115  -0.013  -0.019  -0.069
162   S        -1.030  -1.030  -1.013  -0.761  -0.765  -0.732  -0.924  -0.924  -0.893
163   G        -1.030  -1.030  -1.013  -0.910  -0.910  -0.881  -0.924  -0.924  -0.893
164   Q        -1.030  -1.030  -1.013  -1.073  -1.073  -1.045  -0.924  -0.924  -0.893
165   P         0.118   0.115   0.084   0.297   0.290   0.227  -0.013  -0.019  -0.069
166   S         1.700   1.700   1.722   1.499   1.500   1.534   1.808   1.808   1.836
167   E        -0.022  -0.022  -0.004  -0.203  -0.203  -0.172  -0.241  -0.241  -0.211
168   S        -1.030  -1.030  -1.013  -1.134  -1.134  -1.106  -0.924  -0.924  -0.893
169   I        -1.030  -1.030  -1.013  -0.804  -0.807  -0.775  -0.924  -0.924  -0.893
170   D         0.118   0.112   0.080   0.151   0.142   0.088  -0.013  -0.023  -0.074
171   D        -1.030  -1.030  -1.013  -1.134  -1.133  -1.106  -0.924  -0.924  -0.893
172   F         0.118   0.112   0.080   0.025   0.016  -0.025  -0.013  -0.023  -0.074
173   V        -1.030  -1.030  -1.013  -1.121  -1.122  -1.092  -0.924  -0.924  -0.893
174   F         1.700   1.700   1.722   1.858   1.858   1.894   1.808   1.808   1.836
175   Q         0.118   0.115   0.084   0.132   0.125   0.074  -0.013  -0.019  -0.069
176   I        -1.030  -1.030  -1.013  -1.148  -1.146  -1.120  -0.924  -0.924  -0.893
177   K         1.700   1.700   1.722   1.734   1.734   1.769   1.808   1.808   1.836
178   M         0.118   0.112   0.080   0.059   0.051   0.014  -0.013  -0.023  -0.074
179   V         0.118   0.115   0.084   0.151   0.145   0.101  -0.013  -0.019  -0.069
180   T         0.118   0.128   0.084   0.117   0.133   0.056  -0.013   0.002  -0.068
181   D         1.700   1.700   1.722   1.755   1.756   1.790   1.808   1.808   1.836
182   Q         1.700   1.700   1.722   1.724   1.723   1.759   1.808   1.808   1.836
183   V         0.118   0.112   0.080  -0.060  -0.069  -0.122  -0.013  -0.023  -0.074
184   E        -1.030  -1.030  -1.013  -1.087  -1.084  -1.058  -0.924  -0.924  -0.893
185   L        -1.030  -1.030  -1.013  -1.080  -1.080  -1.052  -0.924  -0.924  -0.893
186   T        -1.030  -1.030  -1.013  -0.974  -0.976  -0.946  -0.924  -0.924  -0.893
187   D        -1.030  -1.030  -1.013  -1.073  -1.073  -1.045  -0.924  -0.924  -0.893
188   R        -1.030  -1.030  -1.013  -0.835  -0.834  -0.806  -0.924  -0.924  -0.893
189   S        -1.030  -1.030  -1.013  -1.040  -1.043  -1.011  -0.924  -0.924  -0.893
190   R        -1.030  -1.030  -1.013  -1.053  -1.054  -1.025  -0.924  -0.924  -0.893
191   A         0.118   0.112   0.080   0.222   0.213   0.169  -0.013  -0.023  -0.074
192   F        -1.030  -1.030  -1.013  -0.981  -0.979  -0.952  -0.924  -0.924  -0.893
193   S        -1.030  -1.030  -1.013  -1.007  -1.011  -0.978  -0.924  -0.924  -0.893
194   N         0.118   0.112   0.080   0.098   0.088   0.028  -0.013  -0.023  -0.074
195   K        -1.030  -1.030  -1.013  -0.974  -0.972  -0.946  -0.924  -0.924  -0.893
196   S         0.118   0.115   0.084  -0.010  -0.014  -0.053  -0.013  -0.019  -0.069
197   R         0.118   0.115   0.084  -0.065  -0.071  -0.135  -0.013  -0.019  -0.069
198   N         0.118   0.128   0.084   0.054   0.069  -0.001  -0.013   0.002  -0.068
199   I         0.118   0.128   0.084  -0.055  -0.038  -0.124  -0.013   0.002  -0.068
200   I         0.118   0.128   0.084   0.030   0.044  -0.029  -0.013   0.002  -0.068
201   Q         0.118   0.128   0.084   0.088   0.104   0.025  -0.013   0.002  -0.068
202   G        -1.030  -1.030  -1.013  -1.060  -1.059  -1.031  -0.924  -0.924  -0.893
203   I         0.118   0.115   0.084   0.030   0.023  -0.029  -0.013  -0.019  -0.069
204   R         1.700   1.700   1.722   1.629   1.629   1.663   1.808   1.808   1.836
205   E         1.700   1.700   1.722   1.734   1.735   1.769   1.808   1.808   1.836
206   I         0.118   0.112   0.080   0.278   0.266   0.203  -0.013  -0.023  -0.074
207   K         1.700   1.700   1.722   1.734   1.734   1.769   1.808   1.808   1.836
208   G         1.700   1.700   1.722   1.869   1.868   1.904   1.808   1.808   1.836
209   W         1.700   1.700   1.722   1.990   1.990   2.026   1.808   1.808   1.836
210   F         1.700   1.700   1.722   1.858   1.858   1.894   1.808   1.808   1.836
211   V         0.118   0.115   0.084   0.049   0.042  -0.013  -0.013  -0.019  -0.069
212   N         0.118   0.115   0.084   0.189   0.184   0.150  -0.013  -0.019  -0.069
213   G         1.700   1.700   1.722   1.869   1.868   1.904   1.808   1.808   1.836
214   Q        -1.030  -1.030  -1.013  -1.134  -1.133  -1.106  -0.924  -0.924  -0.893
215   V         0.118   0.115   0.084   0.049   0.042  -0.013  -0.013  -0.019  -0.069
216   P         0.118   0.128   0.084   0.236   0.254   0.159  -0.013   0.002  -0.068
217   R        -1.030  -1.030  -1.013  -1.127  -1.126  -1.099  -0.924  -0.924  -0.893
218   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P40155
Output conservation file - output/P40155
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
