1     M         0.717   0.724   0.769   0.983   0.992   1.026   0.764   0.773   0.832
2     K        -0.593  -0.589  -0.477  -0.908  -0.903  -0.745  -0.959  -0.952  -0.772
3     S         0.717   0.724   0.769   0.755   0.758   0.826   0.764   0.773   0.832
4     T        -2.242  -2.200  -2.129  -2.044  -1.962  -1.889  -1.993  -1.939  -1.750
5     F         0.717   0.724   0.769   0.709   0.707   0.786   0.764   0.773   0.832
6     K         0.717   0.724   0.769   0.510   0.512   0.612   0.764   0.773   0.832
7     S        -0.593  -0.589  -0.477  -0.810  -0.813  -0.629  -0.959  -0.952  -0.772
8     E        -0.029  -0.141  -0.431  -0.084  -0.212  -0.675  -0.040  -0.204  -0.691
9     Y        -1.342  -1.368  -1.210  -1.210  -1.289  -1.100  -1.533  -1.565  -1.318
10    P        -0.029  -0.141  -0.431   0.131  -0.020  -0.489  -0.040  -0.204  -0.691
11    F         0.717   0.724   0.769   0.709   0.707   0.786   0.764   0.773   0.832
12    E         0.717   0.724   0.769   0.569   0.598   0.664   0.764   0.773   0.832
13    K         0.717   0.724   0.769   0.510   0.512   0.612   0.764   0.773   0.832
14    R         0.717   0.724   0.769   0.738   0.761   0.811   0.764   0.773   0.832
15    K        -0.740  -0.671  -1.172  -0.743  -0.666  -1.281  -0.729  -0.638  -1.269
16    A        -0.029  -0.141  -0.431   0.156   0.021  -0.436  -0.040  -0.204  -0.691
17    E         0.717   0.724   0.769   0.569   0.598   0.664   0.764   0.773   0.832
18    S        -0.656  -0.638  -0.486  -0.863  -0.840  -0.589  -1.074  -1.042  -0.788
19    E        -1.409  -1.470  -1.711  -1.269  -1.331  -1.608  -1.303  -1.372  -1.571
20    R         0.717   0.724   0.769   0.738   0.761   0.811   0.764   0.773   0.832
21    I         0.717   0.724   0.769   0.610   0.610   0.700   0.764   0.773   0.832
22    A        -2.242  -2.255  -2.135  -2.086  -2.093  -1.921  -1.993  -1.986  -1.754
23    D        -1.685  -1.628  -1.711  -1.565  -1.520  -1.724  -1.648  -1.602  -1.572
24    R        -0.593  -0.550  -0.479  -0.991  -0.922  -0.778  -0.959  -0.881  -0.776
25    F        -0.593  -0.583  -0.481  -0.771  -0.765  -0.596  -0.959  -0.941  -0.780
26    K        -3.058  -3.057  -2.751  -2.707  -2.685  -2.219  -2.337  -2.324  -1.945
27    N        -0.593  -0.589  -0.477  -0.693  -0.688  -0.504  -0.959  -0.952  -0.772
28    R         0.717   0.724   0.769   0.738   0.761   0.811   0.764   0.773   0.832
29    I        -1.066  -1.020  -1.194  -1.170  -1.107  -1.337  -1.189  -1.119  -1.298
30    P         0.717   0.724   0.769   0.777   0.782   0.846   0.764   0.773   0.832
31    V         0.717   0.724   0.769   0.703   0.721   0.781   0.764   0.773   0.832
32    I         0.717   0.724   0.769   0.610   0.610   0.700   0.764   0.773   0.832
33    C         0.717   0.724   0.769   1.037   1.046   1.074   0.764   0.773   0.832
34    E         0.717   0.724   0.769   0.569   0.598   0.664   0.764   0.773   0.832
35    K        -0.029  -0.109  -0.439  -0.139  -0.247  -0.717  -0.040  -0.156  -0.704
36    A        -1.212  -1.251  -1.201  -1.282  -1.312  -1.192  -1.418  -1.443  -1.307
37    E        -0.740  -0.918  -1.180  -0.698  -0.919  -1.289  -0.729  -0.959  -1.279
38    K        -0.396  -0.370  -0.467  -0.578  -0.548  -0.628  -0.614  -0.570  -0.755
39    S         0.717   0.724   0.769   0.755   0.758   0.826   0.764   0.773   0.832
40    D         0.717   0.724   0.769   0.703   0.712   0.781   0.764   0.773   0.832
41    I         0.717   0.724   0.769   0.610   0.610   0.700   0.764   0.773   0.832
42    P        -1.066  -1.068  -1.192  -0.948  -0.963  -1.113  -1.189  -1.192  -1.295
43    E        -2.028  -2.063  -2.130  -1.922  -1.971  -2.032  -1.763  -1.797  -1.750
44    I        -0.029  -0.033  -0.434  -0.043  -0.057  -0.648  -0.040  -0.046  -0.696
45    D         0.717   0.724   0.769   0.703   0.712   0.781   0.764   0.773   0.832
46    K         0.717   0.724   0.769   0.510   0.512   0.612   0.764   0.773   0.832
47    R        -0.593  -0.583  -0.481  -0.908  -0.889  -0.757  -0.959  -0.941  -0.780
48    K         0.717   0.724   0.769   0.510   0.512   0.612   0.764   0.773   0.832
49    Y         0.717   0.724   0.769   0.817   0.835   0.881   0.764   0.773   0.832
50    L         0.717   0.724   0.769   0.777   0.788   0.846   0.764   0.773   0.832
51    V         0.717   0.724   0.769   0.703   0.721   0.781   0.764   0.773   0.832
52    P         0.717   0.724   0.769   0.777   0.782   0.846   0.764   0.773   0.832
53    A        -1.685  -1.763  -1.722  -1.507  -1.627  -1.578  -1.648  -1.728  -1.583
54    D         0.717   0.724   0.769   0.703   0.712   0.781   0.764   0.773   0.832
55    L         0.717   0.724   0.769   0.777   0.788   0.846   0.764   0.773   0.832
56    T        -0.029  -0.016  -0.433   0.204   0.216  -0.436  -0.040  -0.020  -0.694
57    V         0.717   0.724   0.769   0.703   0.721   0.781   0.764   0.773   0.832
58    G         0.717   0.724   0.769   0.890   0.894   0.944   0.764   0.773   0.832
59    Q         0.717   0.724   0.769   0.939   0.949   0.988   0.764   0.773   0.832
60    F         0.717   0.724   0.769   0.709   0.707   0.786   0.764   0.773   0.832
61    V         0.717   0.724   0.769   0.703   0.721   0.781   0.764   0.773   0.832
62    Y         0.717   0.724   0.769   0.817   0.835   0.881   0.764   0.773   0.832
63    V         0.717   0.724   0.769   0.703   0.721   0.781   0.764   0.773   0.832
64    I         0.717   0.724   0.769   0.610   0.610   0.700   0.764   0.773   0.832
65    R         0.717   0.724   0.769   0.738   0.761   0.811   0.764   0.773   0.832
66    K         0.717   0.724   0.769   0.510   0.512   0.612   0.764   0.773   0.832
67    R         0.717   0.724   0.769   0.738   0.761   0.811   0.764   0.773   0.832
68    I         0.717   0.724   0.769   0.610   0.610   0.700   0.764   0.773   0.832
69    M        -0.396  -0.346  -0.443  -0.574  -0.513  -0.597  -0.614  -0.530  -0.712
70    L         0.717   0.724   0.769   0.777   0.788   0.846   0.764   0.773   0.832
71    P        -0.593  -0.583  -0.481  -0.744  -0.741  -0.586  -0.959  -0.941  -0.780
72    P        -1.212  -1.252  -1.201  -1.264  -1.315  -1.210  -1.418  -1.445  -1.307
73    E         0.717   0.724   0.769   0.569   0.598   0.664   0.764   0.773   0.832
74    K        -0.029   0.018  -0.432  -0.139  -0.089  -0.705  -0.040   0.026  -0.692
75    A         0.717   0.724   0.769   0.789   0.804   0.856   0.764   0.773   0.832
76    I         0.717   0.724   0.769   0.610   0.610   0.700   0.764   0.773   0.832
77    F         0.717   0.724   0.769   0.709   0.707   0.786   0.764   0.773   0.832
78    I         0.717   0.724   0.769   0.610   0.610   0.700   0.764   0.773   0.832
79    F         0.717   0.724   0.769   0.709   0.707   0.786   0.764   0.773   0.832
80    V        -0.396  -0.452  -0.476  -0.511  -0.612  -0.710  -0.614  -0.709  -0.771
81    N        -0.593  -0.583  -0.481  -0.599  -0.585  -0.481  -0.959  -0.941  -0.780
82    D        -0.396  -0.408  -0.465  -0.524  -0.545  -0.707  -0.614  -0.634  -0.751
83    T        -1.426  -1.380  -1.213  -1.642  -1.585  -1.316  -1.648  -1.588  -1.321
84    L         0.717   0.724   0.769   0.777   0.788   0.846   0.764   0.773   0.832
85    P         0.717   0.724   0.769   0.777   0.782   0.846   0.764   0.773   0.832
86    P         0.717   0.724   0.769   0.777   0.782   0.846   0.764   0.773   0.832
87    T         0.717   0.724   0.769   0.851   0.856   0.910   0.764   0.773   0.832
88    A         0.717   0.724   0.769   0.789   0.804   0.856   0.764   0.773   0.832
89    A        -0.029  -0.033  -0.434   0.143   0.142  -0.486  -0.040  -0.046  -0.696
90    L         0.717   0.724   0.769   0.777   0.788   0.846   0.764   0.773   0.832
91    M        -0.029   0.005  -0.430   0.317   0.357  -0.394  -0.040   0.008  -0.690
92    S         0.717   0.724   0.769   0.755   0.758   0.826   0.764   0.773   0.832
93    A        -1.769  -1.711  -1.720  -1.628  -1.564  -1.505  -1.763  -1.702  -1.580
94    I        -0.029  -0.033  -0.434  -0.043  -0.057  -0.648  -0.040  -0.046  -0.696
95    Y         0.717   0.724   0.769   0.817   0.835   0.881   0.764   0.773   0.832
96    Q        -0.593  -0.589  -0.477  -0.826  -0.806  -0.614  -0.959  -0.952  -0.772
97    E        -0.029  -0.109  -0.439  -0.049  -0.126  -0.570  -0.040  -0.156  -0.704
98    H         0.717   0.724   0.769   1.010   1.020   1.050   0.764   0.773   0.832
99    K         0.717   0.724   0.769   0.510   0.512   0.612   0.764   0.773   0.832
100   D        -0.029  -0.141  -0.431   0.057  -0.089  -0.546  -0.040  -0.204  -0.691
101   K        -0.593  -0.589  -0.477  -1.063  -1.052  -0.869  -0.959  -0.952  -0.772
102   D         0.717   0.724   0.769   0.703   0.712   0.781   0.764   0.773   0.832
103   G         0.717   0.724   0.769   0.890   0.894   0.944   0.764   0.773   0.832
104   F         0.717   0.724   0.769   0.709   0.707   0.786   0.764   0.773   0.832
105   L         0.717   0.724   0.769   0.777   0.788   0.846   0.764   0.773   0.832
106   Y         0.717   0.724   0.769   0.817   0.835   0.881   0.764   0.773   0.832
107   V        -0.396  -0.408  -0.465  -0.511  -0.532  -0.685  -0.614  -0.634  -0.751
108   T        -1.066  -1.113  -1.197  -0.918  -0.986  -1.158  -1.189  -1.234  -1.301
109   Y         0.717   0.724   0.769   0.817   0.835   0.881   0.764   0.773   0.832
110   S         0.717   0.724   0.769   0.755   0.758   0.826   0.764   0.773   0.832
111   G        -0.029  -0.109  -0.439   0.243   0.135  -0.418  -0.040  -0.156  -0.704
112   E         0.717   0.724   0.769   0.569   0.598   0.664   0.764   0.773   0.832
113   N         0.717   0.724   0.769   0.972   0.986   1.017   0.764   0.773   0.832
114   T         0.717   0.724   0.769   0.851   0.856   0.910   0.764   0.773   0.832
115   F         0.717   0.724   0.769   0.709   0.707   0.786   0.764   0.773   0.832
116   G         0.717   0.724   0.769   0.890   0.894   0.944   0.764   0.773   0.832
117   -        -1.286  -1.304  -1.207  -1.512  -1.507  -1.349  -1.482  -1.489  -1.314
118   -        -2.832  -2.777  -2.475  -2.824  -2.761  -2.305  -2.299  -2.248  -1.870
119   -        -1.744  -1.850  -1.727  -1.817  -1.963  -1.763  -1.686  -1.803  -1.586
120   -        -2.832  -2.777  -2.475  -2.393  -2.362  -1.928  -2.299  -2.248  -1.870
121   -        -1.921  -1.780  -1.727  -2.094  -1.869  -1.819  -1.882  -1.719  -1.586
122   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
123   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
124   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
125   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
126   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
127   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
128   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
129   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
130   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
131   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
132   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
133   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
134   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
135   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
136   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
137   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
138   -        -1.921  -1.914  -1.732  -1.987  -1.987  -1.751  -1.882  -1.866  -1.591
139   R        -2.745  -2.681  -2.467  -2.568  -2.486  -2.134  -2.237  -2.175  -1.864
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P38182
Output conservation file - output/P38182
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
