1     M         1.136   1.136   1.136   1.417   1.417   1.417   1.136   1.136   1.136
2     E        -0.876  -0.876  -0.876  -1.005  -1.005  -1.005  -0.876  -0.876  -0.876
3     A        -0.876  -0.876  -0.876  -0.907  -0.907  -0.907  -0.876  -0.876  -0.876
4     T         1.136   1.136   1.136   1.111   1.111   1.111   1.136   1.136   1.136
5     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
6     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
7     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
8     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
9     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
10    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
11    I        -0.876  -0.876  -0.876  -1.005  -1.005  -1.005  -0.876  -0.876  -0.876
12    K         1.136   1.136   1.136   1.062   1.062   1.062   1.136   1.136   1.136
13    V         1.136   1.136   1.136   0.928   0.928   0.928   1.136   1.136   1.136
14    V         1.136   1.136   1.136   0.928   0.928   0.928   1.136   1.136   1.136
15    L         1.136   1.136   1.136   0.978   0.978   0.978   1.136   1.136   1.136
16    L         1.136   1.136   1.136   0.978   0.978   0.978   1.136   1.136   1.136
17    G         1.136   1.136   1.136   1.128   1.128   1.128   1.136   1.136   1.136
18    D        -0.876  -0.876  -0.876  -0.846  -0.846  -0.846  -0.876  -0.876  -0.876
19    S         1.136   1.136   1.136   0.978   0.978   0.978   1.136   1.136   1.136
20    S        -0.876  -0.876  -0.876  -0.968  -0.968  -0.968  -0.876  -0.876  -0.876
21    V         1.136   1.136   1.136   0.928   0.928   0.928   1.136   1.136   1.136
22    G         1.136   1.136   1.136   1.128   1.128   1.128   1.136   1.136   1.136
23    K         1.136   1.136   1.136   1.062   1.062   1.062   1.136   1.136   1.136
24    T        -0.876  -0.876  -0.876  -0.968  -0.968  -0.968  -0.876  -0.876  -0.876
25    S        -0.876  -0.876  -0.876  -0.968  -0.968  -0.968  -0.876  -0.876  -0.876
26    I         1.136   1.136   1.136   1.062   1.062   1.062   1.136   1.136   1.136
27    V         1.136   1.136   1.136   0.928   0.928   0.928   1.136   1.136   1.136
28    T         1.136   1.136   1.136   1.111   1.111   1.111   1.136   1.136   1.136
29    R         1.136   1.136   1.136   1.258   1.258   1.258   1.136   1.136   1.136
30    L        -0.876  -0.876  -0.876  -0.787  -0.787  -0.787  -0.876  -0.876  -0.876
31    K        -0.876  -0.876  -0.876  -1.005  -1.005  -1.005  -0.876  -0.876  -0.876
32    S        -0.876  -0.876  -0.876  -0.968  -0.968  -0.968  -0.876  -0.876  -0.876
33    G         1.136   1.136   1.136   1.128   1.128   1.128   1.136   1.136   1.136
34    K         1.136   1.136   1.136   1.062   1.062   1.062   1.136   1.136   1.136
35    F        -0.876  -0.876  -0.876  -0.567  -0.567  -0.567  -0.876  -0.876  -0.876
36    L         1.136   1.136   1.136   0.978   0.978   0.978   1.136   1.136   1.136
37    A        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
38    K        -0.876  -0.876  -0.876  -0.798  -0.798  -0.798  -0.876  -0.876  -0.876
39    H        -0.876  -0.876  -0.876  -0.579  -0.579  -0.579  -0.876  -0.876  -0.876
40    A        -0.876  -0.876  -0.876  -0.822  -0.822  -0.822  -0.876  -0.876  -0.876
41    A         1.136   1.136   1.136   1.111   1.111   1.111   1.136   1.136   1.136
42    T         1.136   1.136   1.136   1.111   1.111   1.111   1.136   1.136   1.136
43    I         1.136   1.136   1.136   1.062   1.062   1.062   1.136   1.136   1.136
44    G         1.136   1.136   1.136   1.128   1.128   1.128   1.136   1.136   1.136
45    A         1.136   1.136   1.136   1.111   1.111   1.111   1.136   1.136   1.136
46    A         1.136   1.136   1.136   1.111   1.111   1.111   1.136   1.136   1.136
47    F        -0.876  -0.876  -0.876  -0.567  -0.567  -0.567  -0.876  -0.876  -0.876
48    I        -0.876  -0.876  -0.876  -1.043  -1.043  -1.043  -0.876  -0.876  -0.876
49    T         1.136   1.136   1.136   1.111   1.111   1.111   1.136   1.136   1.136
50    K         1.136   1.136   1.136   1.062   1.062   1.062   1.136   1.136   1.136
51    T        -0.876  -0.876  -0.876  -0.810  -0.810  -0.810  -0.876  -0.876  -0.876
52    I        -0.876  -0.876  -0.876  -1.005  -1.005  -1.005  -0.876  -0.876  -0.876
53    E         1.136   1.136   1.136   1.062   1.062   1.062   1.136   1.136   1.136
54    V        -0.876  -0.876  -0.876  -0.870  -0.870  -0.870  -0.876  -0.876  -0.876
55    P        -0.876  -0.876  -0.876  -0.705  -0.705  -0.705  -0.876  -0.876  -0.876
56    S        -0.876  -0.876  -0.876  -0.858  -0.858  -0.858  -0.876  -0.876  -0.876
57    N        -0.876  -0.876  -0.876  -0.763  -0.763  -0.763  -0.876  -0.876  -0.876
58    D        -0.876  -0.876  -0.876  -0.798  -0.798  -0.798  -0.876  -0.876  -0.876
59    S        -0.876  -0.876  -0.876  -0.907  -0.907  -0.907  -0.876  -0.876  -0.876
60    S        -0.876  -0.876  -0.876  -1.106  -1.106  -1.106  -0.876  -0.876  -0.876
61    T        -0.876  -0.876  -0.876  -0.740  -0.740  -0.740  -0.876  -0.876  -0.876
62    E        -0.876  -0.876  -0.876  -0.810  -0.810  -0.810  -0.876  -0.876  -0.876
63    K        -0.876  -0.876  -0.876  -1.043  -1.043  -1.043  -0.876  -0.876  -0.876
64    R         1.136   1.136   1.136   1.258   1.258   1.258   1.136   1.136   1.136
65    I        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
66    H        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
67    M        -0.876  -0.876  -0.876  -0.740  -0.740  -0.740  -0.876  -0.876  -0.876
68    E         1.136   1.136   1.136   1.062   1.062   1.062   1.136   1.136   1.136
69    I         1.136   1.136   1.136   1.062   1.062   1.062   1.136   1.136   1.136
70    W         1.136   1.136   1.136   1.463   1.463   1.463   1.136   1.136   1.136
71    D         1.136   1.136   1.136   1.193   1.193   1.193   1.136   1.136   1.136
72    T         1.136   1.136   1.136   1.111   1.111   1.111   1.136   1.136   1.136
73    A         1.136   1.136   1.136   1.111   1.111   1.111   1.136   1.136   1.136
74    G         1.136   1.136   1.136   1.128   1.128   1.128   1.136   1.136   1.136
75    Q         1.136   1.136   1.136   1.242   1.242   1.242   1.136   1.136   1.136
76    E         1.136   1.136   1.136   1.062   1.062   1.062   1.136   1.136   1.136
77    R         1.136   1.136   1.136   1.258   1.258   1.258   1.136   1.136   1.136
78    Y         1.136   1.136   1.136   1.290   1.290   1.290   1.136   1.136   1.136
79    K        -0.876  -0.876  -0.876  -0.798  -0.798  -0.798  -0.876  -0.876  -0.876
80    S         1.136   1.136   1.136   0.978   0.978   0.978   1.136   1.136   1.136
81    L         1.136   1.136   1.136   0.978   0.978   0.978   1.136   1.136   1.136
82    V        -0.876  -0.876  -0.876  -1.005  -1.005  -1.005  -0.876  -0.876  -0.876
83    P         1.136   1.136   1.136   1.385   1.385   1.385   1.136   1.136   1.136
84    M         1.136   1.136   1.136   1.417   1.417   1.417   1.136   1.136   1.136
85    Y         1.136   1.136   1.136   1.290   1.290   1.290   1.136   1.136   1.136
86    Y         1.136   1.136   1.136   1.290   1.290   1.290   1.136   1.136   1.136
87    R         1.136   1.136   1.136   1.258   1.258   1.258   1.136   1.136   1.136
88    D        -0.876  -0.876  -0.876  -0.763  -0.763  -0.763  -0.876  -0.876  -0.876
89    A        -0.876  -0.876  -0.876  -0.870  -0.870  -0.870  -0.876  -0.876  -0.876
90    N        -0.876  -0.876  -0.876  -0.763  -0.763  -0.763  -0.876  -0.876  -0.876
91    I        -0.876  -0.876  -0.876  -1.043  -1.043  -1.043  -0.876  -0.876  -0.876
92    A         1.136   1.136   1.136   1.111   1.111   1.111   1.136   1.136   1.136
93    L        -0.876  -0.876  -0.876  -1.005  -1.005  -1.005  -0.876  -0.876  -0.876
94    I        -0.876  -0.876  -0.876  -1.043  -1.043  -1.043  -0.876  -0.876  -0.876
95    V         1.136   1.136   1.136   0.928   0.928   0.928   1.136   1.136   1.136
96    F         1.136   1.136   1.136   1.354   1.354   1.354   1.136   1.136   1.136
97    E        -0.876  -0.876  -0.876  -0.846  -0.846  -0.846  -0.876  -0.876  -0.876
98    L        -0.876  -0.876  -0.876  -1.106  -1.106  -1.106  -0.876  -0.876  -0.876
99    G        -0.876  -0.876  -0.876  -0.956  -0.956  -0.956  -0.876  -0.876  -0.876
100   D        -0.876  -0.876  -0.876  -0.763  -0.763  -0.763  -0.876  -0.876  -0.876
101   V        -0.876  -0.876  -0.876  -1.106  -1.106  -1.106  -0.876  -0.876  -0.876
102   S        -0.876  -0.876  -0.876  -0.858  -0.858  -0.858  -0.876  -0.876  -0.876
103   S         1.136   1.136   1.136   0.978   0.978   0.978   1.136   1.136   1.136
104   L         1.136   1.136   1.136   0.978   0.978   0.978   1.136   1.136   1.136
105   Q        -0.876  -0.876  -0.876  -0.870  -0.870  -0.870  -0.876  -0.876  -0.876
106   C        -0.876  -0.876  -0.876  -0.434  -0.434  -0.434  -0.876  -0.876  -0.876
107   A         1.136   1.136   1.136   1.111   1.111   1.111   1.136   1.136   1.136
108   K        -0.876  -0.876  -0.876  -0.658  -0.658  -0.658  -0.876  -0.876  -0.876
109   T        -0.876  -0.876  -0.876  -0.907  -0.907  -0.907  -0.876  -0.876  -0.876
110   W         1.136   1.136   1.136   1.463   1.463   1.463   1.136   1.136   1.136
111   F        -0.876  -0.876  -0.876  -0.728  -0.728  -0.728  -0.876  -0.876  -0.876
112   Q        -0.876  -0.876  -0.876  -0.716  -0.716  -0.716  -0.876  -0.876  -0.876
113   D        -0.876  -0.876  -0.876  -0.846  -0.846  -0.846  -0.876  -0.876  -0.876
114   L         1.136   1.136   1.136   0.978   0.978   0.978   1.136   1.136   1.136
115   Q        -0.876  -0.876  -0.876  -0.728  -0.728  -0.728  -0.876  -0.876  -0.876
116   D        -0.876  -0.876  -0.876  -0.810  -0.810  -0.810  -0.876  -0.876  -0.876
117   R        -0.876  -0.876  -0.876  -0.635  -0.635  -0.635  -0.876  -0.876  -0.876
118   A        -0.876  -0.876  -0.876  -1.005  -1.005  -1.005  -0.876  -0.876  -0.876
119   Q        -0.876  -0.876  -0.876  -0.810  -0.810  -0.810  -0.876  -0.876  -0.876
120   G        -0.876  -0.876  -0.876  -0.810  -0.810  -0.810  -0.876  -0.876  -0.876
121   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
122   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
123   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
124   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
125   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
126   T        -0.876  -0.876  -0.876  -0.907  -0.907  -0.907  -0.876  -0.876  -0.876
127   Q        -0.876  -0.876  -0.876  -0.728  -0.728  -0.728  -0.876  -0.876  -0.876
128   V        -0.876  -0.876  -0.876  -1.043  -1.043  -1.043  -0.876  -0.876  -0.876
129   I        -0.876  -0.876  -0.876  -1.043  -1.043  -1.043  -0.876  -0.876  -0.876
130   I        -0.876  -0.876  -0.876  -1.005  -1.005  -1.005  -0.876  -0.876  -0.876
131   V         1.136   1.136   1.136   0.928   0.928   0.928   1.136   1.136   1.136
132   G         1.136   1.136   1.136   1.128   1.128   1.128   1.136   1.136   1.136
133   N         1.136   1.136   1.136   1.177   1.177   1.177   1.136   1.136   1.136
134   K         1.136   1.136   1.136   1.062   1.062   1.062   1.136   1.136   1.136
135   Y        -0.876  -0.876  -0.876  -0.522  -0.522  -0.522  -0.876  -0.876  -0.876
136   D         1.136   1.136   1.136   1.193   1.193   1.193   1.136   1.136   1.136
137   L         1.136   1.136   1.136   0.978   0.978   0.978   1.136   1.136   1.136
138   V        -0.876  -0.876  -0.876  -0.775  -0.775  -0.775  -0.876  -0.876  -0.876
139   C        -0.876  -0.876  -0.876  -0.740  -0.740  -0.740  -0.876  -0.876  -0.876
140   E        -0.876  -0.876  -0.876  -0.846  -0.846  -0.846  -0.876  -0.876  -0.876
141   E         1.136   1.136   1.136   1.062   1.062   1.062   1.136   1.136   1.136
142   H        -0.876  -0.876  -0.876  -0.658  -0.658  -0.658  -0.876  -0.876  -0.876
143   S        -0.876  -0.876  -0.876  -0.858  -0.858  -0.858  -0.876  -0.876  -0.876
144   G        -0.876  -0.876  -0.876  -0.956  -0.956  -0.956  -0.876  -0.876  -0.876
145   E        -0.876  -0.876  -0.876  -0.907  -0.907  -0.907  -0.876  -0.876  -0.876
146   V        -0.876  -0.876  -0.876  -1.106  -1.106  -1.106  -0.876  -0.876  -0.876
147   T        -0.876  -0.876  -0.876  -0.670  -0.670  -0.670  -0.876  -0.876  -0.876
148   I        -0.876  -0.876  -0.876  -0.810  -0.810  -0.810  -0.876  -0.876  -0.876
149   P        -0.876  -0.876  -0.876  -0.567  -0.567  -0.567  -0.876  -0.876  -0.876
150   A        -0.876  -0.876  -0.876  -1.005  -1.005  -1.005  -0.876  -0.876  -0.876
151   E         1.136   1.136   1.136   1.062   1.062   1.062   1.136   1.136   1.136
152   L        -0.876  -0.876  -0.876  -1.005  -1.005  -1.005  -0.876  -0.876  -0.876
153   Q         1.136   1.136   1.136   1.242   1.242   1.242   1.136   1.136   1.136
154   G        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
155   L        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
156   P        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
157   Y        -0.876  -0.876  -0.876  -0.567  -0.567  -0.567  -0.876  -0.876  -0.876
158   V        -0.876  -0.876  -0.876  -0.907  -0.907  -0.907  -0.876  -0.876  -0.876
159   A         1.136   1.136   1.136   1.111   1.111   1.111   1.136   1.136   1.136
160   V         1.136   1.136   1.136   0.928   0.928   0.928   1.136   1.136   1.136
161   S         1.136   1.136   1.136   0.978   0.978   0.978   1.136   1.136   1.136
162   A         1.136   1.136   1.136   1.111   1.111   1.111   1.136   1.136   1.136
163   K         1.136   1.136   1.136   1.062   1.062   1.062   1.136   1.136   1.136
164   T        -0.876  -0.876  -0.876  -0.968  -0.968  -0.968  -0.876  -0.876  -0.876
165   G         1.136   1.136   1.136   1.128   1.128   1.128   1.136   1.136   1.136
166   Y        -0.876  -0.876  -0.876  -0.775  -0.775  -0.775  -0.876  -0.876  -0.876
167   N        -0.876  -0.876  -0.876  -0.810  -0.810  -0.810  -0.876  -0.876  -0.876
168   F        -0.876  -0.876  -0.876  -0.728  -0.728  -0.728  -0.876  -0.876  -0.876
169   D        -0.876  -0.876  -0.876  -0.846  -0.846  -0.846  -0.876  -0.876  -0.876
170   T        -0.876  -0.876  -0.876  -0.907  -0.907  -0.907  -0.876  -0.876  -0.876
171   L         1.136   1.136   1.136   0.978   0.978   0.978   1.136   1.136   1.136
172   N        -0.876  -0.876  -0.876  -0.647  -0.647  -0.647  -0.876  -0.876  -0.876
173   K        -0.876  -0.876  -0.876  -0.846  -0.846  -0.846  -0.876  -0.876  -0.876
174   I        -0.876  -0.876  -0.876  -0.658  -0.658  -0.658  -0.876  -0.876  -0.876
175   I         1.136   1.136   1.136   1.062   1.062   1.062   1.136   1.136   1.136
176   I        -0.876  -0.876  -0.876  -1.043  -1.043  -1.043  -0.876  -0.876  -0.876
177   S        -0.876  -0.876  -0.876  -1.005  -1.005  -1.005  -0.876  -0.876  -0.876
178   L        -0.876  -0.876  -0.876  -0.956  -0.956  -0.956  -0.876  -0.876  -0.876
179   V        -0.876  -0.876  -0.876  -1.043  -1.043  -1.043  -0.876  -0.876  -0.876
180   P         1.136   1.136   1.136   1.385   1.385   1.385   1.136   1.136   1.136
181   E        -0.876  -0.876  -0.876  -1.005  -1.005  -1.005  -0.876  -0.876  -0.876
182   S        -0.876  -0.876  -0.876  -0.907  -0.907  -0.907  -0.876  -0.876  -0.876
183   Q         1.136   1.136   1.136   1.242   1.242   1.242   1.136   1.136   1.136
184   F         1.136   1.136   1.136   1.354   1.354   1.354   1.136   1.136   1.136
185   K        -0.876  -0.876  -0.876  -0.907  -0.907  -0.907  -0.876  -0.876  -0.876
186   T        -0.876  -0.876  -0.876  -0.968  -0.968  -0.968  -0.876  -0.876  -0.876
187   L        -0.876  -0.876  -0.876  -1.005  -1.005  -1.005  -0.876  -0.876  -0.876
188   S         1.136   1.136   1.136   0.978   0.978   0.978   1.136   1.136   1.136
189   K        -0.876  -0.876  -0.876  -0.907  -0.907  -0.907  -0.876  -0.876  -0.876
190   N        -0.876  -0.876  -0.876  -0.822  -0.822  -0.822  -0.876  -0.876  -0.876
191   N        -0.876  -0.876  -0.876  -0.956  -0.956  -0.956  -0.876  -0.876  -0.876
192   E         1.136   1.136   1.136   1.062   1.062   1.062   1.136   1.136   1.136
193   Q         1.136   1.136   1.136   1.242   1.242   1.242   1.136   1.136   1.136
194   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
195   G        -0.876  -0.876  -0.876  -0.798  -0.798  -0.798  -0.876  -0.876  -0.876
196   N         1.136   1.136   1.136   1.177   1.177   1.177   1.136   1.136   1.136
197   I        -0.876  -0.876  -0.876  -1.043  -1.043  -1.043  -0.876  -0.876  -0.876
198   L        -0.876  -0.876  -0.876  -1.106  -1.106  -1.106  -0.876  -0.876  -0.876
199   E        -0.876  -0.876  -0.876  -0.858  -0.858  -0.858  -0.876  -0.876  -0.876
200   I        -0.876  -0.876  -0.876  -1.005  -1.005  -1.005  -0.876  -0.876  -0.876
201   N         1.136   1.136   1.136   1.177   1.177   1.177   1.136   1.136   1.136
202   K        -0.876  -0.876  -0.876  -0.858  -0.858  -0.858  -0.876  -0.876  -0.876
203   K         1.136   1.136   1.136   1.062   1.062   1.062   1.136   1.136   1.136
204   K        -0.876  -0.876  -0.876  -1.005  -1.005  -1.005  -0.876  -0.876  -0.876
205   S        -0.876  -0.876  -0.876  -1.106  -1.106  -1.106  -0.876  -0.876  -0.876
206   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
207   G        -0.876  -0.876  -0.876  -0.763  -0.763  -0.763  -0.876  -0.876  -0.876
208   S         1.136   1.136   1.136   0.978   0.978   0.978   1.136   1.136   1.136
209   G        -0.876  -0.876  -0.876  -0.858  -0.858  -0.858  -0.876  -0.876  -0.876
210   C         1.136   1.136   1.136   1.417   1.417   1.417   1.136   1.136   1.136
211   I        -0.876  -0.876  -0.876  -0.858  -0.858  -0.858  -0.876  -0.876  -0.876
212   C         1.136   1.136   1.136   1.417   1.417   1.417   1.136   1.136   1.136
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P38146
Output conservation file - output/P38146
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
