1     M         1.499   1.532   1.621   1.824   1.868   2.004   1.663   1.715   1.864
2     V         0.739   0.629   0.521   1.010   0.876   0.702   0.762   0.579   0.357
3     T         0.233   0.250   0.362   0.121   0.123   0.297  -0.140  -0.119   0.050
4     F         1.499   1.532   1.621   1.759   1.802   1.939   1.663   1.715   1.864
5     N         0.214   0.244   0.349   0.142   0.174   0.328  -0.177  -0.131   0.024
6     C         0.739   0.629   0.521   0.933   0.752   0.472   0.762   0.579   0.357
7     E         0.739   0.629   0.521   0.879   0.736   0.579   0.762   0.579   0.357
8     V         1.499   1.532   1.621   1.647   1.699   1.827   1.663   1.715   1.864
9     C         1.499   1.532   1.621   1.684   1.729   1.864   1.663   1.715   1.864
10    N         0.739   0.629   0.521   0.961   0.823   0.651   0.762   0.579   0.357
11    D         1.499   1.532   1.621   1.591   1.629   1.772   1.663   1.715   1.864
12    T         0.739   0.629   0.521   0.918   0.773   0.629   0.762   0.579   0.357
13    V         0.390   0.317   0.362   0.543   0.422   0.455   0.161   0.013   0.050
14    P        -1.236  -1.314  -1.292  -0.829  -1.036  -1.036  -1.041  -1.116  -1.045
15    K         1.499   1.532   1.621   0.836   0.885   1.020   1.663   1.715   1.864
16    K         0.739   0.629   0.521   0.227   0.110   0.000   0.762   0.579   0.357
17    N         0.233   0.296   0.366  -0.421  -0.365  -0.233  -0.140  -0.030   0.059
18    T         0.233   0.296   0.366   0.168   0.255   0.353  -0.140  -0.030   0.059
19    E        -0.363  -0.381  -0.341  -0.325  -0.346  -0.287  -0.590  -0.597  -0.522
20    K        -0.363  -0.381  -0.341  -0.832  -0.776  -0.740  -0.590  -0.597  -0.522
21    H         1.499   1.532   1.621   1.775   1.817   1.955   1.663   1.715   1.864
22    Y        -1.236  -1.314  -1.292  -0.668  -0.769  -0.751  -1.041  -1.116  -1.045
23    Y        -0.501  -0.506  -0.389  -0.416  -0.445  -0.292  -0.741  -0.746  -0.582
24    R         0.739   0.693   0.527   1.129   1.067   0.821   0.762   0.685   0.369
25    C         1.499   1.532   1.621   1.684   1.729   1.864   1.663   1.715   1.864
26    P        -0.868  -0.915  -0.880  -0.315  -0.379  -0.315  -0.891  -0.938  -0.854
27    N        -0.273  -0.161  -0.299  -0.003   0.170  -0.057  -0.440  -0.270  -0.457
28    A         1.499   1.532   1.621   1.739   1.781   1.919   1.663   1.715   1.864
29    Y         1.499   1.532   1.621   1.656   1.706   1.837   1.663   1.715   1.864
30    Y         0.233   0.296   0.366   0.374   0.481   0.558  -0.140  -0.030   0.059
31    T         0.739   0.814   0.545   0.898   0.980   0.623   0.762   0.880   0.404
32    C         1.499   1.532   1.621   1.684   1.729   1.864   1.663   1.715   1.864
33    I         1.499   1.532   1.621   1.651   1.695   1.832   1.663   1.715   1.864
34    D         1.499   1.532   1.621   1.591   1.629   1.772   1.663   1.715   1.864
35    C         1.499   1.532   1.621   1.684   1.729   1.864   1.663   1.715   1.864
36    S         0.390   0.357   0.369   0.413   0.344   0.343   0.161   0.089   0.064
37    K        -0.868  -0.756  -0.854  -1.047  -0.801  -0.996  -0.891  -0.746  -0.824
38    T         1.499   1.532   1.621   1.544   1.577   1.725   1.663   1.715   1.864
39    F         1.499   1.532   1.621   1.761   1.806   1.941   1.663   1.715   1.864
40    E        -1.374  -1.368  -1.289  -1.007  -0.997  -0.885  -1.191  -1.174  -1.045
41    D        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
42    G         0.023  -0.139  -0.266   0.475   0.269   0.079   0.011  -0.219  -0.406
43    V        -0.868  -0.837  -0.863  -0.594  -0.553  -0.576  -0.891  -0.837  -0.834
44    S        -0.363  -0.299  -0.335  -0.319  -0.237  -0.273  -0.590  -0.506  -0.514
45    Y         0.739   0.693   0.527   1.050   1.005   0.789   0.762   0.685   0.369
46    K        -0.363  -0.381  -0.341  -0.631  -0.567  -0.522  -0.590  -0.597  -0.522
47    N        -0.501  -0.467  -0.387  -1.020  -0.979  -0.804  -0.741  -0.693  -0.579
48    H         1.499   1.532   1.621   1.775   1.817   1.955   1.663   1.715   1.864
49    T         0.739   0.795   0.547   0.918   0.978   0.669   0.762   0.850   0.406
50    S         0.390   0.477   0.432   0.403   0.525   0.437   0.161   0.312   0.189
51    C         1.499   1.532   1.621   1.684   1.729   1.864   1.663   1.715   1.864
52    I         0.739   0.629   0.521   1.053   0.928   0.794   0.762   0.579   0.357
53    S         0.390   0.357   0.369   0.392   0.314   0.308   0.161   0.089   0.064
54    E         1.499   1.532   1.621   1.515   1.557   1.696   1.663   1.715   1.864
55    D         0.233   0.296   0.366   0.317   0.421   0.501  -0.140  -0.030   0.059
56    E         0.739   0.629   0.521   0.876   0.736   0.572   0.762   0.579   0.357
57    K         0.739   0.629   0.521   0.232   0.115   0.011   0.762   0.579   0.357
58    Y         1.499   1.532   1.621   1.656   1.706   1.837   1.663   1.715   1.864
59    Q         0.233   0.250   0.362   0.135   0.154   0.311  -0.140  -0.119   0.050
60    K         0.739   0.693   0.527   0.216   0.173  -0.016   0.762   0.685   0.369
61    A         0.233   0.269   0.355   0.372   0.421   0.537  -0.140  -0.081   0.036
62    L         1.499   1.532   1.621   1.591   1.623   1.772   1.663   1.715   1.864
63    Y         0.739   0.693   0.527   1.047   1.003   0.784   0.762   0.685   0.369
64    K         0.390   0.317   0.362  -0.174  -0.243  -0.102   0.161   0.013   0.050
65    G         0.739   0.629   0.521   1.086   0.956   0.807   0.762   0.579   0.357
66    N         0.023  -0.016  -0.237  -0.438  -0.491  -0.749   0.011  -0.051  -0.363
67    K         0.739   0.763   0.572   0.196   0.235  -0.000   0.762   0.799   0.453
68    K        -0.273  -0.263  -0.297  -0.810  -0.796  -0.799  -0.440  -0.407  -0.456
69    Q         0.023  -0.052  -0.241   0.323   0.220  -0.051   0.011  -0.098  -0.370
70    K        -0.971  -1.000  -0.906  -1.052  -1.067  -1.049  -0.987  -1.018  -0.879
71    Q        -0.262  -0.394  -0.342  -0.264  -0.534  -0.516  -0.398  -0.587  -0.516
72    K         0.252   0.327   0.395  -0.415  -0.319  -0.218  -0.104   0.032   0.116
73    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
74    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
75    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
76    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
77    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
78    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
79    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
80    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
81    Q        -0.262  -0.309  -0.308  -0.097  -0.172  -0.196  -0.398  -0.470  -0.466
82    Q        -0.971  -0.992  -0.901  -0.790  -0.843  -0.717  -0.987  -1.010  -0.874
83    Q         0.023  -0.052  -0.241   0.179   0.032  -0.339   0.011  -0.098  -0.370
84    K        -1.741  -1.683  -1.648  -1.494  -1.384  -1.414  -1.342  -1.286  -1.190
85    Q         0.023  -0.052  -0.241   0.177   0.082  -0.343   0.011  -0.098  -0.370
86    Q        -1.374  -1.350  -1.295  -0.961  -0.933  -0.834  -1.191  -1.160  -1.050
87    Q         0.319   0.434   0.420   0.467   0.657   0.591   0.027   0.233   0.164
88    K         0.319   0.304   0.357  -0.335  -0.346  -0.224   0.027  -0.013   0.040
89    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
90    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
91    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
92    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
93    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
94    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
95    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
96    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
97    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
98    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
99    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
100   Q        -0.966  -0.958  -0.880  -0.903  -0.866  -0.853  -0.995  -0.983  -0.854
101   H        -0.966  -0.963  -0.891  -0.755  -0.769  -0.639  -0.995  -0.984  -0.865
102   Q        -1.823  -1.791  -1.669  -1.751  -1.638  -1.558  -1.404  -1.370  -1.206
103   H        -0.376  -0.318  -0.328  -0.843  -0.775  -0.692  -0.586  -0.519  -0.506
104   Q        -1.394  -1.375  -1.298  -1.272  -1.229  -1.183  -1.200  -1.175  -1.051
105   P        -0.966  -0.948  -0.875  -0.723  -0.713  -0.579  -0.995  -0.970  -0.849
106   V         0.319   0.292   0.369   0.478   0.435   0.532   0.027  -0.037   0.065
107   A        -0.966  -0.936  -0.874  -1.193  -1.120  -1.025  -0.995  -0.959  -0.848
108   T        -1.394  -1.375  -1.298  -1.323  -1.277  -1.227  -1.200  -1.175  -1.051
109   P        -0.456  -0.454  -0.355  -0.325  -0.313  -0.181  -0.693  -0.682  -0.539
110   A        -0.804  -0.793  -0.831  -1.202  -1.197  -1.169  -0.791  -0.779  -0.795
111   K         0.664   0.614   0.522   0.102   0.044  -0.077   0.641   0.553   0.360
112   K        -0.804  -0.800  -0.827  -1.106  -1.084  -1.042  -0.791  -0.788  -0.790
113   V        -1.394  -1.308  -1.297  -1.304  -1.245  -1.212  -1.200  -1.109  -1.050
114   E        -0.109  -0.191  -0.263  -0.106  -0.235  -0.462  -0.177  -0.297  -0.401
115   K         0.664   0.556   0.517   0.102  -0.034  -0.084   0.641   0.452   0.350
116   P        -0.376  -0.375  -0.325  -0.440  -0.477  -0.453  -0.586  -0.568  -0.496
117   V        -1.394  -1.411  -1.314  -1.015  -1.055  -0.937  -1.200  -1.210  -1.065
118   I        -0.804  -0.872  -0.857  -1.142  -1.209  -1.097  -0.791  -0.872  -0.824
119   K         1.499   1.532   1.621   0.838   0.889   1.022   1.663   1.715   1.864
120   -         0.457   0.473   0.460   0.517   0.534   0.480   0.283   0.305   0.241
121   -        -0.428  -0.439  -0.359  -0.630  -0.678  -0.593  -0.637  -0.652  -0.540
122   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
123   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
124   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
125   K        -0.273  -0.332  -0.323  -0.844  -0.904  -0.823  -0.440  -0.518  -0.499
126   A        -1.741  -1.742  -1.643  -1.682  -1.645  -1.450  -1.342  -1.334  -1.187
127   E        -0.868  -0.805  -0.829  -1.324  -1.269  -1.226  -0.891  -0.804  -0.799
128   K         0.739   0.693   0.527   0.216   0.173  -0.016   0.762   0.685   0.369
129   V        -0.966  -0.919  -0.875  -1.228  -1.124  -1.038  -0.995  -0.938  -0.849
130   E        -0.376  -0.316  -0.336  -0.945  -0.884  -0.836  -0.586  -0.495  -0.513
131   K         0.214   0.237   0.355  -0.440  -0.429  -0.282  -0.177  -0.145   0.035
132   T        -0.804  -0.865  -0.859  -0.794  -0.907  -1.018  -0.791  -0.859  -0.827
133   S        -0.804  -0.812  -0.827  -0.509  -0.535  -0.575  -0.791  -0.796  -0.791
134   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
135   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
136   N        -0.109   0.021  -0.221  -0.064   0.120  -0.334  -0.177   0.011  -0.340
137   G        -1.394  -1.261  -1.288  -1.098  -0.865  -0.999  -1.200  -1.058  -1.042
138   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
139   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
140   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
141   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
142   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
143   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
144   I        -1.236  -1.103  -1.237  -0.751  -0.597  -0.761  -1.041  -0.893  -0.994
145   E        -1.374  -1.364  -1.299  -1.065  -1.068  -0.983  -1.191  -1.173  -1.054
146   L        -1.741  -1.750  -1.651  -1.173  -1.204  -1.057  -1.342  -1.340  -1.193
147   H        -1.741  -1.683  -1.636  -1.455  -1.345  -1.352  -1.342  -1.283  -1.181
148   K         0.023  -0.080  -0.213  -0.406  -0.526  -0.662   0.011  -0.136  -0.328
149   G        -1.236  -1.314  -1.292  -0.706  -0.832  -0.827  -1.041  -1.116  -1.045
150   K        -1.741  -1.742  -1.643  -1.589  -1.553  -1.501  -1.342  -1.334  -1.187
151   S         0.390   0.458   0.429   0.413   0.504   0.445   0.161   0.277   0.182
152   L         0.739   0.693   0.527   0.994   0.934   0.747   0.762   0.685   0.369
153   Y         1.499   1.532   1.621   1.656   1.706   1.837   1.663   1.715   1.864
154   K         1.499   1.532   1.621   0.836   0.885   1.020   1.663   1.715   1.864
155   I         0.023  -0.080  -0.213   0.405   0.265   0.071   0.011  -0.136  -0.328
156   L         0.023   0.022  -0.232   0.358   0.346   0.012   0.011   0.009  -0.356
157   K         1.499   1.532   1.621   0.836   0.885   1.020   1.663   1.715   1.864
158   T        -0.868  -0.823  -0.863  -1.313  -1.281  -1.236  -0.891  -0.827  -0.835
159   M        -1.374  -1.361  -1.284  -0.814  -0.811  -0.683  -1.191  -1.170  -1.041
160   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
161   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
162   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
163   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
164   -         0.457   0.459   0.429   0.196   0.184   0.010   0.283   0.279   0.182
165   -         0.457   0.533   0.440  -0.048   0.048  -0.036   0.283   0.412   0.203
166   K        -1.007  -1.034  -0.896  -1.271  -1.240  -1.069  -1.041  -1.052  -0.871
167   D        -0.273  -0.243  -0.313  -0.424  -0.405  -0.589  -0.440  -0.403  -0.483
168   K         0.390   0.344   0.398  -0.288  -0.355  -0.264   0.161   0.064   0.122
169   G        -1.374  -1.405  -1.313  -1.452  -1.491  -1.284  -1.191  -1.208  -1.065
170   A        -0.640  -0.628  -0.784  -0.404  -0.409  -0.709  -0.590  -0.572  -0.740
171   K        -0.640  -0.780  -0.820  -1.059  -1.243  -1.245  -0.590  -0.757  -0.782
172   K         1.499   1.532   1.621   0.836   0.885   1.020   1.663   1.715   1.864
173   T        -0.640  -0.456  -0.771  -0.515  -0.238  -0.811  -0.590  -0.357  -0.724
174   F        -0.868  -0.862  -0.873  -0.497  -0.488  -0.484  -0.891  -0.872  -0.846
175   L         1.499   1.532   1.621   1.591   1.623   1.772   1.663   1.715   1.864
176   K         1.499   1.532   1.621   0.836   0.885   1.020   1.663   1.715   1.864
177   S        -1.236  -1.258  -1.262  -0.756  -0.791  -0.775  -1.041  -1.059  -1.017
178   L         0.739   0.693   0.527   0.983   0.922   0.724   0.762   0.685   0.369
179   V        -1.374  -1.372  -1.309  -1.397  -1.421  -1.261  -1.191  -1.178  -1.062
180   V        -0.363  -0.299  -0.335  -0.175  -0.089  -0.127  -0.590  -0.506  -0.514
181   D        -0.273  -0.244  -0.292  -0.157  -0.116  -0.199  -0.440  -0.378  -0.448
182   S        -0.501  -0.467  -0.384  -0.957  -0.905  -0.698  -0.741  -0.694  -0.575
183   -        -1.071  -1.053  -0.925  -0.836  -0.820  -0.646  -1.097  -1.071  -0.899
184   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
185   E        -0.501  -0.506  -0.389  -0.868  -0.987  -0.832  -0.741  -0.746  -0.582
186   G         0.739   0.795   0.547   1.062   1.135   0.795   0.762   0.850   0.406
187   Q        -1.741  -1.723  -1.640  -1.834  -1.811  -1.613  -1.342  -1.319  -1.184
188   I         0.023  -0.139  -0.266   0.406   0.192  -0.008   0.011  -0.219  -0.406
189   R        -1.236  -1.213  -1.264  -0.717  -0.703  -0.776  -1.041  -1.015  -1.019
190   Y        -0.868  -0.833  -0.851  -0.542  -0.512  -0.509  -0.891  -0.855  -0.825
191   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
192   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
193   A        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
194   K        -0.971  -1.007  -0.910  -1.417  -1.428  -1.236  -0.987  -1.022  -0.883
195   E        -1.741  -1.739  -1.645  -1.515  -1.625  -1.419  -1.342  -1.332  -1.188
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P37263
Output conservation file - output/P37263
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
