1     M         1.064   1.080   1.132   1.272   1.292   1.359   1.138   1.158   1.233
2     V        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
3     S        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
4     T        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
5     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
6     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
7     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
8     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
9     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
10    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
11    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
12    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
13    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
14    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
15    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
16    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
17    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
18    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
19    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
20    T        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
21    Q        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
22    S         1.064   1.080   1.132   1.097   1.133   1.202   1.138   1.158   1.233
23    R        -0.331  -0.493  -0.674  -0.193  -0.424  -0.680  -0.304  -0.544  -0.762
24    S        -1.318  -1.182  -1.243  -1.268  -1.079  -1.148  -1.301  -1.160  -1.136
25    L        -2.223  -2.119  -1.983  -2.213  -1.986  -1.820  -1.745  -1.642  -1.438
26    K        -0.143  -0.126  -0.069  -0.284  -0.243  -0.174  -0.439  -0.410  -0.355
27    A        -1.293  -1.128  -1.244  -1.111  -0.875  -1.085  -1.227  -1.030  -1.132
28    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
29    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
30    M        -2.223  -2.196  -1.981  -2.141  -2.189  -1.812  -1.745  -1.706  -1.437
31    G         0.349   0.436   0.061   0.504   0.608   0.077   0.362   0.484  -0.123
32    E        -0.003   0.088  -0.010  -0.161  -0.028  -0.202  -0.193  -0.042  -0.247
33    E        -0.784  -0.702  -0.742  -1.009  -0.944  -0.894  -0.969  -0.887  -0.858
34    I        -0.231  -0.230  -0.082  -0.570  -0.577  -0.327  -0.600  -0.600  -0.378
35    W         0.286   0.163   0.018   0.632   0.473   0.215   0.269   0.079  -0.198
36    K         1.064   1.080   1.132   1.003   1.024   1.118   1.138   1.158   1.233
37    N        -0.071  -0.192  -0.066  -0.170  -0.389  -0.195  -0.310  -0.529  -0.349
38    K        -0.908  -0.912  -0.765  -1.042  -1.028  -0.824  -1.080  -1.082  -0.882
39    T        -0.644  -0.758  -0.717  -0.709  -0.882  -0.820  -0.747  -0.868  -0.821
40    E        -0.644  -0.553  -0.729  -0.765  -0.670  -0.921  -0.747  -0.644  -0.839
41    K         1.064   1.080   1.132   1.027   1.067   1.140   1.138   1.158   1.233
42    I        -0.251  -0.205  -0.078  -0.611  -0.534  -0.320  -0.636  -0.553  -0.372
43    N         1.064   1.080   1.132   1.075   1.108   1.182   1.138   1.158   1.233
44    T        -0.784  -0.720  -0.738  -0.819  -0.744  -0.719  -0.969  -0.905  -0.854
45    E         1.064   1.080   1.132   0.993   1.006   1.110   1.138   1.158   1.233
46    L         1.064   1.080   1.132   0.819   0.852   0.954   1.138   1.158   1.233
47    F         0.349   0.078   0.045   0.507   0.109   0.024   0.362  -0.059  -0.151
48    T        -1.237  -1.221  -1.251  -1.195  -1.261  -1.260  -1.190  -1.200  -1.143
49    L         1.064   1.080   1.132   0.819   0.852   0.954   1.138   1.158   1.233
50    T         1.064   1.080   1.132   1.129   1.144   1.231   1.138   1.158   1.233
51    Y         1.064   1.080   1.132   1.265   1.288   1.353   1.138   1.158   1.233
52    G         1.064   1.080   1.132   1.126   1.143   1.228   1.138   1.158   1.233
53    S         0.349   0.078   0.045   0.480   0.119   0.037   0.362  -0.059  -0.151
54    I        -0.003   0.088  -0.010  -0.127   0.027  -0.175  -0.193  -0.042  -0.247
55    V         1.064   1.080   1.132   0.990   1.000   1.106   1.138   1.158   1.233
56    A        -0.191  -0.078  -0.059  -0.301  -0.099  -0.113  -0.525  -0.325  -0.337
57    Q         1.064   1.080   1.132   1.215   1.238   1.308   1.138   1.158   1.233
58    L         0.349   0.078   0.045   0.187  -0.180  -0.235   0.362  -0.059  -0.151
59    C         1.064   1.080   1.132   1.290   1.317   1.375   1.138   1.158   1.233
60    Q        -1.442  -1.250  -1.264  -1.441  -1.156  -1.181  -1.412  -1.193  -1.154
61    D         1.064   1.080   1.132   0.997   1.007   1.113   1.138   1.158   1.233
62    Y        -0.644  -0.779  -0.733  -0.401  -0.631  -0.563  -0.747  -0.932  -0.844
63    E        -1.500  -1.409  -1.280  -1.596  -1.481  -1.271  -1.469  -1.368  -1.170
64    R        -0.841  -0.798  -0.758  -0.906  -0.835  -0.763  -1.034  -0.959  -0.875
65    D        -0.191  -0.157  -0.071  -0.465  -0.412  -0.275  -0.525  -0.465  -0.359
66    F        -0.191  -0.070  -0.052  -0.193   0.029  -0.004  -0.525  -0.310  -0.324
67    N        -2.552  -2.519  -2.269  -2.450  -2.368  -2.066  -1.856  -1.813  -1.526
68    K        -0.784  -0.772  -0.747  -0.976  -0.969  -0.867  -0.969  -0.952  -0.864
69    V         1.064   1.080   1.132   0.990   1.000   1.106   1.138   1.158   1.233
70    N         1.064   1.080   1.132   1.073   1.102   1.181   1.138   1.158   1.233
71    D        -1.876  -1.846  -1.667  -1.921  -1.887  -1.599  -1.614  -1.567  -1.331
72    H        -0.447  -0.515  -0.704  -0.136  -0.246  -0.545  -0.455  -0.548  -0.802
73    L         1.064   1.080   1.132   0.808   0.836   0.944   1.138   1.158   1.233
74    Y        -0.749  -0.637  -0.740  -0.779  -0.615  -0.732  -0.890  -0.727  -0.850
75    S        -0.841  -0.779  -0.753  -1.005  -0.916  -0.829  -1.034  -0.949  -0.869
76    M         1.064   1.080   1.132   1.274   1.294   1.360   1.138   1.158   1.233
77    G         1.064   1.080   1.132   1.130   1.147   1.232   1.138   1.158   1.233
78    Y         1.064   1.080   1.132   1.262   1.281   1.350   1.138   1.158   1.233
79    N         1.064   1.080   1.132   1.073   1.102   1.181   1.138   1.158   1.233
80    I         1.064   1.080   1.132   1.019   1.047   1.133   1.138   1.158   1.233
81    G         1.064   1.080   1.132   1.130   1.147   1.232   1.138   1.158   1.233
82    C        -0.841  -0.794  -0.757  -1.171  -1.102  -0.947  -1.034  -0.973  -0.874
83    R         1.064   1.080   1.132   1.178   1.198   1.275   1.138   1.158   1.233
84    L         1.064   1.080   1.132   0.808   0.836   0.944   1.138   1.158   1.233
85    I         1.064   1.080   1.132   1.019   1.047   1.133   1.138   1.158   1.233
86    E         1.064   1.080   1.132   0.998   1.015   1.114   1.138   1.158   1.233
87    D        -0.071  -0.014  -0.032  -0.252  -0.162  -0.220  -0.310  -0.213  -0.287
88    F         0.286   0.225   0.021   0.471   0.395   0.088   0.269   0.175  -0.193
89    L        -0.447  -0.458  -0.699  -0.590  -0.598  -0.942  -0.455  -0.468  -0.795
90    A         1.064   1.080   1.132   1.178   1.191   1.275   1.138   1.158   1.233
91    R        -0.231  -0.175  -0.069  -0.471  -0.382  -0.207  -0.600  -0.499  -0.355
92    T         1.064   1.080   1.132   1.142   1.169   1.242   1.138   1.158   1.233
93    A        -1.358  -1.444  -1.277  -1.291  -1.435  -1.212  -1.324  -1.387  -1.167
94    L        -1.876  -1.905  -1.671  -1.934  -1.918  -1.558  -1.614  -1.615  -1.334
95    P        -1.125  -1.108  -1.240  -0.740  -0.723  -0.967  -1.034  -1.007  -1.129
96    R         1.064   1.080   1.132   1.178   1.198   1.275   1.138   1.158   1.233
97    C         1.064   1.080   1.132   1.285   1.311   1.371   1.138   1.158   1.233
98    E        -1.876  -1.906  -1.677  -1.684  -1.697  -1.393  -1.614  -1.615  -1.339
99    N        -0.841  -0.900  -0.758  -0.953  -1.025  -0.821  -1.034  -1.078  -0.876
100   L        -0.447  -0.331  -0.692  -0.263  -0.103  -0.688  -0.455  -0.297  -0.786
101   V        -1.358  -1.352  -1.266  -1.536  -1.499  -1.406  -1.324  -1.297  -1.157
102   K        -1.734  -1.648  -1.653  -1.665  -1.553  -1.564  -1.469  -1.371  -1.318
103   T         1.064   1.080   1.132   1.142   1.169   1.242   1.138   1.158   1.233
104   S        -0.841  -0.898  -0.764  -0.890  -0.953  -0.753  -1.034  -1.074  -0.883
105   E         1.064   1.080   1.132   0.998   1.015   1.114   1.138   1.158   1.233
106   V        -1.125  -1.232  -1.247  -1.034  -1.192  -1.223  -1.034  -1.151  -1.135
107   L        -0.749  -0.767  -0.756  -0.956  -1.017  -1.025  -0.890  -0.930  -0.872
108   S         0.286   0.163   0.018   0.401   0.244   0.005   0.269   0.079  -0.198
109   K         1.064   1.080   1.132   1.003   1.024   1.118   1.138   1.158   1.233
110   C        -0.841  -0.790  -0.755  -0.950  -0.892  -0.866  -1.034  -0.961  -0.871
111   A        -0.191  -0.070  -0.052  -0.301  -0.100  -0.095  -0.525  -0.310  -0.324
112   F         1.064   1.080   1.132   1.144   1.163   1.244   1.138   1.158   1.233
113   K         0.349   0.421   0.056   0.417   0.524   0.017   0.362   0.464  -0.130
114   I        -0.003   0.037  -0.046  -0.127  -0.058  -0.247  -0.193  -0.128  -0.312
115   F         1.064   1.080   1.132   1.144   1.163   1.244   1.138   1.158   1.233
116   L         1.064   1.080   1.132   0.819   0.852   0.954   1.138   1.158   1.233
117   N         1.064   1.080   1.132   1.075   1.108   1.182   1.138   1.158   1.233
118   I        -0.972  -1.209  -1.231  -0.806  -1.180  -1.209  -0.858  -1.135  -1.120
119   T        -0.644  -0.614  -0.717  -0.532  -0.478  -0.653  -0.747  -0.684  -0.820
120   P         1.064   1.080   1.132   1.258   1.276   1.346   1.138   1.158   1.233
121   N        -2.223  -2.072  -1.978  -2.000  -1.811  -1.650  -1.745  -1.615  -1.435
122   I        -0.251  -0.202  -0.078  -0.611  -0.513  -0.315  -0.636  -0.549  -0.370
123   T        -0.972  -0.946  -1.206  -0.710  -0.680  -1.099  -0.858  -0.819  -1.093
124   N         0.349   0.305   0.046   0.453   0.414   0.009   0.362   0.298  -0.148
125   W         0.349   0.305   0.046   0.674   0.619   0.167   0.362   0.298  -0.148
126   S        -0.331  -0.559  -0.685  -0.120  -0.440  -0.635  -0.304  -0.620  -0.776
127   H        -1.770  -1.827  -1.645  -1.511  -1.615  -1.343  -1.523  -1.555  -1.314
128   N        -0.972  -0.686  -1.194  -0.832  -0.469  -1.163  -0.858  -0.516  -1.080
129   K        -1.237  -1.256  -1.240  -1.139  -1.168  -1.146  -1.190  -1.200  -1.130
130   D        -0.908  -0.789  -0.769  -1.123  -0.901  -0.922  -1.080  -0.912  -0.887
131   T        -0.989  -0.997  -0.788  -1.081  -1.071  -0.773  -1.190  -1.188  -0.911
132   F         0.349   0.421   0.056   0.550   0.635   0.155   0.362   0.464  -0.130
133   S         0.349   0.305   0.046   0.460   0.417  -0.013   0.362   0.298  -0.148
134   L         1.064   1.080   1.132   0.819   0.852   0.954   1.138   1.158   1.233
135   I        -0.972  -1.140  -1.215  -0.871  -1.145  -1.310  -0.858  -1.067  -1.102
136   L        -0.989  -0.918  -0.784  -1.342  -1.193  -1.011  -1.190  -1.094  -0.907
137   D        -0.972  -0.836  -1.202  -0.843  -0.676  -1.196  -0.858  -0.690  -1.089
138   E        -0.003   0.017  -0.046  -0.161  -0.146  -0.273  -0.193  -0.161  -0.313
139   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
140   N         1.064   1.080   1.132   1.075   1.108   1.182   1.138   1.158   1.233
141   P         1.064   1.080   1.132   1.258   1.276   1.346   1.138   1.158   1.233
142   L         1.064   1.080   1.132   0.819   0.852   0.954   1.138   1.158   1.233
143   A        -0.784  -0.673  -0.732  -0.804  -0.624  -0.703  -0.969  -0.811  -0.845
144   D        -0.191  -0.157  -0.071  -0.508  -0.465  -0.323  -0.525  -0.465  -0.359
145   F         1.064   1.080   1.132   1.144   1.163   1.244   1.138   1.158   1.233
146   V         1.064   1.080   1.132   0.990   1.000   1.106   1.138   1.158   1.233
147   E         1.064   1.080   1.132   0.993   1.006   1.110   1.138   1.158   1.233
148   L         1.064   1.080   1.132   0.819   0.852   0.954   1.138   1.158   1.233
149   P         1.064   1.080   1.132   1.258   1.276   1.346   1.138   1.158   1.233
150   M        -1.293  -1.337  -1.274  -1.065  -1.143  -1.143  -1.227  -1.270  -1.163
151   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
152   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
153   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
154   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
155   D        -0.908  -0.927  -0.772  -1.089  -1.112  -0.876  -1.080  -1.103  -0.890
156   A        -0.003   0.088  -0.010   0.020   0.160  -0.044  -0.193  -0.042  -0.247
157   M        -2.881  -2.774  -2.509  -2.353  -2.181  -1.883  -1.966  -1.879  -1.587
158   K        -0.972  -1.140  -1.215  -0.805  -1.071  -1.177  -0.858  -1.067  -1.102
159   S        -1.237  -1.260  -1.244  -1.210  -1.254  -1.206  -1.190  -1.226  -1.136
160   L         1.064   1.080   1.132   0.819   0.852   0.954   1.138   1.158   1.233
161   W         1.064   1.080   1.132   1.311   1.335   1.393   1.138   1.158   1.233
162   Y         0.349   0.078   0.045   0.649   0.275   0.181   0.362  -0.059  -0.151
163   S         1.064   1.080   1.132   1.097   1.133   1.202   1.138   1.158   1.233
164   N        -0.003   0.088  -0.010  -0.011   0.137  -0.038  -0.193  -0.042  -0.247
165   I         0.349   0.381   0.048   0.394   0.446  -0.066   0.362   0.407  -0.145
166   L         1.064   1.080   1.132   0.819   0.852   0.954   1.138   1.158   1.233
167   C         0.349   0.381   0.048   0.677   0.723   0.216   0.362   0.407  -0.145
168   G         1.064   1.080   1.132   1.126   1.143   1.228   1.138   1.158   1.233
169   V         1.064   1.080   1.132   0.990   1.000   1.106   1.138   1.158   1.233
170   L        -0.003  -0.012  -0.050  -0.321  -0.325  -0.398  -0.193  -0.210  -0.320
171   K        -0.191  -0.070  -0.052  -0.328  -0.091  -0.127  -0.525  -0.310  -0.324
172   G         1.064   1.080   1.132   1.126   1.143   1.228   1.138   1.158   1.233
173   S        -0.003   0.076  -0.045   0.041   0.159  -0.085  -0.193  -0.063  -0.311
174   L         1.064   1.080   1.132   0.819   0.852   0.954   1.138   1.158   1.233
175   E        -0.003   0.088  -0.010  -0.089   0.037  -0.102  -0.193  -0.042  -0.247
176   M         1.064   1.080   1.132   1.272   1.292   1.359   1.138   1.158   1.233
177   V         0.349   0.305   0.046   0.329   0.260  -0.168   0.362   0.298  -0.148
178   Q         1.064   1.080   1.132   1.215   1.238   1.308   1.138   1.158   1.233
179   L        -0.191  -0.231  -0.081  -0.511  -0.523  -0.279  -0.525  -0.601  -0.377
180   D        -0.331  -0.559  -0.685  -0.234  -0.561  -0.745  -0.304  -0.620  -0.776
181   C        -0.251  -0.199  -0.077  -0.419  -0.370  -0.158  -0.636  -0.542  -0.369
182   D        -0.191  -0.218  -0.078  -0.508  -0.591  -0.337  -0.525  -0.577  -0.371
183   V        -0.644  -0.865  -0.745  -0.675  -1.005  -0.803  -0.747  -1.014  -0.859
184   W        -2.099  -2.136  -1.977  -1.549  -1.608  -1.450  -1.634  -1.659  -1.434
185   F         1.064   1.080   1.132   1.144   1.163   1.244   1.138   1.158   1.233
186   V        -0.644  -0.767  -0.734  -0.788  -1.012  -0.980  -0.747  -0.914  -0.846
187   S        -0.644  -0.614  -0.717  -0.618  -0.530  -0.730  -0.747  -0.684  -0.820
188   D         1.064   1.080   1.132   0.997   1.007   1.113   1.138   1.158   1.233
189   I        -1.318  -1.238  -1.242  -1.424  -1.322  -1.262  -1.301  -1.209  -1.135
190   L         1.064   1.080   1.132   0.819   0.852   0.954   1.138   1.158   1.233
191   R         1.064   1.080   1.132   1.180   1.200   1.276   1.138   1.158   1.233
192   G         1.064   1.080   1.132   1.126   1.143   1.228   1.138   1.158   1.233
193   D        -0.331  -0.474  -0.669  -0.193  -0.389  -0.631  -0.304  -0.522  -0.756
194   S        -1.770  -1.552  -1.638  -1.736  -1.470  -1.510  -1.523  -1.314  -1.308
195   Q        -1.237  -1.294  -1.251  -0.957  -1.078  -0.970  -1.190  -1.260  -1.143
196   T         1.064   1.080   1.132   1.129   1.144   1.231   1.138   1.158   1.233
197   E         1.064   1.080   1.132   0.993   1.006   1.110   1.138   1.158   1.233
198   I        -0.784  -0.937  -0.739  -1.103  -1.238  -0.929  -0.969  -1.116  -0.855
199   K         0.349   0.457   0.056   0.548   0.683   0.089   0.362   0.514  -0.130
200   V        -0.644  -0.470  -0.704  -0.829  -0.566  -0.939  -0.747  -0.514  -0.803
201   K        -0.972  -1.209  -1.231  -0.726  -1.049  -1.048  -0.858  -1.135  -1.120
202   L         1.064   1.080   1.132   0.819   0.852   0.954   1.138   1.158   1.233
203   N        -0.644  -0.787  -0.745  -0.682  -0.942  -0.880  -0.747  -0.946  -0.858
204   R        -0.251  -0.209  -0.080  -0.474  -0.372  -0.195  -0.636  -0.561  -0.376
205   I        -0.644  -0.750  -0.715  -0.678  -0.773  -0.762  -0.747  -0.858  -0.818
206   L         0.349   0.078   0.045   0.187  -0.180  -0.235   0.362  -0.059  -0.151
207   K        -0.331  -0.559  -0.685  -0.241  -0.580  -0.808  -0.304  -0.620  -0.776
208   D         0.349   0.305   0.046   0.361   0.291  -0.095   0.362   0.298  -0.148
209   E         1.064   1.080   1.132   0.993   1.006   1.110   1.138   1.158   1.233
210   I        -1.237  -1.282  -1.236  -1.287  -1.413  -1.291  -1.190  -1.233  -1.125
211   P         1.064   1.080   1.132   1.258   1.276   1.346   1.138   1.158   1.233
212   I        -1.442  -1.397  -1.275  -1.438  -1.342  -1.228  -1.412  -1.355  -1.166
213   G         0.349   0.078   0.045   0.490   0.090   0.010   0.362  -0.059  -0.151
214   E        -0.003  -0.161  -0.058  -0.161  -0.475  -0.298  -0.193  -0.474  -0.335
215   D         0.349   0.305   0.046   0.361   0.291  -0.095   0.362   0.298  -0.148
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P36149
Output conservation file - output/P36149
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
