1     M         1.079   1.130   1.209   1.230   1.302   1.417   1.151   1.221   1.341
2     R         0.348   0.560   0.118   0.331   0.590   0.041   0.345   0.641  -0.055
3     I        -0.205  -0.183  -0.032  -0.506  -0.526  -0.281  -0.577  -0.559  -0.327
4     Y         1.079   1.130   1.209   1.102   1.180   1.301   1.151   1.221   1.341
5     Y         0.348   0.261   0.092   0.456   0.345   0.042   0.345   0.208  -0.101
6     I         0.348   0.261   0.092   0.409   0.310   0.040   0.345   0.208  -0.101
7     G         0.348   0.261   0.092   0.486   0.383   0.067   0.345   0.208  -0.101
8     V        -0.205   0.013   0.027  -0.438  -0.067  -0.076  -0.577  -0.203  -0.217
9     F        -0.668  -0.651  -0.679  -0.741  -0.702  -0.726  -0.808  -0.750  -0.781
10    R         0.348   0.138   0.074   0.493   0.192   0.053   0.345   0.008  -0.132
11    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
12    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
13    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
14    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
15    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
16    S        -1.821  -1.760  -1.655  -1.694  -1.564  -1.391  -1.614  -1.514  -1.320
17    G        -1.821  -1.869  -1.666  -1.761  -1.809  -1.497  -1.614  -1.593  -1.329
18    G        -1.821  -1.870  -1.671  -1.655  -1.740  -1.476  -1.614  -1.593  -1.332
19    E        -1.821  -1.880  -1.668  -1.772  -1.859  -1.535  -1.614  -1.602  -1.330
20    K        -0.668  -0.764  -0.735  -0.715  -0.851  -0.786  -0.808  -0.949  -0.858
21    A        -0.349  -0.319  -0.642  -0.215  -0.198  -0.649  -0.347  -0.314  -0.730
22    L        -0.668  -0.814  -0.735  -0.804  -1.038  -0.890  -0.808  -0.996  -0.859
23    E        -0.668  -0.814  -0.735  -0.772  -1.017  -0.906  -0.808  -0.996  -0.859
24    L         1.079   1.130   1.209   0.909   0.989   1.127   1.151   1.221   1.341
25    S        -1.484  -1.368  -1.264  -1.496  -1.249  -1.154  -1.499  -1.331  -1.157
26    E        -1.275  -1.337  -1.235  -1.379  -1.414  -1.280  -1.269  -1.285  -1.127
27    V        -0.205   0.013   0.027  -0.447  -0.092  -0.084  -0.577  -0.203  -0.217
28    K        -1.484  -1.470  -1.271  -1.599  -1.543  -1.238  -1.499  -1.428  -1.164
29    D         0.348   0.138   0.074   0.413   0.117  -0.008   0.345   0.008  -0.132
30    L         1.079   1.130   1.209   0.909   0.989   1.127   1.151   1.221   1.341
31    S         1.079   1.130   1.209   0.892   0.970   1.111   1.151   1.221   1.341
32    Q        -0.668  -0.862  -0.738  -0.731  -1.074  -0.894  -0.808  -1.042  -0.862
33    F         0.348   0.358   0.097   0.551   0.573   0.185   0.345   0.357  -0.092
34    G        -0.205   0.013   0.027  -0.514  -0.168  -0.147  -0.577  -0.203  -0.217
35    F        -0.349  -0.563  -0.682  -0.013  -0.305  -0.461  -0.347  -0.638  -0.784
36    F         1.079   1.130   1.209   1.168   1.242   1.361   1.151   1.221   1.341
37    E        -0.349  -0.643  -0.690  -0.272  -0.669  -0.772  -0.347  -0.739  -0.795
38    R         1.079   1.130   1.209   1.132   1.202   1.328   1.151   1.221   1.341
39    S        -0.812  -0.893  -0.727  -0.909  -1.052  -0.799  -1.038  -1.087  -0.849
40    S        -1.275  -1.314  -1.246  -1.322  -1.374  -1.253  -1.269  -1.265  -1.138
41    V        -0.349  -0.643  -0.690  -0.195  -0.605  -0.703  -0.347  -0.739  -0.795
42    G        -1.275  -1.210  -1.231  -1.242  -1.153  -1.229  -1.269  -1.165  -1.122
43    Q        -0.349  -0.643  -0.690  -0.188  -0.597  -0.692  -0.347  -0.739  -0.795
44    F         0.348   0.358   0.097   0.540   0.559   0.156   0.345   0.357  -0.092
45    M         0.348   0.358   0.097   0.622   0.643   0.260   0.345   0.357  -0.092
46    T        -0.349  -0.319  -0.642  -0.184  -0.134  -0.628  -0.347  -0.314  -0.730
47    F         0.348   0.138   0.074   0.569   0.304   0.196   0.345   0.008  -0.132
48    F        -0.349  -0.563  -0.682  -0.098  -0.418  -0.667  -0.347  -0.638  -0.784
49    A        -0.812  -0.829  -0.718  -1.044  -1.077  -0.881  -1.038  -1.019  -0.839
50    E        -0.349  -0.643  -0.690  -0.276  -0.672  -0.782  -0.347  -0.739  -0.795
51    T         1.079   1.130   1.209   1.059   1.144   1.262   1.151   1.221   1.341
52    V         1.079   1.130   1.209   1.039   1.115   1.244   1.151   1.221   1.341
53    A        -0.668  -0.519  -0.680  -0.750  -0.511  -0.788  -0.808  -0.575  -0.782
54    S        -2.284  -1.945  -1.991  -2.151  -1.681  -1.737  -1.845  -1.515  -1.440
55    R         1.079   1.130   1.209   1.132   1.202   1.328   1.151   1.221   1.341
56    T         1.079   1.130   1.209   1.059   1.144   1.262   1.151   1.221   1.341
57    G        -2.620  -2.450  -2.290  -2.418  -2.219  -1.990  -1.960  -1.780  -1.535
58    A        -0.266  -0.122  -0.011  -0.435  -0.179  -0.017  -0.692  -0.446  -0.288
59    G         1.079   1.130   1.209   1.132   1.218   1.328   1.151   1.221   1.341
60    Q         1.079   1.130   1.209   1.042   1.110   1.247   1.151   1.221   1.341
61    R         1.079   1.130   1.209   1.132   1.202   1.328   1.151   1.221   1.341
62    Q         1.079   1.130   1.209   1.042   1.110   1.247   1.151   1.221   1.341
63    S         0.348   0.261   0.092   0.267   0.165  -0.077   0.345   0.208  -0.101
64    I        -0.012   0.220   0.041  -0.096   0.251  -0.049  -0.231   0.143  -0.192
65    E         1.079   1.130   1.209   0.968   1.062   1.180   1.151   1.221   1.341
66    E         0.348   0.261   0.092   0.342   0.255  -0.016   0.345   0.208  -0.101
67    G        -1.004  -1.269  -1.248  -0.717  -1.138  -1.147  -0.923  -1.214  -1.140
68    N        -0.012  -0.108  -0.028   0.052  -0.154  -0.064  -0.231  -0.420  -0.318
69    Y         1.079   1.130   1.209   1.102   1.180   1.301   1.151   1.221   1.341
70    I        -0.205  -0.157  -0.036  -0.438  -0.376  -0.196  -0.577  -0.510  -0.333
71    G        -0.349  -0.643  -0.690  -0.092  -0.496  -0.606  -0.347  -0.739  -0.795
72    H         1.079   1.130   1.209   1.279   1.347   1.461   1.151   1.221   1.341
73    V        -0.939  -0.859  -0.749  -1.085  -1.003  -0.830  -1.153  -1.047  -0.875
74    Y         1.079   1.130   1.209   1.102   1.180   1.301   1.151   1.221   1.341
75    A        -1.275  -1.237  -1.235  -1.235  -1.166  -1.182  -1.269  -1.175  -1.126
76    R         1.079   1.130   1.209   1.132   1.202   1.328   1.151   1.221   1.341
77    S         0.348   0.511   0.112   0.293   0.492   0.019   0.345   0.576  -0.066
78    E        -0.012  -0.048  -0.013  -0.161  -0.224  -0.197  -0.231  -0.312  -0.290
79    G         1.079   1.130   1.209   1.132   1.218   1.328   1.151   1.221   1.341
80    I        -0.812  -0.892  -0.727  -1.029  -1.140  -0.885  -1.038  -1.086  -0.849
81    C        -1.004  -0.778  -1.186  -0.582  -0.291  -0.953  -0.923  -0.644  -1.074
82    G         0.348   0.529   0.114   0.541   0.766   0.249   0.345   0.599  -0.062
83    V         0.348   0.510   0.113   0.412   0.618   0.079   0.345   0.574  -0.063
84    L        -0.812  -0.826  -0.715  -1.029  -0.984  -0.840  -1.038  -0.990  -0.830
85    I         0.348   0.261   0.092   0.408   0.305   0.035   0.345   0.208  -0.101
86    T         0.348   0.261   0.092   0.412   0.309   0.005   0.345   0.208  -0.101
87    D         1.079   1.130   1.209   1.057   1.129   1.261   1.151   1.221   1.341
88    K         0.348   0.358   0.097   0.318   0.346  -0.028   0.345   0.357  -0.092
89    E        -0.205  -0.150  -0.034  -0.492  -0.409  -0.234  -0.577  -0.497  -0.330
90    Y         1.079   1.130   1.209   1.102   1.180   1.301   1.151   1.221   1.341
91    P         1.079   1.130   1.209   1.217   1.278   1.405   1.151   1.221   1.341
92    V        -0.349  -0.643  -0.690  -0.196  -0.605  -0.705  -0.347  -0.739  -0.795
93    R         0.348   0.138   0.074   0.506   0.216   0.089   0.345   0.008  -0.132
94    P        -0.668  -0.862  -0.738  -0.515  -0.866  -0.705  -0.808  -1.042  -0.862
95    A         1.079   1.130   1.209   1.025   1.090   1.232   1.151   1.221   1.341
96    Y         0.348   0.138   0.074   0.511   0.257   0.162   0.345   0.008  -0.132
97    T         0.348   0.138   0.074   0.412   0.121  -0.029   0.345   0.008  -0.132
98    L         0.348   0.138   0.074   0.283   0.006  -0.096   0.345   0.008  -0.132
99    L        -0.012   0.188   0.053  -0.222   0.081  -0.135  -0.231   0.092  -0.171
100   N         0.348   0.138   0.074   0.542   0.233   0.080   0.345   0.008  -0.132
101   K         1.079   1.130   1.209   0.944   1.025   1.158   1.151   1.221   1.341
102   I        -0.812  -0.872  -0.725  -0.975  -1.059  -0.845  -1.038  -1.057  -0.846
103   L         0.348   0.138   0.074   0.312   0.059  -0.016   0.345   0.008  -0.132
104   D        -0.205  -0.084  -0.017  -0.464  -0.263  -0.188  -0.577  -0.377  -0.297
105   E         0.348   0.138   0.074   0.342   0.077  -0.046   0.345   0.008  -0.132
106   Y        -0.012  -0.138  -0.031   0.008  -0.214  -0.061  -0.231  -0.475  -0.324
107   L         1.079   1.130   1.209   0.909   0.989   1.127   1.151   1.221   1.341
108   V        -1.021  -0.927  -0.757  -1.328  -1.191  -0.925  -1.269  -1.124  -0.884
109   A        -1.484  -1.470  -1.271  -1.711  -1.703  -1.411  -1.499  -1.428  -1.164
110   H        -0.349  -0.643  -0.690   0.041  -0.401  -0.525  -0.347  -0.739  -0.795
111   P         1.079   1.130   1.209   1.217   1.278   1.405   1.151   1.221   1.341
112   K        -2.157  -2.032  -1.983  -1.945  -1.827  -1.698  -1.730  -1.594  -1.434
113   E        -2.620  -2.254  -2.270  -2.531  -1.977  -2.072  -1.960  -1.631  -1.523
114   E        -1.948  -1.915  -1.674  -1.998  -1.948  -1.611  -1.730  -1.629  -1.334
115   W         0.348   0.138   0.074   0.708   0.414   0.258   0.345   0.008  -0.132
116   A        -1.015  -0.780  -0.745  -1.334  -0.942  -0.922  -1.257  -0.925  -0.868
117   D        -0.939  -0.752  -0.733  -1.016  -0.732  -0.769  -1.153  -0.891  -0.853
118   V        -1.015  -0.887  -0.759  -1.249  -1.084  -0.873  -1.257  -1.076  -0.886
119   T        -1.784  -1.762  -1.679  -1.703  -1.750  -1.649  -1.558  -1.512  -1.339
120   E        -1.015  -0.890  -0.762  -1.355  -1.182  -0.975  -1.257  -1.084  -0.890
121   T        -1.821  -1.833  -1.667  -1.706  -1.699  -1.492  -1.614  -1.573  -1.329
122   N        -1.275  -1.357  -1.250  -1.132  -1.321  -1.190  -1.269  -1.325  -1.144
123   D        -2.157  -2.136  -2.008  -1.972  -1.982  -1.835  -1.730  -1.669  -1.452
124   A        -1.821  -1.782  -1.660  -1.885  -1.838  -1.637  -1.614  -1.537  -1.324
125   L        -0.349  -0.416  -0.659  -0.313  -0.434  -0.726  -0.347  -0.464  -0.754
126   K        -2.157  -2.136  -2.008  -1.972  -1.977  -1.755  -1.730  -1.669  -1.452
127   M        -1.484  -1.304  -1.260  -1.352  -1.113  -1.074  -1.499  -1.285  -1.153
128   K        -1.821  -1.374  -1.629  -1.621  -0.967  -1.365  -1.614  -1.131  -1.297
129   Q        -1.275  -1.185  -1.228  -1.269  -1.152  -1.178  -1.269  -1.138  -1.121
130   L         0.348   0.138   0.074   0.283   0.006  -0.098   0.345   0.008  -0.132
131   D        -0.812  -0.588  -0.709  -1.029  -0.721  -0.866  -1.038  -0.764  -0.827
132   T        -2.157  -2.024  -1.989  -1.849  -1.730  -1.646  -1.730  -1.581  -1.439
133   Y         1.079   1.130   1.209   1.102   1.180   1.301   1.151   1.221   1.341
134   I        -0.812  -0.757  -0.714  -1.029  -0.964  -0.867  -1.038  -0.937  -0.831
135   S        -1.357  -1.330  -1.250  -1.554  -1.498  -1.332  -1.384  -1.314  -1.143
136   K        -0.349  -0.563  -0.682  -0.282  -0.582  -0.743  -0.347  -0.638  -0.784
137   Y         1.079   1.130   1.209   1.102   1.180   1.301   1.151   1.221   1.341
138   Q         1.079   1.130   1.209   1.042   1.110   1.247   1.151   1.221   1.341
139   D         1.079   1.130   1.209   1.049   1.116   1.253   1.151   1.221   1.341
140   P         0.348   0.138   0.074   0.589   0.283   0.156   0.345   0.008  -0.132
141   S        -2.157  -2.113  -1.998  -1.978  -1.914  -1.723  -1.730  -1.655  -1.445
142   Q         0.348   0.138   0.074   0.438   0.165   0.076   0.345   0.008  -0.132
143   A         1.079   1.130   1.209   1.025   1.090   1.232   1.151   1.221   1.341
144   D         0.348   0.138   0.074   0.402   0.092  -0.038   0.345   0.008  -0.132
145   A        -0.812  -0.829  -0.728  -1.044  -1.077  -0.894  -1.038  -1.019  -0.851
146   I         1.079   1.130   1.209   1.035   1.116   1.241   1.151   1.221   1.341
147   M         0.348   0.138   0.074   0.602   0.307   0.167   0.345   0.008  -0.132
148   K        -0.205  -0.157  -0.036  -0.468  -0.401  -0.200  -0.577  -0.510  -0.333
149   V         0.348   0.138   0.074   0.412   0.131   0.011   0.345   0.008  -0.132
150   Q         1.079   1.130   1.209   1.042   1.110   1.247   1.151   1.221   1.341
151   Q         1.079   1.130   1.209   1.042   1.110   1.247   1.151   1.221   1.341
152   E         1.079   1.130   1.209   0.968   1.062   1.180   1.151   1.221   1.341
153   L         1.079   1.130   1.209   0.909   0.989   1.127   1.151   1.221   1.341
154   D         1.079   1.130   1.209   1.049   1.116   1.253   1.151   1.221   1.341
155   E         0.348   0.510   0.113   0.343   0.564   0.023   0.345   0.574  -0.063
156   T         1.079   1.130   1.209   1.059   1.144   1.262   1.151   1.221   1.341
157   K         1.079   1.130   1.209   0.944   1.025   1.158   1.151   1.221   1.341
158   I        -0.349  -0.320  -0.642  -0.207  -0.176  -0.645  -0.347  -0.315  -0.730
159   V         0.348   0.511   0.112   0.416   0.622   0.085   0.345   0.576  -0.066
160   L         1.079   1.130   1.209   0.909   0.989   1.127   1.151   1.221   1.341
161   H         1.079   1.130   1.209   1.279   1.347   1.461   1.151   1.221   1.341
162   K         1.079   1.130   1.209   0.944   1.025   1.158   1.151   1.221   1.341
163   T         0.348   0.138   0.074   0.431   0.152   0.024   0.345   0.008  -0.132
164   I         1.079   1.130   1.209   1.035   1.116   1.241   1.151   1.221   1.341
165   E         0.348   0.138   0.074   0.343   0.078  -0.043   0.345   0.008  -0.132
166   N        -0.812  -0.541  -0.681  -0.952  -0.588  -0.720  -1.038  -0.652  -0.785
167   V         1.079   1.130   1.209   1.039   1.115   1.244   1.151   1.221   1.341
168   L         1.079   1.130   1.209   0.909   0.989   1.127   1.151   1.221   1.341
169   Q        -0.012  -0.048  -0.013  -0.096  -0.190  -0.153  -0.231  -0.312  -0.290
170   R         1.079   1.130   1.209   1.132   1.202   1.328   1.151   1.221   1.341
171   G         1.079   1.130   1.209   1.132   1.218   1.328   1.151   1.221   1.341
172   E         1.079   1.130   1.209   0.968   1.062   1.180   1.151   1.221   1.341
173   K         0.348   0.358   0.097   0.331   0.361   0.007   0.345   0.357  -0.092
174   L         1.079   1.130   1.209   0.909   0.989   1.127   1.151   1.221   1.341
175   D         1.079   1.130   1.209   1.049   1.116   1.253   1.151   1.221   1.341
176   N        -1.484  -1.373  -1.265  -1.510  -1.365  -1.218  -1.499  -1.336  -1.157
177   L         1.079   1.130   1.209   0.909   0.989   1.127   1.151   1.221   1.341
178   V         0.348   0.261   0.092   0.412   0.309   0.042   0.345   0.208  -0.101
179   D        -0.349  -0.643  -0.690  -0.197  -0.636  -0.724  -0.347  -0.739  -0.795
180   K         0.348   0.261   0.092   0.331   0.238  -0.001   0.345   0.208  -0.101
181   S         1.079   1.130   1.209   0.892   0.970   1.111   1.151   1.221   1.341
182   E        -0.349  -0.643  -0.690  -0.299  -0.727  -0.840  -0.347  -0.739  -0.795
183   S        -1.357  -1.442  -1.261  -1.472  -1.550  -1.251  -1.384  -1.402  -1.153
184   L         1.079   1.130   1.209   0.909   0.989   1.127   1.151   1.221   1.341
185   T        -0.012   0.251   0.076  -0.231   0.157  -0.100  -0.231   0.192  -0.129
186   A        -1.275  -1.237  -1.235  -1.311  -1.236  -1.259  -1.269  -1.175  -1.126
187   S        -0.668  -0.656  -0.724  -0.822  -0.874  -0.894  -0.808  -0.842  -0.845
188   S         1.079   1.130   1.209   0.892   0.970   1.111   1.151   1.221   1.341
189   K        -0.266  -0.182  -0.037  -0.556  -0.437  -0.200  -0.692  -0.558  -0.335
190   M         0.348   0.358   0.097   0.603   0.623   0.212   0.345   0.357  -0.092
191   F         1.079   1.130   1.209   1.168   1.242   1.361   1.151   1.221   1.341
192   Y         0.348   0.358   0.097   0.495   0.522   0.154   0.345   0.357  -0.092
193   K         0.348   0.138   0.074   0.318   0.040  -0.066   0.345   0.008  -0.132
194   Q        -0.012  -0.138  -0.031  -0.139  -0.424  -0.269  -0.231  -0.475  -0.324
195   A         1.079   1.130   1.209   1.025   1.090   1.232   1.151   1.221   1.341
196   K         1.079   1.130   1.209   0.944   1.025   1.158   1.151   1.221   1.341
197   K         1.079   1.130   1.209   0.944   1.025   1.158   1.151   1.221   1.341
198   S        -0.668  -0.620  -0.720  -0.688  -0.704  -0.825  -0.808  -0.808  -0.840
199   N         1.079   1.130   1.209   1.188   1.259   1.379   1.151   1.221   1.341
200   S         1.079   1.130   1.209   0.892   0.970   1.111   1.151   1.221   1.341
201   C         1.079   1.130   1.209   1.285   1.351   1.467   1.151   1.221   1.341
202   C         1.079   1.130   1.209   1.285   1.351   1.467   1.151   1.221   1.341
203   I        -0.349  -0.432  -0.660  -0.230  -0.385  -0.730  -0.347  -0.482  -0.755
204   I        -0.668  -0.716  -0.720  -0.708  -0.808  -0.837  -0.808  -0.871  -0.840
205   M         0.348   0.261   0.092   0.602   0.489   0.199   0.345   0.208  -0.101
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P36015
Output conservation file - output/P36015
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
