1     M         1.736   1.766   1.819   2.010   2.056   2.145   1.907   1.949   2.028
2     S         1.736   1.766   1.819   1.706   1.754   1.840   1.907   1.949   2.028
3     L         0.431   0.511   0.374   0.296   0.436   0.167   0.340   0.469   0.235
4     Y         0.431   0.338   0.319   0.644   0.496   0.456   0.340   0.183   0.141
5     F         0.431   0.338   0.319   0.623   0.490   0.466   0.340   0.183   0.141
6     T         0.431   0.511   0.374   0.428   0.555   0.346   0.340   0.469   0.235
7     T        -0.677  -0.729  -0.702  -0.781  -0.912  -0.870  -0.705  -0.762  -0.713
8     L         0.431   0.338   0.319   0.089  -0.064  -0.087   0.340   0.183   0.141
9     F         1.736   1.766   1.819   1.857   1.905   1.991   1.907   1.949   2.028
10    L        -1.482  -1.452  -1.416  -1.592  -1.497  -1.480  -1.227  -1.181  -1.115
11    L        -0.677  -0.676  -0.683  -1.001  -0.994  -0.985  -0.705  -0.698  -0.688
12    L         1.736   1.766   1.819   1.401   1.438   1.533   1.907   1.949   2.028
13    T        -0.677  -0.729  -0.702  -0.646  -0.734  -0.704  -0.705  -0.762  -0.713
14    V        -0.677  -0.729  -0.702  -0.842  -0.971  -0.911  -0.705  -0.762  -0.713
15    E         0.431   0.449   0.354   0.508   0.540   0.387   0.340   0.368   0.200
16    M         1.736   1.766   1.819   2.010   2.056   2.145   1.907   1.949   2.028
17    V         0.127   0.152   0.204  -0.042  -0.005   0.082  -0.182  -0.142  -0.063
18    M        -1.482  -1.452  -1.416  -1.244  -1.185  -1.130  -1.227  -1.181  -1.115
19    L         0.431   0.449   0.354   0.186   0.207   0.102   0.340   0.368   0.200
20    F         0.431   0.338   0.319   0.431   0.245   0.181   0.340   0.183   0.141
21    I        -1.482  -1.452  -1.416  -1.556  -1.545  -1.444  -1.227  -1.181  -1.115
22    F        -1.482  -1.452  -1.416  -1.486  -1.388  -1.374  -1.227  -1.181  -1.115
23    V         1.736   1.766   1.819   1.737   1.787   1.870   1.907   1.949   2.028
24    L         0.431   0.449   0.354   0.186   0.207   0.102   0.340   0.368   0.200
25    P         1.736   1.766   1.819   1.974   2.028   2.109   1.907   1.949   2.028
26    L         1.736   1.766   1.819   1.401   1.438   1.533   1.907   1.949   2.028
27    P         0.431   0.449   0.354   0.761   0.801   0.663   0.340   0.368   0.200
28    F        -0.677  -0.626  -0.660  -0.304  -0.207  -0.275  -0.705  -0.629  -0.659
29    R        -0.677  -0.729  -0.702  -0.423  -0.492  -0.458  -0.705  -0.762  -0.713
30    I         0.431   0.338   0.319   0.404   0.256   0.212   0.340   0.183   0.141
31    R         1.736   1.766   1.819   1.945   1.998   2.080   1.907   1.949   2.028
32    R        -0.677  -0.694  -0.691  -0.784  -0.809  -0.783  -0.705  -0.719  -0.699
33    G        -1.482  -1.452  -1.416  -1.216  -1.205  -1.102  -1.227  -1.181  -1.115
34    I         0.127   0.152   0.204  -0.379  -0.327  -0.243  -0.182  -0.142  -0.063
35    F        -1.482  -1.452  -1.416  -1.193  -1.129  -1.079  -1.227  -1.181  -1.115
36    S        -1.482  -1.452  -1.416  -1.499  -1.406  -1.387  -1.227  -1.181  -1.115
37    T        -0.677  -0.729  -0.702  -0.594  -0.681  -0.642  -0.705  -0.762  -0.713
38    Y        -0.677  -0.729  -0.702  -0.537  -0.627  -0.594  -0.705  -0.762  -0.713
39    N        -1.482  -1.452  -1.416  -1.189  -1.132  -1.075  -1.227  -1.181  -1.115
40    Q        -0.677  -0.729  -0.702  -0.594  -0.684  -0.636  -0.705  -0.762  -0.713
41    L         0.431   0.449   0.354   0.132   0.153   0.027   0.340   0.368   0.200
42    T        -0.677  -0.729  -0.702  -0.688  -0.763  -0.756  -0.705  -0.762  -0.713
43    A        -0.677  -0.694  -0.691  -0.658  -0.675  -0.664  -0.705  -0.719  -0.699
44    K        -1.482  -1.452  -1.416  -1.583  -1.574  -1.471  -1.227  -1.181  -1.115
45    Q        -1.482  -1.452  -1.416  -1.356  -1.330  -1.243  -1.227  -1.181  -1.115
46    Q         0.431   0.338   0.319   0.561   0.417   0.387   0.340   0.183   0.141
47    I         0.431   0.511   0.374   0.530   0.662   0.430   0.340   0.469   0.235
48    K         0.431   0.449   0.354   0.339   0.375   0.239   0.340   0.368   0.200
49    T         0.431   0.338   0.319   0.530   0.381   0.329   0.340   0.183   0.141
50    I        -0.677  -0.694  -0.691  -0.655  -0.660  -0.667  -0.705  -0.719  -0.699
51    I        -1.482  -1.452  -1.416  -1.651  -1.557  -1.540  -1.227  -1.181  -1.115
52    F        -1.482  -1.452  -1.416  -1.072  -0.997  -0.957  -1.227  -1.181  -1.115
53    I         0.431   0.338   0.319   0.443   0.304   0.270   0.340   0.183   0.141
54    T        -0.677  -0.607  -0.665  -0.727  -0.606  -0.725  -0.705  -0.604  -0.665
55    G        -1.482  -1.452  -1.416  -1.356  -1.322  -1.243  -1.227  -1.181  -1.115
56    C        -0.677  -0.676  -0.683  -0.322  -0.341  -0.344  -0.705  -0.698  -0.688
57    L         0.431   0.540   0.376   0.084   0.243  -0.001   0.340   0.515   0.239
58    V        -0.677  -0.676  -0.683  -0.898  -0.934  -0.936  -0.705  -0.698  -0.688
59    G        -0.677  -0.729  -0.702  -0.454  -0.553  -0.497  -0.705  -0.762  -0.713
60    L         0.431   0.338   0.319   0.186   0.056   0.056   0.340   0.183   0.141
61    L         0.431   0.338   0.319   0.124  -0.024  -0.036   0.340   0.183   0.141
62    F         1.736   1.766   1.819   1.857   1.905   1.991   1.907   1.949   2.028
63    I         0.431   0.338   0.319   0.438   0.296   0.263   0.340   0.183   0.141
64    D         1.736   1.766   1.819   1.952   1.999   2.087   1.907   1.949   2.028
65    S         0.431   0.338   0.319   0.520   0.404   0.391   0.340   0.183   0.141
66    W         0.431   0.338   0.319   0.802   0.642   0.588   0.340   0.183   0.141
67    K         0.431   0.338   0.319   0.301   0.162   0.132   0.340   0.183   0.141
68    R         1.736   1.766   1.819   1.945   1.998   2.080   1.907   1.949   2.028
69    S        -0.677  -0.694  -0.691  -0.808  -0.885  -0.873  -0.705  -0.719  -0.699
70    Q         0.431   0.540   0.376   0.561   0.725   0.482   0.340   0.515   0.239
71    I        -0.677  -0.729  -0.702  -0.808  -0.877  -0.865  -0.705  -0.762  -0.713
72    R        -0.677  -0.694  -0.691  -0.402  -0.428  -0.421  -0.705  -0.719  -0.699
73    V         0.431   0.338   0.319   0.489   0.357   0.330   0.340   0.183   0.141
74    S         0.259   0.329   0.286   0.201   0.334   0.233   0.050   0.169   0.083
75    L         0.431   0.449   0.354   0.132   0.153   0.027   0.340   0.368   0.200
76    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
77    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
78    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
79    Y         0.431   0.449   0.354   0.604   0.638   0.462   0.340   0.368   0.200
80    H         0.431   0.338   0.319   0.802   0.656   0.609   0.340   0.183   0.141
81    N        -0.677  -0.563  -0.650  -0.435  -0.268  -0.376  -0.705  -0.545  -0.646
82    D        -1.482  -1.452  -1.416  -1.486  -1.498  -1.374  -1.227  -1.181  -1.115
83    N         0.127   0.152   0.204  -0.119  -0.057   0.018  -0.182  -0.142  -0.063
84    S         0.431   0.449   0.354   0.467   0.502   0.366   0.340   0.368   0.200
85    G        -0.677  -0.563  -0.650  -0.475  -0.305  -0.418  -0.705  -0.545  -0.646
86    S        -0.677  -0.694  -0.691  -0.506  -0.526  -0.539  -0.705  -0.719  -0.699
87    I        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
88    G        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
89    S        -0.677  -0.676  -0.683  -0.791  -0.813  -0.805  -0.705  -0.698  -0.688
90    S        -0.814  -0.786  -0.745  -0.920  -0.858  -0.804  -0.879  -0.832  -0.766
91    A        -0.814  -0.786  -0.745  -1.016  -0.951  -0.901  -0.879  -0.832  -0.766
92    V        -0.814  -0.786  -0.745  -0.766  -0.704  -0.649  -0.879  -0.832  -0.766
93    T         1.736   1.766   1.819   1.836   1.889   1.970   1.907   1.949   2.028
94    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
95    P         0.431   0.511   0.374   0.467   0.590   0.397   0.340   0.469   0.235
96    I        -1.482  -1.452  -1.416  -1.530  -1.460  -1.418  -1.227  -1.181  -1.115
97    Q        -0.677  -0.729  -0.702  -0.629  -0.705  -0.678  -0.705  -0.762  -0.713
98    A        -1.482  -1.452  -1.416  -1.340  -1.281  -1.226  -1.227  -1.181  -1.115
99    L         1.736   1.766   1.819   1.401   1.438   1.533   1.907   1.949   2.028
100   A         0.431   0.449   0.354   0.559   0.587   0.438   0.340   0.368   0.200
101   S         0.127   0.152   0.204  -0.053  -0.011   0.069  -0.182  -0.142  -0.063
102   R         0.431   0.338   0.319   0.573   0.409   0.347   0.340   0.183   0.141
103   A         0.431   0.338   0.319   0.559   0.409   0.379   0.340   0.183   0.141
104   Y         0.431   0.540   0.376   0.651   0.823   0.565   0.340   0.515   0.239
105   N         0.431   0.338   0.319   0.554   0.401   0.349   0.340   0.183   0.141
106   Q         1.736   1.766   1.819   1.834   1.878   1.968   1.907   1.949   2.028
107   R         1.736   1.766   1.819   1.945   1.998   2.080   1.907   1.949   2.028
108   N         1.736   1.766   1.819   1.886   1.939   2.021   1.907   1.949   2.028
109   M        -0.677  -0.694  -0.691  -0.764  -0.840  -0.795  -0.705  -0.719  -0.699
110   Y         1.736   1.766   1.819   1.938   1.988   2.072   1.907   1.949   2.028
111   I         1.736   1.766   1.819   1.722   1.772   1.855   1.907   1.949   2.028
112   S         0.431   0.338   0.319   0.428   0.290   0.256   0.340   0.183   0.141
113   G         1.736   1.766   1.819   1.952   2.000   2.087   1.907   1.949   2.028
114   F         0.431   0.338   0.319   0.568   0.420   0.386   0.340   0.183   0.141
115   I         0.431   0.338   0.319   0.404   0.256   0.212   0.340   0.183   0.141
116   L         1.736   1.766   1.819   1.401   1.438   1.533   1.907   1.949   2.028
117   Y         1.736   1.766   1.819   1.938   1.988   2.072   1.907   1.949   2.028
118   F         0.431   0.338   0.319   0.431   0.245   0.181   0.340   0.183   0.141
119   S        -1.482  -1.452  -1.416  -1.495  -1.387  -1.383  -1.227  -1.181  -1.115
120   I         0.431   0.338   0.319   0.296   0.115   0.051   0.340   0.183   0.141
121   C        -0.677  -0.676  -0.683  -0.275  -0.288  -0.289  -0.705  -0.698  -0.688
122   I         1.736   1.766   1.819   1.722   1.772   1.855   1.907   1.949   2.028
123   P         0.431   0.338   0.319   0.711   0.576   0.537   0.340   0.183   0.141
124   T         0.431   0.449   0.354   0.535   0.573   0.400   0.340   0.368   0.200
125   V         0.431   0.449   0.354   0.311   0.341   0.139   0.340   0.368   0.200
126   M        -0.677  -0.694  -0.691  -0.926  -0.965  -0.907  -0.705  -0.719  -0.699
127   S        -0.677  -0.729  -0.702  -0.652  -0.716  -0.671  -0.705  -0.762  -0.713
128   I         0.431   0.449   0.354   0.296   0.326   0.125   0.340   0.368   0.200
129   V         0.127   0.161   0.211  -0.368  -0.340  -0.250  -0.182  -0.126  -0.049
130   K        -0.677  -0.607  -0.665  -0.731  -0.622  -0.689  -0.705  -0.604  -0.665
131   R         0.431   0.338   0.319   0.573   0.409   0.347   0.340   0.183   0.141
132   L         1.736   1.766   1.819   1.401   1.438   1.533   1.907   1.949   2.028
133   V         1.736   1.766   1.819   1.737   1.787   1.870   1.907   1.949   2.028
134   K         0.431   0.338   0.319   0.258   0.110   0.068   0.340   0.183   0.141
135   Y         0.431   0.338   0.319   0.566   0.400   0.340   0.340   0.183   0.141
136   Q         0.127   0.161   0.211  -0.003   0.053   0.136  -0.182  -0.126  -0.049
137   G        -1.482  -1.452  -1.416  -1.162  -1.124  -1.048  -1.227  -1.181  -1.115
138   L        -0.677  -0.676  -0.683  -0.842  -0.855  -0.803  -0.705  -0.698  -0.688
139   I        -0.677  -0.676  -0.683  -0.849  -0.823  -0.876  -0.705  -0.698  -0.688
140   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
141   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
142   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
143   N         0.431   0.338   0.319   0.554   0.401   0.349   0.340   0.183   0.141
144   E        -1.482  -1.452  -1.416  -1.324  -1.280  -1.210  -1.227  -1.181  -1.115
145   Q        -1.482  -1.452  -1.416  -1.465  -1.384  -1.352  -1.227  -1.181  -1.115
146   E        -0.677  -0.607  -0.665  -0.544  -0.427  -0.510  -0.705  -0.604  -0.665
147   K         0.431   0.449   0.354   0.304   0.336   0.191   0.340   0.368   0.200
148   Q        -0.677  -0.626  -0.660  -0.649  -0.562  -0.619  -0.705  -0.629  -0.659
149   K        -0.677  -0.694  -0.691  -0.662  -0.666  -0.688  -0.705  -0.719  -0.699
150   L        -1.482  -1.452  -1.416  -1.435  -1.375  -1.322  -1.227  -1.181  -1.115
151   N        -0.677  -0.694  -0.691  -0.825  -0.852  -0.831  -0.705  -0.719  -0.699
152   K         0.431   0.449   0.354   0.301   0.334   0.187   0.340   0.368   0.200
153   P        -1.482  -1.452  -1.416  -1.162  -1.132  -1.048  -1.227  -1.181  -1.115
154   S        -0.677  -0.694  -0.691  -0.658  -0.680  -0.684  -0.705  -0.719  -0.699
155   S        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
156   N        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
157   S        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
158   K        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
159   K         1.736   1.766   1.819   1.567   1.614   1.699   1.907   1.949   2.028
160   D         1.736   1.766   1.819   1.957   2.005   2.091   1.907   1.949   2.028
161   S        -0.814  -0.795  -0.745  -1.124  -1.097  -1.009  -0.879  -0.844  -0.766
162   N         0.259   0.319   0.264   0.165   0.277   0.141   0.050   0.150   0.044
163   E         0.259   0.283   0.256   0.006   0.049   0.001   0.050   0.089   0.030
164   A         0.127   0.150   0.203   0.114   0.152   0.244  -0.182  -0.146  -0.064
165   D        -0.677  -0.694  -0.691  -0.754  -0.776  -0.748  -0.705  -0.719  -0.699
166   S         0.127   0.161   0.211  -0.097  -0.028   0.051  -0.182  -0.126  -0.049
167   -        -0.814  -0.791  -0.745  -0.807  -0.753  -0.690  -0.879  -0.839  -0.766
168   -         0.259   0.327   0.295   0.084   0.214   0.129   0.050   0.164   0.098
169   -         0.259   0.225   0.256   0.132   0.073   0.115   0.050  -0.013   0.030
170   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
171   -         0.259   0.327   0.295   0.151   0.268   0.205   0.050   0.164   0.098
172   -         0.259   0.225   0.256   0.344   0.277   0.314   0.050  -0.013   0.030
173   -        -0.814  -0.791  -0.745  -0.881  -0.841  -0.765  -0.879  -0.839  -0.766
174   -        -0.814  -0.791  -0.745  -1.218  -1.168  -1.104  -0.879  -0.839  -0.766
175   T        -0.677  -0.676  -0.683  -0.506  -0.507  -0.504  -0.705  -0.698  -0.688
176   K        -0.677  -0.607  -0.665  -0.805  -0.692  -0.771  -0.705  -0.604  -0.665
177   L         0.431   0.338   0.319   0.160   0.026   0.018   0.340   0.183   0.141
178   Q        -0.677  -0.676  -0.683  -0.698  -0.721  -0.723  -0.705  -0.698  -0.688
179   E         0.127   0.161   0.211  -0.187  -0.139  -0.060  -0.182  -0.126  -0.049
180   E        -0.677  -0.676  -0.683  -0.781  -0.824  -0.812  -0.705  -0.698  -0.688
181   L         0.431   0.338   0.319   0.084  -0.071  -0.095   0.340   0.183   0.141
182   R        -0.677  -0.563  -0.650  -0.448  -0.280  -0.384  -0.705  -0.545  -0.646
183   K        -1.482  -1.452  -1.416  -1.534  -1.466  -1.422  -1.227  -1.181  -1.115
184   K         1.736   1.766   1.819   1.583   1.633   1.716   1.907   1.949   2.028
185   Q        -1.482  -1.452  -1.416  -1.448  -1.397  -1.335  -1.227  -1.181  -1.115
186   I        -0.677  -0.694  -0.691  -0.898  -0.964  -0.955  -0.705  -0.719  -0.699
187   S         0.431   0.338   0.319   0.460   0.327   0.303   0.340   0.183   0.141
188   L         0.431   0.338   0.319   0.084  -0.071  -0.095   0.340   0.183   0.141
189   E        -0.677  -0.729  -0.702  -0.711  -0.794  -0.767  -0.705  -0.762  -0.713
190   G        -0.677  -0.729  -0.702  -0.463  -0.548  -0.508  -0.705  -0.762  -0.713
191   L         1.736   1.766   1.819   1.401   1.438   1.533   1.907   1.949   2.028
192   Q         0.127   0.161   0.211  -0.158  -0.118  -0.035  -0.182  -0.126  -0.049
193   K        -0.677  -0.607  -0.665  -0.832  -0.717  -0.801  -0.705  -0.604  -0.665
194   Q         1.736   1.766   1.819   1.834   1.878   1.968   1.907   1.949   2.028
195   V         0.431   0.449   0.354   0.414   0.448   0.280   0.340   0.368   0.200
196   K        -1.482  -1.452  -1.416  -1.364  -1.279  -1.251  -1.227  -1.181  -1.115
197   N         0.431   0.338   0.319   0.632   0.498   0.465   0.340   0.183   0.141
198   L         1.736   1.766   1.819   1.401   1.438   1.533   1.907   1.949   2.028
199   E        -0.677  -0.729  -0.702  -0.585  -0.661  -0.614  -0.705  -0.762  -0.713
200   K         0.431   0.449   0.354   0.289   0.323   0.170   0.340   0.368   0.200
201   Y        -0.677  -0.729  -0.702  -0.429  -0.520  -0.463  -0.705  -0.762  -0.713
202   F         0.127   0.150   0.203   0.114   0.158   0.243  -0.182  -0.146  -0.064
203   D         1.736   1.766   1.819   1.952   1.999   2.087   1.907   1.949   2.028
204   E        -0.677  -0.729  -0.702  -0.553  -0.621  -0.576  -0.705  -0.762  -0.713
205   K        -1.482  -1.452  -1.416  -1.565  -1.565  -1.453  -1.227  -1.181  -1.115
206   N         0.431   0.449   0.354   0.559   0.596   0.421   0.340   0.368   0.200
207   Q         0.259   0.283   0.256   0.025   0.065   0.024   0.050   0.089   0.030
208   P         0.431   0.449   0.354   0.716   0.753   0.601   0.340   0.368   0.200
209   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
210   G        -0.814  -0.795  -0.745  -0.802  -0.780  -0.685  -0.879  -0.844  -0.766
211   N        -0.814  -0.795  -0.745  -0.790  -0.746  -0.673  -0.879  -0.844  -0.766
212   V        -0.814  -0.795  -0.745  -0.962  -0.910  -0.846  -0.879  -0.844  -0.766
213   A        -0.814  -0.795  -0.745  -0.790  -0.753  -0.673  -0.879  -0.844  -0.766
214   A        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
215   A        -0.677  -0.694  -0.691  -0.506  -0.547  -0.544  -0.705  -0.719  -0.699
216   E        -0.677  -0.729  -0.702  -0.604  -0.686  -0.637  -0.705  -0.762  -0.713
217   A        -0.677  -0.694  -0.691  -0.724  -0.756  -0.741  -0.705  -0.719  -0.699
218   S        -0.677  -0.694  -0.691  -0.711  -0.740  -0.747  -0.705  -0.719  -0.699
219   K         0.431   0.338   0.319   0.342   0.214   0.191   0.340   0.183   0.141
220   K         1.736   1.766   1.819   1.583   1.633   1.716   1.907   1.949   2.028
221   G        -1.482  -1.452  -1.416  -1.201  -1.132  -1.086  -1.227  -1.181  -1.115
222   N        -1.482  -1.452  -1.416  -1.232  -1.185  -1.118  -1.227  -1.181  -1.115
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P35723
Output conservation file - output/P35723
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
