1     M         0.523   0.544   0.572   0.892   0.839   0.832   0.528   0.560   0.595
2     S        -1.842  -1.457  -1.160  -2.174  -1.643  -1.166  -2.438  -1.865  -1.388
3     A        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
4     P        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
5     A        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
6     Q        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
7     N        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
8     N        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
9     A        -1.842  -1.453  -1.158  -2.398  -1.747  -1.265  -2.438  -1.858  -1.386
10    E        -1.831  -1.947  -2.159  -1.628  -1.843  -2.161  -1.696  -1.853  -2.035
11    V        -0.678  -0.837  -1.137  -0.795  -0.960  -1.388  -0.658  -0.900  -1.352
12    P        -0.678  -1.060  -1.143  -0.456  -1.045  -1.254  -0.658  -1.229  -1.362
13    T        -0.678  -1.060  -1.143  -0.559  -1.135  -1.308  -0.658  -1.229  -1.362
14    F         0.523   0.544   0.572   0.548   0.577   0.614   0.528   0.560   0.595
15    K         0.523   0.544   0.572   0.271   0.369   0.438   0.528   0.560   0.595
16    L         0.523   0.544   0.572   0.287   0.382   0.448   0.528   0.560   0.595
17    V         0.523   0.544   0.572   0.250   0.343   0.424   0.528   0.560   0.595
18    L         0.523   0.544   0.572   0.287   0.382   0.448   0.528   0.560   0.595
19    V         0.523   0.544   0.572   0.250   0.343   0.424   0.528   0.560   0.595
20    G         0.523   0.544   0.572   0.413   0.462   0.528   0.528   0.560   0.595
21    D         0.523   0.544   0.572   0.460   0.502   0.558   0.528   0.560   0.595
22    G         0.523   0.544   0.572   0.413   0.462   0.528   0.528   0.560   0.595
23    G         0.523   0.544   0.572   0.413   0.462   0.528   0.528   0.560   0.595
24    T         0.523   0.544   0.572   0.497   0.532   0.581   0.528   0.560   0.595
25    G         0.523   0.544   0.572   0.413   0.462   0.528   0.528   0.560   0.595
26    K         0.523   0.544   0.572   0.271   0.369   0.438   0.528   0.560   0.595
27    T         0.523   0.544   0.572   0.497   0.532   0.581   0.528   0.560   0.595
28    T         0.523   0.544   0.572   0.497   0.532   0.581   0.528   0.560   0.595
29    F         0.523   0.544   0.572   0.548   0.577   0.614   0.528   0.560   0.595
30    V         0.523   0.544   0.572   0.250   0.343   0.424   0.528   0.560   0.595
31    K         0.523   0.544   0.572   0.271   0.369   0.438   0.528   0.560   0.595
32    R         0.523   0.544   0.572   0.702   0.682   0.711   0.528   0.560   0.595
33    H         0.523   0.544   0.572   0.878   0.840   0.822   0.528   0.560   0.595
34    L        -0.678  -1.060  -1.143  -0.763  -1.263  -1.397  -0.658  -1.229  -1.362
35    T         0.523   0.544   0.572   0.497   0.532   0.581   0.528   0.560   0.595
36    G         0.523   0.544   0.572   0.413   0.462   0.528   0.528   0.560   0.595
37    E         0.523   0.544   0.572   0.450   0.501   0.551   0.528   0.560   0.595
38    F         0.523   0.544   0.572   0.548   0.577   0.614   0.528   0.560   0.595
39    E        -0.678  -1.060  -1.143  -0.625  -1.195  -1.395  -0.658  -1.229  -1.362
40    K        -0.678  -1.060  -1.143  -0.760  -1.246  -1.342  -0.658  -1.229  -1.362
41    K        -0.678  -1.060  -1.143  -0.747  -1.217  -1.300  -0.658  -1.229  -1.362
42    Y         0.523   0.544   0.572   0.717   0.719   0.720   0.528   0.560   0.595
43    I        -1.831  -2.274  -2.166  -1.378  -2.083  -1.916  -1.696  -2.254  -2.042
44    A         0.523   0.544   0.572   0.403   0.440   0.521   0.528   0.560   0.595
45    T         0.523   0.544   0.572   0.497   0.532   0.581   0.528   0.560   0.595
46    I        -1.842  -1.172  -1.136  -2.504  -1.390  -1.312  -2.438  -1.401  -1.350
47    G         0.523   0.544   0.572   0.413   0.462   0.528   0.528   0.560   0.595
48    V         0.523   0.544   0.572   0.250   0.343   0.424   0.528   0.560   0.595
49    E         0.523   0.544   0.572   0.450   0.501   0.551   0.528   0.560   0.595
50    V         0.523   0.544   0.572   0.250   0.343   0.424   0.528   0.560   0.595
51    H         0.523   0.544   0.572   0.878   0.840   0.822   0.528   0.560   0.595
52    P         0.523   0.544   0.572   0.600   0.624   0.646   0.528   0.560   0.595
53    L         0.523   0.544   0.572   0.287   0.382   0.448   0.528   0.560   0.595
54    S        -4.521  -3.837  -3.445  -4.486  -3.599  -2.863  -3.921  -3.154  -2.576
55    F         0.523   0.544   0.572   0.548   0.577   0.614   0.528   0.560   0.595
56    Y        -3.660  -3.371  -2.887  -3.334  -3.045  -2.385  -3.476  -3.003  -2.377
57    T         0.523   0.544   0.572   0.497   0.532   0.581   0.528   0.560   0.595
58    N        -0.678  -1.060  -1.143  -0.449  -1.057  -1.231  -0.658  -1.229  -1.362
59    F        -0.678  -1.060  -1.143  -0.439  -0.953  -1.062  -0.658  -1.229  -1.362
60    G         0.523   0.544   0.572   0.413   0.462   0.528   0.528   0.560   0.595
61    E        -1.831  -2.274  -2.166  -1.592  -2.260  -2.079  -1.696  -2.254  -2.042
62    I        -1.301  -0.660  -1.128  -1.352  -0.444  -1.251  -1.548  -0.654  -1.338
63    K        -2.929  -3.215  -2.889  -2.691  -3.089  -2.504  -2.586  -2.861  -2.378
64    F         0.523   0.544   0.572   0.548   0.577   0.614   0.528   0.560   0.595
65    D        -0.678  -0.660  -1.133  -0.571  -0.554  -1.230  -0.658  -0.654  -1.346
66    V         0.523   0.544   0.572   0.250   0.343   0.424   0.528   0.560   0.595
67    W         0.523   0.544   0.572   0.947   0.891   0.867   0.528   0.560   0.595
68    D         0.523   0.544   0.572   0.460   0.502   0.558   0.528   0.560   0.595
69    T         0.523   0.544   0.572   0.497   0.532   0.581   0.528   0.560   0.595
70    A         0.523   0.544   0.572   0.403   0.440   0.521   0.528   0.560   0.595
71    G         0.523   0.544   0.572   0.413   0.462   0.528   0.528   0.560   0.595
72    Q         0.523   0.544   0.572   0.651   0.688   0.679   0.528   0.560   0.595
73    E         0.523   0.544   0.572   0.450   0.501   0.551   0.528   0.560   0.595
74    K         0.523   0.544   0.572   0.271   0.369   0.438   0.528   0.560   0.595
75    F        -0.678  -0.660  -1.133  -0.521  -0.515  -1.306  -0.658  -0.654  -1.346
76    G         0.523   0.544   0.572   0.413   0.462   0.528   0.528   0.560   0.595
77    G         0.523   0.544   0.572   0.413   0.462   0.528   0.528   0.560   0.595
78    L         0.523   0.544   0.572   0.287   0.382   0.448   0.528   0.560   0.595
79    R         0.523   0.544   0.572   0.702   0.682   0.711   0.528   0.560   0.595
80    D         0.523   0.544   0.572   0.460   0.502   0.558   0.528   0.560   0.595
81    G         0.523   0.544   0.572   0.413   0.462   0.528   0.528   0.560   0.595
82    Y         0.523   0.544   0.572   0.717   0.719   0.720   0.528   0.560   0.595
83    Y         0.523   0.544   0.572   0.717   0.719   0.720   0.528   0.560   0.595
84    I         0.523   0.544   0.572   0.630   0.647   0.666   0.528   0.560   0.595
85    N        -0.678  -0.660  -1.133  -0.425  -0.448  -1.138  -0.658  -0.654  -1.346
86    A        -1.842  -1.172  -1.136  -2.551  -1.392  -1.337  -2.438  -1.401  -1.350
87    Q         0.523   0.544   0.572   0.651   0.688   0.679   0.528   0.560   0.595
88    C         0.523   0.544   0.572   0.942   0.883   0.863   0.528   0.560   0.595
89    A        -0.678  -0.263  -1.127  -0.642  -0.156  -1.320  -0.658  -0.157  -1.336
90    I         0.523   0.544   0.572   0.630   0.647   0.666   0.528   0.560   0.595
91    I         0.523   0.544   0.572   0.630   0.647   0.666   0.528   0.560   0.595
92    M         0.523   0.544   0.572   0.892   0.839   0.832   0.528   0.560   0.595
93    F         0.523   0.544   0.572   0.548   0.577   0.614   0.528   0.560   0.595
94    D         0.523   0.544   0.572   0.460   0.502   0.558   0.528   0.560   0.595
95    V         0.523   0.544   0.572   0.250   0.343   0.424   0.528   0.560   0.595
96    T         0.523   0.544   0.572   0.497   0.532   0.581   0.528   0.560   0.595
97    S         0.523   0.544   0.572   0.878   0.840   0.822   0.528   0.560   0.595
98    R         0.523   0.544   0.572   0.702   0.682   0.711   0.528   0.560   0.595
99    I         0.523   0.544   0.572   0.630   0.647   0.666   0.528   0.560   0.595
100   T         0.523   0.544   0.572   0.497   0.532   0.581   0.528   0.560   0.595
101   Y         0.523   0.544   0.572   0.717   0.719   0.720   0.528   0.560   0.595
102   K         0.523   0.544   0.572   0.271   0.369   0.438   0.528   0.560   0.595
103   N         0.523   0.544   0.572   0.600   0.592   0.646   0.528   0.560   0.595
104   V         0.523   0.544   0.572   0.250   0.343   0.424   0.528   0.560   0.595
105   P         0.523   0.544   0.572   0.600   0.624   0.646   0.528   0.560   0.595
106   N        -0.678  -0.660  -1.133  -0.396  -0.421  -1.050  -0.658  -0.654  -1.346
107   W         0.523   0.544   0.572   0.947   0.891   0.867   0.528   0.560   0.595
108   H        -0.678  -0.660  -1.133  -0.112  -0.169  -0.883  -0.658  -0.654  -1.346
109   R         0.523   0.544   0.572   0.702   0.682   0.711   0.528   0.560   0.595
110   D         0.523   0.544   0.572   0.460   0.502   0.558   0.528   0.560   0.595
111   L         0.523   0.544   0.572   0.287   0.382   0.448   0.528   0.560   0.595
112   V        -0.678  -0.837  -1.137  -0.795  -0.960  -1.388  -0.658  -0.900  -1.352
113   R         0.523   0.544   0.572   0.702   0.682   0.711   0.528   0.560   0.595
114   V         0.523   0.544   0.572   0.250   0.343   0.424   0.528   0.560   0.595
115   C         0.523   0.544   0.572   0.942   0.883   0.863   0.528   0.560   0.595
116   E         0.523   0.544   0.572   0.450   0.501   0.551   0.528   0.560   0.595
117   N         0.523   0.544   0.572   0.600   0.592   0.646   0.528   0.560   0.595
118   I         0.523   0.544   0.572   0.630   0.647   0.666   0.528   0.560   0.595
119   P         0.523   0.544   0.572   0.600   0.624   0.646   0.528   0.560   0.595
120   I         0.523   0.544   0.572   0.630   0.647   0.666   0.528   0.560   0.595
121   V         0.523   0.544   0.572   0.250   0.343   0.424   0.528   0.560   0.595
122   L         0.523   0.544   0.572   0.287   0.382   0.448   0.528   0.560   0.595
123   C         0.523   0.544   0.572   0.942   0.883   0.863   0.528   0.560   0.595
124   G         0.523   0.544   0.572   0.413   0.462   0.528   0.528   0.560   0.595
125   N         0.523   0.544   0.572   0.600   0.592   0.646   0.528   0.560   0.595
126   K         0.523   0.544   0.572   0.271   0.369   0.438   0.528   0.560   0.595
127   V         0.523   0.544   0.572   0.250   0.343   0.424   0.528   0.560   0.595
128   D         0.523   0.544   0.572   0.460   0.502   0.558   0.528   0.560   0.595
129   V         0.523   0.544   0.572   0.250   0.343   0.424   0.528   0.560   0.595
130   K         0.523   0.544   0.572   0.271   0.369   0.438   0.528   0.560   0.595
131   E         0.523   0.544   0.572   0.450   0.501   0.551   0.528   0.560   0.595
132   R         0.523   0.544   0.572   0.702   0.682   0.711   0.528   0.560   0.595
133   K         0.523   0.544   0.572   0.271   0.369   0.438   0.528   0.560   0.595
134   V         0.523   0.544   0.572   0.250   0.343   0.424   0.528   0.560   0.595
135   K         0.523   0.544   0.572   0.271   0.369   0.438   0.528   0.560   0.595
136   A         0.523   0.544   0.572   0.403   0.440   0.521   0.528   0.560   0.595
137   K         0.523   0.544   0.572   0.271   0.369   0.438   0.528   0.560   0.595
138   T        -0.678  -0.660  -1.133  -0.559  -0.550  -1.289  -0.658  -0.654  -1.346
139   I         0.523   0.544   0.572   0.630   0.647   0.666   0.528   0.560   0.595
140   T         0.523   0.544   0.572   0.497   0.532   0.581   0.528   0.560   0.595
141   F         0.523   0.544   0.572   0.548   0.577   0.614   0.528   0.560   0.595
142   H         0.523   0.544   0.572   0.878   0.840   0.822   0.528   0.560   0.595
143   R         0.523   0.544   0.572   0.702   0.682   0.711   0.528   0.560   0.595
144   K         0.523   0.544   0.572   0.271   0.369   0.438   0.528   0.560   0.595
145   K         0.523   0.544   0.572   0.271   0.369   0.438   0.528   0.560   0.595
146   N         0.523   0.544   0.572   0.600   0.592   0.646   0.528   0.560   0.595
147   L         0.523   0.544   0.572   0.287   0.382   0.448   0.528   0.560   0.595
148   Q         0.523   0.544   0.572   0.651   0.688   0.679   0.528   0.560   0.595
149   Y         0.523   0.544   0.572   0.717   0.719   0.720   0.528   0.560   0.595
150   Y         0.523   0.544   0.572   0.717   0.719   0.720   0.528   0.560   0.595
151   D         0.523   0.544   0.572   0.460   0.502   0.558   0.528   0.560   0.595
152   I         0.523   0.544   0.572   0.630   0.647   0.666   0.528   0.560   0.595
153   S         0.523   0.544   0.572   0.878   0.840   0.822   0.528   0.560   0.595
154   A         0.523   0.544   0.572   0.403   0.440   0.521   0.528   0.560   0.595
155   K         0.523   0.544   0.572   0.271   0.369   0.438   0.528   0.560   0.595
156   S         0.523   0.544   0.572   0.878   0.840   0.822   0.528   0.560   0.595
157   N         0.523   0.544   0.572   0.600   0.592   0.646   0.528   0.560   0.595
158   Y         0.523   0.544   0.572   0.717   0.719   0.720   0.528   0.560   0.595
159   N         0.523   0.544   0.572   0.600   0.592   0.646   0.528   0.560   0.595
160   F         0.523   0.544   0.572   0.548   0.577   0.614   0.528   0.560   0.595
161   E         0.523   0.544   0.572   0.450   0.501   0.551   0.528   0.560   0.595
162   K         0.523   0.544   0.572   0.271   0.369   0.438   0.528   0.560   0.595
163   P         0.523   0.544   0.572   0.600   0.624   0.646   0.528   0.560   0.595
164   F         0.523   0.544   0.572   0.548   0.577   0.614   0.528   0.560   0.595
165   L         0.523   0.544   0.572   0.287   0.382   0.448   0.528   0.560   0.595
166   W         0.523   0.544   0.572   0.947   0.891   0.867   0.528   0.560   0.595
167   L         0.523   0.544   0.572   0.287   0.382   0.448   0.528   0.560   0.595
168   A         0.523   0.544   0.572   0.403   0.440   0.521   0.528   0.560   0.595
169   R         0.523   0.544   0.572   0.702   0.682   0.711   0.528   0.560   0.595
170   K         0.523   0.544   0.572   0.271   0.369   0.438   0.528   0.560   0.595
171   L         0.523   0.544   0.572   0.287   0.382   0.448   0.528   0.560   0.595
172   A        -1.842  -1.457  -1.160  -2.660  -2.020  -1.460  -2.438  -1.865  -1.388
173   G         0.523   0.544   0.572   0.413   0.462   0.528   0.528   0.560   0.595
174   N         0.523   0.544   0.572   0.600   0.592   0.646   0.528   0.560   0.595
175   P        -0.678  -0.837  -1.137  -0.432  -0.668  -1.165  -0.658  -0.900  -1.352
176   Q        -2.929  -3.215  -2.889  -2.387  -2.925  -2.508  -2.586  -2.861  -2.378
177   L         0.523   0.544   0.572   0.287   0.382   0.448   0.528   0.560   0.595
178   E        -1.301  -0.605  -1.125  -1.482  -0.500  -1.276  -1.548  -0.581  -1.334
179   F         0.523   0.544   0.572   0.548   0.577   0.614   0.528   0.560   0.595
180   V         0.523   0.544   0.572   0.250   0.343   0.424   0.528   0.560   0.595
181   A         0.523   0.544   0.572   0.403   0.440   0.521   0.528   0.560   0.595
182   S        -1.831  -2.274  -2.166  -1.216  -2.046  -2.023  -1.696  -2.254  -2.042
183   P         0.523   0.544   0.572   0.600   0.624   0.646   0.528   0.560   0.595
184   A         0.523   0.544   0.572   0.403   0.440   0.521   0.528   0.560   0.595
185   L         0.523   0.544   0.572   0.287   0.382   0.448   0.528   0.560   0.595
186   A        -0.678  -1.060  -1.143  -0.615  -1.149  -1.233  -0.658  -1.229  -1.362
187   P         0.523   0.544   0.572   0.600   0.624   0.646   0.528   0.560   0.595
188   P        -0.678  -1.060  -1.143  -0.456  -1.045  -1.254  -0.658  -1.229  -1.362
189   E        -0.678  -0.837  -1.137  -0.594  -0.804  -1.283  -0.658  -0.900  -1.352
190   V        -1.831  -2.274  -2.166  -1.844  -2.506  -2.297  -1.696  -2.254  -2.042
191   Q        -1.831  -2.274  -2.166  -1.476  -2.248  -2.212  -1.696  -2.254  -2.042
192   V        -0.678  -1.060  -1.143  -0.777  -1.253  -1.340  -0.658  -1.229  -1.362
193   D         0.523   0.544   0.572   0.460   0.502   0.558   0.528   0.560   0.595
194   E        -3.860  -3.352  -2.887  -4.055  -3.307  -2.646  -3.624  -2.998  -2.376
195   Q        -2.903  -2.371  -2.165  -3.073  -2.451  -2.124  -3.031  -2.426  -2.042
196   L        -0.678  -1.060  -1.143  -0.789  -1.313  -1.481  -0.658  -1.229  -1.362
197   M        -0.678  -0.660  -1.133  -0.177  -0.253  -1.123  -0.658  -0.654  -1.346
198   H        -2.929  -2.656  -2.881  -2.411  -2.220  -2.543  -2.586  -2.312  -2.372
199   Q        -3.433  -3.068  -2.886  -3.307  -3.034  -2.680  -3.179  -2.784  -2.376
200   Y        -0.678  -1.060  -1.143  -0.278  -0.832  -0.999  -0.658  -1.229  -1.362
201   Q        -0.678  -0.837  -1.137  -0.368  -0.586  -1.099  -0.658  -0.900  -1.352
202   Q        -1.831  -2.274  -2.166  -1.473  -2.247  -2.204  -1.696  -2.254  -2.042
203   E        -0.678  -1.060  -1.143  -0.599  -1.146  -1.309  -0.658  -1.229  -1.362
204   M        -0.678  -1.060  -1.143  -0.177  -0.854  -1.148  -0.658  -1.229  -1.362
205   D        -2.399  -2.187  -2.164  -2.463  -2.219  -2.150  -2.438  -2.169  -2.040
206   Q        -1.668  -1.429  -1.148  -1.954  -1.689  -1.238  -2.141  -1.818  -1.369
207   A         0.523   0.544   0.572   0.403   0.440   0.521   0.528   0.560   0.595
208   T        -2.399  -2.559  -2.168  -2.436  -2.810  -2.159  -2.438  -2.599  -2.045
209   A        -0.678  -0.837  -1.137  -0.622  -0.829  -1.246  -0.658  -0.900  -1.352
210   L        -1.668  -1.429  -1.148  -2.079  -1.594  -1.162  -2.141  -1.818  -1.369
211   P         0.523   0.544   0.572   0.600   0.624   0.646   0.528   0.560   0.595
212   L         0.523   0.544   0.572   0.287   0.382   0.448   0.528   0.560   0.595
213   P         0.523   0.544   0.572   0.600   0.624   0.646   0.528   0.560   0.595
214   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
215   D        -0.678  -0.837  -1.137  -0.590  -0.809  -1.297  -0.658  -0.900  -1.352
216   E         0.523   0.544   0.572   0.450   0.501   0.551   0.528   0.560   0.595
217   D         0.523   0.544   0.572   0.460   0.502   0.558   0.528   0.560   0.595
218   D         0.523   0.544   0.572   0.460   0.502   0.558   0.528   0.560   0.595
219   A        -0.678  -0.837  -1.137  -0.646  -0.871  -1.322  -0.658  -0.900  -1.352
220   D         0.523   0.544   0.572   0.460   0.502   0.558   0.528   0.560   0.595
221   L        -0.678  -1.060  -1.143  -0.751  -1.238  -1.356  -0.658  -1.229  -1.362
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P32836
Output conservation file - output/P32836
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
