1     M         0.525   0.545   0.573   0.899   0.841   0.834   0.529   0.561   0.596
2     S        -1.884  -1.473  -1.173  -2.218  -1.659  -1.173  -2.486  -1.878  -1.394
3     A        -1.884  -1.467  -1.171  -2.449  -1.759  -1.274  -2.486  -1.868  -1.391
4     P        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
5     A        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
6     A        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
7     N        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
8     G        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
9     E        -1.873  -1.988  -2.182  -1.668  -1.891  -2.175  -1.732  -1.891  -2.044
10    V        -0.698  -0.865  -1.151  -0.816  -0.993  -1.397  -0.677  -0.933  -1.360
11    P        -0.698  -1.071  -1.157  -0.472  -1.053  -1.262  -0.677  -1.236  -1.368
12    T        -0.698  -1.071  -1.157  -0.577  -1.144  -1.316  -0.677  -1.236  -1.368
13    F         0.525   0.545   0.573   0.549   0.578   0.614   0.529   0.561   0.596
14    K         0.525   0.545   0.573   0.267   0.369   0.438   0.529   0.561   0.596
15    L         0.525   0.545   0.573   0.283   0.381   0.448   0.529   0.561   0.596
16    V         0.525   0.545   0.573   0.246   0.343   0.424   0.529   0.561   0.596
17    L         0.525   0.545   0.573   0.283   0.381   0.448   0.529   0.561   0.596
18    V         0.525   0.545   0.573   0.246   0.343   0.424   0.529   0.561   0.596
19    G         0.525   0.545   0.573   0.412   0.461   0.528   0.529   0.561   0.596
20    D         0.525   0.545   0.573   0.465   0.505   0.562   0.529   0.561   0.596
21    G         0.525   0.545   0.573   0.412   0.461   0.528   0.529   0.561   0.596
22    G         0.525   0.545   0.573   0.412   0.461   0.528   0.529   0.561   0.596
23    T         0.525   0.545   0.573   0.496   0.532   0.581   0.529   0.561   0.596
24    G         0.525   0.545   0.573   0.412   0.461   0.528   0.529   0.561   0.596
25    K         0.525   0.545   0.573   0.267   0.369   0.438   0.529   0.561   0.596
26    T         0.525   0.545   0.573   0.496   0.532   0.581   0.529   0.561   0.596
27    T         0.525   0.545   0.573   0.496   0.532   0.581   0.529   0.561   0.596
28    F         0.525   0.545   0.573   0.549   0.578   0.614   0.529   0.561   0.596
29    V         0.525   0.545   0.573   0.246   0.343   0.424   0.529   0.561   0.596
30    K         0.525   0.545   0.573   0.267   0.369   0.438   0.529   0.561   0.596
31    R         0.525   0.545   0.573   0.705   0.683   0.712   0.529   0.561   0.596
32    H         0.525   0.545   0.573   0.889   0.845   0.827   0.529   0.561   0.596
33    L        -0.698  -1.071  -1.157  -0.785  -1.273  -1.407  -0.677  -1.236  -1.368
34    T         0.525   0.545   0.573   0.496   0.532   0.581   0.529   0.561   0.596
35    G         0.525   0.545   0.573   0.412   0.461   0.528   0.529   0.561   0.596
36    E         0.525   0.545   0.573   0.444   0.499   0.548   0.529   0.561   0.596
37    F         0.525   0.545   0.573   0.549   0.578   0.614   0.529   0.561   0.596
38    E        -0.698  -1.071  -1.157  -0.649  -1.206  -1.406  -0.677  -1.236  -1.368
39    K        -0.698  -1.071  -1.157  -0.781  -1.255  -1.350  -0.677  -1.236  -1.368
40    K        -0.698  -1.071  -1.157  -0.767  -1.226  -1.307  -0.677  -1.236  -1.368
41    Y         0.525   0.545   0.573   0.720   0.721   0.722   0.529   0.561   0.596
42    I        -1.873  -2.309  -2.188  -1.409  -2.115  -1.926  -1.732  -2.283  -2.051
43    A         0.525   0.545   0.573   0.401   0.439   0.522   0.529   0.561   0.596
44    T         0.525   0.545   0.573   0.496   0.532   0.581   0.529   0.561   0.596
45    I        -1.884  -1.168  -1.147  -2.553  -1.375  -1.316  -2.486  -1.386  -1.354
46    G         0.525   0.545   0.573   0.412   0.461   0.528   0.529   0.561   0.596
47    V         0.525   0.545   0.573   0.246   0.343   0.424   0.529   0.561   0.596
48    E         0.525   0.545   0.573   0.444   0.499   0.548   0.529   0.561   0.596
49    V         0.525   0.545   0.573   0.246   0.343   0.424   0.529   0.561   0.596
50    H         0.525   0.545   0.573   0.889   0.845   0.827   0.529   0.561   0.596
51    P         0.525   0.545   0.573   0.601   0.625   0.647   0.529   0.561   0.596
52    L         0.525   0.545   0.573   0.283   0.381   0.448   0.529   0.561   0.596
53    S        -4.612  -3.851  -3.478  -4.568  -3.603  -2.875  -3.993  -3.159  -2.586
54    F         0.525   0.545   0.573   0.549   0.578   0.614   0.529   0.561   0.596
55    Y        -3.735  -3.401  -2.915  -3.393  -3.058  -2.394  -3.541  -3.019  -2.386
56    T         0.525   0.545   0.573   0.496   0.532   0.581   0.529   0.561   0.596
57    N        -0.698  -1.071  -1.157  -0.464  -1.064  -1.237  -0.677  -1.236  -1.368
58    F        -0.698  -1.071  -1.157  -0.455  -0.960  -1.069  -0.677  -1.236  -1.368
59    G         0.525   0.545   0.573   0.412   0.461   0.528   0.529   0.561   0.596
60    E        -1.873  -2.309  -2.188  -1.633  -2.298  -2.093  -1.732  -2.283  -2.051
61    I        -1.333  -0.668  -1.142  -1.383  -0.448  -1.259  -1.581  -0.658  -1.345
62    K        -2.991  -3.258  -2.918  -2.745  -3.127  -2.517  -2.637  -2.890  -2.388
63    F         0.525   0.545   0.573   0.549   0.578   0.614   0.529   0.561   0.596
64    D        -0.698  -0.676  -1.147  -0.583  -0.566  -1.235  -0.677  -0.669  -1.353
65    V         0.525   0.545   0.573   0.246   0.343   0.424   0.529   0.561   0.596
66    W         0.525   0.545   0.573   0.954   0.894   0.868   0.529   0.561   0.596
67    D         0.525   0.545   0.573   0.465   0.505   0.562   0.529   0.561   0.596
68    T         0.525   0.545   0.573   0.496   0.532   0.581   0.529   0.561   0.596
69    A         0.525   0.545   0.573   0.401   0.439   0.522   0.529   0.561   0.596
70    G         0.525   0.545   0.573   0.412   0.461   0.528   0.529   0.561   0.596
71    Q         0.525   0.545   0.573   0.648   0.687   0.676   0.529   0.561   0.596
72    E         0.525   0.545   0.573   0.444   0.499   0.548   0.529   0.561   0.596
73    K         0.525   0.545   0.573   0.267   0.369   0.438   0.529   0.561   0.596
74    F        -0.698  -0.676  -1.147  -0.538  -0.529  -1.315  -0.677  -0.669  -1.353
75    G         0.525   0.545   0.573   0.412   0.461   0.528   0.529   0.561   0.596
76    G         0.525   0.545   0.573   0.412   0.461   0.528   0.529   0.561   0.596
77    L         0.525   0.545   0.573   0.283   0.381   0.448   0.529   0.561   0.596
78    R         0.525   0.545   0.573   0.705   0.683   0.712   0.529   0.561   0.596
79    D         0.525   0.545   0.573   0.465   0.505   0.562   0.529   0.561   0.596
80    G         0.525   0.545   0.573   0.412   0.461   0.528   0.529   0.561   0.596
81    Y         0.525   0.545   0.573   0.720   0.721   0.722   0.529   0.561   0.596
82    Y         0.525   0.545   0.573   0.720   0.721   0.722   0.529   0.561   0.596
83    I         0.525   0.545   0.573   0.632   0.649   0.667   0.529   0.561   0.596
84    N        -0.698  -0.676  -1.147  -0.441  -0.462  -1.148  -0.677  -0.669  -1.353
85    A        -1.884  -1.168  -1.147  -2.602  -1.375  -1.341  -2.486  -1.386  -1.354
86    Q         0.525   0.545   0.573   0.648   0.687   0.676   0.529   0.561   0.596
87    C         0.525   0.545   0.573   0.949   0.885   0.865   0.529   0.561   0.596
88    A        -0.698  -0.229  -1.140  -0.661  -0.118  -1.327  -0.677  -0.113  -1.342
89    I         0.525   0.545   0.573   0.632   0.649   0.667   0.529   0.561   0.596
90    I         0.525   0.545   0.573   0.632   0.649   0.667   0.529   0.561   0.596
91    M         0.525   0.545   0.573   0.899   0.841   0.834   0.529   0.561   0.596
92    F         0.525   0.545   0.573   0.549   0.578   0.614   0.529   0.561   0.596
93    D         0.525   0.545   0.573   0.465   0.505   0.562   0.529   0.561   0.596
94    V         0.525   0.545   0.573   0.246   0.343   0.424   0.529   0.561   0.596
95    T         0.525   0.545   0.573   0.496   0.532   0.581   0.529   0.561   0.596
96    S         0.525   0.545   0.573   0.884   0.842   0.824   0.529   0.561   0.596
97    R         0.525   0.545   0.573   0.705   0.683   0.712   0.529   0.561   0.596
98    I         0.525   0.545   0.573   0.632   0.649   0.667   0.529   0.561   0.596
99    T         0.525   0.545   0.573   0.496   0.532   0.581   0.529   0.561   0.596
100   Y         0.525   0.545   0.573   0.720   0.721   0.722   0.529   0.561   0.596
101   K         0.525   0.545   0.573   0.267   0.369   0.438   0.529   0.561   0.596
102   N         0.525   0.545   0.573   0.601   0.593   0.647   0.529   0.561   0.596
103   V         0.525   0.545   0.573   0.246   0.343   0.424   0.529   0.561   0.596
104   P         0.525   0.545   0.573   0.601   0.625   0.647   0.529   0.561   0.596
105   N        -0.698  -0.676  -1.147  -0.411  -0.435  -1.055  -0.677  -0.669  -1.353
106   W         0.525   0.545   0.573   0.954   0.894   0.868   0.529   0.561   0.596
107   H        -0.698  -0.676  -1.147  -0.117  -0.178  -0.887  -0.677  -0.669  -1.353
108   R         0.525   0.545   0.573   0.705   0.683   0.712   0.529   0.561   0.596
109   D         0.525   0.545   0.573   0.465   0.505   0.562   0.529   0.561   0.596
110   L         0.525   0.545   0.573   0.283   0.381   0.448   0.529   0.561   0.596
111   V        -0.698  -0.865  -1.151  -0.816  -0.993  -1.397  -0.677  -0.933  -1.360
112   R         0.525   0.545   0.573   0.705   0.683   0.712   0.529   0.561   0.596
113   V         0.525   0.545   0.573   0.246   0.343   0.424   0.529   0.561   0.596
114   C         0.525   0.545   0.573   0.949   0.885   0.865   0.529   0.561   0.596
115   E         0.525   0.545   0.573   0.444   0.499   0.548   0.529   0.561   0.596
116   N         0.525   0.545   0.573   0.601   0.593   0.647   0.529   0.561   0.596
117   I         0.525   0.545   0.573   0.632   0.649   0.667   0.529   0.561   0.596
118   P         0.525   0.545   0.573   0.601   0.625   0.647   0.529   0.561   0.596
119   I         0.525   0.545   0.573   0.632   0.649   0.667   0.529   0.561   0.596
120   V         0.525   0.545   0.573   0.246   0.343   0.424   0.529   0.561   0.596
121   L         0.525   0.545   0.573   0.283   0.381   0.448   0.529   0.561   0.596
122   C         0.525   0.545   0.573   0.949   0.885   0.865   0.529   0.561   0.596
123   G         0.525   0.545   0.573   0.412   0.461   0.528   0.529   0.561   0.596
124   N         0.525   0.545   0.573   0.601   0.593   0.647   0.529   0.561   0.596
125   K         0.525   0.545   0.573   0.267   0.369   0.438   0.529   0.561   0.596
126   V         0.525   0.545   0.573   0.246   0.343   0.424   0.529   0.561   0.596
127   D         0.525   0.545   0.573   0.465   0.505   0.562   0.529   0.561   0.596
128   V         0.525   0.545   0.573   0.246   0.343   0.424   0.529   0.561   0.596
129   K         0.525   0.545   0.573   0.267   0.369   0.438   0.529   0.561   0.596
130   E         0.525   0.545   0.573   0.444   0.499   0.548   0.529   0.561   0.596
131   R         0.525   0.545   0.573   0.705   0.683   0.712   0.529   0.561   0.596
132   K         0.525   0.545   0.573   0.267   0.369   0.438   0.529   0.561   0.596
133   V         0.525   0.545   0.573   0.246   0.343   0.424   0.529   0.561   0.596
134   K         0.525   0.545   0.573   0.267   0.369   0.438   0.529   0.561   0.596
135   A         0.525   0.545   0.573   0.401   0.439   0.522   0.529   0.561   0.596
136   K         0.525   0.545   0.573   0.267   0.369   0.438   0.529   0.561   0.596
137   T        -0.698  -0.676  -1.147  -0.577  -0.565  -1.297  -0.677  -0.669  -1.353
138   I         0.525   0.545   0.573   0.632   0.649   0.667   0.529   0.561   0.596
139   T         0.525   0.545   0.573   0.496   0.532   0.581   0.529   0.561   0.596
140   F         0.525   0.545   0.573   0.549   0.578   0.614   0.529   0.561   0.596
141   H         0.525   0.545   0.573   0.889   0.845   0.827   0.529   0.561   0.596
142   R         0.525   0.545   0.573   0.705   0.683   0.712   0.529   0.561   0.596
143   K         0.525   0.545   0.573   0.267   0.369   0.438   0.529   0.561   0.596
144   K         0.525   0.545   0.573   0.267   0.369   0.438   0.529   0.561   0.596
145   N         0.525   0.545   0.573   0.601   0.593   0.647   0.529   0.561   0.596
146   L         0.525   0.545   0.573   0.283   0.381   0.448   0.529   0.561   0.596
147   Q         0.525   0.545   0.573   0.648   0.687   0.676   0.529   0.561   0.596
148   Y         0.525   0.545   0.573   0.720   0.721   0.722   0.529   0.561   0.596
149   Y         0.525   0.545   0.573   0.720   0.721   0.722   0.529   0.561   0.596
150   D         0.525   0.545   0.573   0.465   0.505   0.562   0.529   0.561   0.596
151   I         0.525   0.545   0.573   0.632   0.649   0.667   0.529   0.561   0.596
152   S         0.525   0.545   0.573   0.884   0.842   0.824   0.529   0.561   0.596
153   A         0.525   0.545   0.573   0.401   0.439   0.522   0.529   0.561   0.596
154   K         0.525   0.545   0.573   0.267   0.369   0.438   0.529   0.561   0.596
155   S         0.525   0.545   0.573   0.884   0.842   0.824   0.529   0.561   0.596
156   N         0.525   0.545   0.573   0.601   0.593   0.647   0.529   0.561   0.596
157   Y         0.525   0.545   0.573   0.720   0.721   0.722   0.529   0.561   0.596
158   N         0.525   0.545   0.573   0.601   0.593   0.647   0.529   0.561   0.596
159   F         0.525   0.545   0.573   0.549   0.578   0.614   0.529   0.561   0.596
160   E         0.525   0.545   0.573   0.444   0.499   0.548   0.529   0.561   0.596
161   K         0.525   0.545   0.573   0.267   0.369   0.438   0.529   0.561   0.596
162   P         0.525   0.545   0.573   0.601   0.625   0.647   0.529   0.561   0.596
163   F         0.525   0.545   0.573   0.549   0.578   0.614   0.529   0.561   0.596
164   L         0.525   0.545   0.573   0.283   0.381   0.448   0.529   0.561   0.596
165   W         0.525   0.545   0.573   0.954   0.894   0.868   0.529   0.561   0.596
166   L         0.525   0.545   0.573   0.283   0.381   0.448   0.529   0.561   0.596
167   A         0.525   0.545   0.573   0.401   0.439   0.522   0.529   0.561   0.596
168   R         0.525   0.545   0.573   0.705   0.683   0.712   0.529   0.561   0.596
169   K         0.525   0.545   0.573   0.267   0.369   0.438   0.529   0.561   0.596
170   L         0.525   0.545   0.573   0.283   0.381   0.448   0.529   0.561   0.596
171   A        -1.884  -1.473  -1.173  -2.712  -2.037  -1.468  -2.486  -1.878  -1.394
172   G         0.525   0.545   0.573   0.412   0.461   0.528   0.529   0.561   0.596
173   N         0.525   0.545   0.573   0.601   0.593   0.647   0.529   0.561   0.596
174   P        -0.698  -0.865  -1.151  -0.447  -0.700  -1.173  -0.677  -0.933  -1.360
175   Q        -2.991  -3.258  -2.918  -2.440  -2.968  -2.522  -2.637  -2.890  -2.388
176   L         0.525   0.545   0.573   0.283   0.381   0.448   0.529   0.561   0.596
177   E        -1.333  -0.612  -1.139  -1.518  -0.507  -1.285  -1.581  -0.585  -1.340
178   F         0.525   0.545   0.573   0.549   0.578   0.614   0.529   0.561   0.596
179   V         0.525   0.545   0.573   0.246   0.343   0.424   0.529   0.561   0.596
180   A         0.525   0.545   0.573   0.401   0.439   0.522   0.529   0.561   0.596
181   S        -1.873  -2.309  -2.188  -1.245  -2.082  -2.035  -1.732  -2.283  -2.051
182   P         0.525   0.545   0.573   0.601   0.625   0.647   0.529   0.561   0.596
183   A         0.525   0.545   0.573   0.401   0.439   0.522   0.529   0.561   0.596
184   L         0.525   0.545   0.573   0.283   0.381   0.448   0.529   0.561   0.596
185   A        -0.698  -1.071  -1.157  -0.634  -1.157  -1.241  -0.677  -1.236  -1.368
186   P         0.525   0.545   0.573   0.601   0.625   0.647   0.529   0.561   0.596
187   P        -0.698  -1.071  -1.157  -0.472  -1.053  -1.262  -0.677  -1.236  -1.368
188   E        -0.698  -0.865  -1.151  -0.618  -0.839  -1.295  -0.677  -0.933  -1.360
189   V        -1.873  -2.309  -2.188  -1.883  -2.544  -2.309  -1.732  -2.283  -2.051
190   Q        -1.873  -2.309  -2.188  -1.514  -2.288  -2.227  -1.732  -2.283  -2.051
191   V        -0.698  -1.071  -1.157  -0.799  -1.262  -1.348  -0.677  -1.236  -1.368
192   D         0.525   0.545   0.573   0.465   0.505   0.562   0.529   0.561   0.596
193   E        -3.939  -3.379  -2.914  -4.137  -3.322  -2.662  -3.692  -3.011  -2.385
194   Q        -2.964  -2.392  -2.187  -3.137  -2.469  -2.137  -3.089  -2.439  -2.050
195   L        -0.698  -1.071  -1.157  -0.810  -1.322  -1.490  -0.677  -1.236  -1.368
196   M        -0.698  -0.676  -1.147  -0.188  -0.267  -1.131  -0.677  -0.669  -1.353
197   Q        -1.873  -1.988  -2.182  -1.486  -1.724  -2.141  -1.732  -1.891  -2.044
198   Q        -3.505  -3.101  -2.914  -3.374  -3.068  -2.695  -3.240  -2.806  -2.385
199   Y        -0.698  -1.071  -1.157  -0.291  -0.838  -1.006  -0.677  -1.236  -1.368
200   Q        -0.698  -0.865  -1.151  -0.387  -0.619  -1.109  -0.677  -0.933  -1.360
201   Q        -1.873  -2.309  -2.188  -1.511  -2.286  -2.219  -1.732  -2.283  -2.051
202   E        -0.698  -1.071  -1.157  -0.622  -1.156  -1.317  -0.677  -1.236  -1.368
203   M        -0.698  -1.071  -1.157  -0.188  -0.861  -1.156  -0.677  -1.236  -1.368
204   E        -1.873  -1.988  -2.182  -1.659  -1.873  -2.152  -1.732  -1.891  -2.044
205   Q        -1.707  -1.439  -1.160  -2.002  -1.695  -1.247  -2.184  -1.820  -1.373
206   A         0.525   0.545   0.573   0.401   0.439   0.522   0.529   0.561   0.596
207   T        -2.451  -2.587  -2.190  -2.485  -2.836  -2.170  -2.486  -2.618  -2.053
208   A        -0.698  -0.865  -1.151  -0.641  -0.861  -1.256  -0.677  -0.933  -1.360
209   L        -1.707  -1.439  -1.160  -2.122  -1.592  -1.165  -2.184  -1.820  -1.373
210   P         0.525   0.545   0.573   0.601   0.625   0.647   0.529   0.561   0.596
211   L         0.525   0.545   0.573   0.283   0.381   0.448   0.529   0.561   0.596
212   P         0.525   0.545   0.573   0.601   0.625   0.647   0.529   0.561   0.596
213   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
214   D        -0.698  -0.865  -1.151  -0.603  -0.840  -1.305  -0.677  -0.933  -1.360
215   E         0.525   0.545   0.573   0.444   0.499   0.548   0.529   0.561   0.596
216   D         0.525   0.545   0.573   0.465   0.505   0.562   0.529   0.561   0.596
217   D         0.525   0.545   0.573   0.465   0.505   0.562   0.529   0.561   0.596
218   A        -0.698  -0.865  -1.151  -0.664  -0.904  -1.330  -0.677  -0.933  -1.360
219   D         0.525   0.545   0.573   0.465   0.505   0.562   0.529   0.561   0.596
220   L        -0.698  -1.071  -1.157  -0.771  -1.247  -1.364  -0.677  -1.236  -1.368
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P32835
Output conservation file - output/P32835
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
