1     M         1.818   1.839   1.893   2.124   2.163   2.251   1.992   2.019   2.101
2     S         1.818   1.839   1.893   1.813   1.835   1.937   1.992   2.019   2.101
3     N         1.818   1.839   1.893   1.991   2.033   2.117   1.992   2.019   2.101
4     Q         0.537   0.451   0.437   0.753   0.630   0.608   0.439   0.290   0.255
5     A        -0.550  -0.590  -0.574  -0.429  -0.537  -0.491  -0.597  -0.644  -0.611
6     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
7     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
8     L         1.818   1.839   1.893   1.455   1.497   1.575   1.992   2.019   2.101
9     Y         0.537   0.600   0.478   0.300   0.390   0.251   0.439   0.540   0.326
10    E        -0.550  -0.590  -0.574  -0.686  -0.739  -0.701  -0.597  -0.644  -0.611
11    K        -0.550  -0.562  -0.565  -0.384  -0.397  -0.410  -0.597  -0.610  -0.600
12    L         0.537   0.451   0.437   0.165   0.036   0.012   0.439   0.290   0.255
13    E        -0.550  -0.466  -0.536  -0.540  -0.396  -0.527  -0.597  -0.480  -0.562
14    Q        -0.550  -0.590  -0.574  -0.350  -0.412  -0.376  -0.597  -0.644  -0.611
15    T         0.537   0.451   0.437   0.574   0.448   0.428   0.439   0.290   0.255
16    R         0.537   0.600   0.478   0.597   0.696   0.521   0.439   0.540   0.326
17    T         0.537   0.600   0.478   0.337   0.443   0.232   0.439   0.540   0.326
18    I        -1.339  -1.318  -1.288  -1.512  -1.431  -1.428  -1.115  -1.082  -1.027
19    L         1.818   1.839   1.893   1.455   1.497   1.575   1.992   2.019   2.101
20    S         0.537   0.451   0.437   0.530   0.382   0.370   0.439   0.290   0.255
21    V        -0.550  -0.590  -0.574  -0.528  -0.623  -0.578  -0.597  -0.644  -0.611
22    K         0.537   0.566   0.470   0.688   0.731   0.562   0.439   0.483   0.312
23    L         0.537   0.451   0.437   0.285   0.185   0.183   0.439   0.290   0.255
24    A        -0.550  -0.590  -0.574  -0.389  -0.479  -0.443  -0.597  -0.644  -0.611
25    E        -0.550  -0.590  -0.574  -0.534  -0.590  -0.524  -0.597  -0.644  -0.611
26    L         0.537   0.451   0.437   0.194   0.077   0.054   0.439   0.290   0.255
27    I        -0.550  -0.570  -0.569  -0.339  -0.392  -0.375  -0.597  -0.620  -0.605
28    N         0.240   0.258   0.305   0.210   0.237   0.318  -0.079  -0.050   0.020
29    M        -0.550  -0.570  -0.569  -0.904  -0.947  -0.904  -0.597  -0.620  -0.605
30    T        -0.550  -0.499  -0.541  -0.481  -0.399  -0.483  -0.597  -0.524  -0.569
31    T        -1.339  -1.318  -1.288  -1.303  -1.264  -1.216  -1.115  -1.082  -1.027
32    I        -0.550  -0.562  -0.565  -0.344  -0.376  -0.364  -0.597  -0.610  -0.600
33    A        -0.550  -0.570  -0.569  -0.729  -0.787  -0.746  -0.597  -0.620  -0.605
34    D        -0.684  -0.673  -0.628  -0.533  -0.510  -0.437  -0.770  -0.748  -0.679
35    R        -0.684  -0.673  -0.628  -0.401  -0.381  -0.304  -0.770  -0.748  -0.679
36    N        -0.684  -0.673  -0.628  -0.704  -0.681  -0.610  -0.770  -0.748  -0.679
37    D         0.369   0.371   0.357   0.526   0.531   0.504   0.151   0.153   0.115
38    D        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
39    D        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
40    E        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
41    G        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
42    S        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
43    F        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
44    A        -0.684  -0.668  -0.628  -0.581  -0.583  -0.485  -0.770  -0.741  -0.679
45    Q        -1.339  -1.318  -1.288  -1.124  -1.081  -1.035  -1.115  -1.082  -1.027
46    E        -0.550  -0.590  -0.574  -0.767  -0.832  -0.798  -0.597  -0.644  -0.611
47    N        -1.339  -1.318  -1.288  -1.212  -1.189  -1.124  -1.115  -1.082  -1.027
48    S         0.537   0.614   0.484   0.640   0.734   0.591   0.439   0.562   0.337
49    E        -1.339  -1.318  -1.288  -1.146  -1.098  -1.057  -1.115  -1.082  -1.027
50    L         1.818   1.839   1.893   1.455   1.497   1.575   1.992   2.019   2.101
51    A         0.537   0.614   0.484   0.736   0.850   0.634   0.439   0.562   0.337
52    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
53    V         0.537   0.451   0.437   0.706   0.571   0.559   0.439   0.290   0.255
54    A        -1.339  -1.318  -1.288  -1.389  -1.404  -1.304  -1.115  -1.082  -1.027
55    T         0.537   0.566   0.470   0.485   0.535   0.360   0.439   0.483   0.312
56    T        -0.684  -0.673  -0.628  -0.861  -0.847  -0.769  -0.770  -0.748  -0.679
57    S         0.369   0.390   0.362   0.581   0.616   0.539   0.151   0.185   0.123
58    V         0.369   0.390   0.366   0.156   0.194   0.089   0.151   0.185   0.130
59    M        -0.550  -0.590  -0.574  -0.169  -0.212  -0.192  -0.597  -0.644  -0.611
60    M         0.537   0.451   0.437   0.667   0.489   0.425   0.439   0.290   0.255
61    V         0.537   0.566   0.470   0.605   0.643   0.479   0.439   0.483   0.312
62    N         0.537   0.451   0.437   0.601   0.443   0.391   0.439   0.290   0.255
63    N         0.537   0.451   0.437   0.601   0.443   0.391   0.439   0.290   0.255
64    Q        -0.550  -0.590  -0.574  -0.350  -0.435  -0.376  -0.597  -0.644  -0.611
65    T         1.818   1.839   1.893   1.827   1.871   1.951   1.992   2.019   2.101
66    M        -0.550  -0.562  -0.565  -0.600  -0.630  -0.682  -0.597  -0.610  -0.600
67    Q         0.537   0.451   0.437   0.731   0.602   0.578   0.439   0.290   0.255
68    L         0.537   0.451   0.437   0.228   0.117   0.102   0.439   0.290   0.255
69    I         0.240   0.256   0.304   0.181   0.205   0.287  -0.079  -0.052   0.019
70    K         0.240   0.256   0.304   0.324   0.347   0.438  -0.079  -0.052   0.019
71    N        -0.550  -0.570  -0.569  -0.311  -0.380  -0.366  -0.597  -0.620  -0.605
72    V        -0.550  -0.590  -0.574  -0.661  -0.779  -0.737  -0.597  -0.644  -0.611
73    Q         0.537   0.451   0.437   0.601   0.446   0.391   0.439   0.290   0.255
74    D         1.818   1.839   1.893   2.004   2.031   2.131   1.992   2.019   2.101
75    L         1.818   1.839   1.893   1.455   1.497   1.575   1.992   2.019   2.101
76    L         0.537   0.614   0.484   0.194   0.315   0.124   0.439   0.562   0.337
77    I        -1.339  -1.318  -1.288  -1.265  -1.175  -1.177  -1.115  -1.082  -1.027
78    L        -0.550  -0.590  -0.574  -0.884  -0.927  -0.885  -0.597  -0.644  -0.611
79    T         1.818   1.839   1.893   1.827   1.871   1.951   1.992   2.019   2.101
80    R         1.818   1.839   1.893   2.150   2.182   2.278   1.992   2.019   2.101
81    S         0.537   0.614   0.484   0.530   0.636   0.449   0.439   0.562   0.337
82    I         0.537   0.451   0.437   0.574   0.402   0.334   0.439   0.290   0.255
83    K         0.240   0.258   0.305   0.324   0.341   0.431  -0.079  -0.050   0.020
84    E         1.818   1.839   1.893   1.673   1.710   1.795   1.992   2.019   2.101
85    K         0.537   0.451   0.437   0.770   0.624   0.615   0.439   0.290   0.255
86    W         1.818   1.839   1.893   2.305   2.342   2.435   1.992   2.019   2.101
87    L        -0.550  -0.590  -0.574  -0.884  -0.917  -0.885  -0.597  -0.644  -0.611
88    L         1.818   1.839   1.893   1.455   1.497   1.575   1.992   2.019   2.101
89    N        -1.339  -1.318  -1.288  -1.212  -1.152  -1.124  -1.115  -1.082  -1.027
90    Q         1.818   1.839   1.893   1.991   2.036   2.117   1.992   2.019   2.101
91    I         0.537   0.566   0.470   0.574   0.632   0.409   0.439   0.483   0.312
92    P         0.537   0.451   0.437   1.014   0.875   0.858   0.439   0.290   0.255
93    V        -0.550  -0.570  -0.569  -0.582  -0.585  -0.575  -0.597  -0.620  -0.605
94    T        -1.339  -1.318  -1.288  -1.257  -1.214  -1.170  -1.115  -1.082  -1.027
95    E        -0.684  -0.666  -0.628  -1.228  -1.174  -1.140  -0.770  -0.739  -0.679
96    H        -0.684  -0.666  -0.628  -0.566  -0.537  -0.470  -0.770  -0.739  -0.679
97    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
98    S        -0.550  -0.499  -0.541  -0.594  -0.511  -0.602  -0.597  -0.524  -0.569
99    K        -0.550  -0.570  -0.569  -0.546  -0.553  -0.533  -0.597  -0.620  -0.605
100   V        -1.339  -1.318  -1.288  -1.659  -1.646  -1.577  -1.115  -1.082  -1.027
101   T        -0.550  -0.499  -0.541  -0.552  -0.472  -0.552  -0.597  -0.524  -0.569
102   R        -1.339  -1.318  -1.288  -0.970  -0.928  -0.879  -1.115  -1.082  -1.027
103   F        -0.550  -0.562  -0.565  -0.074  -0.094  -0.097  -0.597  -0.610  -0.600
104   D        -0.550  -0.466  -0.536  -0.686  -0.564  -0.651  -0.597  -0.480  -0.562
105   E        -1.339  -1.318  -1.288  -1.110  -1.068  -1.021  -1.115  -1.082  -1.027
106   K        -0.550  -0.570  -0.569  -0.673  -0.699  -0.681  -0.597  -0.620  -0.605
107   Q        -0.550  -0.570  -0.569  -0.938  -1.009  -0.992  -0.597  -0.620  -0.605
108   I        -1.339  -1.318  -1.288  -1.454  -1.377  -1.369  -1.115  -1.082  -1.027
109   E        -1.339  -1.318  -1.288  -1.732  -1.661  -1.651  -1.115  -1.082  -1.027
110   E         1.818   1.839   1.893   1.673   1.710   1.795   1.992   2.019   2.101
111   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
112   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
113   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
114   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
115   L         1.818   1.839   1.893   1.455   1.497   1.575   1.992   2.019   2.101
116   L         0.537   0.566   0.470   0.165   0.214   0.064   0.439   0.483   0.312
117   D        -0.550  -0.490  -0.543  -0.540  -0.432  -0.543  -0.597  -0.512  -0.572
118   N        -1.339  -1.318  -1.288  -1.397  -1.355  -1.312  -1.115  -1.082  -1.027
119   C        -0.550  -0.590  -0.574  -0.196  -0.278  -0.266  -0.597  -0.644  -0.611
120   I        -1.339  -1.318  -1.288  -1.242  -1.239  -1.155  -1.115  -1.082  -1.027
121   E        -0.550  -0.562  -0.565  -0.767  -0.787  -0.785  -0.597  -0.610  -0.600
122   T         0.537   0.614   0.484   0.439   0.577   0.328   0.439   0.562   0.337
123   F        -1.339  -1.318  -1.288  -1.342  -1.340  -1.256  -1.115  -1.082  -1.027
124   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
125   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
126   V        -0.550  -0.490  -0.543  -0.661  -0.558  -0.671  -0.597  -0.512  -0.572
127   A        -1.339  -1.318  -1.288  -1.182  -1.158  -1.094  -1.115  -1.082  -1.027
128   E        -0.550  -0.570  -0.569  -0.522  -0.562  -0.574  -0.597  -0.620  -0.605
129   K        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
130   T         0.369   0.399   0.387   0.336   0.392   0.370   0.151   0.201   0.167
131   T        -1.339  -1.318  -1.288  -1.053  -1.013  -0.963  -1.115  -1.082  -1.027
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P32570
Output conservation file - output/P32570
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
