1     M         0.985   1.005   1.073   1.377   1.368   1.429   1.026   1.056   1.154
2     S         0.985   1.005   1.073   0.999   1.021   1.128   1.026   1.056   1.154
3     A         0.065   0.289  -0.152   0.150   0.424  -0.159   0.037   0.341  -0.334
4     P        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
5     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
6     Q        -1.436  -1.289  -1.036  -1.833  -1.646  -1.226  -1.611  -1.417  -1.071
7     A        -1.979  -1.799  -1.582  -2.056  -1.725  -1.462  -1.877  -1.648  -1.345
8     K         0.985   1.005   1.073   0.777   0.837   0.951   1.026   1.056   1.154
9     I         0.985   1.005   1.073   1.067   1.095   1.182   1.026   1.056   1.154
10    L        -1.424  -1.680  -1.578  -1.532  -1.907  -1.701  -1.246  -1.516  -1.341
11    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
12    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
13    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
14    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
15    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
16    S        -0.666  -0.870  -0.981  -0.545  -0.870  -1.109  -0.615  -0.881  -1.003
17    Q        -1.035  -0.943  -0.984  -1.024  -0.837  -0.973  -1.119  -0.973  -1.008
18    A        -2.368  -2.238  -2.009  -2.200  -1.973  -1.681  -2.003  -1.840  -1.512
19    P         0.985   1.005   1.073   1.088   1.112   1.199   1.026   1.056   1.154
20    T        -0.499  -0.444  -0.262  -0.660  -0.616  -0.322  -0.867  -0.797  -0.521
21    E         0.985   1.005   1.073   0.974   1.008   1.108   1.026   1.056   1.154
22    L         0.140   0.007  -0.176  -0.024  -0.161  -0.392   0.143  -0.058  -0.373
23    E         0.140   0.007  -0.176   0.241   0.078  -0.201   0.143  -0.058  -0.373
24    L         0.140  -0.036  -0.174  -0.024  -0.220  -0.389   0.143  -0.122  -0.370
25    Q        -1.424  -1.519  -1.561  -1.271  -1.459  -1.651  -1.246  -1.361  -1.325
26    V         0.140   0.358  -0.146   0.133   0.398  -0.231   0.143   0.430  -0.324
27    A         0.985   1.005   1.073   0.982   1.006   1.115   1.026   1.056   1.154
28    Q         0.140  -0.036  -0.174   0.329   0.121  -0.121   0.143  -0.122  -0.370
29    A         0.140   0.007  -0.176   0.290   0.132  -0.086   0.143  -0.058  -0.373
30    F        -0.277  -0.391  -0.258  -0.322  -0.615  -0.461  -0.488  -0.704  -0.515
31    V        -1.348  -1.194  -1.028  -1.513  -1.241  -0.967  -1.498  -1.316  -1.062
32    E         0.140   0.401  -0.145   0.375   0.675  -0.092   0.143   0.485  -0.321
33    L         0.985   1.005   1.073   0.710   0.770   0.898   1.026   1.056   1.154
34    E         0.985   1.005   1.073   0.974   1.008   1.108   1.026   1.056   1.154
35    N        -1.201  -0.980  -1.003  -1.104  -0.883  -0.823  -1.372  -1.122  -1.034
36    S        -1.348  -1.147  -1.016  -1.403  -1.093  -0.886  -1.498  -1.244  -1.048
37    S        -0.666  -0.671  -0.960  -0.477  -0.511  -0.916  -0.615  -0.649  -0.978
38    P        -0.962  -1.091  -1.016  -0.895  -1.144  -1.104  -0.993  -1.163  -1.047
39    E        -0.499  -0.225  -0.197  -0.669  -0.184  -0.192  -0.867  -0.423  -0.410
40    L        -0.666  -0.834  -0.983  -0.694  -0.862  -0.974  -0.615  -0.832  -1.007
41    K         0.140   0.007  -0.176   0.022  -0.124  -0.403   0.143  -0.058  -0.373
42    A        -0.666  -0.834  -0.983  -0.549  -0.810  -1.095  -0.615  -0.832  -1.007
43    E        -0.666  -0.653  -0.959  -0.502  -0.518  -0.931  -0.615  -0.629  -0.977
44    L         0.985   1.005   1.073   0.710   0.770   0.898   1.026   1.056   1.154
45    R         0.985   1.005   1.073   0.922   0.938   1.066   1.026   1.056   1.154
46    P        -0.666  -0.535  -0.952  -0.456  -0.306  -0.992  -0.615  -0.466  -0.968
47    L         0.140   0.092  -0.167  -0.022  -0.044  -0.375   0.143   0.069  -0.359
48    Q         0.985   1.005   1.073   1.055   1.103   1.172   1.026   1.056   1.154
49    F         0.140   0.007  -0.176   0.392   0.207  -0.111   0.143  -0.058  -0.373
50    K        -1.424  -1.491  -1.559  -1.463  -1.579  -1.640  -1.246  -1.336  -1.323
51    S         0.985   1.005   1.073   0.999   1.021   1.128   1.026   1.056   1.154
52    I        -1.201  -0.939  -0.996  -1.368  -1.015  -1.047  -1.372  -1.067  -1.025
53    R        -0.277  -0.039  -0.185  -0.511  -0.129  -0.398  -0.488  -0.126  -0.389
54    E         0.985   1.005   1.073   0.974   1.008   1.108   1.026   1.056   1.154
55    I        -0.666  -0.834  -0.983  -0.496  -0.762  -1.117  -0.615  -0.832  -1.007
56    D        -0.570  -0.385  -0.230  -0.839  -0.535  -0.290  -0.993  -0.693  -0.465
57    V         0.985   1.005   1.073   0.865   0.901   1.021   1.026   1.056   1.154
58    A        -1.979  -1.799  -1.582  -1.896  -1.628  -1.301  -1.877  -1.648  -1.345
59    G        -1.035  -1.083  -1.005  -0.846  -0.914  -0.829  -1.119  -1.157  -1.035
60    G         0.985   1.005   1.073   1.181   1.182   1.272   1.026   1.056   1.154
61    K         0.985   1.005   1.073   0.777   0.837   0.951   1.026   1.056   1.154
62    K         0.985   1.005   1.073   0.777   0.837   0.951   1.026   1.056   1.154
63    A         0.985   1.005   1.073   0.982   1.006   1.115   1.026   1.056   1.154
64    L        -0.277  -0.391  -0.258  -0.622  -0.782  -0.529  -0.488  -0.704  -0.515
65    A        -0.499  -0.451  -0.248  -0.858  -0.824  -0.465  -0.867  -0.809  -0.497
66    I        -0.499  -0.437  -0.262  -0.854  -0.743  -0.434  -0.867  -0.785  -0.521
67    F         0.985   1.005   1.073   1.135   1.151   1.236   1.026   1.056   1.154
68    V         0.985   1.005   1.073   0.865   0.901   1.021   1.026   1.056   1.154
69    P         0.985   1.005   1.073   1.088   1.112   1.199   1.026   1.056   1.154
70    V        -1.424  -1.230  -1.534  -1.243  -1.021  -1.315  -1.246  -1.045  -1.299
71    P         0.985   1.005   1.073   1.088   1.112   1.199   1.026   1.056   1.154
72    S        -0.666  -0.834  -0.983  -0.546  -0.816  -1.116  -0.615  -0.832  -1.007
73    L         0.985   1.005   1.073   0.710   0.770   0.898   1.026   1.056   1.154
74    A        -2.368  -2.228  -2.006  -2.330  -2.155  -1.794  -2.003  -1.834  -1.511
75    G        -0.499  -0.384  -0.252  -0.690  -0.515  -0.293  -0.867  -0.692  -0.503
76    F        -1.201  -1.133  -1.002  -1.093  -1.027  -0.859  -1.372  -1.254  -1.032
77    H        -0.277  -0.039  -0.185  -0.097   0.253  -0.067  -0.488  -0.126  -0.389
78    K        -0.277  -0.267  -0.245  -0.606  -0.581  -0.550  -0.488  -0.493  -0.492
79    V        -0.666  -0.834  -0.983  -0.547  -0.742  -0.894  -0.615  -0.832  -1.007
80    Q         0.985   1.005   1.073   1.055   1.103   1.172   1.026   1.056   1.154
81    T        -1.737  -1.669  -1.570  -1.585  -1.558  -1.386  -1.624  -1.553  -1.335
82    K        -0.499  -0.225  -0.197  -0.960  -0.445  -0.422  -0.867  -0.423  -0.410
83    L         0.985   1.005   1.073   0.710   0.770   0.898   1.026   1.056   1.154
84    T         0.985   1.005   1.073   1.126   1.132   1.229   1.026   1.056   1.154
85    R         0.985   1.005   1.073   0.922   0.938   1.066   1.026   1.056   1.154
86    E         0.985   1.005   1.073   0.974   1.008   1.108   1.026   1.056   1.154
87    L         0.985   1.005   1.073   0.710   0.770   0.898   1.026   1.056   1.154
88    E         0.985   1.005   1.073   0.974   1.008   1.108   1.026   1.056   1.154
89    K         0.985   1.005   1.073   0.777   0.837   0.951   1.026   1.056   1.154
90    K         0.985   1.005   1.073   0.777   0.837   0.951   1.026   1.056   1.154
91    F         0.985   1.005   1.073   1.135   1.151   1.236   1.026   1.056   1.154
92    Q        -1.979  -1.826  -1.577  -2.036  -1.817  -1.446  -1.877  -1.673  -1.340
93    D         0.985   1.005   1.073   1.131   1.145   1.233   1.026   1.056   1.154
94    R         0.985   1.005   1.073   0.922   0.938   1.066   1.026   1.056   1.154
95    H         0.985   1.005   1.073   1.300   1.300   1.367   1.026   1.056   1.154
96    V         0.985   1.005   1.073   0.865   0.901   1.021   1.026   1.056   1.154
97    I        -1.201  -1.198  -1.016  -1.472  -1.457  -1.196  -1.372  -1.316  -1.049
98    F         0.140  -0.036  -0.174   0.392   0.147  -0.108   0.143  -0.122  -0.370
99    L        -0.666  -0.941  -0.996  -0.798  -1.169  -1.231  -0.615  -0.970  -1.022
100   A         0.985   1.005   1.073   0.982   1.006   1.115   1.026   1.056   1.154
101   E        -0.666  -0.941  -0.996  -0.512  -0.898  -1.012  -0.615  -0.970  -1.022
102   R         0.985   1.005   1.073   0.922   0.938   1.066   1.026   1.056   1.154
103   R         0.985   1.005   1.073   0.922   0.938   1.066   1.026   1.056   1.154
104   I         0.985   1.005   1.073   1.067   1.095   1.182   1.026   1.056   1.154
105   L         0.985   1.005   1.073   0.710   0.770   0.898   1.026   1.056   1.154
106   P         0.985   1.005   1.073   1.088   1.112   1.199   1.026   1.056   1.154
107   K         0.140  -0.036  -0.174   0.013  -0.202  -0.424   0.143  -0.122  -0.370
108   P         0.140   0.092  -0.167   0.334   0.269  -0.161   0.143   0.069  -0.359
109   S        -1.737  -1.699  -1.574  -1.707  -1.706  -1.490  -1.624  -1.578  -1.338
110   R         0.985   1.005   1.073   0.922   0.938   1.066   1.026   1.056   1.154
111   T        -1.424  -1.197  -1.524  -1.435  -1.155  -1.541  -1.246  -1.024  -1.289
112   S        -0.277  -0.193  -0.232  -0.369  -0.251  -0.347  -0.488  -0.371  -0.469
113   R         0.140   0.092  -0.167   0.129   0.053  -0.387   0.143   0.069  -0.359
114   Q        -2.126  -2.190  -2.015  -1.936  -2.091  -1.902  -1.750  -1.791  -1.517
115   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
116   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
117   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
118   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
119   V        -2.368  -2.162  -2.003  -2.306  -2.048  -1.847  -2.003  -1.776  -1.508
120   Q         0.985   1.005   1.073   1.055   1.103   1.172   1.026   1.056   1.154
121   K         0.140   0.092  -0.167   0.038   0.015  -0.346   0.143   0.069  -0.359
122   R         0.985   1.005   1.073   0.922   0.938   1.066   1.026   1.056   1.154
123   P         0.985   1.005   1.073   1.088   1.112   1.199   1.026   1.056   1.154
124   R         0.140   0.092  -0.167   0.226   0.166  -0.128   0.143   0.069  -0.359
125   S         0.985   1.005   1.073   0.999   1.021   1.128   1.026   1.056   1.154
126   R         0.985   1.005   1.073   0.922   0.938   1.066   1.026   1.056   1.154
127   T         0.985   1.005   1.073   1.126   1.132   1.229   1.026   1.056   1.154
128   L         0.985   1.005   1.073   0.710   0.770   0.898   1.026   1.056   1.154
129   T         0.140   0.358  -0.146   0.374   0.616  -0.089   0.143   0.430  -0.324
130   A         0.140   0.092  -0.167   0.199   0.133  -0.316   0.143   0.069  -0.359
131   V         0.985   1.005   1.073   0.865   0.901   1.021   1.026   1.056   1.154
132   H         0.985   1.005   1.073   1.300   1.300   1.367   1.026   1.056   1.154
133   D        -0.666  -0.834  -0.983  -0.320  -0.556  -0.807  -0.615  -0.832  -1.007
134   K        -0.499  -0.451  -0.248  -0.979  -0.857  -0.494  -0.867  -0.809  -0.497
135   I         0.140   0.092  -0.167   0.275   0.210  -0.278   0.143   0.069  -0.359
136   L         0.985   1.005   1.073   0.710   0.770   0.898   1.026   1.056   1.154
137   E         0.140  -0.036  -0.174   0.240   0.016  -0.200   0.143  -0.122  -0.370
138   D         0.985   1.005   1.073   1.131   1.145   1.233   1.026   1.056   1.154
139   L         0.140   0.007  -0.176  -0.033  -0.170  -0.416   0.143  -0.058  -0.373
140   V         0.985   1.005   1.073   0.865   0.901   1.021   1.026   1.056   1.154
141   F         0.140  -0.036  -0.174   0.424   0.193  -0.022   0.143  -0.122  -0.370
142   P         0.985   1.005   1.073   1.088   1.112   1.199   1.026   1.056   1.154
143   T         0.140  -0.036  -0.174   0.356   0.083  -0.191   0.143  -0.122  -0.370
144   E         0.985   1.005   1.073   0.974   1.008   1.108   1.026   1.056   1.154
145   I         0.985   1.005   1.073   1.067   1.095   1.182   1.026   1.056   1.154
146   V        -0.499  -0.439  -0.262  -0.854  -0.746  -0.434  -0.867  -0.788  -0.521
147   G         0.985   1.005   1.073   1.181   1.182   1.272   1.026   1.056   1.154
148   K         0.985   1.005   1.073   0.777   0.837   0.951   1.026   1.056   1.154
149   R         0.985   1.005   1.073   0.922   0.938   1.066   1.026   1.056   1.154
150   V        -0.666  -0.941  -0.996  -0.656  -1.069  -1.168  -0.615  -0.970  -1.022
151   R         0.140   0.092  -0.167   0.138   0.065  -0.361   0.143   0.069  -0.359
152   Y        -0.666  -0.941  -0.996  -0.218  -0.665  -0.864  -0.615  -0.970  -1.022
153   L        -1.424  -1.680  -1.578  -1.553  -1.933  -1.746  -1.246  -1.516  -1.341
154   V        -0.666  -0.941  -0.996  -0.612  -0.997  -1.061  -0.615  -0.970  -1.022
155   G        -0.277  -0.391  -0.258  -0.147  -0.394  -0.209  -0.488  -0.704  -0.515
156   G         0.985   1.005   1.073   1.181   1.182   1.272   1.026   1.056   1.154
157   N        -1.424  -1.680  -1.578  -0.920  -1.396  -1.322  -1.246  -1.516  -1.341
158   K         0.140   0.092  -0.167   0.013  -0.018  -0.412   0.143   0.069  -0.359
159   I        -0.277  -0.391  -0.258  -0.257  -0.477  -0.279  -0.488  -0.704  -0.515
160   Q        -0.666  -0.941  -0.996  -0.476  -0.882  -1.070  -0.615  -0.970  -1.022
161   K         0.985   1.005   1.073   0.777   0.837   0.951   1.026   1.056   1.154
162   V         0.140   0.342  -0.147   0.123   0.375  -0.257   0.143   0.410  -0.326
163   L        -1.035  -1.189  -1.022  -1.276  -1.438  -1.112  -1.119  -1.304  -1.055
164   L         0.985   1.005   1.073   0.710   0.770   0.898   1.026   1.056   1.154
165   D         0.140   0.342  -0.147   0.421   0.641   0.019   0.143   0.410  -0.326
166   S        -0.666  -0.941  -0.996  -0.449  -0.834  -0.906  -0.615  -0.970  -1.022
167   K         0.140   0.007  -0.176   0.013  -0.139  -0.427   0.143  -0.058  -0.373
168   D         0.140  -0.036  -0.174   0.375   0.121  -0.147   0.143  -0.122  -0.370
169   V        -2.368  -2.205  -2.010  -2.429  -2.213  -1.885  -2.003  -1.807  -1.513
170   Q        -1.832  -1.839  -1.579  -1.683  -1.621  -1.236  -1.750  -1.684  -1.342
171   Q        -2.904  -2.719  -2.376  -2.631  -2.388  -1.859  -2.255  -2.039  -1.636
172   I        -0.570  -0.433  -0.252  -0.964  -0.736  -0.426  -0.993  -0.777  -0.504
173   D         0.985   1.005   1.073   1.131   1.145   1.233   1.026   1.056   1.154
174   Y        -0.277  -0.183  -0.233   0.011   0.117  -0.010  -0.488  -0.354  -0.472
175   K         0.140  -0.036  -0.174   0.013  -0.202  -0.424   0.143  -0.122  -0.370
176   L         0.985   1.005   1.073   0.710   0.770   0.898   1.026   1.056   1.154
177   E        -1.201  -1.187  -1.017  -1.289  -1.221  -0.931  -1.372  -1.310  -1.050
178   S         0.140  -0.036  -0.174   0.266   0.028  -0.182   0.143  -0.122  -0.370
179   F         0.140  -0.036  -0.174   0.424   0.193  -0.022   0.143  -0.122  -0.370
180   Q        -0.277  -0.391  -0.258  -0.310  -0.528  -0.356  -0.488  -0.704  -0.515
181   A        -1.348  -1.190  -1.026  -1.548  -1.325  -1.058  -1.498  -1.305  -1.060
182   V        -0.277  -0.039  -0.185  -0.589  -0.183  -0.467  -0.488  -0.126  -0.389
183   Y         0.985   1.005   1.073   1.292   1.290   1.361   1.026   1.056   1.154
184   N        -2.126  -2.045  -2.006  -1.577  -1.563  -1.557  -1.750  -1.670  -1.510
185   K         0.140  -0.036  -0.174   0.013  -0.202  -0.424   0.143  -0.122  -0.370
186   L         0.985   1.005   1.073   0.710   0.770   0.898   1.026   1.056   1.154
187   T         0.985   1.005   1.073   1.126   1.132   1.229   1.026   1.056   1.154
188   G         0.985   1.005   1.073   1.181   1.182   1.272   1.026   1.056   1.154
189   K         0.985   1.005   1.073   0.777   0.837   0.951   1.026   1.056   1.154
190   Q        -1.035  -1.130  -1.022  -0.941  -1.071  -0.956  -1.119  -1.250  -1.056
191   I        -0.499  -0.225  -0.197  -0.854  -0.388  -0.339  -0.867  -0.423  -0.410
192   V        -0.666  -0.941  -0.996  -0.656  -1.069  -1.168  -0.615  -0.970  -1.022
193   F         0.985   1.005   1.073   1.135   1.151   1.236   1.026   1.056   1.154
194   E         0.985   1.005   1.073   0.974   1.008   1.108   1.026   1.056   1.154
195   I        -0.277  -0.391  -0.258  -0.254  -0.467  -0.274  -0.488  -0.704  -0.515
196   P         0.985   1.005   1.073   1.088   1.112   1.199   1.026   1.056   1.154
197   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
198   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
199   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
200   S        -1.268  -1.197  -1.006  -1.365  -1.224  -0.939  -1.447  -1.310  -1.036
201   E         0.140   0.336  -0.147   0.241   0.482  -0.155   0.143   0.403  -0.326
202   T        -1.881  -1.798  -1.584  -1.875  -1.788  -1.444  -1.776  -1.661  -1.347
203   H        -1.201  -1.181  -1.004  -1.022  -1.027  -0.819  -1.372  -1.301  -1.035
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P26786
Output conservation file - output/P26786
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
