1     M         0.816   0.834   0.894   1.075   1.085   1.139   0.846   0.871   0.952
2     S         0.816   0.834   0.894   0.714   0.752   0.851   0.846   0.871   0.952
3     D        -0.284  -0.490  -0.523  -0.255  -0.561  -0.615  -0.341  -0.658  -0.735
4     P        -0.185  -0.431  -0.502   0.085  -0.284  -0.485  -0.200  -0.567  -0.701
5     S        -2.000  -2.209  -1.980  -1.783  -2.070  -1.712  -1.770  -1.988  -1.633
6     S        -0.644  -0.773  -0.549  -0.861  -1.103  -0.732  -0.898  -1.125  -0.779
7     I        -0.185  -0.431  -0.502  -0.082  -0.416  -0.536  -0.200  -0.567  -0.701
8     N         0.816   0.834   0.894   0.927   0.945   1.021   0.846   0.871   0.952
9     G         0.816   0.834   0.894   0.642   0.676   0.793   0.846   0.871   0.952
10    G         0.816   0.834   0.894   0.642   0.676   0.793   0.846   0.871   0.952
11    I        -1.635  -1.491  -1.368  -1.906  -1.676  -1.462  -1.770  -1.571  -1.327
12    V        -1.818  -1.744  -1.389  -2.000  -1.860  -1.332  -1.944  -1.845  -1.350
13    V         0.816   0.834   0.894   0.738   0.762   0.870   0.846   0.871   0.952
14    A         0.816   0.834   0.894   0.786   0.817   0.908   0.846   0.871   0.952
15    M         0.816   0.834   0.894   1.079   1.118   1.142   0.846   0.871   0.952
16    T        -2.409  -2.246  -1.975  -2.538  -2.315  -1.895  -2.225  -2.043  -1.630
17    G         0.816   0.834   0.894   0.643   0.703   0.794   0.846   0.871   0.952
18    K         0.816   0.834   0.894   0.829   0.839   0.943   0.846   0.871   0.952
19    D        -0.103  -0.291  -0.487  -0.028  -0.275  -0.561  -0.088  -0.356  -0.676
20    C         0.816   0.834   0.894   1.047   1.056   1.116   0.846   0.871   0.952
21    V        -0.557  -0.432  -0.512  -0.709  -0.510  -0.675  -0.756  -0.568  -0.718
22    A         0.816   0.834   0.894   0.783   0.830   0.906   0.846   0.871   0.952
23    I         0.816   0.834   0.894   0.796   0.844   0.916   0.846   0.871   0.952
24    A        -0.103   0.044  -0.470  -0.035   0.169  -0.626  -0.088   0.105  -0.649
25    C        -0.557  -0.617  -0.538  -0.426  -0.554  -0.554  -0.756  -0.863  -0.761
26    D         0.816   0.834   0.894   0.750   0.769   0.879   0.846   0.871   0.952
27    L         0.816   0.834   0.894   0.548   0.549   0.718   0.846   0.871   0.952
28    R         0.816   0.834   0.894   0.949   0.957   1.039   0.846   0.871   0.952
29    L        -0.103  -0.204  -0.484  -0.206  -0.347  -0.629  -0.088  -0.230  -0.671
30    G         0.816   0.834   0.894   0.643   0.703   0.794   0.846   0.871   0.952
31    S        -2.832  -2.807  -2.444  -2.746  -2.720  -2.216  -2.359  -2.294  -1.809
32    Q         0.816   0.834   0.894   1.035   1.042   1.107   0.846   0.871   0.952
33    S        -0.980  -1.120  -1.341  -0.882  -1.021  -1.470  -0.890  -1.061  -1.295
34    L        -0.103  -0.291  -0.487  -0.249  -0.520  -0.761  -0.088  -0.356  -0.676
35    G        -0.980  -1.197  -1.347  -0.940  -1.213  -1.466  -0.890  -1.159  -1.303
36    V        -0.557  -0.593  -0.537  -0.789  -0.884  -0.848  -0.756  -0.823  -0.758
37    S        -0.980  -1.029  -1.337  -0.846  -0.879  -1.373  -0.890  -0.967  -1.290
38    N         0.816   0.834   0.894   0.929   0.967   1.022   0.846   0.871   0.952
39    K        -0.980  -0.916  -1.333  -0.657  -0.595  -1.265  -0.890  -0.823  -1.285
40    F         0.816   0.834   0.894   0.813   0.818   0.930   0.846   0.871   0.952
41    E        -0.557  -0.666  -0.541  -0.551  -0.749  -0.564  -0.756  -0.944  -0.765
42    K         0.816   0.834   0.894   0.829   0.839   0.943   0.846   0.871   0.952
43    I        -0.799  -0.794  -0.550  -1.114  -1.120  -0.739  -1.157  -1.160  -0.779
44    F         0.816   0.834   0.894   0.813   0.818   0.930   0.846   0.871   0.952
45    H        -2.991  -2.918  -2.449  -2.579  -2.517  -1.942  -2.492  -2.388  -1.812
46    Y        -1.805  -1.804  -1.959  -1.375  -1.399  -1.754  -1.557  -1.562  -1.615
47    G         0.816   0.834   0.894   0.643   0.703   0.794   0.846   0.871   0.952
48    -         0.816   0.834   0.894   0.741   0.758   0.872   0.846   0.871   0.952
49    H        -1.635  -1.608  -1.376  -1.511  -1.502  -1.171  -1.770  -1.694  -1.337
50    V        -0.799  -0.772  -0.548  -1.100  -1.082  -0.700  -1.157  -1.123  -0.777
51    F        -0.557  -0.690  -0.545  -0.570  -0.824  -0.580  -0.756  -0.983  -0.771
52    L        -0.103   0.094  -0.466  -0.247  -0.044  -0.721  -0.088   0.168  -0.643
53    G         0.816   0.834   0.894   0.643   0.703   0.794   0.846   0.871   0.952
54    I        -0.557  -0.322  -0.490  -0.889  -0.565  -0.767  -0.756  -0.402  -0.682
55    T         0.816   0.834   0.894   0.863   0.864   0.970   0.846   0.871   0.952
56    G         0.816   0.834   0.894   0.643   0.703   0.794   0.846   0.871   0.952
57    L         0.816   0.834   0.894   0.548   0.549   0.718   0.846   0.871   0.952
58    A         0.816   0.834   0.894   0.783   0.830   0.906   0.846   0.871   0.952
59    T         0.816   0.834   0.894   0.863   0.864   0.970   0.846   0.871   0.952
60    D         0.816   0.834   0.894   0.750   0.769   0.879   0.846   0.871   0.952
61    V         0.816   0.834   0.894   0.733   0.768   0.866   0.846   0.871   0.952
62    T        -2.991  -2.808  -2.447  -2.904  -2.613  -2.193  -2.492  -2.304  -1.811
63    T        -0.103  -0.029  -0.474   0.057   0.131  -0.543  -0.088   0.010  -0.656
64    L        -0.103  -0.146  -0.477  -0.259  -0.339  -0.773  -0.088  -0.148  -0.661
65    N        -2.832  -2.821  -2.442  -2.560  -2.480  -1.973  -2.359  -2.304  -1.807
66    E        -0.103  -0.146  -0.477   0.030  -0.037  -0.561  -0.088  -0.148  -0.661
67    M        -2.568  -2.315  -2.421  -2.490  -2.215  -2.241  -2.091  -1.843  -1.791
68    F         0.816   0.834   0.894   0.813   0.818   0.930   0.846   0.871   0.952
69    R         0.816   0.834   0.894   0.949   0.957   1.039   0.846   0.871   0.952
70    Y        -1.382  -1.365  -1.359  -1.200  -1.191  -1.253  -1.424  -1.376  -1.317
71    K         0.816   0.834   0.894   0.829   0.839   0.943   0.846   0.871   0.952
72    T        -1.382  -1.365  -1.368  -1.328  -1.412  -1.347  -1.424  -1.443  -1.328
73    N         0.816   0.834   0.894   0.929   0.967   1.022   0.846   0.871   0.952
74    L        -0.103  -0.146  -0.477  -0.206  -0.276  -0.621  -0.088  -0.148  -0.661
75    Y         0.816   0.834   0.894   0.986   0.998   1.068   0.846   0.871   0.952
76    K        -0.103  -0.146  -0.477   0.055  -0.017  -0.481  -0.088  -0.148  -0.661
77    L        -0.980  -1.247  -1.352  -0.983  -1.370  -1.402  -0.890  -1.220  -1.307
78    K        -0.980  -0.916  -1.333  -0.632  -0.592  -1.239  -0.890  -0.823  -1.285
79    E         0.816   0.834   0.894   0.833   0.851   0.946   0.846   0.871   0.952
80    E        -0.557  -0.347  -0.495  -0.626  -0.319  -0.594  -0.756  -0.438  -0.689
81    R         0.816   0.834   0.894   0.949   0.957   1.039   0.846   0.871   0.952
82    A        -2.991  -2.737  -2.445  -2.663  -2.337  -1.974  -2.492  -2.262  -1.810
83    I         0.816   0.834   0.894   0.796   0.844   0.916   0.846   0.871   0.952
84    E        -0.980  -1.197  -1.347  -0.712  -1.015  -1.254  -0.890  -1.159  -1.303
85    P         0.816   0.834   0.894   0.982   1.011   1.065   0.846   0.871   0.952
86    E        -2.568  -2.587  -2.443  -2.222  -2.308  -2.127  -2.091  -2.099  -1.808
87    T         0.816   0.834   0.894   0.863   0.864   0.970   0.846   0.871   0.952
88    F        -0.557  -0.432  -0.512  -0.710  -0.544  -0.735  -0.756  -0.568  -0.718
89    T        -0.799  -0.605  -0.515  -1.056  -0.727  -0.615  -1.157  -0.843  -0.722
90    Q        -0.799  -0.605  -0.515  -0.839  -0.530  -0.438  -1.157  -0.843  -0.722
91    L         0.816   0.834   0.894   0.548   0.549   0.718   0.846   0.871   0.952
92    V         0.816   0.834   0.894   0.733   0.768   0.866   0.846   0.871   0.952
93    S         0.816   0.834   0.894   0.728   0.816   0.862   0.846   0.871   0.952
94    S        -0.103   0.044  -0.470  -0.058   0.174  -0.576  -0.088   0.105  -0.649
95    S        -0.876  -0.808  -0.548  -1.234  -1.104  -0.696  -1.290  -1.185  -0.776
96    L         0.816   0.834   0.894   0.548   0.549   0.718   0.846   0.871   0.952
97    Y         0.816   0.834   0.894   0.986   0.998   1.068   0.846   0.871   0.952
98    E         0.816   0.834   0.894   0.833   0.851   0.946   0.846   0.871   0.952
99    R        -0.876  -0.804  -0.547  -1.097  -1.024  -0.593  -1.290  -1.178  -0.775
100   R         0.816   0.834   0.894   0.949   0.957   1.039   0.846   0.871   0.952
101   F         0.816   0.834   0.894   0.813   0.818   0.930   0.846   0.871   0.952
102   G         0.816   0.834   0.894   0.643   0.703   0.794   0.846   0.871   0.952
103   P         0.816   0.834   0.894   0.982   1.011   1.065   0.846   0.871   0.952
104   Y        -0.103  -0.029  -0.474   0.241   0.312  -0.279  -0.088   0.010  -0.656
105   F         0.816   0.834   0.894   0.813   0.818   0.930   0.846   0.871   0.952
106   V        -0.103  -0.291  -0.487  -0.074  -0.308  -0.659  -0.088  -0.356  -0.676
107   G        -1.382  -1.512  -1.371  -1.562  -1.714  -1.526  -1.424  -1.562  -1.331
108   P         0.816   0.834   0.894   0.982   1.011   1.065   0.846   0.871   0.952
109   V        -0.799  -0.749  -0.546  -1.136  -1.050  -0.734  -1.157  -1.083  -0.773
110   V         0.816   0.834   0.894   0.733   0.768   0.866   0.846   0.871   0.952
111   A         0.816   0.834   0.894   0.783   0.830   0.906   0.846   0.871   0.952
112   G         0.816   0.834   0.894   0.643   0.703   0.794   0.846   0.871   0.952
113   I        -1.562  -1.632  -1.378  -1.899  -2.002  -1.581  -1.691  -1.729  -1.339
114   N        -1.517  -1.506  -1.380  -1.518  -1.518  -1.401  -1.595  -1.579  -1.341
115   S        -0.103  -0.146  -0.477  -0.041  -0.030  -0.538  -0.088  -0.148  -0.661
116   K        -0.103  -0.291  -0.487   0.052  -0.205  -0.505  -0.088  -0.356  -0.676
117   S        -1.826  -1.751  -1.386  -2.123  -2.004  -1.451  -1.958  -1.852  -1.348
118   G        -1.562  -1.592  -1.375  -1.765  -1.758  -1.409  -1.691  -1.694  -1.336
119   K        -0.980  -1.197  -1.347  -0.737  -1.070  -1.306  -0.890  -1.159  -1.303
120   P         0.816   0.834   0.894   0.982   1.011   1.065   0.846   0.871   0.952
121   F         0.816   0.834   0.894   0.813   0.818   0.930   0.846   0.871   0.952
122   I         0.816   0.834   0.894   0.796   0.844   0.916   0.846   0.871   0.952
123   A        -0.799  -0.605  -0.515  -0.865  -0.527  -0.474  -1.157  -0.843  -0.722
124   G         0.816   0.834   0.894   0.643   0.703   0.794   0.846   0.871   0.952
125   F         0.816   0.834   0.894   0.813   0.818   0.930   0.846   0.871   0.952
126   D         0.816   0.834   0.894   0.750   0.769   0.879   0.846   0.871   0.952
127   L        -0.799  -0.800  -0.550  -1.338  -1.342  -0.890  -1.157  -1.170  -0.779
128   I         0.816   0.834   0.894   0.796   0.844   0.916   0.846   0.871   0.952
129   G         0.816   0.834   0.894   0.643   0.703   0.794   0.846   0.871   0.952
130   C         0.816   0.834   0.894   1.047   1.056   1.116   0.846   0.871   0.952
131   I         0.816   0.834   0.894   0.796   0.844   0.916   0.846   0.871   0.952
132   D         0.816   0.834   0.894   0.750   0.769   0.879   0.846   0.871   0.952
133   E        -0.799  -0.605  -0.515  -1.045  -0.743  -0.609  -1.157  -0.843  -0.722
134   A        -0.103  -0.029  -0.474  -0.023   0.096  -0.600  -0.088   0.010  -0.656
135   K        -0.980  -1.095  -1.343  -0.683  -0.879  -1.171  -0.890  -1.040  -1.297
136   D         0.816   0.834   0.894   0.750   0.769   0.879   0.846   0.871   0.952
137   F         0.816   0.834   0.894   0.813   0.818   0.930   0.846   0.871   0.952
138   I         0.816   0.834   0.894   0.796   0.844   0.916   0.846   0.871   0.952
139   V         0.816   0.834   0.894   0.733   0.768   0.866   0.846   0.871   0.952
140   S         0.816   0.834   0.894   0.728   0.816   0.862   0.846   0.871   0.952
141   G         0.816   0.834   0.894   0.643   0.703   0.794   0.846   0.871   0.952
142   T         0.816   0.834   0.894   0.863   0.864   0.970   0.846   0.871   0.952
143   A         0.816   0.834   0.894   0.783   0.830   0.906   0.846   0.871   0.952
144   S        -0.557  -0.666  -0.541  -0.691  -0.846  -0.693  -0.756  -0.944  -0.765
145   D        -0.557  -0.666  -0.541  -0.680  -0.896  -0.695  -0.756  -0.944  -0.765
146   Q        -0.103  -0.204  -0.484   0.277   0.138  -0.297  -0.088  -0.230  -0.671
147   L         0.816   0.834   0.894   0.548   0.549   0.718   0.846   0.871   0.952
148   F        -0.876  -0.796  -0.545  -1.083  -0.969  -0.566  -1.290  -1.165  -0.771
149   G         0.816   0.834   0.894   0.643   0.703   0.794   0.846   0.871   0.952
150   M         0.816   0.834   0.894   1.079   1.118   1.142   0.846   0.871   0.952
151   C         0.816   0.834   0.894   1.047   1.056   1.116   0.846   0.871   0.952
152   E         0.816   0.834   0.894   0.833   0.851   0.946   0.846   0.871   0.952
153   S        -0.103  -0.146  -0.477  -0.049  -0.037  -0.560  -0.088  -0.148  -0.661
154   L        -0.103  -0.291  -0.487  -0.259  -0.538  -0.789  -0.088  -0.356  -0.676
155   Y        -0.557  -0.623  -0.539  -0.347  -0.476  -0.373  -0.756  -0.873  -0.762
156   E        -0.103  -0.204  -0.484   0.071  -0.064  -0.453  -0.088  -0.230  -0.671
157   P         0.816   0.834   0.894   0.982   1.011   1.065   0.846   0.871   0.952
158   N        -0.103  -0.204  -0.484   0.125   0.000  -0.505  -0.088  -0.230  -0.671
159   L         0.816   0.834   0.894   0.548   0.549   0.718   0.846   0.871   0.952
160   E        -0.103  -0.146  -0.477   0.015  -0.047  -0.604  -0.088  -0.148  -0.661
161   P        -0.557  -0.347  -0.495  -0.472  -0.147  -0.476  -0.756  -0.438  -0.689
162   E        -0.557  -0.586  -0.539  -0.626  -0.710  -0.683  -0.756  -0.812  -0.762
163   D         0.816   0.834   0.894   0.750   0.769   0.879   0.846   0.871   0.952
164   L         0.816   0.834   0.894   0.548   0.549   0.718   0.846   0.871   0.952
165   F         0.816   0.834   0.894   0.813   0.818   0.930   0.846   0.871   0.952
166   E        -0.103  -0.204  -0.484   0.056  -0.078  -0.497  -0.088  -0.230  -0.671
167   T         0.816   0.834   0.894   0.863   0.864   0.970   0.846   0.871   0.952
168   I        -0.103  -0.204  -0.484   0.036  -0.068  -0.473  -0.088  -0.230  -0.671
169   S         0.816   0.834   0.894   0.728   0.816   0.862   0.846   0.871   0.952
170   Q         0.816   0.834   0.894   1.035   1.042   1.107   0.846   0.871   0.952
171   A        -0.103   0.029  -0.472  -0.023   0.165  -0.595  -0.088   0.086  -0.652
172   L         0.816   0.834   0.894   0.548   0.549   0.718   0.846   0.871   0.952
173   L        -0.103  -0.204  -0.484  -0.206  -0.347  -0.629  -0.088  -0.230  -0.671
174   N        -0.103  -0.204  -0.484   0.167   0.053  -0.382  -0.088  -0.230  -0.671
175   A         0.816   0.834   0.894   0.783   0.830   0.906   0.846   0.871   0.952
176   A        -2.249  -2.327  -1.981  -2.230  -2.266  -1.723  -2.091  -2.112  -1.635
177   D         0.816   0.834   0.894   0.750   0.769   0.879   0.846   0.871   0.952
178   R         0.816   0.834   0.894   0.949   0.957   1.039   0.846   0.871   0.952
179   D         0.816   0.834   0.894   0.750   0.769   0.879   0.846   0.871   0.952
180   A        -0.980  -1.247  -1.352  -0.726  -1.060  -1.206  -0.890  -1.220  -1.307
181   L        -0.103  -0.291  -0.487  -0.249  -0.520  -0.761  -0.088  -0.356  -0.676
182   S        -0.103  -0.204  -0.484  -0.090  -0.161  -0.693  -0.088  -0.230  -0.671
183   G         0.816   0.834   0.894   0.643   0.703   0.794   0.846   0.871   0.952
184   W         0.816   0.834   0.894   1.167   1.178   1.212   0.846   0.871   0.952
185   G         0.816   0.834   0.894   0.643   0.703   0.794   0.846   0.871   0.952
186   A        -0.103  -0.291  -0.487   0.023  -0.194  -0.486  -0.088  -0.356  -0.676
187   V        -0.980  -1.120  -1.341  -0.784  -0.959  -1.242  -0.890  -1.061  -1.295
188   V         0.816   0.834   0.894   0.733   0.768   0.866   0.846   0.871   0.952
189   Y        -0.103  -0.204  -0.484   0.241   0.107  -0.293  -0.088  -0.230  -0.671
190   I        -0.103  -0.291  -0.487  -0.010  -0.244  -0.612  -0.088  -0.356  -0.676
191   I        -0.557  -0.432  -0.512  -0.668  -0.457  -0.662  -0.756  -0.568  -0.718
192   K        -1.562  -1.455  -1.359  -1.648  -1.484  -1.319  -1.691  -1.513  -1.316
193   K        -0.103  -0.291  -0.487   0.031  -0.236  -0.567  -0.088  -0.356  -0.676
194   D         0.816   0.834   0.894   0.750   0.769   0.879   0.846   0.871   0.952
195   E        -0.980  -0.697  -1.320  -0.726  -0.405  -1.238  -0.890  -0.548  -1.271
196   V        -0.103  -0.291  -0.487  -0.027  -0.254  -0.521  -0.088  -0.356  -0.676
197   V        -1.826  -1.736  -1.389  -2.115  -1.981  -1.438  -1.958  -1.838  -1.350
198   K         0.816   0.834   0.894   0.829   0.839   0.943   0.846   0.871   0.952
199   R        -0.557  -0.533  -0.532  -0.489  -0.492  -0.552  -0.756  -0.726  -0.750
200   Y        -1.826  -1.656  -1.375  -2.166  -1.999  -1.503  -1.958  -1.738  -1.335
201   L        -0.103  -0.291  -0.487  -0.259  -0.538  -0.789  -0.088  -0.356  -0.676
202   K         0.816   0.834   0.894   0.829   0.839   0.943   0.846   0.871   0.952
203   M        -1.722  -1.621  -1.375  -1.795  -1.727  -1.337  -1.824  -1.706  -1.336
204   R         0.816   0.834   0.894   0.949   0.957   1.039   0.846   0.871   0.952
205   Q         0.816   0.834   0.894   1.035   1.042   1.107   0.846   0.871   0.952
206   D         0.816   0.834   0.894   0.750   0.769   0.879   0.846   0.871   0.952
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P25451
Output conservation file - output/P25451
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
