1     M         1.895   1.945   2.056   2.107   2.203   2.359   2.097   2.171   2.358
2     S        -0.178  -0.380  -0.416  -0.010  -0.350  -0.496  -0.159  -0.414  -0.460
3     Y        -2.121  -2.041  -1.923  -1.700  -1.613  -1.437  -1.411  -1.334  -1.182
4     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
5     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
6     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
7     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
8     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
9     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
10    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
11    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
12    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
13    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
14    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
15    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
16    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
17    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
18    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
19    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
20    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
21    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
22    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
23    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
24    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
25    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
26    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
27    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
28    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
29    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
30    -        -0.504  -0.508  -0.485  -0.259  -0.271  -0.257  -0.576  -0.579  -0.546
31    -        -1.119  -1.070  -0.974  -0.939  -0.894  -0.723  -1.022  -0.960  -0.832
32    K         1.168   1.163   1.018   0.767   0.812   0.586   1.220   1.206   0.912
33    Q        -0.529  -0.437  -0.482  -0.448  -0.352  -0.358  -0.660  -0.553  -0.558
34    Y        -0.529  -0.543  -0.496  -0.407  -0.417  -0.332  -0.660  -0.644  -0.569
35    F         0.475   0.285   0.217   0.718   0.455   0.336   0.468   0.185   0.060
36    D        -0.990  -0.925  -0.932  -0.839  -0.771  -0.730  -0.910  -0.837  -0.798
37    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
38    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
39    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
40    S        -0.990  -0.911  -0.934  -1.329  -1.210  -1.203  -0.910  -0.829  -0.799
41    L         1.895   1.945   2.056   1.784   1.842   2.037   2.097   2.171   2.358
42    P         1.895   1.945   2.056   1.853   1.906   2.106   2.097   2.171   2.358
43    L        -1.325  -1.243  -1.288  -1.097  -0.972  -1.132  -1.036  -0.954  -0.944
44    K         0.157   0.097   0.098  -0.280  -0.321  -0.321  -0.033  -0.119  -0.137
45    L         1.895   1.945   2.056   1.784   1.842   2.037   2.097   2.171   2.358
46    K        -0.990  -1.092  -0.972  -1.156  -1.190  -1.022  -0.910  -0.984  -0.833
47    S        -0.447  -0.389  -0.453  -0.220  -0.146  -0.218  -0.534  -0.455  -0.517
48    F         1.895   1.945   2.056   1.988   2.084   2.240   2.097   2.171   2.358
49    F         1.168   1.129   1.024   1.325   1.291   1.109   1.220   1.149   0.924
50    Q        -1.117  -1.064  -0.978  -1.191  -1.145  -1.008  -1.036  -0.970  -0.841
51    R         0.618   0.647   0.746  -0.052   0.019   0.154   0.217   0.249   0.370
52    Y         0.618   0.623   0.735   0.558   0.568   0.699   0.217   0.201   0.347
53    P         1.895   1.945   2.056   1.853   1.906   2.106   2.097   2.171   2.358
54    P         1.895   1.945   2.056   1.853   1.906   2.106   2.097   2.171   2.358
55    S        -1.451  -1.451  -1.343  -1.235  -1.268  -1.083  -1.161  -1.137  -0.992
56    -         0.060   0.015   0.068  -0.031  -0.135  -0.039  -0.148  -0.221  -0.169
57    -         0.060   0.015   0.068   0.097   0.003   0.061  -0.148  -0.221  -0.169
58    I        -0.990  -1.026  -0.951  -0.741  -0.837  -0.705  -0.910  -0.928  -0.813
59    K        -1.325  -1.410  -1.325  -1.593  -1.680  -1.513  -1.036  -1.097  -0.976
60    Y         0.475   0.285   0.217   0.822   0.605   0.471   0.468   0.185   0.060
61    S        -0.782  -0.855  -0.869  -0.542  -0.655  -0.739  -0.660  -0.739  -0.725
62    P        -1.451  -1.360  -1.317  -1.104  -1.019  -0.919  -1.161  -1.069  -0.973
63    V        -0.782  -0.867  -0.883  -0.922  -0.995  -0.964  -0.660  -0.751  -0.740
64    S         0.475   0.549   0.253   0.503   0.570   0.117   0.468   0.572   0.115
65    T        -0.990  -1.092  -0.972  -0.727  -0.886  -0.712  -0.910  -0.984  -0.833
66    S        -0.571  -0.467  -0.507  -0.458  -0.293  -0.388  -0.685  -0.547  -0.577
67    T        -0.571  -0.618  -0.509  -0.479  -0.573  -0.441  -0.685  -0.715  -0.583
68    K        -1.098  -1.040  -0.971  -1.167  -1.113  -0.943  -1.013  -0.941  -0.833
69    A         0.050  -0.012   0.052   0.111   0.007   0.127  -0.194  -0.272  -0.199
70    I         0.050   0.228   0.099   0.046   0.269   0.139  -0.194   0.075  -0.127
71    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
72    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
73    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
74    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
75    N        -1.451  -1.461  -1.335  -1.358  -1.397  -1.268  -1.161  -1.142  -0.985
76    A        -0.782  -0.881  -0.900  -0.490  -0.675  -0.679  -0.660  -0.764  -0.758
77    N         1.895   1.945   2.056   1.988   2.067   2.240   2.097   2.171   2.358
78    P         1.895   1.945   2.056   1.853   1.906   2.106   2.097   2.171   2.358
79    F         1.895   1.945   2.056   1.988   2.084   2.240   2.097   2.171   2.358
80    L         0.475   0.426   0.245   0.549   0.475   0.253   0.468   0.396   0.104
81    P         0.618   0.653   0.747   0.358   0.368   0.533   0.217   0.261   0.371
82    N         0.809   0.721   0.792   0.865   0.701   0.800   0.593   0.398   0.466
83    K         1.168   1.039   1.010   0.758   0.632   0.555   1.220   0.995   0.898
84    H         0.014   0.034   0.071   0.067   0.100   0.169  -0.284  -0.239  -0.186
85    P        -0.178  -0.138  -0.380   0.019   0.040  -0.256  -0.159  -0.102  -0.415
86    V        -0.990  -1.003  -0.945  -0.711  -0.719  -0.640  -0.910  -0.910  -0.807
87    T         0.475   0.346   0.231   0.806   0.628   0.454   0.468   0.272   0.082
88    Q         0.475   0.549   0.253   0.844   0.953   0.536   0.468   0.572   0.115
89    R        -0.447  -0.482  -0.466  -0.921  -0.965  -0.924  -0.534  -0.552  -0.532
90    F        -0.990  -0.965  -0.935  -0.550  -0.463  -0.468  -0.910  -0.860  -0.797
91    H         0.157   0.080   0.105   0.418   0.329   0.342  -0.033  -0.127  -0.119
92    D        -1.451  -1.327  -1.315  -1.283  -1.133  -1.124  -1.161  -1.034  -0.971
93    P         0.618   0.706   0.768   0.358   0.467   0.572   0.217   0.369   0.415
94    K         0.475   0.314   0.217   0.117  -0.056  -0.243   0.468   0.228   0.061
95    Y         1.168   1.199   1.024   1.420   1.503   1.232   1.220   1.264   0.925
96    S         1.895   1.945   2.056   1.997   2.093   2.249   2.097   2.171   2.358
97    L        -0.178  -0.206  -0.358  -0.096  -0.181  -0.363  -0.159  -0.204  -0.386
98    R         1.895   1.945   2.056   1.930   2.013   2.182   2.097   2.171   2.358
99    R         1.895   1.945   2.056   1.930   2.013   2.182   2.097   2.171   2.358
100   M        -0.195  -0.106  -0.007  -0.160  -0.014   0.110  -0.534  -0.405  -0.283
101   S         0.557   0.617   0.737   0.354   0.439   0.623   0.092   0.188   0.351
102   D         0.809   0.721   0.792   0.934   0.758   0.822   0.593   0.398   0.466
103   V         0.157   0.225   0.117   0.127   0.180   0.095  -0.033   0.039  -0.110
104   Y         0.157   0.152   0.124   0.339   0.377   0.294  -0.033  -0.027  -0.090
105   K         1.895   1.945   2.056   1.403   1.523   1.658   2.097   2.171   2.358
106   L         0.475   0.417   0.243   0.530   0.415   0.215   0.468   0.370   0.100
107   A         1.895   1.945   2.056   1.943   2.014   2.196   2.097   2.171   2.358
108   L        -1.451  -1.453  -1.332  -1.293  -1.303  -1.183  -1.161  -1.137  -0.983
109   R        -0.990  -0.970  -0.939  -1.419  -1.421  -1.325  -0.910  -0.871  -0.802
110   Y        -0.656  -0.705  -0.559  -0.486  -0.525  -0.335  -0.785  -0.817  -0.639
111   G         1.168   1.039   1.010   1.454   1.302   1.243   1.220   0.995   0.898
112   V         0.014  -0.036   0.063   0.111   0.047   0.173  -0.284  -0.319  -0.195
113   E        -0.656  -0.652  -0.539  -0.503  -0.476  -0.335  -0.785  -0.754  -0.616
114   E         0.014  -0.064   0.050   0.033  -0.083   0.080  -0.284  -0.367  -0.216
115   F         1.168   1.299   1.035   1.150   1.302   0.987   1.220   1.422   0.945
116   L         1.895   1.945   2.056   1.784   1.842   2.037   2.097   2.171   2.358
117   P         1.895   1.945   2.056   1.853   1.906   2.106   2.097   2.171   2.358
118   P         0.357   0.342   0.185   0.463   0.407   0.221   0.297   0.262   0.011
119   I        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
120   E        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
121   N        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
122   T        -0.333  -0.238  -0.414  -0.018   0.109  -0.168  -0.358  -0.235  -0.456
123   K        -1.119  -1.107  -1.001  -1.522  -1.505  -1.276  -1.022  -0.994  -0.858
124   K         0.157   0.019   0.086  -0.312  -0.455  -0.370  -0.033  -0.227  -0.156
125   L        -0.656  -0.609  -0.536  -1.110  -1.005  -0.875  -0.785  -0.714  -0.614
126   F         0.475   0.285   0.217   0.717   0.467   0.334   0.468   0.185   0.060
127   F        -0.782  -0.881  -0.900  -0.405  -0.551  -0.578  -0.660  -0.764  -0.758
128   E         1.168   1.039   1.010   1.276   1.145   1.104   1.220   0.995   0.898
129   E        -0.178  -0.328  -0.392   0.078  -0.116  -0.193  -0.159  -0.350  -0.429
130   K         1.168   1.163   1.018   0.758   0.803   0.567   1.220   1.206   0.912
131   Y        -0.178  -0.380  -0.416   0.200  -0.072  -0.162  -0.159  -0.414  -0.460
132   N        -0.990  -1.074  -0.942  -0.840  -0.969  -0.774  -0.910  -0.967  -0.807
133   K        -0.990  -1.030  -0.943  -1.015  -1.082  -0.942  -0.910  -0.932  -0.808
134   K         0.157   0.056   0.071  -0.340  -0.464  -0.436  -0.033  -0.201  -0.178
135   T        -1.325  -1.344  -1.301  -1.395  -1.349  -1.253  -1.036  -1.042  -0.955
136   L        -1.451  -1.464  -1.347  -1.160  -1.176  -1.054  -1.161  -1.145  -0.995
137   M         0.557   0.636   0.746   0.321   0.395   0.582   0.092   0.227   0.369
138   K        -0.571  -0.533  -0.506  -0.941  -0.880  -0.775  -0.685  -0.616  -0.579
139   G         1.895   1.945   2.056   2.089   2.183   2.342   2.097   2.171   2.358
140   V        -0.990  -0.955  -0.935  -0.766  -0.806  -0.709  -0.910  -0.869  -0.801
141   L        -0.178  -0.259  -0.382  -0.041  -0.174  -0.306  -0.159  -0.257  -0.416
142   L        -0.990  -0.788  -0.922  -0.726  -0.483  -0.627  -0.910  -0.713  -0.788
143   P        -0.178  -0.195  -0.365  -0.060  -0.125  -0.365  -0.159  -0.178  -0.395
144   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
145   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
146   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
147   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
148   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
149   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
150   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
151   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
152   K         1.168   1.199   1.024   0.760   0.849   0.581   1.220   1.264   0.925
153   G         0.157   0.191   0.099   0.144   0.218  -0.051  -0.033   0.019  -0.135
154   H         0.809   0.750   0.792   0.784   0.633   0.578   0.593   0.457   0.465
155   K         0.157   0.093   0.085  -0.269  -0.317  -0.313  -0.033  -0.151  -0.161
156   H        -0.782  -0.859  -0.901  -0.428  -0.566  -0.694  -0.660  -0.740  -0.759
157   E         1.168   1.199   1.024   1.258   1.322   1.089   1.220   1.264   0.925
158   L        -0.447  -0.380  -0.427  -0.598  -0.481  -0.636  -0.534  -0.423  -0.481
159   K        -1.117  -1.017  -0.970  -1.373  -1.255  -1.188  -1.036  -0.931  -0.835
160   L        -0.178  -0.235  -0.374  -0.177  -0.319  -0.527  -0.159  -0.240  -0.406
161   N        -0.990  -0.940  -0.935  -0.806  -0.726  -0.707  -0.910  -0.857  -0.802
162   E        -0.447  -0.386  -0.452  -0.388  -0.336  -0.386  -0.534  -0.472  -0.518
163   K         0.157   0.079   0.104  -0.308  -0.358  -0.343  -0.033  -0.129  -0.126
164   L        -0.990  -0.863  -0.900  -0.979  -0.861  -0.858  -0.910  -0.773  -0.767
165   K         0.014   0.124   0.067  -0.578  -0.377  -0.431  -0.284  -0.169  -0.196
166   K        -0.782  -0.754  -0.870  -1.031  -0.979  -1.112  -0.660  -0.629  -0.726
167   R        -0.447  -0.280  -0.422  -0.554  -0.251  -0.517  -0.534  -0.313  -0.478
168   E        -0.195  -0.179  -0.038  -0.426  -0.420  -0.201  -0.534  -0.509  -0.328
169   E        -0.178  -0.265  -0.382  -0.143  -0.317  -0.564  -0.159  -0.275  -0.416
170   A         0.475   0.452   0.250   0.611   0.520   0.231   0.468   0.415   0.111
171   L         0.014  -0.014   0.061  -0.024  -0.100   0.037  -0.284  -0.311  -0.203
172   K        -1.117  -1.105  -0.974  -1.452  -1.371  -1.214  -1.036  -0.996  -0.837
173   K        -0.990  -0.993  -0.937  -0.993  -1.009  -0.975  -0.910  -0.902  -0.803
174   V        -0.447  -0.376  -0.440  -0.183  -0.057  -0.163  -0.534  -0.426  -0.495
175   D        -0.178  -0.261  -0.375   0.169  -0.001  -0.200  -0.159  -0.275  -0.408
176   E        -0.529  -0.543  -0.482  -0.921  -0.904  -0.787  -0.660  -0.653  -0.557
177   L        -0.571  -0.524  -0.518  -0.620  -0.624  -0.625  -0.685  -0.631  -0.592
178   I         1.103   1.221   0.989   1.367   1.532   1.178   1.115   1.299   0.859
179   A        -0.954  -0.930  -0.936  -0.749  -0.752  -0.739  -0.849  -0.817  -0.794
180   S         0.050   0.145   0.074  -0.035   0.092  -0.002  -0.194  -0.067  -0.165
181   K        -1.098  -1.048  -0.982  -1.013  -0.910  -0.874  -1.013  -0.950  -0.842
182   K         0.809   0.882   0.860   0.251   0.395   0.315   0.593   0.710   0.604
183   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
184   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
185   G         1.025   0.910   0.925   1.269   1.117   1.115   0.983   0.762   0.733
186   S        -0.113  -0.098  -0.008  -0.445  -0.472  -0.401  -0.409  -0.392  -0.284
187   K         0.475   0.426   0.245   0.134   0.113  -0.169   0.468   0.396   0.104
188   Y        -0.178  -0.380  -0.416   0.213  -0.056  -0.140  -0.159  -0.414  -0.460
189   A        -0.447  -0.345  -0.453  -0.752  -0.571  -0.700  -0.534  -0.429  -0.519
190   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
191   K        -0.656  -0.637  -0.539  -1.141  -1.061  -0.915  -0.785  -0.747  -0.618
192   R        -0.178  -0.166  -0.368  -0.065  -0.080  -0.441  -0.159  -0.140  -0.399
193   V        -0.529  -0.474  -0.465  -0.477  -0.412  -0.353  -0.660  -0.591  -0.541
194   E        -0.178  -0.291  -0.381   0.067  -0.083  -0.197  -0.159  -0.309  -0.415
195   K         1.168   1.163   1.018   0.758   0.803   0.567   1.220   1.206   0.912
196   M        -0.954  -0.864  -0.929  -0.883  -0.792  -0.916  -0.849  -0.756  -0.788
197   K        -0.571  -0.557  -0.512  -0.737  -0.742  -0.737  -0.685  -0.650  -0.582
198   K        -1.098  -1.090  -0.969  -1.427  -1.392  -1.223  -1.013  -0.988  -0.831
199   N        -1.863  -1.781  -1.667  -1.779  -1.658  -1.516  -1.340  -1.256  -1.110
200   Q        -0.333  -0.313  -0.426  -0.633  -0.582  -0.706  -0.358  -0.348  -0.472
201   S        -0.954  -0.864  -0.929  -1.204  -1.065  -1.096  -0.849  -0.756  -0.788
202   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
203   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
204   I        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
205   G        -1.098  -1.049  -0.973  -0.829  -0.773  -0.655  -1.013  -0.945  -0.835
206   W         1.103   0.944   0.964   1.490   1.280   1.294   1.115   0.826   0.809
207   F        -0.571  -0.597  -0.511  -0.356  -0.393  -0.246  -0.685  -0.706  -0.585
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P23369
Output conservation file - output/P23369
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
