1     M         0.982   0.982   0.982   1.299   1.299   1.299   0.982   0.982   0.982
2     K         0.982   0.982   0.982   0.859   0.859   0.859   0.982   0.982   0.982
3     F        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
4     S        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
5     G        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
6     L        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
7     W        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
8     C        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
9     W        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
10    L        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
11    L        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
12    L        -1.013  -1.013  -1.013  -1.006  -1.006  -1.006  -1.013  -1.013  -1.013
13    F        -1.013  -1.013  -1.013  -1.018  -1.018  -1.018  -1.013  -1.013  -1.013
14    L        -1.013  -1.013  -1.013  -1.054  -1.054  -1.054  -1.013  -1.013  -1.013
15    S         0.982   0.982   0.982   1.009   1.009   1.009   0.982   0.982   0.982
16    V        -1.013  -1.013  -1.013  -1.140  -1.140  -1.140  -1.013  -1.013  -1.013
17    N        -1.013  -1.013  -1.013  -0.817  -0.817  -0.817  -1.013  -1.013  -1.013
18    V        -1.013  -1.013  -1.013  -1.165  -1.165  -1.165  -1.013  -1.013  -1.013
19    I         0.982   0.982   0.982   1.009   1.009   1.009   0.982   0.982   0.982
20    A         0.982   0.982   0.982   1.172   1.172   1.172   0.982   0.982   0.982
21    S         0.982   0.982   0.982   1.009   1.009   1.009   0.982   0.982   0.982
22    D        -1.013  -1.013  -1.013  -1.030  -1.030  -1.030  -1.013  -1.013  -1.013
23    V        -1.013  -1.013  -1.013  -1.140  -1.140  -1.140  -1.013  -1.013  -1.013
24    G        -1.013  -1.013  -1.013  -1.384  -1.384  -1.384  -1.013  -1.013  -1.013
25    E        -1.013  -1.013  -1.013  -0.840  -0.840  -0.840  -1.013  -1.013  -1.013
26    L        -1.013  -1.013  -1.013  -1.018  -1.018  -1.018  -1.013  -1.013  -1.013
27    I        -1.013  -1.013  -1.013  -0.828  -0.828  -0.828  -1.013  -1.013  -1.013
28    D        -1.013  -1.013  -1.013  -1.079  -1.079  -1.079  -1.013  -1.013  -1.013
29    Q         0.982   0.982   0.982   1.251   1.251   1.251   0.982   0.982   0.982
30    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
31    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
32    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
33    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
34    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
35    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
36    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
37    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
38    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
39    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
40    D        -1.013  -1.013  -1.013  -1.140  -1.140  -1.140  -1.013  -1.013  -1.013
41    D        -1.013  -1.013  -1.013  -0.982  -0.982  -0.982  -1.013  -1.013  -1.013
42    E        -1.013  -1.013  -1.013  -0.805  -0.805  -0.805  -1.013  -1.013  -1.013
43    V        -1.013  -1.013  -1.013  -0.982  -0.982  -0.982  -1.013  -1.013  -1.013
44    I        -1.013  -1.013  -1.013  -1.140  -1.140  -1.140  -1.013  -1.013  -1.013
45    T         0.982   0.982   0.982   0.943   0.943   0.943   0.982   0.982   0.982
46    Q        -1.013  -1.013  -1.013  -0.759  -0.759  -0.759  -1.013  -1.013  -1.013
47    K         0.982   0.982   0.982   0.859   0.859   0.859   0.982   0.982   0.982
48    V         0.982   0.982   0.982   0.792   0.792   0.792   0.982   0.982   0.982
49    F        -1.013  -1.013  -1.013  -0.840  -0.840  -0.840  -1.013  -1.013  -1.013
50    F         0.982   0.982   0.982   0.993   0.993   0.993   0.982   0.982   0.982
51    D         0.982   0.982   0.982   0.943   0.943   0.943   0.982   0.982   0.982
52    I        -1.013  -1.013  -1.013  -1.140  -1.140  -1.140  -1.013  -1.013  -1.013
53    E         0.982   0.982   0.982   1.042   1.042   1.042   0.982   0.982   0.982
54    H        -1.013  -1.013  -1.013  -0.771  -0.771  -0.771  -1.013  -1.013  -1.013
55    G        -1.013  -1.013  -1.013  -1.267  -1.267  -1.267  -1.013  -1.013  -1.013
56    E        -1.013  -1.013  -1.013  -1.191  -1.191  -1.191  -1.013  -1.013  -1.013
57    E        -1.013  -1.013  -1.013  -1.066  -1.066  -1.066  -1.013  -1.013  -1.013
58    K        -1.013  -1.013  -1.013  -1.091  -1.091  -1.091  -1.013  -1.013  -1.013
59    V        -1.013  -1.013  -1.013  -1.140  -1.140  -1.140  -1.013  -1.013  -1.013
60    G         0.982   0.982   0.982   0.689   0.689   0.689   0.982   0.982   0.982
61    R         0.982   0.982   0.982   1.204   1.204   1.204   0.982   0.982   0.982
62    I         0.982   0.982   0.982   1.009   1.009   1.009   0.982   0.982   0.982
63    V        -1.013  -1.013  -1.013  -1.191  -1.191  -1.191  -1.013  -1.013  -1.013
64    I         0.982   0.982   0.982   1.009   1.009   1.009   0.982   0.982   0.982
65    G         0.982   0.982   0.982   0.689   0.689   0.689   0.982   0.982   0.982
66    L         0.982   0.982   0.982   0.976   0.976   0.976   0.982   0.982   0.982
67    Y        -1.013  -1.013  -1.013  -0.840  -0.840  -0.840  -1.013  -1.013  -1.013
68    G         0.982   0.982   0.982   0.689   0.689   0.689   0.982   0.982   0.982
69    K        -1.013  -1.013  -1.013  -1.140  -1.140  -1.140  -1.013  -1.013  -1.013
70    V         0.982   0.982   0.982   0.792   0.792   0.792   0.982   0.982   0.982
71    C        -1.013  -1.013  -1.013  -0.887  -0.887  -0.887  -1.013  -1.013  -1.013
72    P         0.982   0.982   0.982   1.219   1.219   1.219   0.982   0.982   0.982
73    K         0.982   0.982   0.982   0.859   0.859   0.859   0.982   0.982   0.982
74    T         0.982   0.982   0.982   0.943   0.943   0.943   0.982   0.982   0.982
75    A        -1.013  -1.013  -1.013  -1.018  -1.018  -1.018  -1.013  -1.013  -1.013
76    K        -1.013  -1.013  -1.013  -1.066  -1.066  -1.066  -1.013  -1.013  -1.013
77    N         0.982   0.982   0.982   1.075   1.075   1.075   0.982   0.982   0.982
78    F         0.982   0.982   0.982   0.993   0.993   0.993   0.982   0.982   0.982
79    Y        -1.013  -1.013  -1.013  -0.691  -0.691  -0.691  -1.013  -1.013  -1.013
80    K        -1.013  -1.013  -1.013  -1.254  -1.254  -1.254  -1.013  -1.013  -1.013
81    L         0.982   0.982   0.982   0.976   0.976   0.976   0.982   0.982   0.982
82    S        -1.013  -1.013  -1.013  -0.736  -0.736  -0.736  -1.013  -1.013  -1.013
83    T         0.982   0.982   0.982   0.943   0.943   0.943   0.982   0.982   0.982
84    T        -1.013  -1.013  -1.013  -1.267  -1.267  -1.267  -1.013  -1.013  -1.013
85    T        -1.013  -1.013  -1.013  -1.006  -1.006  -1.006  -1.013  -1.013  -1.013
86    N        -1.013  -1.013  -1.013  -0.958  -0.958  -0.958  -1.013  -1.013  -1.013
87    S        -1.013  -1.013  -1.013  -1.216  -1.216  -1.216  -1.013  -1.013  -1.013
88    K        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
89    K        -1.013  -1.013  -1.013  -0.934  -0.934  -0.934  -1.013  -1.013  -1.013
90    G        -1.013  -1.013  -1.013  -1.216  -1.216  -1.216  -1.013  -1.013  -1.013
91    F        -1.013  -1.013  -1.013  -0.840  -0.840  -0.840  -1.013  -1.013  -1.013
92    I        -1.013  -1.013  -1.013  -0.958  -0.958  -0.958  -1.013  -1.013  -1.013
93    G        -1.013  -1.013  -1.013  -1.165  -1.165  -1.165  -1.013  -1.013  -1.013
94    S        -1.013  -1.013  -1.013  -1.030  -1.030  -1.030  -1.013  -1.013  -1.013
95    T        -1.013  -1.013  -1.013  -1.191  -1.191  -1.191  -1.013  -1.013  -1.013
96    F         0.982   0.982   0.982   0.993   0.993   0.993   0.982   0.982   0.982
97    H         0.982   0.982   0.982   1.267   1.267   1.267   0.982   0.982   0.982
98    R         0.982   0.982   0.982   1.204   1.204   1.204   0.982   0.982   0.982
99    V         0.982   0.982   0.982   0.792   0.792   0.792   0.982   0.982   0.982
100   I         0.982   0.982   0.982   1.009   1.009   1.009   0.982   0.982   0.982
101   P        -1.013  -1.013  -1.013  -0.934  -0.934  -0.934  -1.013  -1.013  -1.013
102   N        -1.013  -1.013  -1.013  -0.982  -0.982  -0.982  -1.013  -1.013  -1.013
103   F         0.982   0.982   0.982   0.993   0.993   0.993   0.982   0.982   0.982
104   M         0.982   0.982   0.982   1.299   1.299   1.299   0.982   0.982   0.982
105   V        -1.013  -1.013  -1.013  -1.140  -1.140  -1.140  -1.013  -1.013  -1.013
106   Q         0.982   0.982   0.982   1.251   1.251   1.251   0.982   0.982   0.982
107   G        -1.013  -1.013  -1.013  -1.216  -1.216  -1.216  -1.013  -1.013  -1.013
108   G         0.982   0.982   0.982   0.689   0.689   0.689   0.982   0.982   0.982
109   D         0.982   0.982   0.982   0.943   0.943   0.943   0.982   0.982   0.982
110   F         0.982   0.982   0.982   0.993   0.993   0.993   0.982   0.982   0.982
111   T        -1.013  -1.013  -1.013  -1.006  -1.006  -1.006  -1.013  -1.013  -1.013
112   D        -1.013  -1.013  -1.013  -0.875  -0.875  -0.875  -1.013  -1.013  -1.013
113   G         0.982   0.982   0.982   0.689   0.689   0.689   0.982   0.982   0.982
114   T        -1.013  -1.013  -1.013  -0.851  -0.851  -0.851  -1.013  -1.013  -1.013
115   G         0.982   0.982   0.982   0.689   0.689   0.689   0.982   0.982   0.982
116   V        -1.013  -1.013  -1.013  -0.982  -0.982  -0.982  -1.013  -1.013  -1.013
117   G         0.982   0.982   0.982   0.689   0.689   0.689   0.982   0.982   0.982
118   G         0.982   0.982   0.982   0.689   0.689   0.689   0.982   0.982   0.982
119   K        -1.013  -1.013  -1.013  -0.934  -0.934  -0.934  -1.013  -1.013  -1.013
120   S         0.982   0.982   0.982   1.009   1.009   1.009   0.982   0.982   0.982
121   I        -1.013  -1.013  -1.013  -0.828  -0.828  -0.828  -1.013  -1.013  -1.013
122   Y        -1.013  -1.013  -1.013  -0.875  -0.875  -0.875  -1.013  -1.013  -1.013
123   G        -1.013  -1.013  -1.013  -1.018  -1.018  -1.018  -1.013  -1.013  -1.013
124   D        -1.013  -1.013  -1.013  -0.982  -0.982  -0.982  -1.013  -1.013  -1.013
125   T        -1.013  -1.013  -1.013  -0.982  -0.982  -0.982  -1.013  -1.013  -1.013
126   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
127   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
128   F         0.982   0.982   0.982   0.993   0.993   0.993   0.982   0.982   0.982
129   P        -1.013  -1.013  -1.013  -0.875  -0.875  -0.875  -1.013  -1.013  -1.013
130   D         0.982   0.982   0.982   0.943   0.943   0.943   0.982   0.982   0.982
131   E         0.982   0.982   0.982   1.042   1.042   1.042   0.982   0.982   0.982
132   N         0.982   0.982   0.982   1.075   1.075   1.075   0.982   0.982   0.982
133   F         0.982   0.982   0.982   0.993   0.993   0.993   0.982   0.982   0.982
134   T        -1.013  -1.013  -1.013  -1.006  -1.006  -1.006  -1.013  -1.013  -1.013
135   L         0.982   0.982   0.982   0.976   0.976   0.976   0.982   0.982   0.982
136   K         0.982   0.982   0.982   0.859   0.859   0.859   0.982   0.982   0.982
137   H         0.982   0.982   0.982   1.267   1.267   1.267   0.982   0.982   0.982
138   D         0.982   0.982   0.982   0.943   0.943   0.943   0.982   0.982   0.982
139   R         0.982   0.982   0.982   1.204   1.204   1.204   0.982   0.982   0.982
140   K         0.982   0.982   0.982   0.859   0.859   0.859   0.982   0.982   0.982
141   G        -1.013  -1.013  -1.013  -1.054  -1.054  -1.054  -1.013  -1.013  -1.013
142   R         0.982   0.982   0.982   1.204   1.204   1.204   0.982   0.982   0.982
143   L         0.982   0.982   0.982   0.976   0.976   0.976   0.982   0.982   0.982
144   S         0.982   0.982   0.982   1.009   1.009   1.009   0.982   0.982   0.982
145   M         0.982   0.982   0.982   1.299   1.299   1.299   0.982   0.982   0.982
146   A         0.982   0.982   0.982   1.172   1.172   1.172   0.982   0.982   0.982
147   N         0.982   0.982   0.982   1.075   1.075   1.075   0.982   0.982   0.982
148   R        -1.013  -1.013  -1.013  -0.725  -0.725  -0.725  -1.013  -1.013  -1.013
149   G         0.982   0.982   0.982   0.689   0.689   0.689   0.982   0.982   0.982
150   K         0.982   0.982   0.982   0.859   0.859   0.859   0.982   0.982   0.982
151   D        -1.013  -1.013  -1.013  -0.982  -0.982  -0.982  -1.013  -1.013  -1.013
152   T         0.982   0.982   0.982   0.943   0.943   0.943   0.982   0.982   0.982
153   N         0.982   0.982   0.982   1.075   1.075   1.075   0.982   0.982   0.982
154   G         0.982   0.982   0.982   0.689   0.689   0.689   0.982   0.982   0.982
155   S         0.982   0.982   0.982   1.009   1.009   1.009   0.982   0.982   0.982
156   Q         0.982   0.982   0.982   1.251   1.251   1.251   0.982   0.982   0.982
157   F         0.982   0.982   0.982   0.993   0.993   0.993   0.982   0.982   0.982
158   F         0.982   0.982   0.982   0.993   0.993   0.993   0.982   0.982   0.982
159   I         0.982   0.982   0.982   1.009   1.009   1.009   0.982   0.982   0.982
160   T         0.982   0.982   0.982   0.943   0.943   0.943   0.982   0.982   0.982
161   T         0.982   0.982   0.982   0.943   0.943   0.943   0.982   0.982   0.982
162   T        -1.013  -1.013  -1.013  -0.910  -0.910  -0.910  -1.013  -1.013  -1.013
163   E        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
164   E        -1.013  -1.013  -1.013  -0.982  -0.982  -0.982  -1.013  -1.013  -1.013
165   A        -1.013  -1.013  -1.013  -0.910  -0.910  -0.910  -1.013  -1.013  -1.013
166   S        -1.013  -1.013  -1.013  -1.091  -1.091  -1.091  -1.013  -1.013  -1.013
167   W         0.982   0.982   0.982   1.330   1.330   1.330   0.982   0.982   0.982
168   L         0.982   0.982   0.982   0.976   0.976   0.976   0.982   0.982   0.982
169   D         0.982   0.982   0.982   0.943   0.943   0.943   0.982   0.982   0.982
170   G         0.982   0.982   0.982   0.689   0.689   0.689   0.982   0.982   0.982
171   K        -1.013  -1.013  -1.013  -0.970  -0.970  -0.970  -1.013  -1.013  -1.013
172   H         0.982   0.982   0.982   1.267   1.267   1.267   0.982   0.982   0.982
173   V         0.982   0.982   0.982   0.792   0.792   0.792   0.982   0.982   0.982
174   V         0.982   0.982   0.982   0.792   0.792   0.792   0.982   0.982   0.982
175   F         0.982   0.982   0.982   0.993   0.993   0.993   0.982   0.982   0.982
176   G         0.982   0.982   0.982   0.689   0.689   0.689   0.982   0.982   0.982
177   Q        -1.013  -1.013  -1.013  -0.782  -0.782  -0.782  -1.013  -1.013  -1.013
178   V         0.982   0.982   0.982   0.792   0.792   0.792   0.982   0.982   0.982
179   V        -1.013  -1.013  -1.013  -1.165  -1.165  -1.165  -1.013  -1.013  -1.013
180   D         0.982   0.982   0.982   0.943   0.943   0.943   0.982   0.982   0.982
181   G         0.982   0.982   0.982   0.689   0.689   0.689   0.982   0.982   0.982
182   M        -1.013  -1.013  -1.013  -0.875  -0.875  -0.875  -1.013  -1.013  -1.013
183   D         0.982   0.982   0.982   0.943   0.943   0.943   0.982   0.982   0.982
184   V         0.982   0.982   0.982   0.792   0.792   0.792   0.982   0.982   0.982
185   V         0.982   0.982   0.982   0.792   0.792   0.792   0.982   0.982   0.982
186   N        -1.013  -1.013  -1.013  -0.982  -0.982  -0.982  -1.013  -1.013  -1.013
187   Y         0.982   0.982   0.982   1.219   1.219   1.219   0.982   0.982   0.982
188   I         0.982   0.982   0.982   1.009   1.009   1.009   0.982   0.982   0.982
189   Q        -1.013  -1.013  -1.013  -0.782  -0.782  -0.782  -1.013  -1.013  -1.013
190   H        -1.013  -1.013  -1.013  -0.748  -0.748  -0.748  -1.013  -1.013  -1.013
191   V         0.982   0.982   0.982   0.792   0.792   0.792   0.982   0.982   0.982
192   S        -1.013  -1.013  -1.013  -1.091  -1.091  -1.091  -1.013  -1.013  -1.013
193   R        -1.013  -1.013  -1.013  -0.887  -0.887  -0.887  -1.013  -1.013  -1.013
194   D        -1.013  -1.013  -1.013  -1.267  -1.267  -1.267  -1.013  -1.013  -1.013
195   A        -1.013  -1.013  -1.013  -0.725  -0.725  -0.725  -1.013  -1.013  -1.013
196   N        -1.013  -1.013  -1.013  -1.165  -1.165  -1.165  -1.013  -1.013  -1.013
197   D         0.982   0.982   0.982   0.943   0.943   0.943   0.982   0.982   0.982
198   K        -1.013  -1.013  -1.013  -0.946  -0.946  -0.946  -1.013  -1.013  -1.013
199   P         0.982   0.982   0.982   1.219   1.219   1.219   0.982   0.982   0.982
200   L        -1.013  -1.013  -1.013  -1.165  -1.165  -1.165  -1.013  -1.013  -1.013
201   E        -1.013  -1.013  -1.013  -1.066  -1.066  -1.066  -1.013  -1.013  -1.013
202   A        -1.013  -1.013  -1.013  -0.840  -0.840  -0.840  -1.013  -1.013  -1.013
203   V        -1.013  -1.013  -1.013  -1.140  -1.140  -1.140  -1.013  -1.013  -1.013
204   K         0.982   0.982   0.982   0.859   0.859   0.859   0.982   0.982   0.982
205   I         0.982   0.982   0.982   1.009   1.009   1.009   0.982   0.982   0.982
206   A        -1.013  -1.013  -1.013  -1.018  -1.018  -1.018  -1.013  -1.013  -1.013
207   K        -1.013  -1.013  -1.013  -0.970  -0.970  -0.970  -1.013  -1.013  -1.013
208   C        -1.013  -1.013  -1.013  -0.736  -0.736  -0.736  -1.013  -1.013  -1.013
209   G         0.982   0.982   0.982   0.689   0.689   0.689   0.982   0.982   0.982
210   E         0.982   0.982   0.982   1.042   1.042   1.042   0.982   0.982   0.982
211   W        -1.013  -1.013  -1.013  -0.771  -0.771  -0.771  -1.013  -1.013  -1.013
212   T        -1.013  -1.013  -1.013  -1.140  -1.140  -1.140  -1.013  -1.013  -1.013
213   P        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
214   E        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
215   L        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
216   S        -1.013  -1.013  -1.013  -1.030  -1.030  -1.030  -1.013  -1.013  -1.013
217   S        -1.013  -1.013  -1.013  -1.091  -1.091  -1.091  -1.013  -1.013  -1.013
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P23285
Output conservation file - output/P23285
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
