1     M         1.337   1.342   1.414   1.575   1.580   1.689   1.452   1.458   1.574
2     P        -0.056  -0.070  -0.275  -0.142  -0.165  -0.443  -0.088  -0.108  -0.412
3     S        -1.373  -1.346  -1.259  -1.422  -1.387  -1.266  -1.207  -1.183  -1.050
4     D        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
5     V        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
6     A        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
7     S        -1.719  -1.691  -1.591  -1.549  -1.510  -1.390  -1.347  -1.323  -1.182
8     R        -0.896  -0.843  -0.847  -1.190  -1.121  -1.101  -0.928  -0.875  -0.840
9     T         1.337   1.342   1.414   1.398   1.402   1.520   1.452   1.458   1.574
10    G         1.337   1.342   1.414   1.315   1.322   1.441   1.452   1.458   1.574
11    L         1.337   1.342   1.414   1.297   1.303   1.424   1.452   1.458   1.574
12    P         0.678   0.649   0.431   0.876   0.841   0.537   0.702   0.656   0.263
13    T        -1.996  -1.994  -1.840  -1.971  -1.978  -1.750  -1.441  -1.438  -1.255
14    P        -0.784  -0.824  -0.855  -0.594  -0.656  -0.717  -0.798  -0.843  -0.847
15    W         1.337   1.342   1.414   1.572   1.578   1.686   1.452   1.458   1.574
16    T        -1.276  -1.257  -1.242  -1.190  -1.181  -1.140  -1.120  -1.106  -1.036
17    V         0.180   0.193   0.263   0.118   0.139   0.221  -0.156  -0.135  -0.054
18    R         0.354   0.361   0.310   0.264   0.277   0.145   0.166   0.177   0.037
19    Y        -1.390  -1.376  -1.276  -1.079  -1.068  -0.926  -1.227  -1.213  -1.064
20    S         1.337   1.342   1.414   1.127   1.129   1.262   1.452   1.458   1.574
21    K        -0.237  -0.207  -0.314  -0.254  -0.193  -0.362  -0.370  -0.318  -0.471
22    S         0.354   0.356   0.305   0.111   0.108   0.032   0.166   0.167   0.028
23    K        -0.481  -0.511  -0.414  -0.625  -0.671  -0.528  -0.691  -0.735  -0.608
24    K         0.180   0.226   0.289   0.078   0.171   0.236  -0.156  -0.071  -0.003
25    R        -0.481  -0.502  -0.415  -0.676  -0.717  -0.608  -0.691  -0.722  -0.610
26    E         0.180   0.162   0.255  -0.081  -0.109   0.043  -0.156  -0.194  -0.070
27    Y         1.337   1.342   1.414   1.541   1.548   1.656   1.452   1.458   1.574
28    F         0.180   0.179   0.259   0.210   0.208   0.316  -0.156  -0.160  -0.062
29    F         0.354   0.343   0.321   0.416   0.397   0.311   0.166   0.145   0.059
30    N         0.051   0.073  -0.251   0.377   0.400  -0.060   0.059   0.087  -0.378
31    P        -0.784  -0.834  -0.850  -0.643  -0.722  -0.785  -0.798  -0.853  -0.841
32    E        -0.367  -0.403  -0.374  -0.626  -0.677  -0.633  -0.584  -0.623  -0.562
33    T         1.337   1.342   1.414   1.398   1.402   1.520   1.452   1.458   1.574
34    K        -0.540  -0.646  -0.792  -0.420  -0.562  -0.794  -0.477  -0.605  -0.772
35    H        -1.087  -1.060  -1.197  -0.960  -0.918  -1.102  -0.906  -0.877  -0.990
36    S         1.337   1.342   1.414   1.127   1.129   1.262   1.452   1.458   1.574
37    Q        -1.276  -1.255  -1.245  -1.303  -1.294  -1.235  -1.120  -1.094  -1.036
38    W         1.337   1.342   1.414   1.572   1.578   1.686   1.452   1.458   1.574
39    E         0.678   0.628   0.430   0.638   0.580   0.304   0.702   0.623   0.262
40    E        -0.237  -0.144  -0.320  -0.196  -0.050  -0.347  -0.370  -0.240  -0.479
41    P         1.337   1.342   1.414   1.433   1.439   1.553   1.452   1.458   1.574
42    E        -1.390  -1.387  -1.267  -1.492  -1.467  -1.260  -1.227  -1.220  -1.057
43    G         0.678   0.635   0.431   0.744   0.690   0.397   0.702   0.635   0.264
44    T         1.337   1.342   1.414   1.398   1.402   1.520   1.452   1.458   1.574
45    N         0.678   0.712   0.436   0.837   0.882   0.516   0.702   0.752   0.274
46    K        -1.996  -1.972  -1.836  -1.765  -1.731  -1.540  -1.441  -1.422  -1.253
47    D        -0.237  -0.274  -0.339  -0.237  -0.284  -0.435  -0.370  -0.416  -0.511
48    Q        -1.276  -1.340  -1.262  -1.127  -1.222  -1.137  -1.120  -1.174  -1.052
49    L         0.051   0.157  -0.241   0.143   0.279  -0.298   0.059   0.203  -0.363
50    H        -1.087  -1.040  -1.203  -0.861  -0.796  -1.000  -0.906  -0.857  -0.996
51    K        -1.087  -1.119  -1.199  -0.979  -1.016  -1.173  -0.906  -0.940  -0.992
52    H         0.051   0.038  -0.255   0.441   0.425   0.004   0.059   0.040  -0.384
53    L        -0.555  -0.555  -0.427  -0.644  -0.632  -0.429  -0.798  -0.796  -0.626
54    R        -0.540  -0.418  -0.778  -0.380  -0.208  -0.798  -0.477  -0.328  -0.756
55    D        -1.276  -1.264  -1.244  -1.233  -1.199  -1.178  -1.120  -1.103  -1.036
56    H        -1.390  -1.416  -1.268  -1.112  -1.151  -0.957  -1.227  -1.242  -1.058
57    -        -0.992  -1.007  -0.905  -0.957  -0.982  -0.823  -1.017  -1.033  -0.898
58    -        -0.992  -0.982  -0.895  -1.187  -1.170  -1.015  -1.017  -1.005  -0.888
59    -        -0.992  -1.007  -0.905  -0.945  -0.980  -0.849  -1.017  -1.033  -0.898
60    -        -0.992  -1.007  -0.905  -1.197  -1.233  -1.096  -1.017  -1.033  -0.898
61    -        -0.992  -1.010  -0.890  -0.932  -0.959  -0.807  -1.017  -1.036  -0.882
62    -        -0.508  -0.498  -0.412  -0.644  -0.625  -0.492  -0.743  -0.730  -0.608
63    -        -0.325  -0.319  -0.364  -0.238  -0.232  -0.341  -0.468  -0.461  -0.539
64    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
65    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
66    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
67    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
68    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
69    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
70    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
71    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
72    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
73    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
74    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
75    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
76    P        -1.276  -1.327  -1.268  -1.141  -1.230  -1.175  -1.120  -1.165  -1.058
77    V        -1.276  -1.273  -1.244  -1.165  -1.148  -1.084  -1.120  -1.109  -1.036
78    R        -0.367  -0.376  -0.361  -0.529  -0.517  -0.469  -0.584  -0.579  -0.541
79    V         0.180   0.162   0.255   0.118   0.077   0.203  -0.156  -0.194  -0.070
80    R         1.337   1.342   1.414   1.330   1.337   1.455   1.452   1.458   1.574
81    C        -0.555  -0.565  -0.430  -0.500  -0.519  -0.329  -0.798  -0.810  -0.630
82    L        -0.367  -0.349  -0.359  -0.684  -0.655  -0.630  -0.584  -0.548  -0.538
83    H         1.337   1.342   1.414   1.479   1.485   1.597   1.452   1.458   1.574
84    I         0.180   0.218   0.270  -0.029   0.036   0.103  -0.156  -0.087  -0.041
85    L         1.337   1.342   1.414   1.297   1.303   1.424   1.452   1.458   1.574
86    I         0.180   0.176   0.260   0.088   0.080   0.201  -0.156  -0.167  -0.059
87    K         1.337   1.342   1.414   1.261   1.274   1.389   1.452   1.458   1.574
88    H         0.051   0.032  -0.268   0.327   0.304  -0.180   0.059   0.031  -0.402
89    K        -0.555  -0.561  -0.428  -0.706  -0.706  -0.511  -0.798  -0.804  -0.628
90    D        -0.367  -0.400  -0.372  -0.391  -0.436  -0.405  -0.584  -0.622  -0.560
91    S         1.337   1.342   1.414   1.127   1.129   1.262   1.452   1.458   1.574
92    R         1.337   1.342   1.414   1.330   1.337   1.455   1.452   1.458   1.574
93    R         0.678   0.628   0.430   0.744   0.680   0.373   0.702   0.623   0.262
94    P         1.337   1.342   1.414   1.433   1.439   1.553   1.452   1.458   1.574
95    A        -0.555  -0.549  -0.422  -0.920  -0.927  -0.727  -0.798  -0.789  -0.619
96    S         1.337   1.342   1.414   1.127   1.129   1.262   1.452   1.458   1.574
97    H         0.180   0.226   0.289   0.261   0.344   0.414  -0.156  -0.071  -0.003
98    R         0.125   0.134   0.249  -0.190  -0.172   0.008  -0.263  -0.247  -0.081
99    S        -1.693  -1.645  -1.567  -1.651  -1.614  -1.422  -1.334  -1.297  -1.166
100   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
101   E        -0.784  -0.824  -0.855  -0.896  -0.953  -1.019  -0.798  -0.843  -0.847
102   N        -1.276  -1.281  -1.242  -1.125  -1.140  -1.111  -1.120  -1.125  -1.035
103   I         1.337   1.342   1.414   1.380   1.389   1.503   1.452   1.458   1.574
104   T         0.051  -0.026  -0.265   0.199   0.086  -0.346   0.059  -0.047  -0.397
105   I         0.180   0.226   0.289  -0.001   0.083   0.165  -0.156  -0.071  -0.003
106   S         0.354   0.347   0.298   0.288   0.270   0.112   0.166   0.152   0.014
107   K         0.354   0.343   0.321   0.219   0.206   0.141   0.166   0.145   0.059
108   Q        -0.555  -0.547  -0.428  -0.749  -0.736  -0.551  -0.798  -0.788  -0.628
109   D         0.678   0.712   0.436   0.638   0.684   0.314   0.702   0.752   0.274
110   A         0.678   0.628   0.430   0.758   0.688   0.358   0.702   0.623   0.262
111   T        -1.276  -1.246  -1.238  -1.207  -1.174  -1.174  -1.120  -1.098  -1.032
112   D        -1.996  -1.984  -1.837  -1.859  -1.824  -1.640  -1.441  -1.430  -1.254
113   E        -0.973  -0.994  -0.895  -1.142  -1.165  -0.992  -1.013  -1.035  -0.891
114   L         0.678   0.635   0.431   0.726   0.671   0.380   0.702   0.635   0.264
115   K        -0.367  -0.378  -0.367  -0.607  -0.617  -0.565  -0.584  -0.597  -0.554
116   T        -1.693  -1.688  -1.580  -1.616  -1.605  -1.477  -1.334  -1.327  -1.175
117   L        -1.276  -1.249  -1.231  -0.945  -0.916  -0.908  -1.120  -1.101  -1.027
118   I        -0.973  -0.990  -0.873  -1.012  -1.046  -0.878  -1.013  -1.025  -0.867
119   T        -1.693  -1.661  -1.568  -1.533  -1.489  -1.384  -1.334  -1.309  -1.166
120   R        -0.237  -0.274  -0.338  -0.287  -0.337  -0.474  -0.370  -0.415  -0.509
121   L         0.125   0.133   0.249  -0.126  -0.110   0.074  -0.263  -0.251  -0.080
122   D        -1.276  -1.194  -1.242  -1.289  -1.178  -1.228  -1.120  -1.047  -1.035
123   D        -1.276  -1.219  -1.228  -1.435  -1.353  -1.351  -1.120  -1.061  -1.024
124   D         0.354   0.406   0.345   0.292   0.374   0.259   0.166   0.257   0.104
125   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
126   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
127   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
128   S        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
129   K        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
130   T        -0.896  -0.882  -0.866  -1.166  -1.153  -1.116  -0.928  -0.913  -0.860
131   N        -1.693  -1.643  -1.566  -1.419  -1.349  -1.248  -1.334  -1.296  -1.165
132   S        -0.540  -0.562  -0.775  -0.557  -0.598  -0.886  -0.477  -0.506  -0.753
133   F        -0.858  -0.894  -0.864  -0.762  -0.806  -0.734  -0.906  -0.933  -0.858
134   E        -1.276  -1.316  -1.264  -1.329  -1.395  -1.284  -1.120  -1.157  -1.054
135   A        -0.973  -0.929  -0.875  -1.124  -1.059  -1.013  -1.013  -0.971  -0.870
136   L         0.678   0.736   0.439   0.729   0.797   0.399   0.702   0.787   0.279
137   A         1.337   1.342   1.414   1.362   1.367   1.486   1.452   1.458   1.574
138   K        -1.579  -1.638  -1.580  -1.388  -1.456  -1.380  -1.227  -1.278  -1.174
139   E        -0.858  -0.894  -0.864  -1.023  -1.062  -1.040  -0.906  -0.933  -0.858
140   R         0.180   0.226   0.289  -0.138  -0.053   0.035  -0.156  -0.071  -0.003
141   S         1.337   1.342   1.414   1.127   1.129   1.262   1.452   1.458   1.574
142   D         1.337   1.342   1.414   1.391   1.402   1.513   1.452   1.458   1.574
143   C         1.337   1.342   1.414   1.609   1.616   1.721   1.452   1.458   1.574
144   S         1.337   1.342   1.414   1.127   1.129   1.262   1.452   1.458   1.574
145   S         1.337   1.342   1.414   1.127   1.129   1.262   1.452   1.458   1.574
146   Y        -0.784  -0.729  -0.836  -0.593  -0.532  -0.634  -0.798  -0.743  -0.826
147   K        -0.858  -0.860  -0.863  -0.944  -0.930  -0.910  -0.906  -0.902  -0.857
148   R        -0.237  -0.231  -0.339  -0.254  -0.259  -0.414  -0.370  -0.373  -0.509
149   G         0.354   0.330   0.305   0.272   0.229   0.157   0.166   0.121   0.027
150   G         1.337   1.342   1.414   1.315   1.322   1.441   1.452   1.458   1.574
151   D         0.678   0.635   0.431   0.797   0.746   0.413   0.702   0.635   0.264
152   L         1.337   1.342   1.414   1.297   1.303   1.424   1.452   1.458   1.574
153   G         1.337   1.342   1.414   1.315   1.322   1.441   1.452   1.458   1.574
154   W        -0.784  -0.780  -0.832  -0.518  -0.515  -0.597  -0.798  -0.789  -0.824
155   F         1.337   1.342   1.414   1.475   1.484   1.593   1.452   1.458   1.574
156   G         0.678   0.628   0.430   0.711   0.641   0.314   0.702   0.623   0.262
157   R        -0.367  -0.329  -0.365  -0.511  -0.473  -0.469  -0.584  -0.550  -0.551
158   G         0.678   0.635   0.431   0.748   0.696   0.406   0.702   0.635   0.264
159   E        -0.237  -0.260  -0.339  -0.308  -0.337  -0.436  -0.370  -0.402  -0.507
160   M         1.337   1.342   1.414   1.575   1.580   1.689   1.452   1.458   1.574
161   Q         1.337   1.342   1.414   1.465   1.469   1.583   1.452   1.458   1.574
162   P        -0.237  -0.289  -0.353  -0.176  -0.263  -0.394  -0.370  -0.445  -0.528
163   S        -0.481  -0.524  -0.422  -0.819  -0.889  -0.715  -0.691  -0.753  -0.620
164   F         1.337   1.342   1.414   1.475   1.484   1.593   1.452   1.458   1.574
165   E         1.337   1.342   1.414   1.206   1.216   1.337   1.452   1.458   1.574
166   D        -0.973  -0.966  -0.876  -1.088  -1.057  -0.925  -1.013  -0.999  -0.869
167   A         1.337   1.342   1.414   1.362   1.367   1.486   1.452   1.458   1.574
168   A         0.678   0.649   0.431   0.758   0.717   0.359   0.702   0.656   0.263
169   F         1.337   1.342   1.414   1.475   1.484   1.593   1.452   1.458   1.574
170   Q        -0.540  -0.532  -0.786  -0.287  -0.279  -0.641  -0.477  -0.468  -0.765
171   L         1.337   1.342   1.414   1.297   1.303   1.424   1.452   1.458   1.574
172   K        -0.540  -0.584  -0.798  -0.405  -0.462  -0.774  -0.477  -0.531  -0.779
173   V        -0.973  -0.973  -0.881  -0.852  -0.846  -0.711  -1.013  -1.008  -0.876
174   G         0.354   0.423   0.346   0.292   0.399   0.260   0.166   0.285   0.105
175   E         0.354   0.370   0.305   0.210   0.237   0.134   0.166   0.192   0.028
176   V         0.180   0.176   0.260   0.118   0.106   0.216  -0.156  -0.167  -0.059
177   S         1.337   1.342   1.414   1.127   1.129   1.262   1.452   1.458   1.574
178   D        -0.237  -0.306  -0.341  -0.172  -0.273  -0.333  -0.370  -0.465  -0.512
179   I         0.678   0.635   0.431   0.823   0.774   0.490   0.702   0.635   0.264
180   V         0.354   0.343   0.321   0.432   0.413   0.339   0.166   0.145   0.059
181   E         0.678   0.665   0.436   0.638   0.627   0.313   0.702   0.681   0.272
182   S         0.125   0.138   0.251  -0.236  -0.197  -0.019  -0.263  -0.239  -0.078
183   G        -1.276  -1.317  -1.244  -1.185  -1.255  -1.166  -1.120  -1.157  -1.037
184   S         1.337   1.342   1.414   1.127   1.129   1.262   1.452   1.458   1.574
185   G         1.337   1.342   1.414   1.315   1.322   1.441   1.452   1.458   1.574
186   V        -0.858  -0.862  -0.849  -0.794  -0.797  -0.752  -0.906  -0.907  -0.844
187   H         1.337   1.342   1.414   1.479   1.485   1.597   1.452   1.458   1.574
188   V        -0.367  -0.347  -0.354  -0.467  -0.449  -0.411  -0.584  -0.567  -0.539
189   I         0.678   0.628   0.430   0.799   0.737   0.431   0.702   0.623   0.262
190   K        -0.555  -0.555  -0.427  -0.779  -0.767  -0.590  -0.798  -0.796  -0.626
191   R         1.337   1.342   1.414   1.330   1.337   1.455   1.452   1.458   1.574
192   V        -0.858  -0.885  -0.865  -0.743  -0.776  -0.741  -0.906  -0.925  -0.859
193   G        -0.367  -0.369  -0.355  -0.517  -0.518  -0.497  -0.584  -0.582  -0.540
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P22696
Output conservation file - output/P22696
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
