1     M         1.244   1.251   1.312   1.447   1.455   1.539   1.323   1.332   1.425
2     I        -0.594  -0.624  -0.565  -0.738  -0.772  -0.714  -0.774  -0.791  -0.698
3     K        -1.044  -1.025  -1.051  -1.119  -1.111  -1.147  -0.994  -0.979  -0.965
4     S         1.244   1.251   1.312   1.070   1.085   1.196   1.323   1.332   1.425
5     T         1.244   1.251   1.312   1.382   1.377   1.479   1.323   1.332   1.425
6     L        -0.258  -0.344  -0.509  -0.335  -0.459  -0.677  -0.262  -0.384  -0.617
7     I        -0.143  -0.202  -0.464  -0.019  -0.105  -0.543  -0.112  -0.193  -0.556
8     Y        -1.044  -1.135  -1.071  -0.901  -1.034  -0.941  -0.994  -1.087  -0.986
9     R         1.244   1.251   1.312   1.299   1.299   1.404   1.323   1.332   1.425
10    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
11    E        -1.679  -1.715  -1.510  -1.752  -1.861  -1.580  -1.415  -1.433  -1.196
12    D         1.244   1.251   1.312   1.199   1.210   1.313   1.323   1.332   1.425
13    G         0.116   0.205   0.176   0.186   0.330   0.241  -0.118   0.031  -0.065
14    L         1.244   1.251   1.312   1.016   1.020   1.146   1.323   1.332   1.425
15    P         0.534   0.452   0.252   0.842   0.742   0.438   0.550   0.427   0.072
16    L         1.244   1.251   1.312   1.016   1.020   1.146   1.323   1.332   1.425
17    C        -0.143  -0.257  -0.479   0.266   0.104  -0.325  -0.112  -0.263  -0.576
18    T        -1.044  -0.909  -1.029  -0.913  -0.721  -0.918  -0.994  -0.830  -0.938
19    S         1.244   1.251   1.312   1.085   1.102   1.209   1.323   1.332   1.425
20    V         1.244   1.251   1.312   1.333   1.345   1.435   1.323   1.332   1.425
21    D         1.244   1.251   1.312   1.211   1.224   1.324   1.323   1.332   1.425
22    N         0.534   0.593   0.268   0.595   0.673   0.227   0.550   0.633   0.099
23    E        -0.559  -0.435  -0.532  -0.567  -0.384  -0.538  -0.695  -0.538  -0.650
24    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
25    N        -1.124  -1.105  -1.061  -1.037  -0.994  -0.946  -1.105  -1.073  -0.976
26    D        -1.044  -1.060  -1.071  -0.995  -1.036  -1.013  -0.994  -1.029  -0.985
27    P        -1.371  -1.494  -1.465  -1.074  -1.294  -1.366  -1.105  -1.225  -1.157
28    S        -0.004  -0.008   0.102  -0.329  -0.325  -0.146  -0.332  -0.346  -0.200
29    L         1.244   1.251   1.312   1.016   1.020   1.146   1.323   1.332   1.425
30    F        -2.352  -2.246  -2.094  -2.096  -1.980  -1.720  -1.656  -1.574  -1.380
31    E        -0.454  -0.436  -0.510  -0.448  -0.404  -0.586  -0.553  -0.508  -0.619
32    Q        -0.143  -0.299  -0.485   0.162  -0.034  -0.320  -0.112  -0.318  -0.585
33    K         1.244   1.251   1.312   1.179   1.197   1.295   1.323   1.332   1.425
34    Q        -0.143  -0.169  -0.465  -0.081  -0.119  -0.577  -0.112  -0.151  -0.558
35    K        -0.594  -0.581  -0.567  -0.730  -0.697  -0.646  -0.774  -0.766  -0.703
36    V        -1.124  -1.143  -1.073  -0.923  -0.950  -0.871  -1.105  -1.112  -0.989
37    K         1.244   1.251   1.312   1.179   1.197   1.295   1.323   1.332   1.425
38    I        -0.594  -0.581  -0.567  -0.845  -0.867  -0.758  -0.774  -0.766  -0.703
39    V        -0.797  -0.800  -0.617  -1.140  -1.123  -0.840  -0.994  -0.994  -0.760
40    V        -1.248  -1.259  -1.104  -1.407  -1.457  -1.189  -1.215  -1.220  -1.017
41    S        -0.781  -0.948  -1.042  -0.758  -0.993  -1.139  -0.663  -0.856  -0.951
42    R         0.534   0.499   0.257   0.660   0.607   0.229   0.550   0.498   0.080
43    L        -1.044  -0.915  -1.026  -1.196  -1.007  -1.108  -0.994  -0.837  -0.935
44    T         0.183   0.121   0.140   0.188   0.055   0.022  -0.001  -0.114  -0.130
45    P        -0.143  -0.254  -0.482   0.210   0.056  -0.330  -0.112  -0.259  -0.580
46    Q        -0.797  -0.755  -0.596  -0.844  -0.775  -0.548  -0.994  -0.940  -0.734
47    S         1.244   1.251   1.312   1.085   1.102   1.209   1.323   1.332   1.425
48    A        -0.063  -0.046   0.092  -0.250  -0.233  -0.005  -0.443  -0.417  -0.217
49    T        -1.698  -1.712  -1.494  -1.536  -1.558  -1.273  -1.436  -1.432  -1.184
50    E        -1.248  -1.209  -1.107  -1.247  -1.212  -1.060  -1.215  -1.183  -1.021
51    A         1.244   1.251   1.312   1.312   1.313   1.416   1.323   1.332   1.425
52    T        -0.063  -0.037   0.097  -0.374  -0.351  -0.123  -0.443  -0.401  -0.210
53    L        -0.454  -0.372  -0.523  -0.546  -0.410  -0.668  -0.553  -0.444  -0.637
54    E         1.244   1.251   1.312   1.330   1.333   1.433   1.323   1.332   1.425
55    S         1.244   1.251   1.312   1.085   1.102   1.209   1.323   1.332   1.425
56    G         0.534   0.560   0.265   0.784   0.817   0.352   0.550   0.587   0.094
57    S        -1.044  -1.006  -1.048  -1.107  -1.063  -1.112  -0.994  -0.960  -0.963
58    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
59    F        -0.454  -0.380  -0.527  -0.430  -0.308  -0.593  -0.553  -0.449  -0.644
60    E        -1.124  -1.067  -1.069  -1.007  -0.940  -0.908  -1.105  -1.046  -0.984
61    I        -0.143  -0.299  -0.485  -0.025  -0.244  -0.595  -0.112  -0.318  -0.585
62    H         1.244   1.251   1.312   1.520   1.527   1.605   1.323   1.332   1.425
63    Y         1.244   1.251   1.312   1.315   1.326   1.418   1.323   1.332   1.425
64    L         0.183   0.229   0.171  -0.117  -0.049  -0.137  -0.001   0.071  -0.073
65    K        -1.044  -1.000  -1.057  -0.984  -0.906  -1.002  -0.994  -0.939  -0.970
66    K        -1.248  -1.216  -1.101  -1.444  -1.387  -1.239  -1.215  -1.184  -1.016
67    S        -1.044  -1.061  -1.059  -1.017  -1.046  -1.088  -0.994  -1.012  -0.974
68    M        -1.574  -1.507  -1.459  -1.417  -1.292  -1.236  -1.325  -1.250  -1.153
69    V        -0.004   0.041   0.126  -0.183  -0.098   0.016  -0.332  -0.257  -0.155
70    Y        -1.124  -1.118  -1.060  -1.052  -1.012  -0.907  -1.105  -1.089  -0.976
71    Y         1.244   1.251   1.312   1.315   1.326   1.418   1.323   1.332   1.425
72    F        -1.248  -1.227  -1.096  -1.433  -1.390  -1.209  -1.215  -1.183  -1.011
73    V        -1.124  -1.095  -1.077  -0.960  -0.905  -0.918  -1.105  -1.072  -0.991
74    I         0.534   0.585   0.268   0.638   0.710   0.263   0.550   0.621   0.099
75    C        -0.781  -0.842  -1.017  -0.326  -0.423  -0.778  -0.663  -0.736  -0.924
76    E        -0.454  -0.383  -0.535  -0.401  -0.315  -0.556  -0.553  -0.472  -0.659
77    S        -1.698  -1.688  -1.490  -1.816  -1.781  -1.479  -1.436  -1.415  -1.180
78    G        -0.454  -0.372  -0.523  -0.551  -0.430  -0.615  -0.553  -0.444  -0.637
79    Y         1.244   1.251   1.312   1.315   1.326   1.418   1.323   1.332   1.425
80    P         1.244   1.251   1.312   1.444   1.454   1.536   1.323   1.332   1.425
81    R         0.534   0.615   0.271   0.660   0.753   0.256   0.550   0.664   0.105
82    N        -0.004   0.041   0.126  -0.246  -0.159  -0.040  -0.332  -0.257  -0.155
83    L         1.244   1.251   1.312   1.016   1.020   1.146   1.323   1.332   1.425
84    A         1.244   1.251   1.312   1.312   1.313   1.416   1.323   1.332   1.425
85    F         1.244   1.251   1.312   1.299   1.310   1.404   1.323   1.332   1.425
86    S         0.534   0.452   0.252   0.475   0.380   0.109   0.550   0.427   0.072
87    Y         1.244   1.251   1.312   1.315   1.326   1.418   1.323   1.332   1.425
88    L         1.244   1.251   1.312   1.016   1.020   1.146   1.323   1.332   1.425
89    N        -1.574  -1.589  -1.483  -1.612  -1.623  -1.454  -1.325  -1.328  -1.174
90    D        -0.063  -0.047   0.092  -0.323  -0.299  -0.072  -0.443  -0.419  -0.218
91    I        -0.454  -0.486  -0.541  -0.546  -0.631  -0.718  -0.553  -0.612  -0.665
92    A        -1.044  -0.961  -1.036  -1.043  -0.892  -1.037  -0.994  -0.887  -0.945
93    Q        -1.698  -1.661  -1.502  -1.396  -1.349  -1.149  -1.436  -1.392  -1.189
94    E         1.244   1.251   1.312   1.330   1.333   1.433   1.323   1.332   1.425
95    F         1.244   1.251   1.312   1.299   1.310   1.404   1.323   1.332   1.425
96    E        -1.698  -1.672  -1.492  -1.440  -1.408  -1.166  -1.436  -1.406  -1.182
97    H        -1.124  -1.175  -1.089  -0.930  -0.991  -0.902  -1.105  -1.140  -1.003
98    S         0.534   0.452   0.252   0.475   0.380   0.109   0.550   0.427   0.072
99    F        -0.717  -0.696  -0.599  -0.612  -0.584  -0.501  -0.884  -0.857  -0.738
100   A        -0.781  -0.785  -1.012  -0.434  -0.448  -0.851  -0.663  -0.672  -0.918
101   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
102   N        -1.574  -1.617  -1.477  -1.337  -1.396  -1.202  -1.325  -1.354  -1.169
103   E         0.183   0.164   0.131   0.221   0.183   0.095  -0.001  -0.039  -0.146
104   Y        -0.594  -0.546  -0.562  -0.826  -0.783  -0.759  -0.774  -0.724  -0.697
105   P        -1.044  -1.033  -1.066  -1.242  -1.215  -1.184  -0.994  -0.976  -0.978
106   K        -1.044  -1.093  -1.059  -1.137  -1.154  -1.127  -0.994  -1.027  -0.969
107   P        -1.044  -0.961  -1.036  -0.843  -0.695  -0.885  -0.994  -0.887  -0.945
108   T        -1.574  -1.457  -1.472  -1.386  -1.224  -1.295  -1.325  -1.229  -1.166
109   V        -1.248  -1.207  -1.090  -1.391  -1.373  -1.174  -1.215  -1.172  -1.004
110   R         1.244   1.251   1.312   1.299   1.299   1.404   1.323   1.332   1.425
111   P         1.244   1.251   1.312   1.444   1.454   1.536   1.323   1.332   1.425
112   Y         0.534   0.638   0.272   0.693   0.821   0.310   0.550   0.695   0.106
113   Q        -1.574  -1.478  -1.455  -1.376  -1.248  -1.227  -1.325  -1.223  -1.149
114   F         1.244   1.251   1.312   1.299   1.310   1.404   1.323   1.332   1.425
115   V        -1.371  -1.284  -1.429  -1.075  -0.944  -1.213  -1.105  -1.020  -1.125
116   N        -1.371  -1.472  -1.460  -1.303  -1.445  -1.415  -1.105  -1.205  -1.153
117   F         1.244   1.251   1.312   1.299   1.310   1.404   1.323   1.332   1.425
118   D         1.244   1.251   1.312   1.211   1.224   1.324   1.323   1.332   1.425
119   N        -0.594  -0.648  -0.562  -0.624  -0.703  -0.591  -0.774  -0.830  -0.696
120   F         1.244   1.251   1.312   1.299   1.310   1.404   1.323   1.332   1.425
121   L        -0.454  -0.570  -0.560  -0.623  -0.785  -0.727  -0.553  -0.708  -0.689
122   Q        -0.143  -0.129  -0.461   0.106   0.111  -0.399  -0.112  -0.102  -0.552
123   M        -0.454  -0.434  -0.542  -0.490  -0.478  -0.597  -0.553  -0.543  -0.668
124   T         0.534   0.449   0.256   0.731   0.605   0.270   0.550   0.422   0.079
125   K         1.244   1.251   1.312   1.179   1.197   1.295   1.323   1.332   1.425
126   K        -0.454  -0.570  -0.560  -0.516  -0.693  -0.670  -0.553  -0.708  -0.689
127   S        -1.574  -1.522  -1.462  -1.471  -1.365  -1.326  -1.325  -1.264  -1.155
128   Y         1.244   1.251   1.312   1.315   1.326   1.418   1.323   1.332   1.425
129   S        -0.797  -0.786  -0.619  -1.038  -1.014  -0.760  -0.994  -0.977  -0.762
130   D         0.534   0.449   0.256   0.601   0.498   0.224   0.550   0.422   0.079
131   K        -1.248  -1.260  -1.106  -1.251  -1.245  -0.999  -1.215  -1.216  -1.020
132   K         0.183   0.233   0.184   0.135   0.210   0.091  -0.001   0.079  -0.049
133   V        -0.004   0.041   0.126  -0.133  -0.061   0.060  -0.332  -0.257  -0.155
134   Q        -0.454  -0.571  -0.561  -0.314  -0.490  -0.494  -0.553  -0.709  -0.691
135   D        -1.574  -1.563  -1.473  -1.521  -1.462  -1.303  -1.325  -1.300  -1.165
136   N         1.244   1.251   1.312   1.340   1.360   1.441   1.323   1.332   1.425
137   L         0.534   0.585   0.268   0.416   0.472   0.097   0.550   0.621   0.099
138   D         1.244   1.251   1.312   1.211   1.224   1.324   1.323   1.332   1.425
139   Q        -0.004   0.041   0.126  -0.238  -0.155  -0.031  -0.332  -0.257  -0.155
140   L         1.244   1.251   1.312   1.016   1.020   1.146   1.323   1.332   1.425
141   N         0.534   0.452   0.252   0.749   0.662   0.375   0.550   0.427   0.072
142   Q        -1.248  -1.259  -1.109  -1.187  -1.198  -1.003  -1.215  -1.215  -1.022
143   E        -0.004   0.073   0.126  -0.181  -0.036   0.011  -0.332  -0.198  -0.155
144   L         1.244   1.251   1.312   1.016   1.020   1.146   1.323   1.332   1.425
145   V        -2.025  -1.977  -1.815  -1.890  -1.828  -1.599  -1.546  -1.503  -1.295
146   G        -0.004   0.041   0.126  -0.173  -0.099   0.030  -0.332  -0.257  -0.155
147   V         1.244   1.251   1.312   1.333   1.345   1.435   1.323   1.332   1.425
148   K        -0.004  -0.035   0.094  -0.223  -0.273  -0.059  -0.332  -0.397  -0.214
149   Q        -0.797  -0.799  -0.617  -0.994  -0.996  -0.725  -0.994  -0.993  -0.760
150   I        -0.004   0.041   0.126  -0.155  -0.067   0.036  -0.332  -0.257  -0.155
151   M         1.244   1.251   1.312   1.447   1.455   1.539   1.323   1.332   1.425
152   S         0.183   0.233   0.184   0.188   0.264   0.123  -0.001   0.079  -0.049
153   K         1.244   1.251   1.312   1.179   1.197   1.295   1.323   1.332   1.425
154   N         1.244   1.251   1.312   1.340   1.360   1.441   1.323   1.332   1.425
155   I         1.244   1.251   1.312   1.255   1.273   1.364   1.323   1.332   1.425
156   E         1.244   1.251   1.312   1.330   1.333   1.433   1.323   1.332   1.425
157   D         1.244   1.251   1.312   1.211   1.224   1.324   1.323   1.332   1.425
158   L         1.244   1.251   1.312   1.016   1.020   1.146   1.323   1.332   1.425
159   L         1.244   1.251   1.312   1.016   1.020   1.146   1.323   1.332   1.425
160   Y         0.534   0.499   0.257   0.725   0.687   0.361   0.550   0.498   0.080
161   R         1.244   1.251   1.312   1.299   1.299   1.404   1.323   1.332   1.425
162   G         1.244   1.251   1.312   1.423   1.430   1.517   1.323   1.332   1.425
163   D         0.534   0.499   0.257   0.622   0.587   0.273   0.550   0.498   0.080
164   S         1.244   1.251   1.312   1.085   1.102   1.209   1.323   1.332   1.425
165   L         1.244   1.251   1.312   1.016   1.020   1.146   1.323   1.332   1.425
166   D        -0.004  -0.027   0.094  -0.223  -0.269  -0.072  -0.332  -0.381  -0.215
167   K        -0.143  -0.254  -0.482  -0.017  -0.154  -0.464  -0.112  -0.259  -0.580
168   M         1.244   1.251   1.312   1.447   1.455   1.539   1.323   1.332   1.425
169   S        -0.781  -0.901  -1.024  -0.735  -0.893  -1.069  -0.663  -0.804  -0.932
170   D         0.534   0.452   0.252   0.593   0.493   0.198   0.550   0.427   0.072
171   M        -0.004   0.041   0.126  -0.329  -0.269  -0.106  -0.332  -0.257  -0.155
172   S         1.244   1.251   1.312   1.085   1.102   1.209   1.323   1.332   1.425
173   S        -0.143  -0.077  -0.453  -0.140  -0.048  -0.560  -0.112  -0.027  -0.540
174   S        -0.781  -0.948  -1.042  -0.755  -0.987  -1.133  -0.663  -0.856  -0.951
175   L         1.244   1.251   1.312   1.016   1.020   1.146   1.323   1.332   1.425
176   K        -0.004   0.050   0.115  -0.226  -0.127  -0.054  -0.332  -0.240  -0.175
177   E        -2.352  -2.335  -2.095  -2.019  -2.011  -1.697  -1.656  -1.636  -1.381
178   T        -1.044  -1.048  -1.056  -1.103  -1.130  -1.120  -0.994  -1.004  -0.971
179   S         0.534   0.585   0.268   0.465   0.534   0.108   0.550   0.621   0.099
180   K        -0.781  -0.706  -1.000  -0.715  -0.605  -1.094  -0.663  -0.583  -0.906
181   R         0.534   0.593   0.268   0.557   0.637   0.185   0.550   0.633   0.099
182   Y         1.244   1.251   1.312   1.315   1.326   1.418   1.323   1.332   1.425
183   R        -0.004  -0.018   0.098  -0.226  -0.272  -0.097  -0.332  -0.365  -0.208
184   K        -0.594  -0.556  -0.552  -0.762  -0.719  -0.660  -0.774  -0.737  -0.686
185   S        -1.124  -0.994  -1.074  -1.194  -1.024  -1.034  -1.105  -0.979  -0.989
186   A         1.244   1.251   1.312   1.312   1.313   1.416   1.323   1.332   1.425
187   Q        -0.143  -0.299  -0.485   0.126  -0.083  -0.399  -0.112  -0.318  -0.585
188   K        -0.004  -0.008   0.102  -0.268  -0.279  -0.103  -0.332  -0.346  -0.200
189   I         0.534   0.449   0.256   0.638   0.539   0.244   0.550   0.422   0.079
190   N         1.244   1.251   1.312   1.340   1.360   1.441   1.323   1.332   1.425
191   F        -0.454  -0.571  -0.561  -0.364  -0.545  -0.560  -0.553  -0.709  -0.691
192   D        -0.004  -0.008   0.102  -0.223  -0.236  -0.061  -0.332  -0.346  -0.200
193   L        -0.063  -0.047   0.092  -0.478  -0.463  -0.214  -0.443  -0.419  -0.218
194   L         0.183   0.208   0.151  -0.050  -0.019  -0.073  -0.001   0.037  -0.109
195   I        -0.594  -0.633  -0.564  -0.824  -0.848  -0.703  -0.774  -0.805  -0.697
196   S        -0.594  -0.623  -0.568  -0.823  -0.848  -0.766  -0.774  -0.790  -0.700
197   Q         1.244   1.251   1.312   1.355   1.369   1.455   1.323   1.332   1.425
198   Y         1.244   1.251   1.312   1.315   1.326   1.418   1.323   1.332   1.425
199   A        -0.717  -0.746  -0.611  -0.760  -0.811  -0.621  -0.884  -0.925  -0.752
200   P         1.244   1.251   1.312   1.444   1.454   1.536   1.323   1.332   1.425
201   I        -0.454  -0.571  -0.561  -0.440  -0.612  -0.610  -0.553  -0.709  -0.691
202   V        -1.044  -1.136  -1.072  -0.972  -1.127  -1.073  -0.994  -1.088  -0.987
203   I        -1.124  -1.141  -1.071  -1.011  -1.033  -0.911  -1.105  -1.111  -0.986
204   V        -1.044  -0.926  -1.043  -0.884  -0.748  -0.880  -0.994  -0.881  -0.957
205   A        -0.063  -0.047   0.092  -0.183  -0.165   0.056  -0.443  -0.419  -0.218
206   F        -0.594  -0.623  -0.568  -0.947  -0.988  -0.888  -0.774  -0.790  -0.700
207   F        -0.594  -0.648  -0.563  -0.750  -0.835  -0.728  -0.774  -0.828  -0.698
208   F        -0.143  -0.254  -0.482   0.078  -0.068  -0.421  -0.112  -0.259  -0.580
209   V        -0.454  -0.570  -0.560  -0.485  -0.698  -0.726  -0.553  -0.708  -0.689
210   F         0.534   0.449   0.256   0.671   0.563   0.255   0.550   0.422   0.079
211   L        -0.717  -0.746  -0.611  -0.982  -1.027  -0.774  -0.884  -0.925  -0.752
212   F        -0.797  -0.759  -0.596  -1.119  -1.077  -0.815  -0.994  -0.947  -0.735
213   W         0.183   0.177   0.149   0.410   0.392   0.275  -0.001  -0.016  -0.113
214   W         0.183   0.294   0.201   0.410   0.581   0.380  -0.001   0.178  -0.019
215   I        -1.248  -1.207  -1.094  -1.152  -1.116  -0.954  -1.215  -1.177  -1.009
216   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
217   F         1.244   1.251   1.312   1.286   1.297   1.393   1.323   1.332   1.425
218   L        -0.454  -0.460  -0.541  -0.675  -0.719  -0.769  -0.553  -0.594  -0.666
219   K        -1.044  -1.095  -1.070  -0.976  -1.077  -1.045  -0.994  -1.058  -0.984
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P22214
Output conservation file - output/P22214
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
