1     M         0.551   0.564   0.589   0.919   0.903   0.889   0.572   0.587   0.618
2     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
3     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
4     Q        -1.331  -1.642  -1.741  -1.009  -1.501  -1.725  -1.299  -1.698  -1.773
5     T         0.551   0.564   0.589   0.508   0.533   0.575   0.572   0.587   0.618
6     L        -1.141  -0.831  -0.865  -1.523  -0.903  -1.068  -1.587  -1.069  -1.145
7     K         0.551   0.564   0.589   0.503   0.515   0.572   0.572   0.587   0.618
8     C         0.551   0.564   0.589   0.755   0.759   0.764   0.572   0.587   0.618
9     V         0.551   0.564   0.589   0.186   0.247   0.330   0.572   0.587   0.618
10    V         0.551   0.564   0.589   0.186   0.247   0.330   0.572   0.587   0.618
11    V        -0.413  -0.149  -0.846  -0.670  -0.313  -1.189  -0.436  -0.089  -1.113
12    G         0.551   0.564   0.589   0.620   0.628   0.661   0.572   0.587   0.618
13    D         0.551   0.564   0.589   0.538   0.549   0.598   0.572   0.587   0.618
14    G         0.551   0.564   0.589   0.620   0.628   0.661   0.572   0.587   0.618
15    A         0.551   0.564   0.589   0.596   0.602   0.643   0.572   0.587   0.618
16    V         0.551   0.564   0.589   0.186   0.247   0.330   0.572   0.587   0.618
17    G         0.551   0.564   0.589   0.620   0.628   0.661   0.572   0.587   0.618
18    K         0.551   0.564   0.589   0.503   0.515   0.572   0.572   0.587   0.618
19    T         0.551   0.564   0.589   0.508   0.533   0.575   0.572   0.587   0.618
20    C         0.551   0.564   0.589   0.755   0.759   0.764   0.572   0.587   0.618
21    L         0.551   0.564   0.589   0.324   0.360   0.435   0.572   0.587   0.618
22    L         0.551   0.564   0.589   0.324   0.360   0.435   0.572   0.587   0.618
23    I         0.551   0.564   0.589   0.659   0.684   0.691   0.572   0.587   0.618
24    S         0.551   0.564   0.589   0.664   0.665   0.694   0.572   0.587   0.618
25    Y         0.551   0.564   0.589   0.755   0.755   0.764   0.572   0.587   0.618
26    T         0.551   0.564   0.589   0.508   0.533   0.575   0.572   0.587   0.618
27    T         0.551   0.564   0.589   0.508   0.533   0.575   0.572   0.587   0.618
28    N        -0.887  -0.707  -0.865  -0.692  -0.438  -0.784  -1.155  -0.872  -1.144
29    Q        -1.141  -0.831  -0.865  -1.454  -0.944  -0.987  -1.587  -1.069  -1.145
30    F         0.551   0.564   0.589   0.697   0.701   0.720   0.572   0.587   0.618
31    P         0.551   0.564   0.589   0.528   0.555   0.590   0.572   0.587   0.618
32    A        -1.141  -1.110  -0.890  -1.401  -1.372  -0.992  -1.587  -1.541  -1.187
33    D        -0.887  -1.031  -0.890  -1.028  -1.307  -1.068  -1.155  -1.404  -1.187
34    Y         0.551   0.564   0.589   0.755   0.755   0.764   0.572   0.587   0.618
35    V         0.551   0.564   0.589   0.186   0.247   0.330   0.572   0.587   0.618
36    P         0.551   0.564   0.589   0.528   0.555   0.590   0.572   0.587   0.618
37    T         0.551   0.564   0.589   0.508   0.533   0.575   0.572   0.587   0.618
38    V         0.551   0.564   0.589   0.186   0.247   0.330   0.572   0.587   0.618
39    F         0.551   0.564   0.589   0.697   0.701   0.720   0.572   0.587   0.618
40    D         0.551   0.564   0.589   0.538   0.549   0.598   0.572   0.587   0.618
41    N         0.551   0.564   0.589   0.863   0.852   0.846   0.572   0.587   0.618
42    Y         0.551   0.564   0.589   0.755   0.755   0.764   0.572   0.587   0.618
43    A         0.551   0.564   0.589   0.596   0.602   0.643   0.572   0.587   0.618
44    V         0.551   0.564   0.589   0.186   0.247   0.330   0.572   0.587   0.618
45    T         0.551   0.564   0.589   0.508   0.533   0.575   0.572   0.587   0.618
46    V         0.551   0.564   0.589   0.186   0.247   0.330   0.572   0.587   0.618
47    M         0.551   0.564   0.589   0.919   0.903   0.889   0.572   0.587   0.618
48    I         0.551   0.564   0.589   0.659   0.684   0.691   0.572   0.587   0.618
49    G         0.551   0.564   0.589   0.620   0.628   0.661   0.572   0.587   0.618
50    D         0.551   0.564   0.589   0.538   0.549   0.598   0.572   0.587   0.618
51    E         0.551   0.564   0.589   0.591   0.605   0.639   0.572   0.587   0.618
52    P         0.551   0.564   0.589   0.528   0.555   0.590   0.572   0.587   0.618
53    Y        -0.887  -0.707  -0.865  -0.785  -0.523  -0.837  -1.155  -0.872  -1.144
54    T         0.551   0.564   0.589   0.508   0.533   0.575   0.572   0.587   0.618
55    L         0.551   0.564   0.589   0.324   0.360   0.435   0.572   0.587   0.618
56    G         0.551   0.564   0.589   0.620   0.628   0.661   0.572   0.587   0.618
57    L         0.551   0.564   0.589   0.324   0.360   0.435   0.572   0.587   0.618
58    F         0.551   0.564   0.589   0.697   0.701   0.720   0.572   0.587   0.618
59    D         0.551   0.564   0.589   0.538   0.549   0.598   0.572   0.587   0.618
60    T         0.551   0.564   0.589   0.508   0.533   0.575   0.572   0.587   0.618
61    A         0.551   0.564   0.589   0.596   0.602   0.643   0.572   0.587   0.618
62    G         0.551   0.564   0.589   0.620   0.628   0.661   0.572   0.587   0.618
63    Q         0.551   0.564   0.589   0.716   0.715   0.734   0.572   0.587   0.618
64    E         0.551   0.564   0.589   0.591   0.605   0.639   0.572   0.587   0.618
65    D         0.551   0.564   0.589   0.538   0.549   0.598   0.572   0.587   0.618
66    Y         0.551   0.564   0.589   0.755   0.755   0.764   0.572   0.587   0.618
67    D         0.551   0.564   0.589   0.538   0.549   0.598   0.572   0.587   0.618
68    R         0.551   0.564   0.589   0.716   0.738   0.734   0.572   0.587   0.618
69    L         0.551   0.564   0.589   0.324   0.360   0.435   0.572   0.587   0.618
70    R         0.551   0.564   0.589   0.716   0.738   0.734   0.572   0.587   0.618
71    P         0.551   0.564   0.589   0.528   0.555   0.590   0.572   0.587   0.618
72    L         0.551   0.564   0.589   0.324   0.360   0.435   0.572   0.587   0.618
73    S        -0.413  -0.417  -0.854  -0.179  -0.195  -0.849  -0.436  -0.442  -1.126
74    Y         0.551   0.564   0.589   0.755   0.755   0.764   0.572   0.587   0.618
75    P         0.551   0.564   0.589   0.528   0.555   0.590   0.572   0.587   0.618
76    S        -1.141  -1.110  -0.890  -1.315  -1.291  -0.926  -1.587  -1.541  -1.187
77    T         0.551   0.564   0.589   0.508   0.533   0.575   0.572   0.587   0.618
78    D         0.551   0.564   0.589   0.538   0.549   0.598   0.572   0.587   0.618
79    V         0.551   0.564   0.589   0.186   0.247   0.330   0.572   0.587   0.618
80    F         0.551   0.564   0.589   0.697   0.701   0.720   0.572   0.587   0.618
81    L         0.551   0.564   0.589   0.324   0.360   0.435   0.572   0.587   0.618
82    V        -0.413  -0.743  -0.863  -0.670  -1.077  -1.212  -0.436  -0.928  -1.142
83    C         0.551   0.564   0.589   0.755   0.759   0.764   0.572   0.587   0.618
84    F         0.551   0.564   0.589   0.697   0.701   0.720   0.572   0.587   0.618
85    S         0.551   0.564   0.589   0.664   0.665   0.694   0.572   0.587   0.618
86    V         0.551   0.564   0.589   0.186   0.247   0.330   0.572   0.587   0.618
87    I        -1.752  -1.968  -1.753  -1.748  -2.099  -1.803  -1.875  -2.104  -1.787
88    S         0.551   0.564   0.589   0.664   0.665   0.694   0.572   0.587   0.618
89    P         0.551   0.564   0.589   0.528   0.555   0.590   0.572   0.587   0.618
90    P        -1.141  -0.831  -0.865  -1.473  -0.916  -1.015  -1.587  -1.069  -1.145
91    S         0.551   0.564   0.589   0.664   0.665   0.694   0.572   0.587   0.618
92    F         0.551   0.564   0.589   0.697   0.701   0.720   0.572   0.587   0.618
93    E         0.551   0.564   0.589   0.591   0.605   0.639   0.572   0.587   0.618
94    N         0.551   0.564   0.589   0.863   0.852   0.846   0.572   0.587   0.618
95    V         0.551   0.564   0.589   0.186   0.247   0.330   0.572   0.587   0.618
96    K        -0.413  -0.743  -0.863  -0.352  -0.821  -1.008  -0.436  -0.928  -1.142
97    E        -0.413  -0.743  -0.863  -0.256  -0.718  -0.929  -0.436  -0.928  -1.142
98    K         0.551   0.564   0.589   0.503   0.515   0.572   0.572   0.587   0.618
99    W         0.551   0.564   0.589   0.974   0.958   0.931   0.572   0.587   0.618
100   F        -0.413  -0.743  -0.863  -0.168  -0.659  -0.916  -0.436  -0.928  -1.142
101   P         0.551   0.564   0.589   0.528   0.555   0.590   0.572   0.587   0.618
102   E         0.551   0.564   0.589   0.591   0.605   0.639   0.572   0.587   0.618
103   V         0.551   0.564   0.589   0.186   0.247   0.330   0.572   0.587   0.618
104   H        -0.413  -0.743  -0.863   0.052  -0.420  -0.683  -0.436  -0.928  -1.142
105   H         0.551   0.564   0.589   0.882   0.875   0.861   0.572   0.587   0.618
106   H         0.551   0.564   0.589   0.882   0.875   0.861   0.572   0.587   0.618
107   C         0.551   0.564   0.589   0.755   0.759   0.764   0.572   0.587   0.618
108   P         0.551   0.564   0.589   0.528   0.555   0.590   0.572   0.587   0.618
109   G         0.551   0.564   0.589   0.620   0.628   0.661   0.572   0.587   0.618
110   V         0.551   0.564   0.589   0.186   0.247   0.330   0.572   0.587   0.618
111   P         0.551   0.564   0.589   0.528   0.555   0.590   0.572   0.587   0.618
112   C        -1.331  -1.493  -1.734  -0.938  -1.215  -1.608  -1.299  -1.533  -1.765
113   L        -0.413  -0.259  -0.848  -0.532  -0.321  -1.101  -0.436  -0.229  -1.116
114   V        -0.887  -0.565  -0.856  -1.037  -0.478  -1.106  -1.155  -0.656  -1.130
115   V         0.551   0.564   0.589   0.186   0.247   0.330   0.572   0.587   0.618
116   G         0.551   0.564   0.589   0.620   0.628   0.661   0.572   0.587   0.618
117   T         0.551   0.564   0.589   0.508   0.533   0.575   0.572   0.587   0.618
118   Q         0.551   0.564   0.589   0.716   0.715   0.734   0.572   0.587   0.618
119   I        -2.828  -2.752  -2.368  -3.210  -3.056  -2.493  -2.739  -2.577  -2.091
120   D         0.551   0.564   0.589   0.538   0.549   0.598   0.572   0.587   0.618
121   L         0.551   0.564   0.589   0.324   0.360   0.435   0.572   0.587   0.618
122   R         0.551   0.564   0.589   0.716   0.738   0.734   0.572   0.587   0.618
123   D        -2.109  -2.012  -1.754  -2.238  -2.123  -1.677  -2.307  -2.168  -1.789
124   D         0.551   0.564   0.589   0.538   0.549   0.598   0.572   0.587   0.618
125   K        -2.552  -2.423  -2.362  -2.635  -2.421  -2.304  -2.451  -2.250  -2.085
126   V        -2.196  -2.446  -2.367  -2.278  -2.602  -2.295  -2.019  -2.277  -2.089
127   I        -1.331  -1.642  -1.741  -1.168  -1.689  -2.028  -1.299  -1.698  -1.773
128   I        -2.661  -2.669  -2.367  -2.841  -2.779  -2.268  -2.595  -2.508  -2.090
129   E        -2.218  -2.063  -1.754  -2.484  -2.263  -1.766  -2.451  -2.223  -1.789
130   K        -0.887  -0.707  -0.865  -1.020  -0.745  -0.986  -1.155  -0.872  -1.144
131   L         0.551   0.564   0.589   0.324   0.360   0.435   0.572   0.587   0.618
132   Q        -3.272  -2.942  -2.843  -3.001  -2.637  -2.383  -2.883  -2.545  -2.271
133   R        -0.413  -0.743  -0.863  -0.132  -0.591  -0.850  -0.436  -0.928  -1.142
134   Q        -1.331  -1.626  -1.740  -0.935  -1.375  -1.541  -1.299  -1.681  -1.773
135   R        -1.942  -1.713  -1.738  -2.080  -1.692  -1.651  -2.163  -1.799  -1.770
136   L        -0.887  -1.036  -0.890  -1.193  -1.424  -1.123  -1.155  -1.413  -1.188
137   R        -2.552  -2.475  -2.364  -2.355  -2.283  -2.102  -2.451  -2.346  -2.087
138   P        -0.413  -0.743  -0.863  -0.290  -0.711  -0.874  -0.436  -0.928  -1.142
139   I        -1.331  -1.358  -1.733  -1.048  -1.081  -1.698  -1.299  -1.324  -1.764
140   T        -1.331  -1.514  -1.734  -1.198  -1.492  -1.784  -1.299  -1.556  -1.765
141   S        -4.769  -4.318  -3.854  -4.195  -3.602  -3.013  -3.459  -3.071  -2.547
142   E        -1.331  -1.514  -1.734  -1.151  -1.472  -1.827  -1.299  -1.556  -1.765
143   Q        -0.413  -0.284  -0.848  -0.103   0.034  -0.736  -0.436  -0.261  -1.117
144   G         0.551   0.564   0.589   0.620   0.628   0.661   0.572   0.587   0.618
145   S        -1.331  -1.128  -1.724  -1.088  -0.839  -1.657  -1.299  -1.049  -1.753
146   R        -1.942  -1.854  -1.749  -2.072  -1.954  -1.726  -2.163  -2.013  -1.783
147   L         0.551   0.564   0.589   0.324   0.360   0.435   0.572   0.587   0.618
148   A         0.551   0.564   0.589   0.596   0.602   0.643   0.572   0.587   0.618
149   R        -1.752  -1.853  -1.745  -1.673  -1.798  -1.704  -1.875  -1.955  -1.778
150   E        -0.413  -0.743  -0.863  -0.282  -0.767  -1.011  -0.436  -0.928  -1.142
151   L         0.551   0.564   0.589   0.324   0.360   0.435   0.572   0.587   0.618
152   K        -2.552  -2.539  -2.368  -2.451  -2.512  -2.258  -2.451  -2.413  -2.090
153   A         0.551   0.564   0.589   0.596   0.602   0.643   0.572   0.587   0.618
154   V         0.551   0.564   0.589   0.186   0.247   0.330   0.572   0.587   0.618
155   K         0.551   0.564   0.589   0.503   0.515   0.572   0.572   0.587   0.618
156   Y         0.551   0.564   0.589   0.755   0.755   0.764   0.572   0.587   0.618
157   V         0.551   0.564   0.589   0.186   0.247   0.330   0.572   0.587   0.618
158   E         0.551   0.564   0.589   0.591   0.605   0.639   0.572   0.587   0.618
159   C         0.551   0.564   0.589   0.755   0.759   0.764   0.572   0.587   0.618
160   S         0.551   0.564   0.589   0.664   0.665   0.694   0.572   0.587   0.618
161   A         0.551   0.564   0.589   0.596   0.602   0.643   0.572   0.587   0.618
162   L         0.551   0.564   0.589   0.324   0.360   0.435   0.572   0.587   0.618
163   T         0.551   0.564   0.589   0.508   0.533   0.575   0.572   0.587   0.618
164   Q         0.551   0.564   0.589   0.716   0.715   0.734   0.572   0.587   0.618
165   R        -1.752  -1.968  -1.753  -1.697  -2.061  -1.778  -1.875  -2.104  -1.787
166   G         0.551   0.564   0.589   0.620   0.628   0.661   0.572   0.587   0.618
167   L         0.551   0.564   0.589   0.324   0.360   0.435   0.572   0.587   0.618
168   K         0.551   0.564   0.589   0.503   0.515   0.572   0.572   0.587   0.618
169   N        -1.752  -1.596  -1.737  -1.567  -1.330  -1.676  -1.875  -1.639  -1.768
170   V         0.551   0.564   0.589   0.186   0.247   0.330   0.572   0.587   0.618
171   F         0.551   0.564   0.589   0.697   0.701   0.720   0.572   0.587   0.618
172   D         0.551   0.564   0.589   0.538   0.549   0.598   0.572   0.587   0.618
173   E         0.551   0.564   0.589   0.591   0.605   0.639   0.572   0.587   0.618
174   A         0.551   0.564   0.589   0.596   0.602   0.643   0.572   0.587   0.618
175   I         0.551   0.564   0.589   0.659   0.684   0.691   0.572   0.587   0.618
176   V         0.551   0.564   0.589   0.186   0.247   0.330   0.572   0.587   0.618
177   A         0.551   0.564   0.589   0.596   0.602   0.643   0.572   0.587   0.618
178   A         0.551   0.564   0.589   0.596   0.602   0.643   0.572   0.587   0.618
179   L         0.551   0.564   0.589   0.324   0.360   0.435   0.572   0.587   0.618
180   E        -0.413  -0.435  -0.854  -0.282  -0.313  -0.993  -0.436  -0.468  -1.127
181   P         0.551   0.564   0.589   0.528   0.555   0.590   0.572   0.587   0.618
182   P         0.551   0.564   0.589   0.528   0.555   0.590   0.572   0.587   0.618
183   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
184   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
185   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
186   V        -0.413  -0.743  -0.863  -0.693  -1.125  -1.287  -0.436  -0.928  -1.142
187   I        -2.196  -2.446  -2.367  -1.969  -2.385  -2.395  -2.019  -2.277  -2.089
188   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
189   K         0.551   0.564   0.589   0.503   0.515   0.572   0.572   0.587   0.618
190   K        -0.413  -0.743  -0.863  -0.344  -0.799  -0.984  -0.436  -0.928  -1.142
191   S        -2.196  -2.446  -2.367  -1.863  -2.307  -2.184  -2.019  -2.277  -2.089
192   K        -0.413  -0.435  -0.854  -0.352  -0.383  -0.993  -0.436  -0.468  -1.127
193   K         0.551   0.564   0.589   0.503   0.515   0.572   0.572   0.587   0.618
194   C         0.551   0.564   0.589   0.755   0.759   0.764   0.572   0.587   0.618
195   A        -2.828  -2.546  -2.368  -3.155  -2.793  -2.398  -2.739  -2.421  -2.091
196   I        -0.887  -0.881  -0.882  -1.037  -1.038  -1.181  -1.155  -1.151  -1.174
197   L         0.551   0.564   0.589   0.324   0.360   0.435   0.572   0.587   0.618
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P19073
Output conservation file - output/P19073
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
