1     M         1.390   1.416   1.497   2.133   2.138   2.289   1.514   1.560   1.695
2     D         1.390   1.416   1.497   1.867   1.886   2.035   1.514   1.560   1.695
3     K         0.536   0.480   0.285   0.653   0.599   0.312   0.565   0.478   0.135
4     I        -0.599  -0.520  -0.615  -0.472  -0.358  -0.423  -0.701  -0.609  -0.702
5     R        -0.032  -0.001   0.120  -0.147  -0.077   0.118  -0.384  -0.345  -0.166
6     E         1.390   1.416   1.497   0.738   0.799   0.956   1.514   1.560   1.695
7     K         1.390   1.416   1.497   1.419   1.472   1.607   1.514   1.560   1.695
8     L        -0.856  -0.788  -0.670  -0.536  -0.406  -0.296  -1.017  -0.928  -0.768
9     S         0.536   0.613   0.302   1.109   1.222   0.727   0.565   0.680   0.164
10    N        -1.012  -1.151  -1.207  -0.411  -0.643  -0.807  -0.859  -1.021  -1.048
11    L         0.536   0.362   0.266   0.629   0.380   0.207   0.565   0.291   0.100
12    K        -0.032  -0.004   0.126  -0.147  -0.049   0.160  -0.384  -0.350  -0.156
13    L         0.536   0.362   0.266   0.629   0.380   0.207   0.565   0.291   0.100
14    E         0.144   0.326   0.256  -0.595  -0.307  -0.387  -0.068   0.231   0.082
15    A         0.144   0.093   0.139   0.395   0.275   0.327  -0.068  -0.172  -0.131
16    E        -0.032  -0.004   0.126  -0.767  -0.566  -0.334  -0.384  -0.350  -0.156
17    S        -1.424  -1.299  -1.229  -2.042  -1.967  -1.799  -1.334  -1.191  -1.071
18    W        -0.032  -0.004   0.126   0.322   0.291   0.474  -0.384  -0.350  -0.156
19    Q        -0.268  -0.482  -0.583   0.286  -0.024  -0.207  -0.226  -0.517  -0.652
20    E         0.144   0.054   0.132  -0.644  -0.736  -0.551  -0.068  -0.243  -0.145
21    K         0.536   0.362   0.266   0.653   0.437   0.282   0.565   0.291   0.100
22    Y        -1.424  -1.359  -1.229  -0.913  -0.841  -0.672  -1.334  -1.246  -1.071
23    E        -0.032   0.076   0.154  -0.767  -0.639  -0.483  -0.384  -0.203  -0.104
24    E         0.536   0.362   0.266  -0.073  -0.294  -0.427   0.565   0.291   0.100
25    L        -0.599  -0.541  -0.615  -0.578  -0.534  -0.542  -0.701  -0.657  -0.702
26    K        -1.836  -1.835  -1.691  -2.134  -2.313  -2.147  -1.492  -1.465  -1.277
27    E        -1.268  -1.313  -1.209  -1.776  -1.791  -1.577  -1.175  -1.194  -1.050
28    K         0.144   0.326   0.256   0.101   0.384   0.267  -0.068   0.231   0.082
29    N        -1.424  -1.416  -1.233  -0.971  -1.000  -0.770  -1.334  -1.300  -1.076
30    K         1.390   1.416   1.497   1.419   1.472   1.607   1.514   1.560   1.695
31    D        -1.012  -0.974  -1.150  -1.495  -1.404  -1.519  -0.859  -0.814  -0.987
32    L        -0.268  -0.382  -0.562  -0.092  -0.237  -0.499  -0.226  -0.391  -0.624
33    E         1.390   1.416   1.497   0.738   0.799   0.956   1.514   1.560   1.695
34    Q         0.536   0.362   0.266   0.947   0.651   0.408   0.565   0.291   0.100
35    E         0.536   0.362   0.266  -0.066  -0.277  -0.408   0.565   0.291   0.100
36    N        -0.599  -0.566  -0.619  -0.472  -0.467  -0.680  -0.701  -0.683  -0.707
37    V        -1.012  -1.031  -1.174  -0.767  -0.793  -0.951  -0.859  -0.891  -1.013
38    E        -1.424  -1.406  -1.236  -1.885  -1.793  -1.630  -1.334  -1.290  -1.080
39    K         0.536   0.636   0.306   0.560   0.722   0.137   0.565   0.712   0.171
40    E        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
41    N        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
42    Q        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
43    I        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
44    K        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
45    S        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
46    L        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
47    T        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
48    V        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
49    K        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
50    N        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
51    Q        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
52    Q        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
53    L        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
54    E        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
55    D        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
56    E        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
57    I        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
58    E        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
59    K        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
60    L        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
61    E        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
62    A        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
63    G        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
64    L        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
65    S        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
66    D        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
67    S        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
68    K        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
69    Q        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
70    T        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
71    E        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
72    Q        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
73    D        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
74    N        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
75    V        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
76    E        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
77    K        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
78    E        -0.032   0.076   0.154  -0.767  -0.639  -0.483  -0.384  -0.203  -0.104
79    N        -0.599  -0.615  -0.644  -0.157  -0.225  -0.249  -0.701  -0.747  -0.738
80    Q        -0.032  -0.004   0.126  -0.577  -0.678  -0.510  -0.384  -0.350  -0.156
81    I        -0.268  -0.351  -0.547   0.575   0.450   0.139  -0.226  -0.353  -0.603
82    K        -1.268  -1.346  -1.227  -1.403  -1.652  -1.545  -1.175  -1.232  -1.070
83    S        -0.032   0.067   0.140   0.099   0.273   0.343  -0.384  -0.220  -0.130
84    L         1.390   1.416   1.497   1.431   1.468   1.618   1.514   1.560   1.695
85    T         0.144   0.054   0.132   0.506   0.321   0.400  -0.068  -0.243  -0.145
86    V        -0.856  -0.788  -0.670  -0.352  -0.273  -0.114  -1.017  -0.928  -0.768
87    K         1.390   1.416   1.497   1.419   1.472   1.607   1.514   1.560   1.695
88    N         0.536   0.362   0.266   1.109   0.872   0.662   0.565   0.291   0.100
89    H        -1.268  -1.301  -1.222  -1.071  -1.265  -1.134  -1.175  -1.199  -1.065
90    Q        -1.680  -1.651  -1.657  -1.095  -1.103  -1.216  -1.334  -1.302  -1.249
91    L         0.536   0.362   0.266   0.629   0.380   0.207   0.565   0.291   0.100
92    E         1.390   1.416   1.497   0.738   0.799   0.956   1.514   1.560   1.695
93    E         0.144   0.054   0.132  -0.590  -0.645  -0.465  -0.068  -0.243  -0.145
94    E         0.144   0.118   0.140  -0.626  -0.629  -0.529  -0.068  -0.127  -0.129
95    I        -0.268  -0.370  -0.550   0.403   0.181  -0.141  -0.226  -0.376  -0.608
96    E         1.390   1.416   1.497   0.738   0.799   0.956   1.514   1.560   1.695
97    K         0.536   0.362   0.266   0.448   0.129  -0.230   0.565   0.291   0.100
98    L         1.390   1.416   1.497   1.431   1.468   1.618   1.514   1.560   1.695
99    E         1.390   1.416   1.497   0.738   0.799   0.956   1.514   1.560   1.695
100   A        -1.680  -1.651  -1.657  -1.376  -1.510  -1.552  -1.334  -1.302  -1.249
101   E        -1.268  -1.059  -1.191  -1.785  -1.522  -1.533  -1.175  -0.966  -1.035
102   L        -0.268  -0.482  -0.583  -0.073  -0.355  -0.488  -0.226  -0.517  -0.652
103   A        -1.836  -1.611  -1.657  -1.583  -1.327  -1.345  -1.492  -1.283  -1.251
104   E         0.536   0.362   0.266  -0.066  -0.277  -0.408   0.565   0.291   0.100
105   S        -1.424  -1.364  -1.216  -1.089  -1.031  -0.839  -1.334  -1.252  -1.060
106   K         0.536   0.362   0.266   0.653   0.437   0.282   0.565   0.291   0.100
107   Q        -1.680  -1.670  -1.661  -1.540  -1.530  -1.753  -1.334  -1.325  -1.252
108   L        -1.268  -1.261  -1.198  -1.120  -1.158  -1.138  -1.175  -1.150  -1.042
109   S         0.144   0.242   0.198   0.391   0.525   0.429  -0.068   0.090  -0.022
110   E        -0.032   0.013   0.126  -0.767  -0.667  -0.462  -0.384  -0.319  -0.155
111   D        -0.599  -0.716  -0.631  -1.230  -1.272  -1.062  -0.701  -0.838  -0.723
112   S        -0.599  -0.719  -0.647  -0.288  -0.576  -0.565  -0.701  -0.843  -0.742
113   H        -1.268  -1.290  -1.221  -0.669  -0.755  -0.689  -1.175  -1.189  -1.064
114   H        -1.836  -1.830  -1.674  -1.165  -1.129  -0.949  -1.492  -1.462  -1.263
115   L        -0.599  -0.615  -0.644  -0.564  -0.586  -0.538  -0.701  -0.747  -0.738
116   Q        -1.268  -1.156  -1.204  -0.989  -0.823  -0.855  -1.175  -1.057  -1.047
117   S        -0.599  -0.483  -0.595  -0.206  -0.029  -0.256  -0.701  -0.540  -0.671
118   N        -1.836  -1.835  -1.691  -1.658  -1.824  -1.637  -1.492  -1.465  -1.277
119   N         0.536   0.362   0.266   1.090   0.838   0.618   0.565   0.291   0.100
120   D         0.536   0.613   0.302  -0.052   0.078  -0.326   0.565   0.680   0.164
121   N        -1.012  -1.151  -1.207  -0.315  -0.531  -0.638  -0.859  -1.021  -1.048
122   F        -0.856  -0.688  -0.631  -0.635  -0.459  -0.346  -1.017  -0.795  -0.718
123   S        -0.032  -0.003   0.121   0.175   0.208   0.377  -0.384  -0.349  -0.165
124   K         0.144   0.054   0.132   0.101  -0.023   0.105  -0.068  -0.243  -0.145
125   K         0.536   0.480   0.285   0.653   0.599   0.312   0.565   0.478   0.135
126   N        -1.268  -1.271  -1.217  -0.916  -0.936  -0.848  -1.175  -1.174  -1.061
127   Q        -0.268  -0.382  -0.562   0.032  -0.266  -0.704  -0.226  -0.391  -0.624
128   Q        -1.424  -1.299  -1.229  -1.194  -1.094  -0.952  -1.334  -1.191  -1.071
129   L         1.390   1.416   1.497   1.431   1.468   1.618   1.514   1.560   1.695
130   E         1.390   1.416   1.497   0.738   0.799   0.956   1.514   1.560   1.695
131   E         1.390   1.416   1.497   0.738   0.799   0.956   1.514   1.560   1.695
132   D         0.144   0.222   0.163  -0.595  -0.453  -0.480  -0.068   0.056  -0.086
133   L         0.536   0.480   0.285   0.629   0.553   0.241   0.565   0.478   0.135
134   E         1.390   1.416   1.497   0.738   0.799   0.956   1.514   1.560   1.695
135   E        -0.268  -0.382  -0.562  -0.797  -0.906  -1.082  -0.226  -0.391  -0.624
136   S        -0.700  -0.760  -0.639  -0.457  -0.519  -0.366  -0.859  -0.903  -0.733
137   D         1.390   1.416   1.497   1.867   1.886   2.035   1.514   1.560   1.695
138   T        -1.012  -0.851  -1.144  -0.816  -0.582  -0.988  -0.859  -0.669  -0.981
139   K        -1.268  -1.346  -1.227  -1.140  -1.267  -1.179  -1.175  -1.232  -1.070
140   L         1.390   1.416   1.497   1.431   1.468   1.618   1.514   1.560   1.695
141   K        -0.032  -0.004   0.126  -0.147  -0.049   0.160  -0.384  -0.350  -0.156
142   E         1.390   1.416   1.497   0.738   0.799   0.956   1.514   1.560   1.695
143   T         1.390   1.416   1.497   1.889   1.910   2.055   1.514   1.560   1.695
144   T         0.536   0.480   0.285   1.106   1.025   0.699   0.565   0.478   0.135
145   E         1.390   1.416   1.497   0.738   0.799   0.956   1.514   1.560   1.695
146   K         1.390   1.416   1.497   1.419   1.472   1.607   1.514   1.560   1.695
147   L         0.536   0.362   0.266   0.696   0.484   0.367   0.565   0.291   0.100
148   R         1.390   1.416   1.497   1.963   1.960   2.126   1.514   1.560   1.695
149   E        -0.032  -0.004   0.126  -0.816  -0.624  -0.390  -0.384  -0.350  -0.156
150   S         0.144   0.242   0.198   0.506   0.656   0.532  -0.068   0.090  -0.022
151   D         1.390   1.416   1.497   1.867   1.886   2.035   1.514   1.560   1.695
152   L        -0.599  -0.426  -0.579  -0.233   0.033  -0.229  -0.701  -0.474  -0.649
153   K         1.390   1.416   1.497   1.419   1.472   1.607   1.514   1.560   1.695
154   A         0.536   0.636   0.306   1.002   1.102   0.665   0.565   0.712   0.171
155   D        -0.599  -0.577  -0.604  -1.148  -1.062  -0.972  -0.701  -0.654  -0.683
156   Q        -0.032  -0.004   0.126   0.252   0.313   0.508  -0.384  -0.350  -0.156
157   L        -0.032  -0.004   0.126  -0.013   0.108   0.315  -0.384  -0.350  -0.156
158   E         1.390   1.416   1.497   0.738   0.799   0.956   1.514   1.560   1.695
159   R         1.390   1.416   1.497   1.963   1.960   2.126   1.514   1.560   1.695
160   R        -0.032  -0.001   0.120  -0.147  -0.077   0.118  -0.384  -0.345  -0.166
161   V         1.390   1.416   1.497   1.999   2.009   2.161   1.514   1.560   1.695
162   A        -0.856  -0.812  -0.685  -0.699  -0.650  -0.472  -1.017  -0.961  -0.787
163   A        -0.032   0.015   0.125   0.099   0.160   0.307  -0.384  -0.315  -0.158
164   L         1.390   1.416   1.497   1.431   1.468   1.618   1.514   1.560   1.695
165   E         1.390   1.416   1.497   0.738   0.799   0.956   1.514   1.560   1.695
166   E        -1.836  -1.835  -1.691  -1.975  -1.880  -1.673  -1.492  -1.465  -1.277
167   Q         0.536   0.613   0.302  -0.066   0.066  -0.356   0.565   0.680   0.164
168   R         0.144   0.326   0.256   0.410   0.724   0.526  -0.068   0.231   0.082
169   E        -0.032   0.081   0.160  -0.516  -0.167  -0.152  -0.384  -0.195  -0.093
170   E        -0.599  -0.676  -0.644  -1.257  -1.251  -1.084  -0.701  -0.802  -0.738
171   W        -0.268  -0.351  -0.547   0.624   0.493   0.132  -0.226  -0.353  -0.603
172   E         1.390   1.416   1.497   0.738   0.799   0.956   1.514   1.560   1.695
173   R        -1.680  -1.696  -1.644  -1.411  -1.406  -1.319  -1.334  -1.345  -1.237
174   K         1.390   1.416   1.497   1.419   1.472   1.607   1.514   1.560   1.695
175   N        -1.836  -1.835  -1.691  -1.080  -1.139  -0.951  -1.492  -1.465  -1.277
176   E         1.390   1.416   1.497   0.738   0.799   0.956   1.514   1.560   1.695
177   E         0.536   0.669   0.310  -0.066   0.136  -0.345   0.565   0.761   0.178
178   L         0.144   0.054   0.132   0.182   0.076   0.225  -0.068  -0.243  -0.145
179   T        -0.268  -0.280  -0.545   0.239   0.209  -0.333  -0.226  -0.245  -0.600
180   V        -1.680  -1.714  -1.670  -2.250  -2.204  -1.998  -1.334  -1.361  -1.260
181   K        -0.268   0.009  -0.502  -0.127   0.255  -0.470  -0.226   0.136  -0.542
182   Y         0.536   0.669   0.310   1.382   1.529   1.034   0.565   0.761   0.178
183   E        -1.268  -1.346  -1.227  -1.399  -1.462  -1.382  -1.175  -1.232  -1.070
184   D        -1.268  -1.298  -1.213  -1.641  -1.812  -1.747  -1.175  -1.195  -1.057
185   A         0.144   0.054   0.132   0.437   0.258   0.382  -0.068  -0.243  -0.145
186   K         0.536   0.480   0.285   0.624   0.568   0.246   0.565   0.478   0.135
187   K        -0.032   0.013   0.126  -0.277  -0.197  -0.029  -0.384  -0.319  -0.155
188   E         0.536   0.480   0.285  -0.050  -0.090  -0.343   0.565   0.478   0.135
189   L         1.390   1.416   1.497   1.431   1.468   1.618   1.514   1.560   1.695
190   D        -0.032   0.018   0.125  -0.516  -0.417  -0.339  -0.384  -0.310  -0.158
191   E         1.390   1.416   1.497   0.738   0.799   0.956   1.514   1.560   1.695
192   I        -0.268  -0.482  -0.583   0.579   0.273   0.091  -0.226  -0.517  -0.652
193   A        -1.836  -1.835  -1.691  -1.288  -1.250  -1.091  -1.492  -1.465  -1.277
194   A        -0.032   0.015   0.125   0.038   0.092   0.252  -0.384  -0.315  -0.158
195   S        -1.268  -1.346  -1.227  -0.889  -1.008  -0.868  -1.175  -1.232  -1.070
196   L         0.536   0.480   0.285   0.682   0.621   0.361   0.565   0.478   0.135
197   E         0.536   0.480   0.285  -0.073  -0.124  -0.399   0.565   0.478   0.135
198   N        -1.268  -1.243  -1.200  -0.847  -0.776  -0.772  -1.175  -1.127  -1.041
199   L        -0.032  -0.001   0.120   0.051   0.059   0.200  -0.384  -0.345  -0.166
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P17536
Output conservation file - output/P17536
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
