1     M         1.128   1.191   1.250   1.337   1.432   1.505   1.197   1.276   1.371
2     S        -0.291  -0.609  -0.627  -0.213  -0.638  -0.720  -0.290  -0.697  -0.734
3     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
4     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
5     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
6     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
7     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
8     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
9     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
10    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
11    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
12    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
13    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
14    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
15    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
16    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
17    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
18    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
19    R        -0.291  -0.386  -0.592  -0.074  -0.227  -0.588  -0.290  -0.413  -0.687
20    P         1.128   1.191   1.250   1.224   1.311   1.403   1.197   1.276   1.371
21    I         0.044  -0.092   0.025  -0.004  -0.273  -0.107  -0.176  -0.427  -0.268
22    V         1.128   1.191   1.250   1.059   1.142   1.254   1.197   1.276   1.371
23    I         0.402   0.243   0.145   0.506   0.305   0.125   0.397   0.150  -0.050
24    S         0.402   0.243   0.145   0.411   0.237   0.062   0.397   0.150  -0.050
25    G         1.128   1.191   1.250   1.023   1.111   1.222   1.197   1.276   1.371
26    P         1.128   1.191   1.250   1.224   1.311   1.403   1.197   1.276   1.371
27    S         1.128   1.191   1.250   1.026   1.139   1.225   1.197   1.276   1.371
28    G         1.128   1.191   1.250   1.023   1.111   1.222   1.197   1.276   1.371
29    T         0.044  -0.092   0.025  -0.032  -0.294  -0.128  -0.176  -0.427  -0.268
30    G         1.128   1.191   1.250   1.023   1.111   1.222   1.197   1.276   1.371
31    K         1.128   1.191   1.250   0.950   1.051   1.156   1.197   1.276   1.371
32    S         0.402   0.249   0.147   0.397   0.218   0.026   0.397   0.159  -0.047
33    T         1.128   1.191   1.250   1.101   1.203   1.293   1.197   1.276   1.371
34    L         1.128   1.191   1.250   1.044   1.126   1.241   1.197   1.276   1.371
35    L         0.402   0.249   0.147   0.429   0.232   0.080   0.397   0.159  -0.047
36    K         1.128   1.191   1.250   0.950   1.051   1.156   1.197   1.276   1.371
37    K        -0.608  -0.783  -0.669  -0.716  -0.962  -0.780  -0.748  -0.944  -0.794
38    L         1.128   1.191   1.250   1.044   1.126   1.241   1.197   1.276   1.371
39    F         0.044   0.004   0.037  -0.016  -0.100  -0.095  -0.176  -0.254  -0.246
40    A        -0.291  -0.609  -0.627  -0.240  -0.722  -0.801  -0.290  -0.697  -0.734
41    E        -0.291  -0.609  -0.627  -0.237  -0.678  -0.700  -0.290  -0.697  -0.734
42    Y        -0.877  -0.852  -0.704  -0.741  -0.686  -0.516  -1.091  -1.036  -0.836
43    P         0.402   0.243   0.145   0.595   0.380   0.191   0.397   0.150  -0.050
44    D         0.402   0.573   0.171   0.459   0.692   0.140   0.397   0.639  -0.004
45    S        -1.546  -1.444  -1.214  -1.715  -1.552  -1.235  -1.549  -1.387  -1.112
46    F         0.402   0.243   0.145   0.497   0.296   0.114   0.397   0.150  -0.050
47    G         0.402   0.249   0.147   0.405   0.212   0.053   0.397   0.159  -0.047
48    F         0.402   0.249   0.147   0.497   0.304   0.116   0.397   0.159  -0.047
49    S         1.128   1.191   1.250   1.026   1.139   1.225   1.197   1.276   1.371
50    V         0.402   0.511   0.168   0.445   0.585   0.129   0.397   0.554  -0.010
51    S         1.128   1.191   1.250   1.026   1.139   1.225   1.197   1.276   1.371
52    S        -1.419  -1.309  -1.206  -1.271  -1.037  -0.914  -1.434  -1.269  -1.104
53    T         1.128   1.191   1.250   1.101   1.203   1.293   1.197   1.276   1.371
54    T         1.128   1.191   1.250   1.101   1.203   1.293   1.197   1.276   1.371
55    R         1.128   1.191   1.250   1.189   1.277   1.372   1.197   1.276   1.371
56    T        -1.880  -1.808  -1.598  -1.872  -1.763  -1.481  -1.663  -1.529  -1.268
57    P         1.128   1.191   1.250   1.224   1.311   1.403   1.197   1.276   1.371
58    R         1.128   1.191   1.250   1.189   1.277   1.372   1.197   1.276   1.371
59    A        -1.293  -1.133  -1.157  -1.229  -1.029  -1.009  -1.320  -1.116  -1.059
60    G         1.128   1.191   1.250   1.023   1.111   1.222   1.197   1.276   1.371
61    E         1.128   1.191   1.250   0.979   1.054   1.183   1.197   1.276   1.371
62    V        -0.608  -0.657  -0.667  -0.693  -0.860  -0.825  -0.748  -0.834  -0.792
63    N        -0.751  -0.511  -0.631  -0.735  -0.390  -0.502  -0.976  -0.626  -0.745
64    G         1.128   1.191   1.250   1.023   1.111   1.222   1.197   1.276   1.371
65    K        -0.608  -0.785  -0.667  -0.751  -1.004  -0.823  -0.748  -0.946  -0.792
66    D         0.402   0.510   0.171   0.441   0.603   0.097   0.397   0.553  -0.004
67    Y         1.128   1.191   1.250   1.259   1.353   1.434   1.197   1.276   1.371
68    N         0.044  -0.092   0.025   0.202   0.001   0.150  -0.176  -0.427  -0.268
69    F         0.402   0.249   0.147   0.527   0.357   0.197   0.397   0.159  -0.047
70    V         0.044   0.202   0.088  -0.084   0.158  -0.052  -0.176   0.083  -0.152
71    S         0.402   0.604   0.176   0.473   0.740   0.143   0.397   0.681   0.004
72    V         0.044  -0.092   0.025  -0.108  -0.398  -0.223  -0.176  -0.427  -0.268
73    D        -0.148  -0.140   0.014  -0.440  -0.475  -0.223  -0.519  -0.516  -0.288
74    E        -0.942  -0.976  -1.131  -0.841  -0.927  -1.116  -0.862  -0.879  -1.024
75    F         1.128   1.191   1.250   1.123   1.222   1.312   1.197   1.276   1.371
76    K        -0.291  -0.609  -0.627  -0.271  -0.698  -0.733  -0.290  -0.697  -0.734
77    S        -1.419  -1.436  -1.205  -1.547  -1.592  -1.246  -1.434  -1.380  -1.103
78    M        -0.751  -0.564  -0.631  -0.769  -0.519  -0.595  -0.976  -0.684  -0.743
79    I        -0.291  -0.609  -0.627  -0.130  -0.593  -0.691  -0.290  -0.697  -0.734
80    K        -1.419  -1.274  -1.205  -1.622  -1.426  -1.327  -1.434  -1.247  -1.103
81    N        -1.754  -1.642  -1.575  -1.733  -1.610  -1.507  -1.549  -1.378  -1.248
82    N        -1.546  -1.433  -1.218  -1.757  -1.617  -1.285  -1.549  -1.378  -1.115
83    E        -0.942  -0.900  -1.122  -0.929  -0.889  -1.237  -0.862  -0.800  -1.015
84    F         1.128   1.191   1.250   1.123   1.222   1.312   1.197   1.276   1.371
85    I         0.044  -0.092   0.025  -0.004  -0.273  -0.107  -0.176  -0.427  -0.268
86    E         1.128   1.191   1.250   0.979   1.054   1.183   1.197   1.276   1.371
87    W         0.402   0.249   0.147   0.765   0.569   0.339   0.397   0.159  -0.047
88    A         0.402   0.510   0.171   0.416   0.561   0.103   0.397   0.553  -0.004
89    Q         0.402   0.243   0.145   0.634   0.423   0.233   0.397   0.150  -0.050
90    F         1.128   1.191   1.250   1.123   1.222   1.312   1.197   1.276   1.371
91    S         0.044   0.007   0.038  -0.117  -0.178  -0.177  -0.176  -0.249  -0.244
92    G         0.402   0.249   0.147   0.394   0.197   0.025   0.397   0.159  -0.047
93    N         1.128   1.191   1.250   1.241   1.350   1.419   1.197   1.276   1.371
94    Y        -0.291  -0.609  -0.627   0.071  -0.340  -0.415  -0.290  -0.697  -0.734
95    Y         1.128   1.191   1.250   1.259   1.353   1.434   1.197   1.276   1.371
96    G         1.128   1.191   1.250   1.023   1.111   1.222   1.197   1.276   1.371
97    S         0.402   0.604   0.176   0.473   0.740   0.143   0.397   0.681   0.004
98    T        -0.751  -0.655  -0.655  -0.917  -0.777  -0.766  -0.976  -0.831  -0.777
99    V        -1.293  -1.295  -1.172  -1.339  -1.338  -1.155  -1.320  -1.255  -1.072
100   A        -1.754  -1.634  -1.574  -1.754  -1.528  -1.475  -1.549  -1.380  -1.248
101   S        -1.293  -1.286  -1.174  -1.422  -1.373  -1.219  -1.320  -1.244  -1.073
102   V         0.044  -0.092   0.025  -0.050  -0.300  -0.118  -0.176  -0.427  -0.268
103   K        -0.942  -1.174  -1.165  -0.871  -1.198  -1.187  -0.862  -1.104  -1.061
104   Q        -1.754  -1.447  -1.556  -1.773  -1.350  -1.483  -1.549  -1.194  -1.232
105   V        -0.291  -0.609  -0.627  -0.152  -0.590  -0.624  -0.290  -0.697  -0.734
106   S        -1.880  -1.810  -1.599  -1.949  -1.859  -1.544  -1.663  -1.531  -1.269
107   K        -2.214  -1.926  -1.922  -2.225  -1.772  -1.799  -1.777  -1.472  -1.381
108   S        -1.754  -1.717  -1.591  -1.713  -1.690  -1.446  -1.549  -1.460  -1.263
109   G         0.044   0.004   0.037  -0.048  -0.109  -0.061  -0.176  -0.254  -0.246
110   K        -0.608  -0.423  -0.617  -0.673  -0.403  -0.636  -0.748  -0.468  -0.723
111   T        -1.546  -1.645  -1.585  -1.298  -1.506  -1.410  -1.320  -1.383  -1.257
112   C        -0.291  -0.609  -0.627   0.146  -0.325  -0.456  -0.290  -0.697  -0.734
113   I        -0.291  -0.321  -0.577  -0.115  -0.169  -0.565  -0.290  -0.335  -0.667
114   L         1.128   1.191   1.250   1.044   1.126   1.241   1.197   1.276   1.371
115   D         1.128   1.191   1.250   1.076   1.183   1.270   1.197   1.276   1.371
116   I         1.128   1.191   1.250   1.130   1.227   1.318   1.197   1.276   1.371
117   D         0.044   0.007   0.038  -0.081  -0.160  -0.162  -0.176  -0.249  -0.243
118   M         0.402   0.243   0.145   0.711   0.503   0.294   0.397   0.150  -0.050
119   Q        -0.291  -0.382  -0.586   0.006  -0.141  -0.496  -0.290  -0.406  -0.680
120   G         1.128   1.191   1.250   1.023   1.111   1.222   1.197   1.276   1.371
121   V         1.128   1.191   1.250   1.059   1.142   1.254   1.197   1.276   1.371
122   K         0.402   0.243   0.145   0.323   0.126  -0.033   0.397   0.150  -0.050
123   S        -0.959  -0.906  -0.708  -1.167  -1.022  -0.763  -1.205  -1.097  -0.841
124   V         0.402   0.249   0.147   0.445   0.249   0.095   0.397   0.159  -0.047
125   K         0.402   0.249   0.147   0.355   0.189   0.054   0.397   0.159  -0.047
126   A        -1.211  -0.913  -1.138  -1.237  -0.806  -1.107  -1.205  -0.823  -1.035
127   I        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
128   P        -1.211  -0.855  -1.132  -1.217  -0.688  -1.099  -1.205  -0.754  -1.028
129   E        -1.546  -1.630  -1.583  -1.305  -1.441  -1.394  -1.320  -1.370  -1.254
130   L        -0.291  -0.609  -0.627  -0.175  -0.613  -0.657  -0.290  -0.697  -0.734
131   N        -0.942  -1.173  -1.168  -0.595  -0.919  -1.014  -0.862  -1.103  -1.064
132   A         1.128   1.191   1.250   1.033   1.123   1.231   1.197   1.276   1.371
133   R         0.402   0.243   0.145   0.593   0.402   0.250   0.397   0.150  -0.050
134   F        -0.209  -0.104   0.028  -0.342  -0.145   0.032  -0.633  -0.449  -0.261
135   L         0.044  -0.092   0.025  -0.087  -0.362  -0.170  -0.176  -0.427  -0.268
136   F         1.128   1.191   1.250   1.123   1.222   1.312   1.197   1.276   1.371
137   I        -0.959  -0.911  -0.706  -1.178  -1.115  -0.804  -1.205  -1.103  -0.838
138   A         0.044   0.202   0.088  -0.108   0.138  -0.073  -0.176   0.083  -0.152
139   P         1.128   1.191   1.250   1.224   1.311   1.403   1.197   1.276   1.371
140   P         1.128   1.191   1.250   1.224   1.311   1.403   1.197   1.276   1.371
141   S         1.128   1.191   1.250   1.026   1.139   1.225   1.197   1.276   1.371
142   V        -1.211  -1.118  -1.152  -1.109  -0.987  -1.021  -1.205  -1.048  -1.048
143   E         0.402   0.573   0.169   0.358   0.561   0.044   0.397   0.639  -0.009
144   D        -1.754  -1.645  -1.573  -1.714  -1.540  -1.427  -1.549  -1.382  -1.247
145   L         1.128   1.191   1.250   1.044   1.126   1.241   1.197   1.276   1.371
146   K        -0.877  -0.868  -0.707  -1.142  -1.169  -0.883  -1.091  -1.055  -0.840
147   K        -2.214  -2.092  -1.922  -2.228  -2.055  -1.806  -1.777  -1.611  -1.380
148   R         1.128   1.191   1.250   1.189   1.277   1.372   1.197   1.276   1.371
149   L         1.128   1.191   1.250   1.044   1.126   1.241   1.197   1.276   1.371
150   E        -1.419  -1.275  -1.202  -1.438  -1.224  -1.098  -1.434  -1.244  -1.101
151   G         1.128   1.191   1.250   1.023   1.111   1.222   1.197   1.276   1.371
152   R         1.128   1.191   1.250   1.189   1.277   1.372   1.197   1.276   1.371
153   G         1.128   1.191   1.250   1.023   1.111   1.222   1.197   1.276   1.371
154   T         1.128   1.191   1.250   1.101   1.203   1.293   1.197   1.276   1.371
155   E         0.402   0.249   0.147   0.358   0.151   0.007   0.397   0.159  -0.047
156   T        -0.291  -0.609  -0.627  -0.127  -0.553  -0.637  -0.290  -0.697  -0.734
157   E        -1.211  -0.966  -1.141  -1.200  -0.871  -1.047  -1.205  -0.880  -1.038
158   E        -0.608  -0.646  -0.634  -0.736  -0.780  -0.782  -0.748  -0.748  -0.746
159   S        -0.291  -0.609  -0.627  -0.217  -0.644  -0.730  -0.290  -0.697  -0.734
160   I        -0.209  -0.108   0.026  -0.493  -0.315  -0.112  -0.633  -0.456  -0.266
161   N        -1.419  -1.250  -1.198  -1.466  -1.291  -1.116  -1.434  -1.225  -1.097
162   K         0.402   0.243   0.145   0.355   0.181   0.051   0.397   0.150  -0.050
163   R         1.128   1.191   1.250   1.189   1.277   1.372   1.197   1.276   1.371
164   L         0.044   0.202   0.088  -0.064   0.165  -0.017  -0.176   0.083  -0.152
165   S        -1.211  -1.150  -1.155  -1.234  -1.156  -1.148  -1.205  -1.087  -1.052
166   A        -0.291  -0.609  -0.627  -0.152  -0.563  -0.584  -0.290  -0.697  -0.734
167   A         1.128   1.191   1.250   1.033   1.123   1.231   1.197   1.276   1.371
168   Q        -2.548  -2.325  -2.199  -2.380  -2.061  -1.897  -1.892  -1.659  -1.462
169   A        -0.942  -0.921  -1.124  -0.782  -0.748  -1.056  -0.862  -0.821  -1.017
170   E         1.128   1.191   1.250   0.979   1.054   1.183   1.197   1.276   1.371
171   L        -0.751  -0.787  -0.655  -0.750  -0.788  -0.587  -0.976  -0.950  -0.777
172   A        -0.751  -0.661  -0.651  -1.006  -0.899  -0.818  -0.976  -0.841  -0.775
173   Y         0.044   0.007   0.038   0.140   0.067   0.053  -0.176  -0.249  -0.243
174   A        -0.148  -0.141   0.015  -0.453  -0.462  -0.216  -0.519  -0.518  -0.286
175   E        -1.293  -1.089  -1.160  -1.423  -1.190  -1.144  -1.320  -1.080  -1.061
176   T        -0.942  -0.930  -1.129  -0.792  -0.796  -1.160  -0.862  -0.832  -1.023
177   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
178   G         1.128   1.191   1.250   1.023   1.111   1.222   1.197   1.276   1.371
179   A        -0.291  -0.609  -0.627  -0.177  -0.602  -0.644  -0.290  -0.697  -0.734
180   H         0.402   0.243   0.145   0.755   0.546   0.350   0.397   0.150  -0.050
181   D         1.128   1.191   1.250   1.076   1.183   1.270   1.197   1.276   1.371
182   K        -1.211  -1.206  -1.162  -1.304  -1.291  -1.209  -1.205  -1.143  -1.059
183   V        -1.211  -1.149  -1.156  -1.149  -1.058  -1.095  -1.205  -1.086  -1.053
184   I         0.402   0.573   0.171   0.506   0.729   0.170   0.397   0.639  -0.004
185   V         1.128   1.191   1.250   1.059   1.142   1.254   1.197   1.276   1.371
186   N         1.128   1.191   1.250   1.241   1.350   1.419   1.197   1.276   1.371
187   D         0.402   0.249   0.147   0.441   0.249   0.053   0.397   0.159  -0.047
188   D         1.128   1.191   1.250   1.076   1.183   1.270   1.197   1.276   1.371
189   L         0.402   0.243   0.145   0.420   0.205   0.053   0.397   0.150  -0.050
190   D         0.044  -0.092   0.025  -0.081  -0.360  -0.192  -0.176  -0.427  -0.268
191   K        -0.291  -0.327  -0.582  -0.289  -0.334  -0.706  -0.290  -0.342  -0.673
192   A         1.128   1.191   1.250   1.033   1.123   1.231   1.197   1.276   1.371
193   Y         1.128   1.191   1.250   1.259   1.353   1.434   1.197   1.276   1.371
194   K        -1.546  -1.192  -1.527  -1.468  -0.973  -1.465  -1.320  -0.903  -1.205
195   E        -0.608  -0.540  -0.644  -0.678  -0.615  -0.724  -0.748  -0.674  -0.762
196   L        -0.148  -0.020   0.041  -0.392  -0.190  -0.104  -0.519  -0.296  -0.239
197   K         0.044  -0.092   0.025  -0.206  -0.478  -0.287  -0.176  -0.427  -0.268
198   D        -1.754  -1.789  -1.597  -1.614  -1.714  -1.483  -1.549  -1.512  -1.267
199   F         0.402   0.243   0.145   0.509   0.320   0.146   0.397   0.150  -0.050
200   I        -0.291  -0.609  -0.627  -0.056  -0.461  -0.509  -0.290  -0.697  -0.734
201   F        -1.419  -1.436  -1.203  -1.456  -1.457  -1.131  -1.434  -1.380  -1.102
202   A        -1.754  -1.651  -1.575  -1.819  -1.661  -1.568  -1.549  -1.389  -1.248
203   E        -0.942  -1.128  -1.163  -0.795  -1.075  -1.070  -0.862  -1.060  -1.059
204   K        -1.976  -1.825  -1.621  -1.970  -1.747  -1.475  -1.732  -1.529  -1.286
205   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
206   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
207   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
208   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
209   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
210   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P15454
Output conservation file - output/P15454
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
