1     M         0.948   0.950   0.981   1.163   1.168   1.198   1.007   1.011   1.054
2     S         0.948   0.950   0.981   0.866   0.874   0.927   1.007   1.011   1.054
3     G         0.075   0.141  -0.098   0.013   0.103  -0.262  -0.037   0.065  -0.365
4     L        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
5     R        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
6     T        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
7     V        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
8     S        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
9     A        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
10    S        -1.375  -1.406  -1.280  -1.440  -1.490  -1.293  -1.344  -1.378  -1.217
11    S        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
12    G        -1.774  -1.784  -1.644  -1.686  -1.656  -1.483  -1.532  -1.544  -1.368
13    N        -1.279  -1.185  -1.245  -1.260  -1.125  -1.168  -1.258  -1.151  -1.182
14    G        -1.772  -1.796  -1.632  -1.776  -1.787  -1.538  -1.544  -1.555  -1.359
15    K        -0.261  -0.248  -0.149  -0.445  -0.424  -0.267  -0.625  -0.608  -0.459
16    S        -1.167  -1.199  -1.235  -1.148  -1.197  -1.239  -1.135  -1.171  -1.174
17    Y         0.948   0.950   0.981   1.124   1.125   1.162   1.007   1.011   1.054
18    D         0.948   0.950   0.981   0.931   0.935   0.986   1.007   1.011   1.054
19    S        -1.658  -1.718  -1.636  -1.417  -1.499  -1.363  -1.442  -1.489  -1.362
20    I        -0.751  -0.742  -0.780  -0.920  -0.921  -0.927  -0.931  -0.931  -0.929
21    M        -0.206  -0.234  -0.147  -0.216  -0.297  -0.187  -0.523  -0.581  -0.455
22    K         0.948   0.950   0.981   0.887   0.896   0.946   1.007   1.011   1.054
23    I        -0.206  -0.154  -0.122  -0.452  -0.361  -0.324  -0.523  -0.436  -0.409
24    L         0.948   0.950   0.981   0.899   0.900   0.956   1.007   1.011   1.054
25    L         0.948   0.950   0.981   0.899   0.900   0.956   1.007   1.011   1.054
26    I        -0.622  -0.565  -0.744  -0.674  -0.590  -0.860  -0.727  -0.650  -0.877
27    G         0.948   0.950   0.981   0.852   0.858   0.913   1.007   1.011   1.054
28    D         0.948   0.950   0.981   0.931   0.935   0.986   1.007   1.011   1.054
29    S         0.948   0.950   0.981   0.866   0.874   0.927   1.007   1.011   1.054
30    G         0.948   0.950   0.981   0.852   0.858   0.913   1.007   1.011   1.054
31    V         0.948   0.950   0.981   0.945   0.951   0.999   1.007   1.011   1.054
32    G         0.948   0.950   0.981   0.852   0.858   0.913   1.007   1.011   1.054
33    K         0.948   0.950   0.981   0.887   0.896   0.946   1.007   1.011   1.054
34    S         0.948   0.950   0.981   0.866   0.874   0.927   1.007   1.011   1.054
35    C         0.948   0.950   0.981   1.149   1.149   1.185   1.007   1.011   1.054
36    L         0.291   0.280  -0.041   0.372   0.357  -0.051   0.293   0.275  -0.263
37    L         0.948   0.950   0.981   0.899   0.900   0.956   1.007   1.011   1.054
38    V        -0.206  -0.154  -0.122  -0.407  -0.320  -0.278  -0.523  -0.436  -0.409
39    R         0.948   0.950   0.981   1.040   1.039   1.085   1.007   1.011   1.054
40    F         0.948   0.950   0.981   1.011   1.014   1.059   1.007   1.011   1.054
41    V        -0.622  -0.725  -0.771  -0.574  -0.721  -0.779  -0.727  -0.863  -0.914
42    E         0.948   0.950   0.981   0.934   0.936   0.988   1.007   1.011   1.054
43    D         0.948   0.950   0.981   0.931   0.935   0.986   1.007   1.011   1.054
44    K        -0.622  -0.725  -0.771  -0.645  -0.792  -0.853  -0.727  -0.863  -0.914
45    F         0.948   0.950   0.981   1.011   1.014   1.059   1.007   1.011   1.054
46    N        -0.261  -0.239  -0.146  -0.483  -0.449  -0.298  -0.625  -0.592  -0.452
47    P         0.948   0.950   0.981   1.188   1.191   1.220   1.007   1.011   1.054
48    S         0.291   0.312  -0.038   0.307   0.336  -0.163   0.293   0.322  -0.256
49    F         0.948   0.950   0.981   1.011   1.014   1.059   1.007   1.011   1.054
50    I         0.948   0.950   0.981   0.861   0.866   0.922   1.007   1.011   1.054
51    T         0.948   0.950   0.981   0.928   0.931   0.983   1.007   1.011   1.054
52    T         0.948   0.950   0.981   0.928   0.931   0.983   1.007   1.011   1.054
53    I         0.948   0.950   0.981   0.861   0.866   0.922   1.007   1.011   1.054
54    G         0.948   0.950   0.981   0.852   0.858   0.913   1.007   1.011   1.054
55    I         0.948   0.950   0.981   0.861   0.866   0.922   1.007   1.011   1.054
56    D         0.948   0.950   0.981   0.931   0.935   0.986   1.007   1.011   1.054
57    F         0.948   0.950   0.981   1.011   1.014   1.059   1.007   1.011   1.054
58    K         0.948   0.950   0.981   0.887   0.896   0.946   1.007   1.011   1.054
59    I         0.948   0.950   0.981   0.861   0.866   0.922   1.007   1.011   1.054
60    K        -0.206  -0.154  -0.122  -0.312  -0.218  -0.199  -0.523  -0.436  -0.409
61    T         0.948   0.950   0.981   0.928   0.931   0.983   1.007   1.011   1.054
62    V        -0.206  -0.154  -0.122  -0.440  -0.352  -0.308  -0.523  -0.436  -0.409
63    D        -0.261  -0.249  -0.150  -0.492  -0.478  -0.313  -0.625  -0.610  -0.460
64    I        -0.865  -0.853  -0.790  -1.077  -1.065  -0.977  -1.033  -1.023  -0.938
65    N        -1.167  -1.222  -1.247  -1.118  -1.217  -1.277  -1.135  -1.202  -1.184
66    G         0.291   0.195  -0.047   0.295   0.177  -0.183   0.293   0.148  -0.273
67    K         0.291   0.195  -0.047   0.349   0.237  -0.102   0.293   0.148  -0.273
68    K        -0.206  -0.148  -0.128  -0.312  -0.206  -0.213  -0.523  -0.425  -0.421
69    V        -0.206  -0.195  -0.140  -0.410  -0.395  -0.326  -0.523  -0.511  -0.442
70    K         0.948   0.950   0.981   0.887   0.896   0.946   1.007   1.011   1.054
71    L         0.948   0.950   0.981   0.899   0.900   0.956   1.007   1.011   1.054
72    Q         0.948   0.950   0.981   1.071   1.072   1.114   1.007   1.011   1.054
73    L        -0.939  -0.934  -0.807  -1.151  -1.149  -0.953  -1.135  -1.130  -0.959
74    W         0.948   0.950   0.981   1.193   1.195   1.225   1.007   1.011   1.054
75    D         0.948   0.950   0.981   0.931   0.935   0.986   1.007   1.011   1.054
76    T         0.948   0.950   0.981   0.928   0.931   0.983   1.007   1.011   1.054
77    A         0.948   0.950   0.981   0.974   0.978   1.025   1.007   1.011   1.054
78    G         0.948   0.950   0.981   0.852   0.858   0.913   1.007   1.011   1.054
79    Q         0.948   0.950   0.981   1.071   1.072   1.114   1.007   1.011   1.054
80    E         0.948   0.950   0.981   0.934   0.936   0.988   1.007   1.011   1.054
81    R         0.948   0.950   0.981   1.040   1.039   1.085   1.007   1.011   1.054
82    F         0.948   0.950   0.981   1.011   1.014   1.059   1.007   1.011   1.054
83    R         0.948   0.950   0.981   1.040   1.039   1.085   1.007   1.011   1.054
84    T         0.948   0.950   0.981   0.928   0.931   0.983   1.007   1.011   1.054
85    I         0.948   0.950   0.981   0.861   0.866   0.922   1.007   1.011   1.054
86    T         0.948   0.950   0.981   0.928   0.931   0.983   1.007   1.011   1.054
87    T         0.948   0.950   0.981   0.928   0.931   0.983   1.007   1.011   1.054
88    A         0.948   0.950   0.981   0.974   0.978   1.025   1.007   1.011   1.054
89    Y         0.948   0.950   0.981   1.124   1.125   1.162   1.007   1.011   1.054
90    Y         0.948   0.950   0.981   1.124   1.125   1.162   1.007   1.011   1.054
91    R         0.948   0.950   0.981   1.040   1.039   1.085   1.007   1.011   1.054
92    G         0.948   0.950   0.981   0.852   0.858   0.913   1.007   1.011   1.054
93    A         0.948   0.950   0.981   0.974   0.978   1.025   1.007   1.011   1.054
94    M         0.948   0.950   0.981   1.163   1.168   1.198   1.007   1.011   1.054
95    G         0.948   0.950   0.981   0.852   0.858   0.913   1.007   1.011   1.054
96    I         0.948   0.950   0.981   0.861   0.866   0.922   1.007   1.011   1.054
97    I        -0.939  -0.885  -0.793  -1.151  -1.070  -0.929  -1.135  -1.072  -0.943
98    L         0.948   0.950   0.981   0.899   0.900   0.956   1.007   1.011   1.054
99    V        -0.751  -0.767  -0.773  -0.883  -0.894  -0.899  -0.931  -0.940  -0.919
100   Y         0.948   0.950   0.981   1.124   1.125   1.162   1.007   1.011   1.054
101   D         0.948   0.950   0.981   0.931   0.935   0.986   1.007   1.011   1.054
102   V         0.948   0.950   0.981   0.945   0.951   0.999   1.007   1.011   1.054
103   T         0.948   0.950   0.981   0.928   0.931   0.983   1.007   1.011   1.054
104   D         0.948   0.950   0.981   0.931   0.935   0.986   1.007   1.011   1.054
105   E        -0.622  -0.629  -0.750  -0.636  -0.637  -0.857  -0.727  -0.727  -0.886
106   R        -0.033  -0.111  -0.124   0.010  -0.135  -0.204  -0.217  -0.357  -0.413
107   T        -0.261  -0.241  -0.148  -0.543  -0.514  -0.358  -0.625  -0.595  -0.456
108   F         0.948   0.950   0.981   1.011   1.014   1.059   1.007   1.011   1.054
109   T        -1.658  -1.619  -1.621  -1.539  -1.510  -1.496  -1.442  -1.417  -1.350
110   N         0.948   0.950   0.981   0.948   0.956   1.002   1.007   1.011   1.054
111   I        -0.206  -0.195  -0.140  -0.440  -0.426  -0.339  -0.523  -0.511  -0.442
112   K        -0.939  -0.892  -0.789  -1.048  -0.980  -0.828  -1.135  -1.083  -0.939
113   Q        -0.939  -0.934  -0.807  -0.999  -1.005  -0.822  -1.135  -1.130  -0.959
114   W         0.948   0.950   0.981   1.193   1.195   1.225   1.007   1.011   1.054
115   F        -0.033  -0.066  -0.123   0.058   0.003  -0.097  -0.217  -0.279  -0.410
116   K        -1.772  -1.736  -1.626  -1.716  -1.661  -1.502  -1.544  -1.513  -1.354
117   T        -0.206  -0.234  -0.147  -0.379  -0.438  -0.300  -0.523  -0.581  -0.455
118   V         0.948   0.950   0.981   0.945   0.951   0.999   1.007   1.011   1.054
119   N        -1.355  -1.389  -1.273  -1.349  -1.391  -1.208  -1.340  -1.367  -1.210
120   E        -0.622  -0.633  -0.737  -0.484  -0.489  -0.704  -0.727  -0.726  -0.868
121   H         0.291   0.195  -0.047   0.679   0.561   0.188   0.293   0.148  -0.273
122   A         0.948   0.950   0.981   0.974   0.978   1.025   1.007   1.011   1.054
123   N        -0.622  -0.645  -0.763  -0.614  -0.656  -0.860  -0.727  -0.764  -0.905
124   D        -0.033   0.050  -0.066  -0.079   0.044  -0.155  -0.217  -0.083  -0.308
125   E        -1.355  -1.362  -1.261  -1.435  -1.453  -1.295  -1.340  -1.342  -1.198
126   A        -0.261  -0.239  -0.146  -0.451  -0.418  -0.269  -0.625  -0.592  -0.452
127   Q        -1.469  -1.563  -1.614  -1.059  -1.208  -1.328  -1.237  -1.335  -1.343
128   L        -1.355  -1.329  -1.264  -1.422  -1.393  -1.242  -1.340  -1.315  -1.201
129   L        -0.939  -0.897  -0.800  -1.139  -1.085  -0.920  -1.135  -1.085  -0.950
130   L         0.948   0.950   0.981   0.899   0.900   0.956   1.007   1.011   1.054
131   V        -0.033  -0.067  -0.110  -0.090  -0.153  -0.262  -0.217  -0.282  -0.386
132   G         0.948   0.950   0.981   0.852   0.858   0.913   1.007   1.011   1.054
133   N         0.948   0.950   0.981   0.948   0.956   1.002   1.007   1.011   1.054
134   K         0.948   0.950   0.981   0.887   0.896   0.946   1.007   1.011   1.054
135   S        -0.622  -0.534  -0.741  -0.430  -0.290  -0.671  -0.727  -0.604  -0.873
136   D         0.948   0.950   0.981   0.931   0.935   0.986   1.007   1.011   1.054
137   M        -1.658  -1.718  -1.636  -1.253  -1.341  -1.234  -1.442  -1.489  -1.362
138   -         0.075   0.012  -0.096   0.065  -0.032  -0.225  -0.037  -0.146  -0.362
139   E        -0.751  -0.712  -0.759  -0.839  -0.793  -0.826  -0.931  -0.890  -0.903
140   T        -1.355  -1.374  -1.272  -1.378  -1.407  -1.233  -1.340  -1.355  -1.209
141   R         0.948   0.950   0.981   1.040   1.039   1.085   1.007   1.011   1.054
142   V        -1.241  -1.225  -1.250  -1.126  -1.109  -1.136  -1.237  -1.221  -1.189
143   V         0.948   0.950   0.981   0.945   0.951   0.999   1.007   1.011   1.054
144   T        -0.033   0.050  -0.066  -0.146  -0.018  -0.213  -0.217  -0.083  -0.308
145   A        -1.658  -1.671  -1.622  -1.456  -1.487  -1.405  -1.442  -1.452  -1.351
146   D         0.291   0.363  -0.031   0.375   0.458  -0.092   0.293   0.394  -0.244
147   Q         0.948   0.950   0.981   1.071   1.072   1.114   1.007   1.011   1.054
148   G         0.948   0.950   0.981   0.852   0.858   0.913   1.007   1.011   1.054
149   E        -0.206  -0.154  -0.122  -0.260  -0.170  -0.151  -0.523  -0.436  -0.409
150   A        -0.033   0.014  -0.087  -0.039   0.031  -0.158  -0.217  -0.143  -0.344
151   L         0.948   0.950   0.981   0.899   0.900   0.956   1.007   1.011   1.054
152   A         0.948   0.950   0.981   0.974   0.978   1.025   1.007   1.011   1.054
153   K        -1.241  -1.175  -1.254  -1.285  -1.190  -1.282  -1.237  -1.172  -1.192
154   E        -0.206  -0.207  -0.143  -0.407  -0.416  -0.325  -0.523  -0.531  -0.447
155   L         0.291   0.195  -0.047   0.352   0.230  -0.117   0.293   0.148  -0.273
156   G        -0.033   0.014  -0.087  -0.153  -0.080  -0.252  -0.217  -0.143  -0.344
157   I        -0.622  -0.575  -0.745  -0.674  -0.622  -0.864  -0.727  -0.681  -0.882
158   P         0.291   0.236  -0.040   0.623   0.552   0.091   0.293   0.210  -0.261
159   F         0.948   0.950   0.981   1.011   1.014   1.059   1.007   1.011   1.054
160   I        -0.206  -0.154  -0.122  -0.452  -0.361  -0.324  -0.523  -0.436  -0.409
161   E         0.948   0.950   0.981   0.934   0.936   0.988   1.007   1.011   1.054
162   S        -0.622  -0.561  -0.729  -0.586  -0.482  -0.762  -0.727  -0.637  -0.857
163   S         0.948   0.950   0.981   0.866   0.874   0.927   1.007   1.011   1.054
164   A         0.291   0.195  -0.047   0.407   0.281  -0.112   0.293   0.148  -0.273
165   K         0.948   0.950   0.981   0.887   0.896   0.946   1.007   1.011   1.054
166   N        -1.772  -1.735  -1.633  -1.759  -1.706  -1.548  -1.544  -1.510  -1.360
167   D        -0.206  -0.154  -0.122  -0.370  -0.277  -0.248  -0.523  -0.436  -0.409
168   D        -1.772  -1.754  -1.637  -1.780  -1.764  -1.582  -1.544  -1.524  -1.363
169   N         0.948   0.950   0.981   0.948   0.956   1.002   1.007   1.011   1.054
170   V         0.291   0.280  -0.041   0.377   0.364  -0.128   0.293   0.275  -0.263
171   N        -1.355  -1.321  -1.261  -1.406  -1.363  -1.279  -1.340  -1.307  -1.199
172   E        -0.335  -0.264  -0.714  -0.144  -0.062  -0.668  -0.319  -0.230  -0.837
173   I        -0.622  -0.725  -0.771  -0.613  -0.753  -0.782  -0.727  -0.863  -0.914
174   F         0.948   0.950   0.981   1.011   1.014   1.059   1.007   1.011   1.054
175   F        -0.206  -0.207  -0.143  -0.213  -0.222  -0.127  -0.523  -0.531  -0.447
176   T        -0.865  -0.866  -0.794  -1.001  -1.020  -0.922  -1.033  -1.041  -0.943
177   L         0.291   0.195  -0.047   0.342   0.218  -0.144   0.293   0.148  -0.273
178   A         0.948   0.950   0.981   0.974   0.978   1.025   1.007   1.011   1.054
179   K        -2.074  -2.062  -1.925  -1.937  -1.924  -1.754  -1.646  -1.634  -1.459
180   L        -1.658  -1.718  -1.636  -1.598  -1.683  -1.549  -1.442  -1.489  -1.362
181   I         0.948   0.950   0.981   0.861   0.866   0.922   1.007   1.011   1.054
182   Q        -0.622  -0.725  -0.771  -0.469  -0.630  -0.725  -0.727  -0.863  -0.914
183   E        -0.622  -0.725  -0.771  -0.620  -0.781  -0.869  -0.727  -0.863  -0.914
184   K        -0.924  -1.054  -1.228  -0.716  -0.895  -1.133  -0.829  -0.985  -1.164
185   I        -1.167  -1.240  -1.243  -1.044  -1.147  -1.153  -1.135  -1.205  -1.180
186   D        -1.355  -1.389  -1.273  -1.417  -1.470  -1.291  -1.340  -1.367  -1.210
187   S        -1.658  -1.625  -1.614  -1.606  -1.554  -1.550  -1.442  -1.407  -1.344
188   N        -1.658  -1.612  -1.610  -1.565  -1.495  -1.514  -1.442  -1.394  -1.341
189   K        -1.167  -1.151  -1.242  -1.156  -1.141  -1.278  -1.135  -1.124  -1.180
190   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
191   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
192   L        -0.719  -0.624  -0.762  -0.798  -0.654  -0.808  -0.858  -0.725  -0.901
193   V        -2.074  -2.005  -1.922  -1.938  -1.839  -1.746  -1.646  -1.591  -1.456
194   G        -0.751  -0.730  -0.777  -0.878  -0.864  -0.855  -0.931  -0.920  -0.925
195   V        -2.678  -2.633  -2.412  -2.334  -2.296  -1.995  -1.850  -1.819  -1.596
196   G        -1.279  -1.209  -1.257  -1.299  -1.232  -1.291  -1.258  -1.204  -1.195
197   N        -1.279  -1.299  -1.250  -1.348  -1.385  -1.307  -1.258  -1.281  -1.189
198   G        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
199   K        -1.241  -1.233  -1.247  -1.320  -1.299  -1.283  -1.237  -1.226  -1.186
200   E        -1.355  -1.307  -1.265  -1.397  -1.347  -1.274  -1.340  -1.299  -1.203
201   G        -0.335  -0.396  -0.718  -0.267  -0.347  -0.839  -0.319  -0.404  -0.843
202   N        -0.261  -0.248  -0.149  -0.539  -0.520  -0.357  -0.625  -0.608  -0.459
203   I        -0.261  -0.249  -0.150  -0.535  -0.516  -0.350  -0.625  -0.610  -0.459
204   S        -0.939  -0.941  -0.808  -1.142  -1.142  -0.936  -1.135  -1.139  -0.961
205   I        -0.751  -0.773  -0.777  -0.900  -0.926  -0.912  -0.931  -0.949  -0.924
206   N        -1.658  -1.688  -1.623  -1.586  -1.629  -1.526  -1.442  -1.462  -1.351
207   S        -2.376  -2.355  -2.192  -2.084  -2.077  -1.902  -1.748  -1.732  -1.541
208   G        -1.279  -1.242  -1.237  -1.342  -1.300  -1.298  -1.258  -1.226  -1.176
209   S        -2.100  -2.055  -1.926  -1.967  -1.917  -1.728  -1.658  -1.624  -1.459
210   G        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
211   N        -1.355  -1.371  -1.255  -1.440  -1.457  -1.284  -1.340  -1.347  -1.195
212   S        -1.658  -1.678  -1.607  -1.632  -1.649  -1.532  -1.442  -1.454  -1.338
213   S        -0.924  -0.906  -1.212  -0.762  -0.746  -1.178  -0.829  -0.814  -1.148
214   K        -0.924  -1.054  -1.228  -0.770  -0.954  -1.246  -0.829  -0.985  -1.164
215   S        -0.924  -0.947  -1.206  -0.808  -0.845  -1.262  -0.829  -0.861  -1.141
216   N        -0.622  -0.711  -0.772  -0.585  -0.725  -0.816  -0.727  -0.851  -0.915
217   C         0.948   0.950   0.981   1.149   1.149   1.185   1.007   1.011   1.054
218   C         0.948   0.950   0.981   1.149   1.149   1.185   1.007   1.011   1.054
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P07560
Output conservation file - output/P07560
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
