1     M         1.357   1.412   1.516   1.598   1.665   1.820   1.466   1.545   1.717
2     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
3     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
4     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
5     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
6     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
7     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
8     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
9     -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
10    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
11    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
12    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
13    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
14    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
15    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
16    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
17    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
18    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
19    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
20    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
21    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
22    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
23    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
24    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
25    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
26    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
27    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
28    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
29    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
30    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
31    S        -0.800  -0.677  -0.868  -0.520  -0.344  -0.651  -0.779  -0.621  -0.823
32    K        -1.911  -1.637  -1.667  -1.563  -1.150  -1.203  -1.528  -1.250  -1.210
33    N        -1.541  -1.370  -1.331  -1.491  -1.254  -1.170  -1.378  -1.195  -1.082
34    R        -0.132   0.088  -0.299  -0.012   0.273  -0.260  -0.180   0.129  -0.421
35    -        -1.541  -1.481  -1.356  -1.393  -1.305  -1.064  -1.378  -1.288  -1.103
36    -        -1.541  -1.547  -1.362  -1.390  -1.420  -1.135  -1.378  -1.339  -1.107
37    -         0.099   0.125   0.269   0.028   0.046   0.273  -0.294  -0.267  -0.057
38    -        -1.134  -1.073  -0.940  -1.029  -0.906  -0.737  -1.174  -1.088  -0.904
39    -        -1.652  -1.561  -1.379  -1.526  -1.380  -1.113  -1.468  -1.349  -1.121
40    -        -0.615  -0.545  -0.425  -0.781  -0.673  -0.484  -0.881  -0.773  -0.604
41    -         1.357   1.412   1.516   1.668   1.732   1.886   1.466   1.545   1.717
42    -        -1.031  -0.946  -0.915  -0.982  -0.855  -0.788  -1.079  -0.962  -0.881
43    -         0.238   0.407   0.363   0.115   0.386   0.292  -0.031   0.255   0.124
44    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
45    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
46    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
47    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
48    S        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
49    S        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
50    L        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
51    Q        -1.561  -1.376  -1.357  -1.537  -1.213  -1.244  -1.386  -1.173  -1.103
52    S        -1.561  -1.499  -1.363  -1.407  -1.257  -1.119  -1.386  -1.297  -1.108
53    G        -0.697  -0.611  -0.459  -0.821  -0.695  -0.450  -0.979  -0.852  -0.646
54    P        -1.911  -1.819  -1.690  -1.725  -1.676  -1.441  -1.528  -1.417  -1.228
55    L        -0.800  -0.897  -0.912  -0.971  -1.155  -1.152  -0.779  -0.890  -0.873
56    N        -1.761  -1.701  -1.660  -1.512  -1.454  -1.365  -1.399  -1.322  -1.204
57    S        -2.207  -2.126  -1.974  -1.933  -1.792  -1.603  -1.628  -1.527  -1.318
58    E        -0.018  -0.157  -0.321  -0.124  -0.298  -0.519  -0.024  -0.224  -0.453
59    L         0.652   0.677   0.453   0.550   0.598   0.257   0.664   0.698   0.292
60    L        -0.587  -0.604  -0.458  -0.825  -0.812  -0.552  -0.826  -0.844  -0.645
61    E        -0.650  -0.726  -0.875  -0.807  -0.945  -1.149  -0.597  -0.694  -0.831
62    E         0.652   0.658   0.446   0.445   0.481   0.101   0.664   0.669   0.279
63    Q         1.357   1.412   1.516   1.545   1.602   1.771   1.466   1.545   1.717
64    K         0.120   0.161   0.275  -0.070  -0.019   0.142  -0.253  -0.196  -0.046
65    Q        -0.018  -0.127  -0.321   0.255   0.084  -0.260  -0.024  -0.186  -0.453
66    E        -0.018  -0.199  -0.329  -0.203  -0.455  -0.716  -0.024  -0.277  -0.464
67    I         0.652   0.475   0.416   0.768   0.542   0.446   0.664   0.373   0.225
68    Y        -1.883  -1.885  -1.695  -1.615  -1.614  -1.327  -1.514  -1.464  -1.232
69    E         1.357   1.412   1.516   1.125   1.224   1.377   1.466   1.545   1.717
70    A         1.357   1.412   1.516   1.442   1.501   1.674   1.466   1.545   1.717
71    F         1.357   1.412   1.516   1.459   1.523   1.690   1.466   1.545   1.717
72    S        -0.911  -0.911  -0.921  -0.855  -0.947  -0.931  -0.941  -0.942  -0.888
73    L         1.357   1.412   1.516   1.200   1.283   1.447   1.466   1.545   1.717
74    F         1.357   1.412   1.516   1.459   1.523   1.690   1.466   1.545   1.717
75    D         1.357   1.412   1.516   1.182   1.266   1.430   1.466   1.545   1.717
76    M        -0.326  -0.415  -0.405  -0.125  -0.294  -0.287  -0.482  -0.615  -0.580
77    N        -0.018  -0.036  -0.302   0.222   0.233  -0.224  -0.024  -0.041  -0.426
78    N        -0.911  -0.921  -0.921  -0.787  -0.812  -0.793  -0.941  -0.950  -0.889
79    D         0.652   0.658   0.446   0.549   0.578   0.269   0.664   0.669   0.279
80    G        -0.018  -0.036  -0.302   0.227   0.202  -0.225  -0.024  -0.041  -0.426
81    F        -1.559  -1.503  -1.358  -1.344  -1.339  -1.078  -1.399  -1.311  -1.105
82    L         0.652   0.677   0.453   0.550   0.598   0.257   0.664   0.698   0.292
83    D         0.652   0.475   0.416   0.559   0.336   0.249   0.664   0.373   0.225
84    Y         0.652   0.658   0.446   1.030   1.047   0.691   0.664   0.669   0.279
85    H         0.652   0.475   0.416   1.022   0.743   0.551   0.664   0.373   0.225
86    E         0.652   0.658   0.446   0.503   0.543   0.248   0.664   0.669   0.279
87    L         0.306   0.273   0.293   0.053  -0.008   0.014   0.091   0.014  -0.010
88    K         0.120   0.122   0.271  -0.070  -0.113   0.127  -0.253  -0.273  -0.053
89    V        -0.326  -0.482  -0.407  -0.150  -0.415  -0.335  -0.482  -0.675  -0.580
90    A         0.652   0.475   0.416   0.826   0.579   0.497   0.664   0.373   0.225
91    M        -0.587  -0.521  -0.432  -0.687  -0.615  -0.521  -0.826  -0.731  -0.612
92    K         0.652   0.817   0.469   0.751   0.949   0.485   0.664   0.905   0.322
93    A         1.357   1.412   1.516   1.442   1.501   1.674   1.466   1.545   1.717
94    L         1.357   1.412   1.516   1.200   1.283   1.447   1.466   1.545   1.717
95    G         1.357   1.412   1.516   1.500   1.565   1.729   1.466   1.545   1.717
96    F         1.357   1.412   1.516   1.459   1.523   1.690   1.466   1.545   1.717
97    E        -0.326  -0.262  -0.356  -0.713  -0.561  -0.746  -0.482  -0.375  -0.509
98    L         0.652   0.677   0.453   0.559   0.605   0.278   0.664   0.698   0.292
99    P        -1.437  -1.411  -1.331  -1.202  -1.158  -1.060  -1.284  -1.229  -1.082
100   K         1.357   1.412   1.516   1.467   1.547   1.698   1.466   1.545   1.717
101   R        -0.911  -0.878  -0.918  -0.887  -0.893  -0.861  -0.941  -0.913  -0.886
102   E        -0.326  -0.273  -0.383  -0.627  -0.544  -0.694  -0.482  -0.445  -0.551
103   I        -0.326  -0.354  -0.361  -0.151  -0.156  -0.223  -0.482  -0.488  -0.512
104   L         0.652   0.475   0.416   0.561   0.323   0.223   0.664   0.373   0.225
105   D        -0.650  -0.574  -0.846  -0.743  -0.605  -1.044  -0.597  -0.492  -0.798
106   L         0.061   0.121   0.269  -0.408  -0.307  -0.064  -0.368  -0.275  -0.057
107   I         0.120   0.122   0.271  -0.244  -0.293  -0.065  -0.253  -0.273  -0.053
108   D        -0.911  -0.968  -0.913  -1.014  -1.096  -0.972  -0.941  -0.969  -0.877
109   E        -0.911  -0.912  -0.909  -1.257  -1.248  -1.223  -0.941  -0.931  -0.876
110   Y        -0.911  -0.966  -0.921  -0.551  -0.624  -0.583  -0.941  -0.990  -0.889
111   D         0.652   0.475   0.416   0.550   0.317   0.224   0.664   0.373   0.225
112   S        -1.761  -1.701  -1.660  -1.389  -1.323  -1.256  -1.399  -1.322  -1.204
113   E        -0.911  -0.951  -0.925  -1.123  -1.148  -1.118  -0.941  -0.978  -0.892
114   G        -1.437  -1.469  -1.343  -1.398  -1.386  -1.207  -1.284  -1.273  -1.092
115   R        -1.235  -1.342  -1.334  -1.028  -1.220  -1.148  -1.055  -1.147  -1.082
116   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
117   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
118   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
119   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
120   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
121   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
122   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
123   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
124   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
125   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
126   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
127   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
128   H        -1.113  -0.960  -0.930  -1.126  -0.925  -0.800  -1.170  -0.988  -0.896
129   L        -0.650  -0.646  -0.857  -0.581  -0.551  -0.780  -0.597  -0.574  -0.811
130   M        -0.911  -1.006  -0.925  -0.910  -1.047  -0.936  -0.941  -1.016  -0.891
131   K        -1.113  -1.126  -0.945  -1.036  -1.035  -0.772  -1.170  -1.140  -0.912
132   Y        -0.650  -0.836  -0.904  -0.178  -0.484  -0.597  -0.597  -0.812  -0.864
133   D        -0.465  -0.534  -0.396  -0.963  -0.960  -0.741  -0.711  -0.754  -0.568
134   D        -0.911  -0.981  -0.920  -1.062  -1.112  -0.996  -0.941  -0.982  -0.885
135   F         0.652   0.475   0.416   0.818   0.555   0.446   0.664   0.373   0.225
136   Y        -1.437  -1.499  -1.344  -1.203  -1.298  -1.100  -1.284  -1.298  -1.092
137   I        -1.761  -1.682  -1.653  -1.589  -1.498  -1.490  -1.399  -1.306  -1.199
138   V        -1.235  -1.204  -1.302  -0.930  -0.925  -1.128  -1.055  -1.015  -1.053
139   M        -0.465  -0.516  -0.394  -0.356  -0.417  -0.238  -0.711  -0.727  -0.564
140   G         0.306   0.317   0.319   0.379   0.378   0.357   0.091   0.095   0.040
141   E        -0.018  -0.215  -0.340  -0.106  -0.346  -0.503  -0.024  -0.298  -0.480
142   K        -0.911  -0.990  -0.918  -0.851  -1.006  -0.892  -0.941  -1.005  -0.885
143   I         0.652   0.725   0.455   0.766   0.870   0.502   0.664   0.771   0.296
144   L        -0.911  -0.944  -0.913  -1.044  -1.092  -0.997  -0.941  -0.953  -0.878
145   K        -1.437  -1.379  -1.329  -1.452  -1.374  -1.329  -1.284  -1.205  -1.080
146   R         1.357   1.412   1.516   1.388   1.448   1.623   1.466   1.545   1.717
147   D         1.357   1.412   1.516   1.182   1.266   1.430   1.466   1.545   1.717
148   P         1.357   1.412   1.516   1.686   1.746   1.903   1.466   1.545   1.717
149   L        -0.018  -0.157  -0.321  -0.037  -0.225  -0.428  -0.024  -0.224  -0.453
150   D         0.120   0.286   0.334  -0.493  -0.150  -0.126  -0.253   0.037   0.069
151   E         1.357   1.412   1.516   1.125   1.224   1.377   1.466   1.545   1.717
152   I        -0.018  -0.215  -0.340   0.102  -0.202  -0.411  -0.024  -0.298  -0.480
153   K         0.120   0.151   0.273  -0.070  -0.043   0.136  -0.253  -0.217  -0.050
154   R         1.357   1.412   1.516   1.388   1.448   1.623   1.466   1.545   1.717
155   A         1.357   1.412   1.516   1.442   1.501   1.674   1.466   1.545   1.717
156   F         1.357   1.412   1.516   1.459   1.523   1.690   1.466   1.545   1.717
157   Q        -0.990  -0.878  -0.898  -1.070  -0.934  -0.935  -1.055  -0.916  -0.866
158   L         1.357   1.412   1.516   1.200   1.283   1.447   1.466   1.545   1.717
159   F         1.357   1.412   1.516   1.459   1.523   1.690   1.466   1.545   1.717
160   D         1.357   1.412   1.516   1.182   1.266   1.430   1.466   1.545   1.717
161   D        -0.911  -0.932  -0.915  -1.217  -1.246  -1.213  -0.941  -0.955  -0.883
162   D         0.652   0.725   0.455   0.549   0.662   0.284   0.664   0.771   0.296
163   H        -0.911  -0.777  -0.887  -0.813  -0.619  -0.785  -0.941  -0.768  -0.851
164   T        -0.018  -0.157  -0.321   0.259   0.043  -0.283  -0.024  -0.224  -0.453
165   G         0.652   0.725   0.455   0.863   0.956   0.560   0.664   0.771   0.296
166   K        -0.018  -0.215  -0.340   0.211  -0.054  -0.279  -0.024  -0.298  -0.480
167   I         1.357   1.412   1.516   1.384   1.463   1.619   1.466   1.545   1.717
168   S         0.120   0.125   0.270   0.106   0.087   0.313  -0.253  -0.267  -0.056
169   I        -1.113  -1.140  -0.949  -1.304  -1.297  -0.988  -1.170  -1.155  -0.916
170   K        -0.990  -1.033  -0.919  -1.095  -1.190  -0.968  -1.055  -1.061  -0.886
171   N         0.306   0.240   0.290   0.249   0.108   0.080   0.091  -0.047  -0.016
172   L         1.357   1.412   1.516   1.200   1.283   1.447   1.466   1.545   1.717
173   R         0.652   0.812   0.465   0.751   0.943   0.477   0.664   0.897   0.314
174   R        -0.326  -0.256  -0.369  -0.415  -0.312  -0.535  -0.482  -0.379  -0.530
175   V         0.652   0.760   0.462   0.964   1.106   0.646   0.664   0.823   0.309
176   A         0.306   0.253   0.290   0.321   0.210   0.256   0.091  -0.022  -0.016
177   K        -0.911  -0.765  -0.882  -0.797  -0.601  -0.720  -0.941  -0.760  -0.847
178   E        -0.018  -0.127  -0.321  -0.175  -0.299  -0.634  -0.024  -0.186  -0.453
179   L         0.652   0.475   0.416   0.585   0.365   0.286   0.664   0.373   0.225
180   G         0.306   0.317   0.319   0.354   0.363   0.318   0.091   0.095   0.040
181   E         0.652   0.475   0.416   0.491   0.283   0.170   0.664   0.373   0.225
182   T         0.652   0.817   0.469   0.869   1.101   0.611   0.664   0.905   0.322
183   L         0.120   0.122   0.271  -0.311  -0.307  -0.052  -0.253  -0.273  -0.053
184   T        -0.911  -0.988  -0.932  -0.795  -0.926  -0.828  -0.941  -1.011  -0.898
185   D        -0.018   0.007  -0.292  -0.116  -0.069  -0.534  -0.024   0.018  -0.412
186   E        -1.235  -1.302  -1.324  -1.357  -1.425  -1.438  -1.055  -1.112  -1.072
187   E         0.652   0.475   0.416   0.445   0.197   0.044   0.664   0.373   0.225
188   L        -0.018  -0.157  -0.321  -0.029  -0.227  -0.410  -0.024  -0.224  -0.453
189   R        -1.437  -1.485  -1.339  -1.351  -1.410  -1.163  -1.284  -1.291  -1.089
190   A         1.357   1.412   1.516   1.442   1.501   1.674   1.466   1.545   1.717
191   M         1.357   1.412   1.516   1.598   1.665   1.820   1.466   1.545   1.717
192   I         1.357   1.412   1.516   1.384   1.463   1.619   1.466   1.545   1.717
193   E         0.061   0.139   0.284  -0.621  -0.460  -0.234  -0.368  -0.239  -0.028
194   E         1.357   1.412   1.516   1.125   1.224   1.377   1.466   1.545   1.717
195   F         1.357   1.412   1.516   1.459   1.523   1.690   1.466   1.545   1.717
196   D         1.357   1.412   1.516   1.182   1.266   1.430   1.466   1.545   1.717
197   L        -0.018  -0.157  -0.321  -0.044  -0.243  -0.445  -0.024  -0.224  -0.453
198   D         0.652   0.475   0.416   0.559   0.336   0.249   0.664   0.373   0.225
199   G        -1.235  -1.151  -0.972  -1.618  -1.416  -1.160  -1.284  -1.168  -0.938
200   D         0.652   0.475   0.416   0.545   0.313   0.211   0.664   0.373   0.225
201   G         1.357   1.412   1.516   1.500   1.565   1.729   1.466   1.545   1.717
202   E        -0.326  -0.370  -0.396  -0.593  -0.653  -0.637  -0.482  -0.567  -0.569
203   I         0.652   0.475   0.416   0.768   0.542   0.446   0.664   0.373   0.225
204   N         0.652   0.475   0.416   0.815   0.564   0.379   0.664   0.373   0.225
205   E         0.652   0.658   0.446   0.447   0.483   0.108   0.664   0.669   0.279
206   N        -0.911  -0.904  -0.898  -1.170  -1.181  -1.111  -0.941  -0.916  -0.862
207   E         1.357   1.412   1.516   1.125   1.224   1.377   1.466   1.545   1.717
208   F         1.357   1.412   1.516   1.459   1.523   1.690   1.466   1.545   1.717
209   I         0.652   0.475   0.416   0.709   0.442   0.288   0.664   0.373   0.225
210   A        -0.911  -0.824  -0.903  -0.865  -0.796  -0.840  -0.941  -0.851  -0.870
211   I         0.652   0.475   0.416   0.709   0.442   0.288   0.664   0.373   0.225
212   C         0.306   0.222   0.285   0.612   0.446   0.489   0.091  -0.080  -0.026
213   T         0.120   0.127   0.269   0.128   0.109   0.336  -0.253  -0.263  -0.057
214   D        -0.465  -0.544  -0.404  -0.980  -1.018  -0.805  -0.711  -0.770  -0.580
215   S        -0.966  -0.950  -0.913  -0.889  -0.885  -0.868  -0.979  -0.953  -0.874
216   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P06704
Output conservation file - output/P06704
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
