1     M         1.036   1.065   1.124   1.459   1.477   1.525   1.064   1.115   1.198
2     A         0.052  -0.189  -0.241   0.164  -0.219  -0.360   0.047  -0.318  -0.434
3     K         1.036   1.065   1.124   0.639   0.730   0.858   1.064   1.115   1.198
4     Q        -2.135  -1.990  -1.708  -1.881  -1.768  -1.355  -1.923  -1.752  -1.386
5     S         0.132  -0.061  -0.209   0.005  -0.213  -0.351   0.155  -0.123  -0.382
6     L        -2.551  -2.577  -2.179  -2.153  -2.118  -1.626  -2.053  -2.023  -1.570
7     D         0.132  -0.061  -0.209   0.407   0.107  -0.241   0.155  -0.123  -0.382
8     V         1.036   1.065   1.124   0.907   0.946   1.076   1.064   1.115   1.198
9     S         0.132   0.064  -0.202   0.005  -0.059  -0.342   0.155   0.057  -0.371
10    S         1.036   1.065   1.124   0.736   0.794   0.937   1.064   1.115   1.198
11    D        -0.552  -0.275  -0.241  -0.962  -0.549  -0.453  -0.884  -0.454  -0.435
12    R         1.036   1.065   1.124   0.893   0.925   1.065   1.064   1.115   1.198
13    R        -0.314  -0.074  -0.233  -0.616  -0.251  -0.548  -0.494  -0.144  -0.422
14    K         1.036   1.065   1.124   0.639   0.730   0.858   1.064   1.115   1.198
15    A        -0.730  -0.926  -1.069  -0.471  -0.764  -1.047  -0.624  -0.872  -1.035
16    R         1.036   1.065   1.124   0.893   0.925   1.065   1.064   1.115   1.198
17    K         1.036   1.065   1.124   0.639   0.730   0.858   1.064   1.115   1.198
18    A         1.036   1.065   1.124   1.079   1.121   1.216   1.064   1.115   1.198
19    Y        -0.628  -0.437  -0.266  -0.415  -0.131   0.061  -1.014  -0.721  -0.477
20    F         1.036   1.065   1.124   1.428   1.453   1.500   1.064   1.115   1.198
21    T        -1.303  -1.254  -1.091  -1.311  -1.357  -1.068  -1.403  -1.319  -1.064
22    A         0.132   0.294  -0.185   0.258   0.460  -0.303   0.155   0.368  -0.343
23    P        -0.628  -0.517  -0.300  -0.958  -0.770  -0.435  -1.014  -0.859  -0.534
24    S         1.036   1.065   1.124   0.736   0.794   0.937   1.064   1.115   1.198
25    S        -1.125  -1.042  -1.078  -1.446  -1.294  -1.338  -1.143  -1.018  -1.047
26    Q        -1.125  -1.020  -1.096  -0.933  -0.947  -0.975  -1.143  -1.092  -1.070
27    R         1.036   1.065   1.124   0.893   0.925   1.065   1.064   1.115   1.198
28    R         1.036   1.065   1.124   0.893   0.925   1.065   1.064   1.115   1.198
29    V        -1.541  -1.682  -1.683  -1.294  -1.507  -1.460  -1.273  -1.441  -1.362
30    L         0.132  -0.061  -0.209   0.217   0.009  -0.248   0.155  -0.123  -0.382
31    L        -0.628  -0.437  -0.266  -0.685  -0.288  -0.108  -1.014  -0.721  -0.477
32    S         1.036   1.065   1.124   0.736   0.794   0.937   1.064   1.115   1.198
33    A         1.036   1.065   1.124   1.079   1.121   1.216   1.064   1.115   1.198
34    P         1.036   1.065   1.124   1.316   1.347   1.409   1.064   1.115   1.198
35    L         1.036   1.065   1.124   0.974   1.059   1.130   1.064   1.115   1.198
36    S         1.036   1.065   1.124   0.736   0.794   0.937   1.064   1.115   1.198
37    K         1.036   1.065   1.124   0.639   0.730   0.858   1.064   1.115   1.198
38    E         1.036   1.065   1.124   1.215   1.256   1.326   1.064   1.115   1.198
39    L         1.036   1.065   1.124   0.974   1.059   1.130   1.064   1.115   1.198
40    R         1.036   1.065   1.124   0.893   0.925   1.065   1.064   1.115   1.198
41    A        -1.125  -0.699  -1.039  -0.870  -0.296  -0.786  -1.143  -0.619  -0.999
42    Q        -0.314  -0.444  -0.297  -0.214  -0.539  -0.456  -0.494  -0.732  -0.527
43    Y         1.036   1.065   1.124   1.397   1.425   1.475   1.064   1.115   1.198
44    G        -1.562  -1.321  -1.108  -1.758  -1.467  -1.116  -1.663  -1.389  -1.083
45    I        -0.552  -0.458  -0.296  -0.728  -0.613  -0.397  -0.884  -0.757  -0.526
46    K        -0.552  -0.517  -0.291  -1.242  -1.161  -0.735  -0.884  -0.858  -0.518
47    A        -0.552  -0.275  -0.241  -1.047  -0.603  -0.523  -0.884  -0.454  -0.435
48    L        -0.314  -0.332  -0.286  -0.386  -0.399  -0.367  -0.494  -0.547  -0.509
49    P         1.036   1.065   1.124   1.316   1.347   1.409   1.064   1.115   1.198
50    I         0.132   0.064  -0.202   0.398   0.291  -0.186   0.155   0.057  -0.371
51    R         1.036   1.065   1.124   0.893   0.925   1.065   1.064   1.115   1.198
52    R        -1.562  -1.329  -1.111  -2.046  -1.744  -1.336  -1.663  -1.393  -1.086
53    D        -1.125  -0.695  -1.052  -0.776  -0.250  -0.731  -1.143  -0.627  -1.015
54    D         1.036   1.065   1.124   1.227   1.260   1.337   1.064   1.115   1.198
55    E        -0.730  -0.926  -1.069  -0.236  -0.489  -0.724  -0.624  -0.872  -1.035
56    V         0.132   0.064  -0.202   0.150   0.062  -0.286   0.155   0.057  -0.371
57    L        -0.552  -0.517  -0.291  -0.682  -0.572  -0.241  -0.884  -0.858  -0.518
58    V         0.132  -0.061  -0.209   0.150  -0.102  -0.297   0.155  -0.123  -0.382
59    V        -1.541  -1.557  -1.673  -1.294  -1.382  -1.451  -1.273  -1.307  -1.353
60    R         1.036   1.065   1.124   0.893   0.925   1.065   1.064   1.115   1.198
61    G         1.036   1.065   1.124   1.227   1.273   1.337   1.064   1.115   1.198
62    S         0.132  -0.002  -0.211  -0.038  -0.199  -0.474   0.155  -0.037  -0.385
63    K        -1.125  -1.289  -1.114  -1.284  -1.397  -1.035  -1.143  -1.352  -1.090
64    K         1.036   1.065   1.124   0.639   0.730   0.858   1.064   1.115   1.198
65    G         0.132  -0.061  -0.209   0.475   0.227  -0.051   0.155  -0.123  -0.382
66    Q        -2.135  -2.046  -1.711  -2.319  -2.315  -1.874  -1.923  -1.801  -1.389
67    E         1.036   1.065   1.124   1.215   1.256   1.326   1.064   1.115   1.198
68    G         1.036   1.065   1.124   1.227   1.273   1.337   1.064   1.115   1.198
69    K         1.036   1.065   1.124   0.639   0.730   0.858   1.064   1.115   1.198
70    I        -0.552  -0.423  -0.292  -0.830  -0.651  -0.417  -0.884  -0.698  -0.520
71    S        -1.125  -1.029  -1.096  -1.201  -1.114  -1.011  -1.143  -1.101  -1.070
72    S         0.132  -0.002  -0.211   0.001  -0.136  -0.366   0.155  -0.037  -0.385
73    V         1.036   1.065   1.124   0.907   0.946   1.076   1.064   1.115   1.198
74    Y         1.036   1.065   1.124   1.397   1.425   1.475   1.064   1.115   1.198
75    R         1.036   1.065   1.124   0.893   0.925   1.065   1.064   1.115   1.198
76    L         0.132   0.064  -0.202   0.138   0.082  -0.459   0.155   0.057  -0.371
77    K         1.036   1.065   1.124   0.639   0.730   0.858   1.064   1.115   1.198
78    F         0.132  -0.061  -0.209   0.697   0.439   0.157   0.155  -0.123  -0.382
79    A        -1.978  -1.981  -1.689  -2.252  -2.295  -1.843  -1.793  -1.742  -1.369
80    V        -1.303  -1.245  -1.095  -1.403  -1.315  -1.121  -1.403  -1.307  -1.069
81    Q        -1.125  -1.213  -1.098  -0.783  -0.873  -0.841  -1.143  -1.237  -1.072
82    V        -0.730  -0.663  -1.040  -0.628  -0.556  -1.081  -0.624  -0.557  -1.001
83    D        -1.303  -1.296  -1.095  -1.114  -1.092  -0.807  -1.403  -1.362  -1.068
84    K        -1.125  -1.289  -1.114  -1.622  -1.916  -1.606  -1.143  -1.352  -1.090
85    V        -0.552  -0.299  -0.258  -0.952  -0.519  -0.479  -0.884  -0.493  -0.463
86    T        -1.125  -1.042  -1.078  -0.749  -0.678  -0.867  -1.143  -1.018  -1.047
87    K        -0.314  -0.421  -0.293  -0.818  -1.005  -0.767  -0.494  -0.693  -0.521
88    E        -0.314  -0.444  -0.297  -0.144  -0.396  -0.213  -0.494  -0.732  -0.527
89    K         1.036   1.065   1.124   0.639   0.730   0.858   1.064   1.115   1.198
90    V        -1.303  -1.045  -1.093  -1.697  -1.281  -1.297  -1.403  -1.131  -1.066
91    N        -0.730  -1.031  -1.077  -0.187  -0.662  -0.865  -0.624  -1.004  -1.045
92    G         1.036   1.065   1.124   1.227   1.273   1.337   1.064   1.115   1.198
93    A         1.036   1.065   1.124   1.079   1.121   1.216   1.064   1.115   1.198
94    S         0.132  -0.002  -0.211  -0.038  -0.199  -0.474   0.155  -0.037  -0.385
95    V         0.132   0.064  -0.202   0.133   0.041  -0.334   0.155   0.057  -0.371
96    P         1.036   1.065   1.124   1.316   1.347   1.409   1.064   1.115   1.198
97    I        -1.303  -1.026  -1.093  -1.322  -1.084  -0.998  -1.403  -1.118  -1.066
98    N        -1.125  -1.045  -1.098  -0.728  -0.733  -0.829  -1.143  -1.115  -1.072
99    L        -1.125  -1.012  -1.069  -0.907  -0.697  -0.850  -1.143  -0.980  -1.035
100   H        -0.730  -0.926  -1.069  -0.045  -0.332  -0.630  -0.624  -0.872  -1.035
101   P        -0.314  -0.395  -0.291  -0.094  -0.289  -0.216  -0.494  -0.650  -0.519
102   S         1.036   1.065   1.124   0.736   0.794   0.937   1.064   1.115   1.198
103   K         0.132  -0.061  -0.209  -0.092  -0.277  -0.422   0.155  -0.123  -0.382
104   L        -0.314  -0.263  -0.281  -0.525  -0.459  -0.521  -0.494  -0.435  -0.501
105   V         0.132  -0.002  -0.211   0.118  -0.060  -0.391   0.155  -0.037  -0.385
106   I         1.036   1.065   1.124   1.195   1.229   1.311   1.064   1.115   1.198
107   T         1.036   1.065   1.124   1.360   1.376   1.445   1.064   1.115   1.198
108   K         0.132  -0.002  -0.211  -0.189  -0.337  -0.698   0.155  -0.037  -0.385
109   L         1.036   1.065   1.124   0.974   1.059   1.130   1.064   1.115   1.198
110   H        -0.730  -1.031  -1.077  -0.134  -0.622  -0.856  -0.624  -1.004  -1.045
111   L         1.036   1.065   1.124   0.974   1.059   1.130   1.064   1.115   1.198
112   D         1.036   1.065   1.124   1.227   1.260   1.337   1.064   1.115   1.198
113   K         0.132  -0.002  -0.211  -0.137  -0.263  -0.548   0.155  -0.037  -0.385
114   D         1.036   1.065   1.124   1.227   1.260   1.337   1.064   1.115   1.198
115   R         1.036   1.065   1.124   0.893   0.925   1.065   1.064   1.115   1.198
116   K         0.132  -0.061  -0.209  -0.115  -0.310  -0.486   0.155  -0.123  -0.382
117   A        -1.541  -1.319  -1.647  -1.185  -0.931  -1.421  -1.273  -1.047  -1.328
118   L         0.132  -0.061  -0.209   0.217   0.009  -0.248   0.155  -0.123  -0.382
119   I         0.132  -0.061  -0.209   0.407   0.136  -0.174   0.155  -0.123  -0.382
120   Q        -1.541  -1.844  -1.705  -1.047  -1.579  -1.596  -1.273  -1.596  -1.383
121   R         1.036   1.065   1.124   0.893   0.925   1.065   1.064   1.115   1.198
122   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
123   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
124   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
125   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
126   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
127   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
128   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
129   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
130   K         0.132   0.338  -0.184  -0.165   0.131  -0.582   0.155   0.423  -0.341
131   G        -0.730  -1.031  -1.077  -0.325  -0.772  -0.969  -0.624  -1.004  -1.045
132   G         0.132  -0.061  -0.209   0.494   0.254   0.001   0.155  -0.123  -0.382
133   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
134   K        -1.541  -1.682  -1.683  -1.591  -1.809  -1.672  -1.273  -1.441  -1.362
135   L        -2.551  -2.577  -2.179  -2.459  -2.516  -1.967  -2.053  -2.023  -1.570
136   E         0.132  -0.061  -0.209   0.441   0.177  -0.119   0.155  -0.123  -0.382
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P05743
Output conservation file - output/P05743
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
