1     M         1.617   1.658   1.753   1.784   1.834   1.990   1.828   1.892   2.055
2     L        -0.006  -0.045  -0.060  -0.093  -0.139  -0.170  -0.177  -0.215  -0.257
3     R        -0.319  -0.439  -0.538  -0.149  -0.354  -0.514  -0.295  -0.451  -0.570
4     N        -1.892  -1.829  -1.705  -1.741  -1.660  -1.472  -1.406  -1.337  -1.183
5     T        -1.077  -1.162  -1.054  -1.046  -1.103  -0.971  -1.002  -1.059  -0.919
6     F        -1.508  -1.374  -1.385  -1.455  -1.273  -1.258  -1.238  -1.107  -1.059
7     T        -1.820  -1.768  -1.695  -1.727  -1.651  -1.560  -1.356  -1.300  -1.177
8     R        -0.319  -0.393  -0.535  -0.125  -0.228  -0.467  -0.295  -0.401  -0.567
9     A         0.053   0.163   0.005  -0.138   0.017  -0.228  -0.059   0.100  -0.153
10    G        -0.623  -0.622  -0.626  -0.857  -0.862  -0.884  -0.688  -0.681  -0.679
11    G        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
12    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
13    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
14    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
15    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
16    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
17    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
18    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
19    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
20    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
21    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
22    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
23    L        -1.195  -1.150  -1.069  -1.357  -1.326  -1.164  -1.120  -1.057  -0.935
24    S        -1.820  -1.768  -1.695  -1.585  -1.493  -1.407  -1.356  -1.300  -1.177
25    R        -1.390  -1.459  -1.410  -1.203  -1.364  -1.269  -1.120  -1.171  -1.077
26    I        -1.820  -1.809  -1.699  -1.590  -1.619  -1.427  -1.356  -1.325  -1.180
27    T        -1.077  -1.177  -1.081  -1.074  -1.211  -1.076  -1.002  -1.075  -0.945
28    S        -1.195  -1.147  -1.077  -1.390  -1.341  -1.226  -1.120  -1.055  -0.941
29    V         0.291   0.128   0.012   0.478   0.250   0.056   0.295   0.051  -0.141
30    R        -0.570  -0.619  -0.619  -0.614  -0.684  -0.738  -0.648  -0.700  -0.680
31    F        -1.077  -1.177  -1.081  -1.016  -1.195  -1.079  -1.002  -1.075  -0.945
32    A        -0.140  -0.161  -0.119  -0.475  -0.481  -0.410  -0.413  -0.431  -0.360
33    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
34    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
35    -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
36    Q        -1.077  -1.129  -1.059  -1.159  -1.258  -1.157  -1.002  -1.038  -0.925
37    T        -1.077  -1.078  -1.036  -1.123  -1.151  -1.087  -1.002  -0.988  -0.902
38    H        -1.195  -1.139  -1.064  -1.110  -1.039  -0.906  -1.120  -1.054  -0.932
39    A        -0.319  -0.530  -0.590  -0.285  -0.580  -0.648  -0.295  -0.564  -0.636
40    L         0.367   0.427   0.536   0.059   0.169   0.342  -0.059   0.041   0.196
41    S         0.938   0.830   0.758   0.914   0.792   0.636   1.003   0.815   0.646
42    N        -0.570  -0.604  -0.583  -0.454  -0.471  -0.475  -0.648  -0.660  -0.629
43    A         0.424   0.410   0.521   0.047   0.005   0.196   0.059   0.005   0.166
44    A        -1.195  -1.147  -1.077  -1.223  -1.132  -1.055  -1.120  -1.055  -0.941
45    V        -0.334  -0.229  -0.182  -0.567  -0.428  -0.345  -0.648  -0.498  -0.440
46    M        -1.508  -1.482  -1.390  -1.357  -1.340  -1.219  -1.238  -1.194  -1.062
47    D        -0.140  -0.071  -0.108  -0.076   0.015  -0.028  -0.413  -0.337  -0.346
48    L         0.603   0.551   0.577   0.447   0.347   0.379   0.413   0.292   0.283
49    Q        -0.765  -0.642  -0.637  -0.801  -0.617  -0.595  -0.884  -0.739  -0.700
50    S        -0.140  -0.039  -0.113  -0.356  -0.209  -0.274  -0.413  -0.305  -0.352
51    R         1.617   1.658   1.753   1.646   1.703   1.854   1.828   1.892   2.055
52    W         1.617   1.658   1.753   1.755   1.815   1.961   1.828   1.892   2.055
53    E         0.291   0.218   0.042   0.416   0.304   0.050   0.295   0.179  -0.096
54    N        -1.820  -1.782  -1.679  -1.546  -1.488  -1.324  -1.356  -1.308  -1.166
55    M         0.424   0.526   0.568   0.296   0.481   0.507   0.059   0.242   0.265
56    P         0.938   0.830   0.758   1.001   0.858   0.736   1.003   0.815   0.646
57    S        -1.195  -1.220  -1.079  -1.379  -1.360  -1.173  -1.120  -1.115  -0.943
58    T        -0.334  -0.288  -0.174  -0.571  -0.510  -0.328  -0.648  -0.578  -0.427
59    E         0.603   0.694   0.657   0.575   0.706   0.650   0.413   0.569   0.447
60    Q         0.291   0.128   0.012   0.415   0.193  -0.005   0.295   0.051  -0.141
61    Q        -0.883  -1.010  -1.028  -0.723  -0.932  -0.975  -0.766  -0.904  -0.891
62    D        -1.077  -1.088  -1.045  -0.875  -0.913  -0.830  -1.002  -0.998  -0.911
63    I        -0.140  -0.074  -0.111  -0.262  -0.219  -0.202  -0.413  -0.341  -0.350
64    V        -0.140  -0.174  -0.124  -0.172  -0.209  -0.115  -0.413  -0.445  -0.365
65    S        -1.820  -1.797  -1.697  -1.550  -1.470  -1.340  -1.356  -1.318  -1.179
66    K        -1.077  -1.177  -1.081  -1.105  -1.225  -1.103  -1.002  -1.075  -0.945
67    L         1.617   1.658   1.753   1.509   1.564   1.719   1.828   1.892   2.055
68    S        -1.508  -1.527  -1.417  -1.563  -1.518  -1.386  -1.238  -1.229  -1.083
69    E        -0.883  -0.946  -1.017  -0.743  -0.865  -0.988  -0.766  -0.836  -0.880
70    R         1.617   1.658   1.753   1.646   1.703   1.854   1.828   1.892   2.055
71    Q         0.424   0.459   0.543   0.289   0.336   0.466   0.059   0.107   0.212
72    K         0.603   0.694   0.657   0.472   0.632   0.534   0.413   0.569   0.447
73    L        -0.319  -0.530  -0.590  -0.254  -0.561  -0.661  -0.295  -0.564  -0.636
74    P         0.603   0.551   0.577   0.571   0.476   0.508   0.413   0.292   0.283
75    W         1.617   1.658   1.753   1.755   1.815   1.961   1.828   1.892   2.055
76    A        -1.195  -1.137  -1.060  -1.325  -1.267  -1.112  -1.120  -1.052  -0.928
77    Q        -0.765  -0.783  -0.668  -0.799  -0.802  -0.635  -0.884  -0.884  -0.735
78    L         1.617   1.658   1.753   1.509   1.564   1.719   1.828   1.892   2.055
79    T         0.938   0.830   0.758   1.009   0.854   0.744   1.003   0.815   0.646
80    E        -1.195  -1.135  -1.077  -1.248  -1.186  -1.044  -1.120  -1.048  -0.941
81    P        -1.390  -1.315  -1.360  -1.389  -1.316  -1.341  -1.120  -1.046  -1.036
82    E         1.617   1.658   1.753   1.608   1.659   1.817   1.828   1.892   2.055
83    K         1.617   1.658   1.753   1.561   1.639   1.770   1.828   1.892   2.055
84    Q         0.367   0.418   0.527   0.043   0.138   0.290  -0.059   0.021   0.179
85    A         1.617   1.658   1.753   1.443   1.492   1.655   1.828   1.892   2.055
86    V         0.938   1.051   0.790   0.850   0.987   0.666   1.003   1.179   0.707
87    W         0.367   0.418   0.527   0.308   0.395   0.554  -0.059   0.021   0.179
88    Y         0.938   0.830   0.758   1.202   1.076   0.965   1.003   0.815   0.646
89    I         1.617   1.658   1.753   1.684   1.732   1.892   1.828   1.892   2.055
90    S         0.603   0.518   0.570   0.397   0.240   0.262   0.413   0.227   0.269
91    Y         0.367   0.418   0.527   0.305   0.383   0.547  -0.059   0.021   0.179
92    G         1.617   1.658   1.753   1.616   1.675   1.825   1.828   1.892   2.055
93    E        -0.006   0.049  -0.077  -0.075  -0.042  -0.226  -0.177  -0.142  -0.291
94    W         0.603   0.518   0.570   0.748   0.610   0.682   0.413   0.227   0.269
95    G         1.617   1.658   1.753   1.616   1.675   1.825   1.828   1.892   2.055
96    P         1.617   1.658   1.753   1.612   1.665   1.821   1.828   1.892   2.055
97    R         1.617   1.658   1.753   1.646   1.703   1.854   1.828   1.892   2.055
98    R        -0.765  -0.737  -0.680  -0.973  -0.977  -0.873  -0.884  -0.849  -0.748
99    P         0.878   0.734   0.705   0.977   0.783   0.738   0.904   0.641   0.543
100   V        -0.140  -0.200  -0.125  -0.323  -0.404  -0.308  -0.413  -0.474  -0.366
101   L        -0.570  -0.514  -0.579  -0.277  -0.191  -0.320  -0.648  -0.567  -0.626
102   N        -1.077  -1.144  -1.048  -0.983  -1.077  -0.940  -1.002  -1.050  -0.916
103   K         0.386   0.492   0.572   0.083   0.274   0.388  -0.020   0.174   0.273
104   G         1.617   1.658   1.753   1.629   1.694   1.838   1.828   1.892   2.055
105   D        -0.006  -0.117  -0.094   0.108  -0.065  -0.084  -0.177  -0.333  -0.317
106   S        -1.390  -1.110  -1.333  -1.312  -0.854  -1.265  -1.120  -0.827  -1.012
107   S        -0.765  -0.768  -0.675  -1.088  -1.052  -0.933  -0.884  -0.872  -0.744
108   F        -1.077  -0.994  -1.014  -0.975  -0.862  -0.868  -1.002  -0.899  -0.880
109   I         0.367   0.418   0.527   0.177   0.254   0.423  -0.059   0.021   0.179
110   A        -0.570  -0.451  -0.572  -0.543  -0.342  -0.561  -0.648  -0.483  -0.617
111   K        -0.647  -0.686  -0.641  -0.684  -0.698  -0.655  -0.766  -0.781  -0.702
112   G         0.291   0.263   0.049   0.463   0.435   0.143   0.295   0.250  -0.085
113   V        -0.140  -0.162  -0.114  -0.225  -0.270  -0.169  -0.413  -0.434  -0.352
114   A        -1.077  -0.953  -1.015  -1.080  -0.864  -0.964  -1.002  -0.852  -0.880
115   A        -0.570  -0.445  -0.577  -0.719  -0.558  -0.723  -0.648  -0.501  -0.630
116   G         0.938   1.002   0.788   1.059   1.146   0.901   1.003   1.103   0.704
117   L         0.291   0.263   0.049   0.337   0.301   0.009   0.295   0.250  -0.085
118   L        -1.820  -1.794  -1.681  -1.690  -1.679  -1.497  -1.356  -1.316  -1.167
119   F        -1.077  -1.024  -1.014  -1.173  -1.117  -1.063  -1.002  -0.943  -0.884
120   S         0.603   0.589   0.591   0.454   0.450   0.395   0.413   0.368   0.311
121   V        -0.319  -0.181  -0.484  -0.084   0.105  -0.333  -0.295  -0.124  -0.501
122   G        -1.077  -0.924  -1.009  -1.105  -0.843  -1.029  -1.002  -0.824  -0.874
123   L         0.424   0.445   0.529   0.145   0.163   0.301   0.059   0.077   0.183
124   F         1.617   1.658   1.753   1.751   1.806   1.957   1.828   1.892   2.055
125   A        -0.006  -0.040  -0.086  -0.172  -0.258  -0.274  -0.177  -0.258  -0.306
126   V        -1.195  -1.130  -1.064  -1.203  -1.142  -1.006  -1.120  -1.038  -0.933
127   V        -0.883  -0.868  -0.975  -0.529  -0.510  -0.645  -0.766  -0.747  -0.836
128   R         0.938   0.901   0.769   1.031   0.984   0.779   1.003   0.936   0.668
129   M        -0.765  -0.790  -0.675  -0.881  -0.960  -0.748  -0.884  -0.892  -0.743
130   A        -0.570  -0.572  -0.596  -0.530  -0.540  -0.622  -0.648  -0.645  -0.652
131   G        -0.319  -0.189  -0.492  -0.348  -0.175  -0.612  -0.295  -0.129  -0.511
132   G        -1.077  -1.123  -1.064  -1.189  -1.278  -1.211  -1.002  -1.031  -0.930
133   Q        -0.570  -0.444  -0.577  -0.532  -0.354  -0.561  -0.648  -0.468  -0.622
134   D        -0.570  -0.563  -0.595  -0.473  -0.477  -0.493  -0.648  -0.641  -0.651
135   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
136   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
137   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
138   A         0.291   0.326   0.055   0.373   0.414  -0.015   0.295   0.341  -0.076
139   K        -1.077  -1.060  -1.060  -1.113  -1.095  -1.086  -1.002  -0.978  -0.926
140   T         0.938   1.080   0.799   1.009   1.179   0.813   1.003   1.224   0.724
141   M         0.291   0.318   0.058   0.572   0.603   0.205   0.295   0.330  -0.070
142   N        -0.140  -0.118  -0.082  -0.211  -0.173  -0.114  -0.413  -0.354  -0.304
143   K        -0.006  -0.072  -0.087  -0.048  -0.125  -0.160  -0.177  -0.275  -0.306
144   E         1.617   1.658   1.753   1.608   1.659   1.817   1.828   1.892   2.055
145   W         1.617   1.658   1.753   1.755   1.815   1.961   1.828   1.892   2.055
146   Q         1.617   1.658   1.753   1.612   1.671   1.821   1.828   1.892   2.055
147   L         0.424   0.558   0.613   0.138   0.381   0.450   0.059   0.307   0.358
148   K         0.291   0.263   0.049   0.338   0.314  -0.040   0.295   0.250  -0.085
149   S         0.938   0.880   0.765   0.939   0.896   0.704   1.003   0.901   0.661
150   D         0.424   0.410   0.521   0.423   0.379   0.543   0.059   0.005   0.166
151   E         0.938   1.002   0.788   1.032   1.113   0.855   1.003   1.103   0.704
152   Y         0.291   0.172   0.016   0.622   0.471   0.230   0.295   0.118  -0.136
153   L         0.938   0.830   0.758   0.933   0.802   0.716   1.003   0.815   0.646
154   K         1.617   1.658   1.753   1.561   1.639   1.770   1.828   1.892   2.055
155   S        -0.006  -0.131  -0.119  -0.143  -0.342  -0.363  -0.177  -0.371  -0.354
156   K        -0.319  -0.530  -0.590  -0.173  -0.446  -0.561  -0.295  -0.564  -0.636
157   N        -0.006  -0.122  -0.118   0.082  -0.131  -0.152  -0.177  -0.363  -0.353
158   A         0.291   0.110   0.003   0.248  -0.003  -0.170   0.295   0.025  -0.155
159   N         0.938   0.880   0.765   1.182   1.117   0.963   1.003   0.901   0.661
160   P         1.617   1.658   1.753   1.612   1.665   1.821   1.828   1.892   2.055
161   W        -0.647  -0.724  -0.639  -0.532  -0.638  -0.519  -0.766  -0.821  -0.700
162   G        -0.140  -0.146  -0.089  -0.299  -0.282  -0.199  -0.413  -0.382  -0.305
163   G         0.938   0.901   0.769   1.017   0.973   0.784   1.003   0.936   0.668
164   Y         0.291   0.110   0.003   0.571   0.313   0.105   0.295   0.025  -0.155
165   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
166   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
167   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
168   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
169   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
170   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
171   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
172   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
173   S        -0.006  -0.122  -0.118  -0.078  -0.241  -0.239  -0.177  -0.363  -0.353
174   Q         0.938   0.880   0.765   1.051   0.992   0.857   1.003   0.901   0.661
175   V         0.424   0.427   0.525   0.287   0.271   0.418   0.059   0.039   0.173
176   Q         0.938   0.830   0.758   1.001   0.864   0.736   1.003   0.815   0.646
177   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
178   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
179   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
180   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
181   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
182   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
183   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
184   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
185   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
186   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
187   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
188   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
189   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
190   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
191   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
192   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
193   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
194   -        -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000  -1.000     *
195   S         0.053  -0.067  -0.079   0.033  -0.139  -0.178  -0.059  -0.245  -0.283
196   K         0.053  -0.031  -0.075  -0.026  -0.174  -0.282  -0.059  -0.189  -0.276
* gap fraction no less than  0.50; conservation set to M-S
  M: mean;  S: standard deviation

al2co - The parameters are:

Input alignment file - ../mafft-mac/clustal/P00424
Output conservation file - output/P00424
All 9 methods are used - true 
   1. unweighted entropy measure; 2. Henikoff entropy measure;
   3. independent count entropy measure;
   4. unweighted variance measure; 5. Henikoff variance measure;
   6. independent count variance measure;
   7. unweighted matrix-based sum-of-pairs measure;
   8. Henikoff matrix-based sum-of-pairs measure;
   9. independent count matrix-based sum-of-pairs measure;
Window size - 1
Conservation normalized to zero mean and unity variance
Gap fraction to suppress calculation -  0.50
